Child ; Humans ; Medicine, Chinese Traditional ; methods ; Pediatrics

Objective; To elucidate the mechanism of the role of ER-a in fat metabolism by regulating the expression of ER-a in SD rats marrow mesenchymal stem cells during differentiating into adipocytes in vitro. Methods ;SD rats marrow mesenchymal stem cells were separated and cultivated. The ER-a was transfected into the rBMSCs in group one. Tamoxifen Citrate was used to restrain the expression of ER-a in group two, the group without any treatment was used as control. Western blot was used to identify the difference of ER-a expression among different groups and Oil-Red-0 staining was employed to identify the adipocytes in vitro. Results; There was significant difference between the number of lipids and different groups (P <0. 01) ,the number of lipid droplet changed concomitantly with ER-a: Low-expression ER-a group > Control group > High-expression ER-a group. Conclusion; ER-a in SD rats marrow mesenchymal stem cells may restrain the adipocyte differentiation.

Objective:To study the difference of upper airway form and hyoid position of the adults with different vertical facial types.Methods:Cephalograms of 120 adults with different vertical facial types were retrospectively investigated by computerized cephalometric analysis.Upper airway depths and hyoid position were measured,and differences were compared between male and female,also compared among different vertical facial type groups.Results:The sagittal depth of upper airway in male was larger than that in female,but there was no statistical difference except V-LPW.Among different vertical facial types,the sagittal depths of upper airway in high angle group was smaller than that in average angle group and low angle group.There was statistical difference in hyoid position between genders,AH-SN,AH-FH and AH-C3 in male were larger than in female(P

Objective: To study the relation of condylar morphology with different vertical facial types in the development of patients with skeletal Ⅲ malocclusions. Methods:180 cases with skeletal Ⅲ malocclusion were divided into children(5-11 years old), adolescence(12-17 years old) and adult(18-30 years old) groups with 60 cases in each group. Orthopantomograms of the patients with different vertical facial types were retrospectively investigated by computerized cephalometric analysis. Condylar morphology were compared among different age groups of the same vertical facial type. Results:In patients with high angle, ramus height(RH) was getting bigger with ageing(P0.05). In patients with low angle h and RH in adult group were bigger than those in adolescence or in children(P

Centric Relation ; Dental Occlusion, Centric ; Humans ; Orthodontics ; Temporomandibular Joint

One of the most promising antimalarial drugs which are widely used throughout the world is the artemisinin (ARS)and its derivatives,e.g.,artemether,arteether,and artesunate.Their true potential lies in broader anti-disease applications.The mechanism of action of these compounds appears to involve the endoperoxide bridge to produce carbon-centred free radicals.Large clinical studies did not show serious side effects,however,there is a paucity of large-scale clinical trials suitable to detect rare but significant toxicity.Therefore,a final and definitive statement on the safety of artemisinins still cannot be made.In contrast,animal experiments at high doses shown considerable toxicity upon application of artemisinins.In the present review,the authors give a comprehensive overview on toxicity studies in cell culture and in animals (mice,rats,rabbits,dogs,and monkeys)as well as on toxicity reported in human clinical trials.The authors emphasize the current knowledge on neurotoxicity,embryotoxicity, genotoxicity,hemato-and immunotoxicity and cardiotoxicity.Rapid elimination of artemisinins after oral intake represents a relatively safe route of administration compared to delayed drug release after intra-muscular (im ) injection. There are drug-related differences, i.e., intramuscular application of artemether or arteether,but not to artesunate,which is safe and gives good profiles after im administra-tion in severe malaria.It might also be important in determining dose limitations for treatment of other diseases such as cancer.Questions about dosing regimens,safety of long-term use and possible inter-actions with existing therapies and toxicities that might be related to the treatment of tumors should be answered by appropriate clinical and preclinical studies.

Objective To explore radionuclide bone imaging characteristics of lung cancer patients and evaluate the clinical significance of bone imaging in the diagnosis of lung cancer patients with bone metastases.Methods One hundred and seventy-seven patients with diagnosed lung cancer were examined by 99mTc -MDP bone imaging,and the symptoms,sites,quantity and pathological types of bone metastases were reviewed and analyzed.Results Total bone metastases ratio of lung cancer was 45.8%(81/177),and 85.2 % (69/81) patients had multiple bone metastases.There was a statistical significance of bone metastases in adenocarcinoma patients (69.0%,40/58),compared with patients of other types of lung cancer[squamous cell cancer (41.3%,19/46),small cell cancer ( 32.5%,13/40),undifferentiated carcinoma ( 27.3%,9/33)](P＜0.05).Bone metastases happened in 526 places: thoracic bones 212 places (40.3%),vertebras 168 places (31.9%),pelvic bones 73 places (13.9%),extremities 62 places (11.8%),and skull 11 places (2.1%).Conclusions Multiple bone metastases is more frequent in lung cancer patients.The most frequent metastatic sites are thoracic bones and vertebras.Bone metastases of adenocarcinoma is more easily than other types of lung cancer.All the patients with lung cancer should perform radionuclide bone scan,which is significant for staging,selecting therapy methods and the evaluating prognosis of the disease.

Objective To study the best dose of batroxobin that protects forebrain ischemia reperfusion injury in gerbils.Methods 80 gerbils were randomly assigned to receive different dose of batroxobin (1 BU/kg, 2 BU/kg, 4 BU/kg, 8 BU/ kg, 16 U/kg or 32 BU/kg) in treatment group, NS in control group or nothing in sham-operation group. There were 10 gerbils in each group. NS (control group) or batroxobin (treatment group) was given intraperitoneally three hours before establishment of forebrain ischemia reperfusion model. Apoptotic cells were detected by TUNEL technique and the number of apoptotic cells in the hippocampal CA 1 territory was counted using Olympus microscope.Results The apoptotic cells in the treatment groups (8 BU/kg, 16 BU/kg, 32 BU/kg) markedly decreased than those in the control group and the other treatment groups (1 BU/kg, 2 BU/kg, 4 BU/kg) (all P0.05). Conclusions Batroxobin has a role of reducing neuron apoptosis after cerebral ischemia. The best dose of batroxobin that protects forebrain ischemia reperfusion injury in gerbils is 8 BU/kg.

Objective:To study the changes of iNOS expression in periodontal tissues of rats with periodontitis in orthodontic tooth movement. Methods:48 SD rats at 10 weeks were divided randomly into two groups: periodontitis teeth group and normal teeth group. The maxillary first molar was pulled mesially both in the two groups. 4 rats were executed each time after activation of pull-spring 1, 2, 3, 7,14 and 21d both in two groups. iNOS immunohistochemistry staining were done to compare the expression variations. Results: There were significant differences between the two groups in 2,3, 7, 14 and 21 d. The periodontitis teeth group had stronger positive signals. Conclusion: Periodontitis will increase the expression of iNOS in the rats periodontal tissue during tooth movement, and influence the reconstruction of periodontium.

0.05); ② Significant correlation was found between natural head posture and angle SNA, SNB, SN/MP, N S PNS, NSU, NSGn, ANS PNS Gn, NSH( P