BACKGROUND:Implanted site and direction of miniscrew implants, post-implantation movement of implants, contact between tooth and implant after tooth movement al can cause root damage.
OBJECTIVE:To investigate the changes of the root surface, periodontal tissue and osteoprotegerin expression in the periodontal tissue at periods of time after miniscrew implant loading.
METHODTwo beagle dogs were selected. For each dog, miniscrews were implanted into the labial alveolar bone near the roots of the maxil ary second, third, and fourth premolars and the maxil ary second, third, and fourth premolars and first molar. Fourteen miniscrew implants were in one dog, including six in the maxil ary and eight in the mandible;two miniscrews of the maxil ary acted as control group with no loading, and the rest miniscrews were as experimental group under orthodontics force. Two weeks after miniscrew implantation, 150 g force was loaded between two miniscrews with nickel-titanium closed-coil springs. The dogs were sacrificed after miniscrews loading 4 and 8 weeks respectively, the teeth were cut completely with alveolar bone tissue and decalcified to make the histological specimen. The histological changes of the root and periodontal tissue were observed by hematoxylin-eosin staining and expression of osteoprotegrin in the periodontal ligament was also inspected by using immunohistochemistry.
RESULTS AND CONCLUSION:When the miniscrews were adjacent to the roots, absorption lacuna occurred in the alveolar bone;after horizontal force was loaded opposite to the roots, alveolar resorption became much more animate. When the miniscrews contacted with the pericementum, some areas of the cement absorbed seriously and even reached the dentin;when sustaining pressure from the miniscrews, the whole layer of the cement absorbed and the dentin was exposed and began to be absorbed. The osteoprotegrin in the periodontal ligament of control group expressed sharply and its expression was notable at 8 weeks;while the osteoprotegrin of experimental group weakly expressed at 8 weeks, and there was significant divergence between control group and experimental group at 8 weeks (P<0.01). The roots and periodontal tissue were al damaged variously after miniscrew implantation adjacent to the roots. When horizontal orthodontic force was loaded opposite to the roots, the expression of osteoprotegrin was influenced slightly;as the loading period prolonged, pressure dramatical y restrained the expression of osteoprotegrin. When discovering the miniscrew implant adjacent to roors, the miniscrew implant anchorage should not be used to load orthodontical force opposite to the roots but ought to be removed in order to leave the root repair autonomously and avoid further damage.

Objective: To develop an HPLC-DAD method for the simultaneous determination of five active flavonoids (quercetin, kaempferol, apigenin, 3-methoxyl-quercetin, nobiletin) in Centipeda minima (L.) A.Br.et Aschers.Methods: The chromatographic separation was performed on a Diamonsil C18 column (200 mm×4.6 mm,5 μm) with the mobile phase of 0.1% phosphoric acid-acetonitrile with gradient elution at the flow rate of 0.8 ml·min-1.The detection wavelength was set at 360nm,and the column temperature was maintained at 30 ℃.Results: Quercetin, kaempferol, 3-methoxyl-quercetin, apigenin and nobiletin was linear within the range of 0.002 3-0.093 0 μg·μl-1(r=0.999 5) , 0.002 2-0.087 0 μg·μl-1(r=0.999 6),0.002 0-0.079 0 μg·μl-1(r=0.999 8), 0.000 9-0.037 0 μg·μl-1(r=0.999 8) and 0.000 8-0.031 0 μg·μl-1 (r=0.999 9), respectively.The average recovery was 97.66%(RSD=1.17%), 98.33%(RSD=1.16%), 98.63%(RSD=1.10%), 98.40%(RSD=1.52%) and 98.10%(RSD=1.36%)(n=6) , respectively.Conclusion: The method is convenient, accurate and reproducible, which can be used for the quality control of Centipeda minima (L.) A.Br.et Aschers.

Objective To understand the death level and main death causes among residents in the Wansheng Economic De‐velopment Zone to provide the basis for comprehensive prevention and control .Methods According to the ICD‐10 international classification of diseases ,the death causes were classified and coded for statistically analyzing the crude mortality rate ,standardized mortality rate and the death causes constituents in this surveyed population .Results The crude mortality rate of residents in Wan‐sheng Economic Development Zone during 2014 was 650 .57/100 000 and the standardized mortality rate was 413 .35/100 000 .The sequence of death causes was circulatory system disease ,respiratory system disease ,tumor ,injury and poisoning and digestive sys‐tem diseases .The death number for these 5 kinds of death causes accounted for 93 .21% of the total death number .The top five of potential life loss years were injury and poisoning ,tumor ,circulatory system disease ,respiratory system disease and digestive system diseases .The rates of potential years of life loss were 13 .76‰ ,10 .19‰ ,8 .35‰ ,4 .84‰ and 2 .69‰ respectively .Conclusion Chronic noncommunicable diseases ,injury and poisoning become the main causes of death in the Wansheng Economic Development Zone and disease burden .

