Adult male rats weighing 250-300 g were used to study ACPase and ATPase in the liver following 60Co radiation on the whole body for a single dose 2000 rad(95.7-99r/min). The irradiated group and the control group were sacrificed at intervals of 1, 2, 3 days after irradiation. The left liver lobe was removed, fro/en in dryice-isopentane, and then cut in a cryostat. ACPase and ATPase activities were revealed by histochemical and cytochemical methods respectively.In the control group, ACPase activity was found to be mainly localized in the vicinity of bile canaliculi, and generally higher in the periportal region than in the centrilobular region. The distribution of ATPase reaction products was much like that of ACPase.On the 1st day after irradiation, a slight decrease was found in ACPase and ATPase activities, while on the 2nd and 3rd days in both of them was found a marked increase especially in the periportal region. These results were in agreement with cytochemical observations.The significance of these results and related problems were discussed.

Autoimmune diseases are characterized by the presence of various autoantibodies and may cause injuries to multiple organs,with kidney as the most common and important organ involved.Autoantibodies are of great importance in the diagnosis,treatment and prognosis of autoimmune renal diseases.Lupus nephritis,anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis and antiglomerular basement membrane (GBM) disease are the most common autoimmune renal diseases.Anti-C1q antibody,ANCA and anti-GBM antibody play important roles in those diseases,respectively.Appropriate and steady detecting methods are crucial to clinicians,and the results should also be interpreted with great cautions.

Application research on human amniotic membrane has been carried out for nearly a hundred years and people found that there were more than dozens of kinds bioactive substances in the amniotic membrane. It has been proved that the amniotic membrane has a lot of functions, such as anti-inflammatory, anti-bacterial, anti-virus, anti-angiogenic and promoting cell apoptosis, and soon. As effective treatments, amniotic membrane has been used for adjunctive therapy of burns, trauma, ophthalmic damage, dermatopathya. Recent advances of amniotic membrane and amniotic membrane-derived cells research have led to enormous progress in skin tissue engineering, vascular tis- sue engineering, biological scaffold material, and biological sustained-release materials. Amniotic membrane and amniotic membrane derived cells have a significant advantage and unique charm in medical field. Therefore, they have higher research value and broad prospects in the applications.
Amnion ; Biomedical Research ; trends ; Humans ; Tissue Engineering ; Treatment Outcome

To search the statistical approaches for evaluating and dealing with the center effects in multi-center clinical trial. Breslow-Day test was used for evaluating the differences of the virtual rating among the centers whose responses were binary, Cochran-Mantel-Haenszel test for dealing with the center effects whose responses were binary or ordinal, and logistic regression for evaluating and dealing with the center effects simultaneously. The results showed that Breslow-Day test is invalid in evaluating the center effects whose response is ordinal, and Cochran-Mantel- Haenszel test can not be used for dealing with the effects of other covariates meanwhile.

Objective To explore the best sampling position of bone marrow biopsy in mice.Methods Mice were sacrificed by cervical dislocation, then take the skull, sternum, tibial, femoral and iliac bone, making pathological section.Observed and photographed under light microscope. Comparison of the distribution of the essence and the hematopoietic microenvironment in different parts.Select site which can best reflect the hematopoietic function of bone marrow.Results A large number of hematopoietic cells in ilium marrow sections visible.The cells are evenly distributed. Blood and megakaryocytes were clearly visible.The arrangement of the structure of the scaffolds for tissue closely.The number of fat cells less.Bone marrow hematopoietic cells in the skull, sternum, tibial, femoral were not clear and not active.And there are more fat cells.Conclusions As the best sampling position of bone marrow biopsy, is ilium.

Objective To investigate the clinical efficacy and safety of montelukast combined with budesonide in the treatment of children with asthma. Methods 70 cases of children with asthma were randomly divided into group A and group B, included 35 cases in each group. Group A was treated with single budesonide and Group B was given montelukast combined with budesonide. The clinical efficacy of two groups of asthma was compared, the body temperature returned to normal time, the time of wheezing disappeared, the normal time of the laboratory index, the normal time of the chest radiograph, the inflammatory factors and the lung function indexes and the side effects. Results The clinical curative effect of group B was higher than that of group A(P<0.05); Body temperature in group B returned to normal time, the time of wheezing disappeared, the normal time of laboratory index was shorter than that of group A(P<0.05); Inflammatory factors and lung function were similar. After intervention, the inflammatory factors and lung function of group B were better than those of group A(P<0.05). There were no obvious side effects in the two groups. Conclusion The clinical efficacy and safety of montelukast combined with budesonide in the treatment of children with asthma can improve the symptoms of children, cause the symptoms to recite in a short time, reduce the inflammatory factors, improve lung function, no obvious adverse reactions, safety effective.

