The low frequency electrical impulses has a significant effect in pain control.Aportable low frequency therapeutic apparatus has been made in our laboratory This paper presents the structure,principle,specification and application of our therapeutic apparatus (including both mono-impulse and train-impulse models) as well as the results of its clinical application.

Objective To elucidate the significance of expressive change in tissues of breast carcinomas.Methods Expression of PTEN protein was detected by immunohistochemistry (SP) in breast carcinomas?atypical intraductal hyperplasia?normal breast tissue. Results The positive rate and expressive intensity of PTEN protein in breast carcinoma were lower and weaker than those in atypical intraductal hyperplasia and normal breast. The intensity of PTEN protein expression was associated with differentiation degree of breast carcinoma, the poorer differentiation of breast carcinoma , the weaker expression of PTEN protein. Comparing with breast carcinoma, the PTEN expression level of atypical intraductal hyperplasia was higher , but It was lower than normal breast tissue. it is helpful for us to assess the carcinogenesis probability of atypical intraductal hyperplasia by detecting the PTEN.

Animals ; Cadherins ; metabolism ; Carcinoma, Hepatocellular ; metabolism ; pathology ; Catenins ; metabolism ; Epithelial-Mesenchymal Transition ; drug effects ; genetics ; Humans ; Liver Neoplasms ; metabolism ; pathology ; MicroRNAs ; genetics ; metabolism ; Transforming Growth Factor beta ; pharmacology

BACKGROUND:Many studies have shown that different nanostructures produce different influences on cellbioactivity, but the nanonetwork structure is not reported yet. OBJECTIVE:To study the influence of the nanonetwork topography on the bioactivity of bone marrow mesenchymal stem cels. METHODS:The nanonetwork topography was fabricated on biomedical titanium surface by alkali-heat treatment, and pure titanium served as control group. Bone marrow mesenchymal stem cels were co-cultured with the above two types of samples. cellmorphology and cytoskeleton were observed using scanning electron microscope and immunofluorescence method. The celladhesion, proliferation and osteogenic differentiation were detected by measurement of absorbance values at different culture time. RESULTS AND CONCLUSION: The nanonetwork topography had significant advantage on the number of adherent cels at 30, 60 and 120 minutes of co-culture. The cellproliferation was significantly accelerated by the nanonetwork topography at days 1, 3, 5 of co-culture, and the absorbance values in the nanonetwork group were significantly higher than those in the pure titanium group (P < 0.05). The alkaline phosphatase activity in the nanonetwork group was also significantly higher than that in the pure titanium group at 14 days of osteogenic induction (P < 0.05). The cellshape and cytoskeleton on the nanonetwork surface were better than those on the titanium surface. These findings indicate that the nanonetwork topography has better effects on cellbioactivity compared with pure titanium.

BACKGROUND:Autologous cartilage has a poor self-repair effect due to low chondrocyte density, low metabolism rate and no blood supply.
OBJECTIVE:To summarize the recent study about tissue engineering techniques and surgical treatment for cartilage injury.
METHODS:A computer-based online retrieval of PubMed database was performed by the first author for articles published between January 1992 and December 2013. The key words are“articular cartilage, injury, tissue engineering, repair”in English. According to the inclusion and exclusion criteria, 61 literatures were included into the final analysis.
RESULTS and CONCLUSION:The current clinical treatment of articular cartilage injury includes joint debridement, mosaicplasty, perichondrium transplantation and autologous chondrocyte implantation. However, their long-term result is unsatisfactory. One reason for limited clinical success is that new cartilage can be formed at the site of a defect, and the repaired tissue canot compare with the autologous cartilage in mechanical property. Tissue engineering technique is stil a hot topic in recent years, because it can potential y induce autologous cartilage formation. Through endogenous or ectogeneous seed cells and inducting factor and nutrient factors, tissue engineering technique can be applied to induce the self-repair of articular cartilage, thus regenerating into hyalinc cartilage with the similar even same biological property. How to simplify the treatment protocols and reduce treatment cost is the key to promote cartilage repair.

OBJECTIVETo investigate the epidemiological characteristics, clinical manifestations, diagnosis, and treatment of Brucella orchitis, so as to provide reliable evidence for the prevention and treatment of the disease.

METHODSWe conducted retrospective statistical analyses on the medical records of 48 outpatients and 21 inpatients with Brucella orchitis.

