Objective; To elucidate the mechanism of the role of ER-a in fat metabolism by regulating the expression of ER-a in SD rats marrow mesenchymal stem cells during differentiating into adipocytes in vitro. Methods ;SD rats marrow mesenchymal stem cells were separated and cultivated. The ER-a was transfected into the rBMSCs in group one. Tamoxifen Citrate was used to restrain the expression of ER-a in group two, the group without any treatment was used as control. Western blot was used to identify the difference of ER-a expression among different groups and Oil-Red-0 staining was employed to identify the adipocytes in vitro. Results; There was significant difference between the number of lipids and different groups (P <0. 01) ,the number of lipid droplet changed concomitantly with ER-a: Low-expression ER-a group > Control group > High-expression ER-a group. Conclusion; ER-a in SD rats marrow mesenchymal stem cells may restrain the adipocyte differentiation.

Objective:To study the difference of upper airway form and hyoid position of the adults with different vertical facial types.Methods:Cephalograms of 120 adults with different vertical facial types were retrospectively investigated by computerized cephalometric analysis.Upper airway depths and hyoid position were measured,and differences were compared between male and female,also compared among different vertical facial type groups.Results:The sagittal depth of upper airway in male was larger than that in female,but there was no statistical difference except V-LPW.Among different vertical facial types,the sagittal depths of upper airway in high angle group was smaller than that in average angle group and low angle group.There was statistical difference in hyoid position between genders,AH-SN,AH-FH and AH-C3 in male were larger than in female(P

Objective: To study the relation of condylar morphology with different vertical facial types in the development of patients with skeletal Ⅲ malocclusions. Methods:180 cases with skeletal Ⅲ malocclusion were divided into children(5-11 years old), adolescence(12-17 years old) and adult(18-30 years old) groups with 60 cases in each group. Orthopantomograms of the patients with different vertical facial types were retrospectively investigated by computerized cephalometric analysis. Condylar morphology were compared among different age groups of the same vertical facial type. Results:In patients with high angle, ramus height(RH) was getting bigger with ageing(P0.05). In patients with low angle h and RH in adult group were bigger than those in adolescence or in children(P

Child ; Humans ; Medicine, Chinese Traditional ; methods ; Pediatrics

Centric Relation ; Dental Occlusion, Centric ; Humans ; Orthodontics ; Temporomandibular Joint

Objective To evaluate the curative effect of stereotactic radiotherapy in pancreatic cancer in the elderly. Methods Retrospectively analyzed 65 elderly patients with pancreatic cancer were treated with stereotactic radiotherapy. The ages of patients were 60 to 86, with mean age of 71 2. The numbers of treatment were 8 to 12 times according to the tumor size and patients condition Fractional dose were 3 to 6 Gy, total dose were 32 to 54 Gy. The curve of isodose was 50%～80% Results The patients average alive time was 9 3 months after stereotactic radiotherapy The clinical benefit response rate was 61 2%. The CR+PR was 38 4% Conclusions The stereotactic radiotherapy is safe and effective method for treating elderly pancreatic cancer

Objective:To study the changes of iNOS expression in periodontal tissues of rats with periodontitis in orthodontic tooth movement. Methods:48 SD rats at 10 weeks were divided randomly into two groups: periodontitis teeth group and normal teeth group. The maxillary first molar was pulled mesially both in the two groups. 4 rats were executed each time after activation of pull-spring 1, 2, 3, 7,14 and 21d both in two groups. iNOS immunohistochemistry staining were done to compare the expression variations. Results: There were significant differences between the two groups in 2,3, 7, 14 and 21 d. The periodontitis teeth group had stronger positive signals. Conclusion: Periodontitis will increase the expression of iNOS in the rats periodontal tissue during tooth movement, and influence the reconstruction of periodontium.

Objective To explore radionuclide bone imaging characteristics of lung cancer patients and evaluate the clinical significance of bone imaging in the diagnosis of lung cancer patients with bone metastases.Methods One hundred and seventy-seven patients with diagnosed lung cancer were examined by 99mTc -MDP bone imaging,and the symptoms,sites,quantity and pathological types of bone metastases were reviewed and analyzed.Results Total bone metastases ratio of lung cancer was 45.8%(81/177),and 85.2 % (69/81) patients had multiple bone metastases.There was a statistical significance of bone metastases in adenocarcinoma patients (69.0%,40/58),compared with patients of other types of lung cancer[squamous cell cancer (41.3%,19/46),small cell cancer ( 32.5%,13/40),undifferentiated carcinoma ( 27.3%,9/33)](P＜0.05).Bone metastases happened in 526 places: thoracic bones 212 places (40.3%),vertebras 168 places (31.9%),pelvic bones 73 places (13.9%),extremities 62 places (11.8%),and skull 11 places (2.1%).Conclusions Multiple bone metastases is more frequent in lung cancer patients.The most frequent metastatic sites are thoracic bones and vertebras.Bone metastases of adenocarcinoma is more easily than other types of lung cancer.All the patients with lung cancer should perform radionuclide bone scan,which is significant for staging,selecting therapy methods and the evaluating prognosis of the disease.

Objective: To study the difference in tooth profile among Chinese,Caucasian and Japanese with normal occlusion.Methods: The data of the measurments of Chinese,Caucasian and Japanese with normal occlusion were collected from published studies.Crown angulation,crown inclination and crown convex were used in the comparation among the peoples. Results: Tooth profile in Chinese was significantly different from that of Caucasian but was similar to that of Japanese. Conclusion: The orthodontic appliances should be modified when we use the straight wire appliances that is suitable for Caucasian and Japanese.

Objective To study the influence of applied times of Batroxobin on neuroprotective effects in cerebral ischemic-reperfusion(IR) injury gerbils.Methods 45 gerbils were randomly divided into five groups:normal group;cerebral IR injury model group(IR group);three times group;five times group;seven times group.The later three groups were administered Batroxobin 8 U/kg by abdominal injection q.o.d with certain times respectively.The normal group and IR group were not given drug but isometric physiological saline.Flow cytometer was used to measure apoptotic neurons of the hippocampal CA1,meanwhile electronic microscope was used to detect the ultrastrctural change of the hippocampal CA1 region.Result The quantity of apoptotic neurons in the every Batroxobin groups were significantly less than those in the IR groups(all P0.05).The ultrastrctural changes in five times group were lighter than those in three times group,but no significant difference was found between five times group and seven times group.Conclusion Three times,five times and seven times of Batroxobin can reduce the number of apoptotic neurons after IR injury.However,applied five times and seven times of Batroxobin has stronger neuroprotective effect than three times.