OBJECTIVE To prevent nosocomial infection by strengthening the nursing administration.METHODS An effective system to prevent nosocomial infection,which included the further education for nurses on nosocomial infection and a normalized operation sequence,was established.The system was made to be well carried out.RESULTS The system worked normally and effectively.As a result,nosocomial infection could be well prevented.CONCLUSIONS Normalizing nursing administration is an effective method to prevent nosocomial infection.

OBJECTIVE To analyze the nosocomial infection cases,which were failed to be reported,and to grope for countermeasure to lessen the rate of nosocomial infection cases in disguise.METHODS The clinical data of the inpatients who were getting treatments in Oct 2004 and in Oct 2005 in the hospital were reviewed and analyzed.RESULTS The understanding and the recognition of nosocomial infection,the standards for judgement,the quatification of the medical workers,the medical cost and economic benefit ratio were all the causes that prevented nosocomial infection cases from being reported.CONCLUSIONS Propagandize the nosocomial infection management to make our medical workers attach importance to it,implement an objective obligation,constitute a hortative system,to exert the director's function and the workers′ enthusiasm,and choose high qualified workers to take charge in the nosocomial infection management,thus can prevent nosocomial cases from being reported.

Objective To explore the clinical features and treatment of polycythemia vera(PV)complicating stroke.Methods Clinical data of 27 cases of PV complicating stroke were analysed retrospectively.Results(1)Among all the cases,PV complicating cerebral infarction were 22 cases(81.5%),among the 22 cases,18 cases(66.7%)of multiple cerebral infarction,2 cases(7.4%)of transient ischemic attack,2 cases(7.4%)of cerebral hemorrhage,1 case(3.7%)of subarachnoid hemorrhage(SAH).The patients's clinical manifestation,physical sign,hemogram,marrow characteristic were consistent with that of PV diagnosis criteria.(2)Imageology check show that small infarction focus were common(66.7%).Lobe of brain,basal ganglia,capsula interna were common place of cerebral infarction.The density of hemorrhagic focus was asymmetrical,there was obvious edema around the hemorrhagic focus.(3)Applied vein blood-letting and small dose chemotherapy,8 cases were recovery,18 cases were obviously improved,1 case of stroke was invalid.Conclusion The cerebral infarction are common in PV complicating stroke.Among all the PV complicating stroke cases,multiple cerebral infarction are the most common,cerebral hemorrhage and subarachnoid hemorrhage are few,vein blood-letting and small dose chemotherapy has a good curative effect for the patients.

OBJECTIVE To investigate the cause,diagnosis and treatment of the empyema of the anastomotic fistula after esophageal cancer resection.METHODS The clinical data of 624 cases after esophageal cancer and cardia cancer resection from Jan 2000 to Dec 2006 were analyzed retrospectively which included 14 cases of the empyema of the anastomotic fistula.RESULTS All the cases were treated with the closed thoracic drainage,in which 9 cases were cured with closing double-tube chest douching technique,3 cases were succeeded to operate secondary thoracotomy for anastomosis,1 case died after being sent to the higher grade hospital,1 case died for toxic shock and respiratory failure without the chance of reoperation.CONCLUSIONS The key measures of prevention of empyema of post-operative esophagogastrostomy anastomotic fistula inside thorax are strictly distincting between indication and contraindication,carefully preparating before operation,commanding operative time and technical skill,strengthening nursing management,and fulfilling the oral manipulation and nursing prcedures about the drainage tube after operation.

Objective: To prepare the conjugate of supcrantigen (SAg) staphylococcal enterotoxin A (SEA) and monoclonal antibody (McAb) against human hepatocellular carcinoma HAbl8 F(ab' )_(2) fragment and to investigate the anti-human hepatoma effect of peripheral blood mononuclear cells (PBMC) targeted by HAbl8 F(ab' )i-SEA. Methods: McAb HAbl8 was extracted and its F(ab' )_(2) fragment was prepared with papain; the conjugate HAblS F(ab' )_(2)-SEA was prepared with N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP); eveny collected peak after purification was identified with gel chromatography; the activity of antibody in the conjugate was identified with immunohistocheinical ABC method; the anti-hepatoma effect of PBMC targeted by HAbl8 F(ab' )_(2)-SEA was observed with MTT method. Results: The conjugate HAbl8 F(ab' )_(2)-SEA was prepared successfully and it had obvious effect of targeting PBMC to kill hepatoma cells, and this effect is correlated positively with the dose of HAbl8 F(ab')_(2)-SEA. Control groups had no such effect. Conclusion: Targeting therapy of hepatoma with McAb-SAg conjugate might be a kind of hopeful model of hepatoma im-munotherapy.

