Aim To investigate the effect of pentoxifylline(PTX) on rat mesangial cells(MCs) proliferation and the expression of connective tissue growth factor(CTGF) protein cultured in high glucose concentration. Methods The MCs were divided into 4 groups: ①normal glucose group(NG,5 mmol?L -1 D glucose),②high glucose group(HG, 30 mmol?L -1 D glucose),③HG+0 36 mmol?L -1 PTX,④HG+1 08 mmol?L -1 PTX. 72 hours later, the proliferation activity of mesangial cells was observed by MTT assay, the expression of CTGF protein was detected by Western blot. Results Compared with normal glucose group, high glucose significantly increased MCs proliferation activity and the expression of CTGF. PTX of different concentration suppressed the proliferation of the MCs and down regulated the expression of CTGF. Conclusion PTX can suppress the proliferation activity of MCs cultured in high glucose and decrease the expression of CTGF, which implies PTX probably decreases the accumulation of extracellular matrix(ECM) and slows the progression of glomerular hypertrophy and glomerulosclerosis in this way. Thus it demonstrates the protective effect of PTX in diabetic nephropathy(DN) from a new angle.

OBJECTIVETo establish human heart valve interstitial cells calcification culture model in vitro, and observe the effect of bone morphogenetic protein-2 (BMP-2) on calcification of human heart valve interstitial cells.

METHODSHuman heart valve interstitial cells were cultured in vitro, and divided into control group: cells were cultured in conventional media plus recombinant human BMP-2 treatment and experimental group: besides above treaments, calcification inducers ( recombinant human BMP-2, β-glycerophosphate, L-ascorbic acid, dexamethasone) were added to the culture media. The two group of cells were cultured for 14 days and were stained by Von Kossa, then the cell calcification was observed in this valvular interstitial cells calcification culture model in vitro. Protein expression of intercellular adhesion molecule 1 (ICAM-1), interleukin 8, BMP-2 and BMP-4 was determined by Western blot and BMP-2 secretion was measured by ELISA.

RESULTSIn the control group, the structure of human heart valve interstitial cells was clear, and the spindle and radial growth shaped cellular morphology was visible, and Von Kossa staining was negative. In the experimental group, the nuclei become darker in color, and granular sediment distribution was seen surrounding cells, and Von Kossa staining was positive, the cells were forming nodules of calcification. The protein expression of ICAM-1, interleukin 8, BMP-2 and BMP-4 in the experimental was significantly higher than that of the control group (all P < 0.05). The expression of BMP-2 in the experimental group was also significantly higher than that in control group ((92.5 ± 4.9) pg/ml vs. (22.2 ± 1.9) pg/ml, P < 0.05).

CONCLUSIONHuman BMP-2, β-glycerophosphate, L-ascorbic acid, and dexamethasone can induce human heart valve interstitial cells calcification and enhance inflammation in vitro by stimulating the secretion of BMP-2.

Objective Toinvestigatetheclinicalfeaturesandsurgicalprognosisofmoyamoya syndromeinchildren.Methods Theclinicaldataof12childrenwithmoyamoyasyndromeadmittedto the 307th Hospital of People′s Liberation Army from December 2002 to October 2013 were analyzed retrospectively. Eleven of them underwent encephalo-duro-arterio-synangiosis (EDAS). A total of 550 children with moyamoya disease in the same period were used as a control group. The clinical characteristics and surgical efficacy of the children with moyamoya syndrome were summarized and concluded by comparing the clinical data of the two groups,including sex,age of onset,initial symptom,progress symptoms, Suzukiinstallments,imagingfeatures,andsurgicalefficacy.Results Themaleandfemaleratioof the children with moyamoya syndrome was 1∶2. Their mean age of onset was 12 ± 5 years old. There were significant differences in the initial symptom (cerebral infarction and cerebral hemorrhage )and disease progress between the children with moyamoya syndrome group and the control group (5/12 vs. 14. 5%[80/550], 3/12 vs. 61. 8%[340/550],and 5/12 vs. 8.7%[48/550],respectively;all P<0. 05). Within the follow-up period,of the 11 children underwent EDAS,7 cases had no further attack,and 4 cases were improved significantly. There was significant difference in the modified Rankin scale (mRS)between the beforeandaftersurgery(0[0,1]vs.2[1,2];P<0.05).Conclusions Theclinicalfeaturesofthe children with moyamoya syndrome have some differences with those with moyamoya disease. Timely and effective EDAS treatment may effectively prevent disease progression and improve the prognosis of patients.

Objective To investigate pathogenesis of liprd metabolism disorder in children with nephrotic syndrome. Methods Serum lipid and plasma llpoprotein lipase and hepatic lipase were detected in 62 nephrotic syndrome children and 30 normal children, respectively. Results The activity of lipoprotein lipase and hepatic lipase was lower than that in normal control group, while serum cholesterol, triglycerides and low -density lipoprotein in nephrotic group were higher than those in control group. Lipoprotein lipase and hepatic lipase were negative correlation with triglycerides and low - density lipoprotein, respectively. Conclusions Reduced activity of lipoprotein lipase and hepatic lipase is one of causes leading to hypertriglyceridemia in nephrotic syndrome.