The connotation development of industry and education is the road to advance the development of connotation and the quality of higher vocational and technical education.Based on this and bringing into play the advantages of running a vocational school and combined with cultivating the applied personnel needed by enterprises and professions,the college perfects the personnel cultivation model.carries out the "225" education model,accelerate the development of the teacher,promote its service capacity to enhance constantly the quality of higher vocational and technical education.

As one of active immune effector molecules,immunoglobulins play an important role in the pathogenesis,diagnosis and treatment of autoimmune bullous diseases.Human immunoglobulins can be divided into 5 categories,including IgG,IgA,IgM,IgD and IgE,and all of them participate in and play different roles in the pathogenesis of autoimmune bullous diseases except IgD.IgG can induce the formation of blisters by activating complements and leukocytes as well as by releasing proteolytic enzymes,and different subtypes of IgG differ in biological activities.IgA can cause granulocyte migration and lead to the formation of blisters and pustules.IgE is associated with urticaria-like erythema and eosinophil infiltration.IgM is usually seen in Brazilian pemphigus foliaceus.These findings may provide new ideas for the diagnosis and treatment of autoimmune bullous diseases.

Objective To discuss the clinical application and features of coronary artery bypass grafting(CABG) through left intercostal thoracotomy approach. Methods A total of 36 cases of CABG were performed through left intercostal thoracotomy approach from September 1996 to August 2005 in this hospital.The patients fell into four categories: ①single vessel disease in the left anterior descending artery(LAD);②re-operation of CABG with a patent LIMA(left internal mammary artery)-LAD bypass;③severe calcified ascending aorta;and ④concurrent other procedures that required left thoracotomy.There were 26 cases of single vessel disease,3 cases of double vessel disease,and 7 cases of triple vessel disease.Five cases had a history of percutaneous coronary interventions(PCIs) and 6 cases had a history of CABGs.The left ventricular ejection fraction was 0.562?0.107. Results Off pump CABG with 1~4 distal anastomosis was successfully accomplished in all the 36 cases,with an operation time of 190.4?44.1 min.Concurrent procedures included 2 cases of transmyocardial laser revascularization(TMLR),1 case of lung cancer resection,and 1 case of esophageal cancer resection.There was no in-hospital mortality.Two fatal cases were seen in long-term follow-up observations.Recurrent angina developed in 2 cases 1 year after operation,and was cured with a PCI in 1 case and with a re-operation of CABG in 1 case. Conclusions The left intercostal thoracotomy is a safe and feasible approach for CABG surgery in patients with some special types of coronary heart disease.The procedure can be performed under beating heart.

Objective To report the experience of first 100 cases of off-pump coronary artery bypass(OPCAB) grafting by a training cardiac surgeon,and to discuss the feasibility of the training method.Methods The trainee,who had no previous experience of coronary surgery,received an OPCAB training program under the guidance by a director who had completed more than 2000 cases OPCAB.After 28 months of training,the trainee began to be an independent operator.The data of the first consecutive 100 cases of OPCAB by the trainee(Training Group) were collected for comparison with the director's 100 cases of OPCAB surgery(Control Group).Results There were 5 cases of conversion to on-pump surgery in the Training Group.The number of distal anastmosis in the Training Group(2.9?0.6) was smaller than that in the Control Group(3.5?0.7)(t=-6.508,P=0.001).Compared with the Control Group,there were less circumflex grafts(67/100 vs 88/100;?~2=12.645,P=0.000) and sequential grafts(3/100 vs 27/100;?~2=22.588,P=0.000) in the Training Group.There was no significant difference in mortality between the Training Group(3%,3 cases) and the Control Group(1%,1 case)(?~2=0.255,P=0.614).No significant difference was observed between the two groups in other postoperative severe complications.Conclusions The first 100 cases of OPCAB surgery performed by the trainee give favorable results,indicating a stable transition from training period to independent performance.It also testifies the feasibility of this training program.