The low frequency electrical impulses has a significant effect in pain control.Aportable low frequency therapeutic apparatus has been made in our laboratory This paper presents the structure,principle,specification and application of our therapeutic apparatus (including both mono-impulse and train-impulse models) as well as the results of its clinical application.

Objective To elucidate the significance of expressive change in tissues of breast carcinomas.Methods Expression of PTEN protein was detected by immunohistochemistry (SP) in breast carcinomas?atypical intraductal hyperplasia?normal breast tissue. Results The positive rate and expressive intensity of PTEN protein in breast carcinoma were lower and weaker than those in atypical intraductal hyperplasia and normal breast. The intensity of PTEN protein expression was associated with differentiation degree of breast carcinoma, the poorer differentiation of breast carcinoma , the weaker expression of PTEN protein. Comparing with breast carcinoma, the PTEN expression level of atypical intraductal hyperplasia was higher , but It was lower than normal breast tissue. it is helpful for us to assess the carcinogenesis probability of atypical intraductal hyperplasia by detecting the PTEN.

Animals ; Cadherins ; metabolism ; Carcinoma, Hepatocellular ; metabolism ; pathology ; Catenins ; metabolism ; Epithelial-Mesenchymal Transition ; drug effects ; genetics ; Humans ; Liver Neoplasms ; metabolism ; pathology ; MicroRNAs ; genetics ; metabolism ; Transforming Growth Factor beta ; pharmacology

BACKGROUND:Many studies have shown that different nanostructures produce different influences on cellbioactivity, but the nanonetwork structure is not reported yet. OBJECTIVE:To study the influence of the nanonetwork topography on the bioactivity of bone marrow mesenchymal stem cels. METHODS:The nanonetwork topography was fabricated on biomedical titanium surface by alkali-heat treatment, and pure titanium served as control group. Bone marrow mesenchymal stem cels were co-cultured with the above two types of samples. cellmorphology and cytoskeleton were observed using scanning electron microscope and immunofluorescence method. The celladhesion, proliferation and osteogenic differentiation were detected by measurement of absorbance values at different culture time. RESULTS AND CONCLUSION: The nanonetwork topography had significant advantage on the number of adherent cels at 30, 60 and 120 minutes of co-culture. The cellproliferation was significantly accelerated by the nanonetwork topography at days 1, 3, 5 of co-culture, and the absorbance values in the nanonetwork group were significantly higher than those in the pure titanium group (P < 0.05). The alkaline phosphatase activity in the nanonetwork group was also significantly higher than that in the pure titanium group at 14 days of osteogenic induction (P < 0.05). The cellshape and cytoskeleton on the nanonetwork surface were better than those on the titanium surface. These findings indicate that the nanonetwork topography has better effects on cellbioactivity compared with pure titanium.

Objectives To investigate the associations of serum superoxide dismutase ( SOD) , malondialdehyde (MDA), and uric acid (UA) with blood pressure and the prevalence of hypertension in a Chinese population. Methods We measured serum superoxide dismutase (SOD), malondialdehyde (MDA) and uric acid (UA) in 506 randomly selected participants. For database management and statistical analysis, we used the SAS 9.13 Software. Results Compared with normotensive participants, hypertensive patients had significantly lower serum levels of superoxide dismutase activity ( =0.0002), and higher serum levels of malondialdehyde and uric acid ( <0.05) .Univariate analysis indicated that serum superoxide dismutase activity was negatively associated with blood pressure, and serum levels of uric acid (P 0.0001);while serum levels of malondialdehyde was positively associated with blood pressure, and serum levels of uric acid (P 0.05) . There was significant difference in systolic (Ptrend=0.02) and diastolic blood pressure (Ptrend=0.04) between three groups defined by tertiles of serum levels of superoxide dismutase, with a lower blood pressure in the highest tertile than in the lowest tertile. Furthermore, the systolic (Ptrend=0.01) and diastolic blood pressure (Ptrend=0.003) in the highest tertile, which defied by the serum levels of malondialdehyde, were significantly higher than those in the lowest tertile. Conclusions Hypertensive patients have a disturbed oxidative and anti-oxidative balance. Oxidative stress may play a role in the pathogenesis of hypertension in Chinese.