RESULTSBrucella orchitis was diagnosed in 6.67% of the male patients with brucellosis (69/1 034). The disease exhibited typical epidemiological features, with a higher incidence rate among those in frequent contact with sheep and elderly people, in the period from April to July, and in the areas with sheep husbandry. All the Brucella orchitis patients had such local symptoms as testicular pain and swelling, more frequently involving both testes, and other most common symptoms included fever, chills, sweating, and painful joints. Based on IIEF-5, 45 of the patients suffered from severe erectile dysfunction, with their reproductive function temporarily affected in the course of the disease. Misdiagnosis easily occurred in the early stage of the disease. Therapeutic options mainly included doxycycline hydrochloride and rifampicin, administered orally or intravenously, which could effect a cure, though relapse might occur in some cases.

CONCLUSIONBru- cella orchitis has distinct epidemiological characteristics, with clinical manifestations of testicular pain and swelling. Though a transient disease, it affects the reproductive function of the patient before cured. It can be treated by combined oral and intravenous medication, with painkillers or ice bags for testicular pain and swelling.

Animals ; Brucella ; pathogenicity ; Brucellosis ; complications ; diagnosis ; therapy ; Humans ; Male ; Orchitis ; diagnosis ; microbiology ; therapy ; Retrospective Studies ; Sheep

Objective: To study the interpersonal interaction of junior high school students and its relationship with self-consciousness. Methods: 265 junior high school students were given the measurement of interpersonal relationship, and administered the questionnaire of Piers-Harris children self-consciousness one week later. Results: ①The interpersonal relationship of junior high school students was interactive. ②There were statistically significant differences among some dimensions of self-consciousness of junior high school students of different grades and genders. ③Intellgence and school performance had important effect on personal acceptance of junior high school students. Conclusion: There is interpersonal interaction among junior high school students, and intelligence and school performance influence the personal acceptance significantly.

[Objective]To study the clinical results of methods of improvement of pedicle screw stability in patients of osteoporosis. [Method]Thirty-four cases of patients with osteoporosis were operated using pedicle screw and methods of improvement of pedicle screw stability were used during the operations.Of which,male 20,female 14;the mean age of 57(range 40～71).According to Jikei grading scale for osteoporosis,8 were of early stage,11 of stageⅠ,8 of stageⅡ and 7 of stageⅢ.There were 14 cases of fractures and 20 cases of osteopathy.For patients of early stage and stageⅠ(19 cases),long and large size of pedicle screws were used,meanwhile stiff connection rod system,two cross-link devices,placement of pedicle screw with large angles in horizontal and sagital planes were applied.For patients of stageⅡ and stageⅢ(15 cases) bone cement was used to fill the pedicle hole to ensure the screw stabily.[Result]There were no neurologic and vessel injuries or aggravated as well as no breakage of screw except two cases with loosening of screw during the follow-up with the mean period of 14 months(range 9～26).The loss of correction of reduction in fractures was 5%,the rate of fusion was 100% in grafting.[Conclusion]Different methods of improvement of pedicle screw stability used in osteoporosis may avoid loosening of screw and loss of correction.

Objective To compare and analyze the clinical effects and reliabilities of intermandibular flexible distraction and rigid internal fixation in treating mandibular condylar fractures. Methods Of 40 patients with mandibular condylar fractures, 21 cases were managed with intermandibular flexible distraction (external fixation group, Group A) and 19 with rigid titanium internal fixation (internal fixation group, Group B). There were 15 cases with high position fractures and 25 with low position ones. Results During 2-4 weeks postoperatively, X-rays confirmed the condylar fracture healing in all cases with no loosened mini-tinum plates in the Group B. There were 10 cases in the Group A and 14 cases in the Group B with the opening width over 30 mm, and 11 cases in the Group A and 5 in the Group B with the opening width less than 30 mm. Conclusions Both external and internal fixations can attain condylar fracture healing. However, compared with the internal fixation, the reduction of external fixation is less exact and the function recovery slower after operation, especially in the low position fractures. No irreversible damages on TMJ happen in all cases.

Objective To study the method of surgical treatment for patients with postpancreatic vascular invasion by pancreatic head carcinoma. Methods 25 patients with pancreatic head cancer and 1 with recurrent duodenal carcinoma with postpancreatic vessels involved by malignancy were treated by radical pancreatoduodenectomy with involved vascular resection(RPDIVR). Results There was no operation death in the 26 patients.Of the 26 cases, 4 had pancreatic fistula and 2 bile fistula postoperatively, but no vascular complication occurred. All of the 26 patients were followed up for 2.5-6.5 years( median time 4.5 years) and the average survival time was 56 months with 96.1%,88.4%, and 57.5% of 1,3,5 year survival rate. Coclusions (1)Colour Dopper imaging is a more useful and practical method in the preoperative examination of the vessels involved by malignancy. (2)When the postpancreatic vessels are involved by pancreatic head carcinoma, the rational treatment is RPDIVR. RPDIVR is a useful, safe, and an effective method and can improve the 5 year survival.