Acupressure ; Child ; Child, Preschool ; Combined Modality Therapy ; Female ; Humans ; Male ; Moxibustion ; Nocturnal Enuresis ; therapy

The outpatient service system at Shanghai Sixth People's Hospital came into use since April 2009.Data from Jan.2010 to Oct.2010 and interviews with employees of department in question showed a rapid growth of outpatient appointments,a poor handling of outpatients absenteeism,and poor integration among the platforms of the appointments.The development of outpatient services depends on behavioral change of both medical workers and consumers.Some measures could be employed to improve outpatient appointment within the current mechanism.

To construct recombinant reporter plasmids containing different Alpha gene segments and Alpha1-TFEB fusion gene and to evaluate the promoter activity of the Alpha gene. Methods:Promoter regions of the Alpha gene were predicted using a software Primer 0.5. Five Alpha gene segments with different lengths and a normal TFEB gene promoter (pTFEB) were amplified via polymerase chain reaction, and recombinant reporter plasmids containing different Alpha gene segments and a normal TFEB gene pro-moter were constructed. Liposome transfection was used to transfect these vectors into the human embryo kidney 293T cells. The pro-moter activity of the Alpha gene was evaluated via luciferase assay. Meanwhile, the recombinant Alpha1-TFEB plasmid was construct-ed and transfected into the 293T cells. The TFEB expression of the recombinant Alpha1-TFEB plasmid was then detected via Western blot. Results: Recombinant reporter plasmids containing different Alpha gene segments and pTFEB were constructed successfully. Compared with the luciferase activity of pGL3-Basic, that of the groups with Alpha1, Alpha2, Alpha3, Alpha4 and Alpha5 significantly increased (P<0.01). The luciferase activity also increased significantly in the groups with Alpha1, Alpha2 and Alpha5 compared with that of the pTFEB group (P<0.01). The TFEB expression of the pGL3-Enhancer-Alpha1-TFEB was significantly higher compared with that of the pGL3-Enhancer group. Conclusion:In t(6;11) translocation RCC, the Alpha gene has a strong promoter activity and it en-hances TFEB expression. The strongest promoter activity region is in Alpha5 with a sequence from 643 bp to 693 bp.

BCG Vaccine ; adverse effects ; Humans ; Infant ; Male ; Mycobacterium bovis ; Severe Combined Immunodeficiency ; complications ; Tuberculosis ; complications

Objective To evaluated a new automate ultra sonographic system for detecting benign and malignant breast tumors in three-dimensions.Methods A magnetic sensor to track the probe in a real time during scanning and a series of sequential cross-sectional images,together with information giving precise location and the direction of movement of the probe,were fed into the computer.Fuzzy logic was used to classify tumor, boundary, and normal breast tissue in order to extract an image of the tumor and its margin through three-dimensional (3D) transformation. Results The difference between the surface roughness of benign and malignant tumors was found by comparing their computer -rendered surface images.The values of the S/V ratio of surface area (S 3) divided by volume (V 2) of malignant tumors were larger than those of benign tumors. Mean ratio was 3.43 in benign tumors, 9.08 in malignant tumors.T-test results showed these differences between benign and malignant tumors to be significant(P

Objective To investigate the incidence of candidal colonization and to analyze its risk factors in patients with connective tissue diseases (CTDs). Methods Throat swabs, midstream urine and anal swabs from 153 patients with CTDs and 63 healthy volunteers were collected for fungus culture. The logistic regression was completed via the software package SPSS 11.0. Results The incidence rate of candidal colonization was higher in the patients than that in the healthy controls (35.29% vs 7.59%). Of all the isolated fungus strains, the proportion of Candida albicans was the highest. The logistic regression analysis revealed that decrease of blood erythrocytes, increase of urine protein, decrease of complements such as CH50, C3 and C4 in serum, high daily dosage of corticosteroids and broad-spectrum antibiotic administration all significantly increased the risk of candidal colonization (all P 0.05). Conclusions The incidence rate of candidal colonization in patients with CTDs is higher than that in healthy controls. Avoiding the risk factors will help to decrease the incidence of candidal colonization in CTDs.