BACKGROUND:Autologous cartilage has a poor self-repair effect due to low chondrocyte density, low metabolism rate and no blood supply.
OBJECTIVE:To summarize the recent study about tissue engineering techniques and surgical treatment for cartilage injury.
METHODS:A computer-based online retrieval of PubMed database was performed by the first author for articles published between January 1992 and December 2013. The key words are“articular cartilage, injury, tissue engineering, repair”in English. According to the inclusion and exclusion criteria, 61 literatures were included into the final analysis.
RESULTS and CONCLUSION:The current clinical treatment of articular cartilage injury includes joint debridement, mosaicplasty, perichondrium transplantation and autologous chondrocyte implantation. However, their long-term result is unsatisfactory. One reason for limited clinical success is that new cartilage can be formed at the site of a defect, and the repaired tissue canot compare with the autologous cartilage in mechanical property. Tissue engineering technique is stil a hot topic in recent years, because it can potential y induce autologous cartilage formation. Through endogenous or ectogeneous seed cells and inducting factor and nutrient factors, tissue engineering technique can be applied to induce the self-repair of articular cartilage, thus regenerating into hyalinc cartilage with the similar even same biological property. How to simplify the treatment protocols and reduce treatment cost is the key to promote cartilage repair.

Hepatic fibrosis is the most important pathologic course for chronic hepatic injury developing into hepatic cirrhosis. It is meaningful for treating liver disease by inhibiting the development of hepatic fibrosis. Recently, herbs of promoting blood circulation for removing blood stasis has their unique characteristics and exhibits great therapeutic effect on hepatic fibrosis. The review explains the function and mechanism of the promoting blood circulation for removing blood stasis herbs in treating hepatic fibrosis from the aspects of the monomer, effective constituent and compound recipe. It provides basis for application of promoting blood circulation for removing blood stasis herbs in study and treating hepatic fibrosis.

Objective To study the applicability of ultrafiltration of tween-80 and it solubilizing schizonepeta volatile oil and paeonol of ultrafiltration. Method Taking components content as index, temperature, pressure, concentration and membrane pore size were selected as factors for orthogonal design method. The concentration was determinated in medical solution before and after ultrafiltration, and the transmittance rate (TS) was calculated to observe the content variation of tween-80 and it solubilizing solution. Results The four factors had markedly effect on TS of tween-80, concentration was statistically significant (P

[Objective]To study the clinical results of methods of improvement of pedicle screw stability in patients of osteoporosis. [Method]Thirty-four cases of patients with osteoporosis were operated using pedicle screw and methods of improvement of pedicle screw stability were used during the operations.Of which,male 20,female 14;the mean age of 57(range 40～71).According to Jikei grading scale for osteoporosis,8 were of early stage,11 of stageⅠ,8 of stageⅡ and 7 of stageⅢ.There were 14 cases of fractures and 20 cases of osteopathy.For patients of early stage and stageⅠ(19 cases),long and large size of pedicle screws were used,meanwhile stiff connection rod system,two cross-link devices,placement of pedicle screw with large angles in horizontal and sagital planes were applied.For patients of stageⅡ and stageⅢ(15 cases) bone cement was used to fill the pedicle hole to ensure the screw stabily.[Result]There were no neurologic and vessel injuries or aggravated as well as no breakage of screw except two cases with loosening of screw during the follow-up with the mean period of 14 months(range 9～26).The loss of correction of reduction in fractures was 5%,the rate of fusion was 100% in grafting.[Conclusion]Different methods of improvement of pedicle screw stability used in osteoporosis may avoid loosening of screw and loss of correction.

A nutrition survey of 170 pregnant women of the Han, Miao and Buyi nationality in the middle and late pregnancy was conducted in 1984. The survey included dietary survey, physical and obstetrical examination, biochemical analysis and measurement of weight and height of the newborns of the women studied. The results showed that their calorie intake was substantially suficient to the RDA but the protein intake was inadequate. The proportion of high quality protein was more than 30% of total protein intake for the Han, but less than 25% for the other two nationalities. The calcium and vitamin B2 intakes were much lower than RDA for all the women studied. The retinol intake was only about 73% of RDA for the Han in the late pregnancy. The incidence of nutritional anaemia was 26.92-50.00% among the 170 pregnant women. The weight-height of the newborns has no significant difference among three nationalities and all indices of physical development status were in medium rank on an average.