Iretreatment with tea polyphenol (TP) at a dose of 10mg/kg ip to rats five days before isoproterenol (ISO) challenge (1mg/kg sc, for two days), resulted in decreases of malonydialdehyde concentration, creatine phosphokinase, lactic dehydrogenase (LDH) and LDH1,activities; increased LDH2/LDH1 ratio and inhibited the extent of myocardial injury, similar to the action of propranol. At the same time TP decreased rat plasma renin activity.The results suggested that the mechanism by which TP protects heart from ISO-induced myocardial injury is due to its antioxygen free radical and inhibition of renin activities.

Objective To discuss the critical effect of studying and producing the practical cleaning stomach machine of once for all. Methods The process of the cleaning stomach by using the center loaded pressure system equipment was imitated, and the time of the liquid inside and outside, the whole cleaning time and the problems appeared during the cleaning stomach process was recorded. Results After finishing designing the equipment,the mimic stomach was used for critical experiment, all kinds of connecting tubes were airtight and no leaking air and water phenomena, the cleaning process was successful and it could save time to achieve the designing goal.Conclusion The cleaning stomach machine of once for all produced by using the center loaded pressure system had well solved the tube sterilized problems appeared by cleaning machine on hand, and could reduce the risk of hospital inflecting, the cleaning stomach effect was comparatively good,it had practical value and deserve to critical expanding.

An analysis of the readmission service in rural townships and counties.Based on the concept of health integration,the authors proposed the service integration among hierarchical institutions in alignment with hierarchical medical system,and the autonomous service integration among differentiated hierarchical institutions.The purpose is elevation of quality of care in rural areas and better capital usage efficiency in NRCMS.

Objectives:To test the effect of continuous care on rural hypertension control , we developed a com-prehensive intervention strategy and implemented a community-based quasi-experiment in Southwestern rural China.Methods:The intervention took place in Qianjiang District , Chongqing Municipality from July 2012 to June 2014.4 towns were randomly selected and categorized into 2 groups based on a comprehensive consideration of population and social development level.All the rural hypertension patients in treatment group were intervened with the Multi-institu-tional Continuous Pathway which was consisted of three parts:the Continuous Primary-Care Pathway , the Continuous Clinical Pathway and the Continuous Management Pathway.The patients in the control group served as a blank com-parison using the usual care.Difference-in-differences Model was used to test the effect of blood pressure control in treatment group.Results:853 patients were sampled and investigated using the stratified randomly sampling strategy and 712 of them had been followed up by the end of this program.The potential bias of pilot and patient selection were eliminated through control before and after the intervention.The previous blood pressure trends showed no statis-tically significant difference between groups.The systolic blood pressure in treatment group declined by 10.156 mm-Hg ( P<0.001 ) compared to control group after intervention , and the blood pressure control rate had increased by 27.6% ( P<0.001 ).Other contributing factors besides intervention were family structure , education level and med-ical service availability.Conclusion:The continuous care pathway have a significant marginal positive effect on hyper -tension control besides the national compulsory primary care , and the control rate change of blood pressure is more sen-sitive compared to blood pressure change.The potential contributing factors show that other intervention strategies could be developed to improve the rural hypertension control by adding to the social capital of rural patient , reinforcing the health education and facilitating the village transportation.

Objective To explore association between the changes of myenteric nerve plexus and the delayed gastrointestinal transit in acute necrotic pancreatitis (ANP) rat complicated with hyperlipemia. Methods Atotal of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups (normal control group, ANP group, hyperlipemia (HL) control group and HL with ANP group). HL rat model was established by fed with high-fat diet for 4 weeks, and ANP rat model was induced by retrograde injection of 3.5% sodium taurocholate into pancreatic duct. Gastrointestina1 transit distance was measured by ink gavage method. The histological changes of cholinergic and nitriergic nerves in myenteric plexus were observed by Karnovsky-Root method and NADPH histochemistry method. Results Pathological injuries were more severe in HL with ANP group than in ANP group (12.8±0.63 vs. 10.8±1.93,P<0.01), gastrointestinal transit was obviously delayed (transmission rate was 27%±5% vs. 38%±6%,P<0.01), the density of cholinergic neurons decreased (4.80±1.23 vs. 5.80±0.79, P<0.05), and the density of nitriergic neurons significantly increased (8.70±0.75 vs. 6.80±1.48, P<0.01). There was a linear regression between changes of cholinergic and nitrievgic nerves in myenteric nerve plexus and gastrointestinal transit (R2=0.531, P<0.01). Conclusion There was significant gastrointestinal motility disorder in the ANP rat complicated with hyperlipemia, which may be closely related with the changes of myenteric nerve plexus.

Animals ; Humans ; Occupational Exposure ; Organometallic Compounds ; poisoning ; toxicity ; Rats ; Toxicity Tests