Objective To establish the qualitative and quantitative detective methods of Fuyang granules.Methods The TLC methods for identification of Fructus kochiae and Herba solani lyrati were established.A simple HPLC was established for the determination of hydroxysafflor A(HYSA).The mobile phase was methanol-acetonitrile-0.7% phosphoric acid(24:6:70).UV detecting wavelength was at 403 nm.Results Fructus kochiae and Herba solani lyrati could be identified by TLC.HYSA showed a linear relationship at the concentration range of 0.128～1.024 ?g,r =0.999 8.The average recovery was 99.52% and RSD= 1.97%(n = 5).Conclusion The method can be used for qualitative identification and quantization determination of Fuyang granules.

Objective To investigate the status of safe injection practice in primary medical institutions.Methods 55 primary medical institutions in Hubei Province were randomly selected,questionnaire survey on status of safe injection in primary medical institutions was conducted.Results A total of 55 questionnaires were distributed,50(90.91％) valid questionnaires were obtained.All valid questionnaires were from primary level medical institutions of Hubei Province,16 of which were from community health centers,17 from township health centers,and 17 from village health clinics.None of 50 primary medical institutions repeatedly used syringe and hemostix,all used disposable sterile syringe,only 7(14.00％) medical institutions used retraction type self-destructing syringe.Only 5 (10.00％) medical institutions were equipped with hand hygiene facilities and sharps containers,32 (64.00％) medical institutions didn't provide sharps containers on treatment carts,21 (42.00 ％) injection rooms didn't install hand-drying facilities,18 (36.00 ％)medical institutions didn't provide alcohol-based hand rub on treatment carts and didn't install hand-drying facilities in treatment rooms.Of sharp injures among health care workers (HCWs) reported in 2015,114 HCWs sustained 117 times of injuries,74 cases(63.25 ％) were caused by ampoule.Of medical waste disposal,there existed some problems,such as leakage and repeated use of sharps box,didn't close sharps box timely,as well as without providing sharp containers in locations where sharps might be produced.Conclusion There are a variety of hidden risks of unsafe injection in primary medical institutions,like insufficient facilities and unsafe disposal of medical waste.

Hemophagocytic syndrome is a group of fatal immune function disorder,which can be divided into primary and secondary HPS.The disease is complex,lacking of specificity,difficult in diagnosis with quick progress,high mortality and poor prognosis.The diagnosis is mainly based on the HPS-2004 diagnostic criteria,etoposide,dexamethasone and cyclosporine would be first chosen for treatment.This article reviews the clinical advances on etiology,diagnosis,treatment and prognosis of hemophagocytic syndrome.

Objective To evaluate the diagnosis and treatment of primary breast lymphoma(PBL).Methods Clinical data of 49 PBL cases diagnosed in our center from 1997 to 2011 was retrospectively analyzed.Results 1 case was male,48 cases were female.Most patients were at the age of 35-55 years.Painless mass was the most common symptom which was most often seen on the upper-outer quadrant of the right breast,accounting for 61.11％ in this group.Preoperative misdiagnosis rate was high by mammography,B ultrasonic and hollow needle pathologic examination.Final diagnosis could only made by postoperative histopathologic examination.In this group 1 case was Hodgkin lymphoma and 48 cases were non-Hodgkin lymphoma(NHL) originating from B cells.46 patients underwent surgery and chemotherapy followed by radiotherapy in 16 cases.Median time of follow up was 40 months,5-year overall and disease free survival was 48％ and 28％ respectively,and bone marrow was the most common metastatic organ.Conclusions The prognosis of PBL is poor.The image diagnosis lacks specificity.Paraffin sections and immunohistochemistry were the main means to make a definite diagnosis.Chemotherapy-based comprehensive treatment is the strategy in the management of PBL instead of radical mastectomy.

Objective To study Heat shock proteins (HSPs) induction and their association with Hantaan virus (HTNV) structural proteins in the brain tissues of mice experimentally infected with HTNV 76～118. Methods Newborn mice less than 72 h of age were experimentally infected with HTNV by intracerebral inoculation. The brains of mice at the 8th day post infection were removed and prepared for tissue extracts. The possible interaction or association of Hantaan virus nucleocapsid protein (HTNV NP) and glycoprotein G2 (HTNV G2) with GRP94, HSP70 and HSP27 were analyzed by double specific antibo dies sandwich ELISA and immunoprecipitation methods. Results HTNV infection induced the expression a set of HSPs including GRP94 and HSP70 in the brain tissues of mice experimentally infected with HTNV. HTNV NP was associated with GRP94, HSP70 and HSP27, which led to formation of HTNV NP HSP70 GRP94 HSP27 complexes. It was also observed that HTNV G2 was associated with NP and HSP27, which led to formation of HTNV G2 NP HSP27 complexes. Conclusions HTNV infection induces expression of a set of HSPs, which were associate with viral structural proteins in the brain tissues of mice experimentally infected with HTNV. This association may demonstrate the chaperone roles of HSPs in the synthesis, transportation and maturity of viral proteins during viral replication in the host cells.