Autism in children is a complex disease of developmental disorder in nervous system,characterized by social deficits,impaired language and communication,stereotyped and repetitive behaviors.Studies indicate oxidative stress is associated with autism.Because of genic and environmental factors,children with autism are susceptible to oxidative stress.Oxidative stress induce the development of autism through abnormal metabolism of the membrane lipid,inflammation and excitotoxicity,autoimmunity,energy metabolism disorder,et al.Anti-oxidation might be a safe and effective therapy for autism.

Objective To compare and analyze two methods for determination of vaccine particle content in freeze-dried human rabies vaccine(Vero cells)bulk and final bulk,and to provide experimental basis for establishing standard detection method for particle content and purity of rabies vaccine products.Methods The samples were subjected to vaccine particle cracking,BCA quantification,and size exclusion chromatograghy-high performance liquid chromatography(SEC-HPLC)analysis. BCA-SEC relative area method and BCA-SEC vaccine particle standard curve method were used to detect the total protein concentration of the bulk and final bulk,separately,and the content of vaccine particles was quantitatively analy-zed. Finally,the detection results of the two methods were compared and analyzed.Results The content of vaccine particles in freeze-dried human rabies vaccine bulk was determined to be within 256-305 μg/mL by the two methods,with an average value of 267-285 μg/mL,and the relative standard deviations(RSDs)ranged from 5. 8% to 10. 2%,with good consistency between two methods. The content of vaccine particles in freeze-dried human rabies vaccine final bulk was determined to be in the range of 149-169 μg/mL by the two methods,with an average value of 152-164 μg/mL,and the two methods showed the RSDs between 0. 9%-4. 7% with good consistency.Conclusion The measured value by BCA-SEC standard curve method deviates less from the expected value and is closer to the actual situation of samples,so it is recommended to use this method as a reference for enterprises.

Objective:The aim of quality control in the key process of clinical laboratory is to provide accurate and reliable and timely clinical laboratory test reports. Methods:Strictly enforce the national development ofmedical institution clinical laboratory management approach, and to quality control from the several important aspects, such as the basic information before clinical examination, clinical laboratory equipment, reagents management, software conditions, issue of test reports, which should ensure quality control throughout the entire process of testing. Results:Thought strengthening of clinical laboratory quality control can greatly improve the accuracy of test results, reduce the test results influence because of subjective and objective factors in the testing process, which will promote and improve the quality of clinical testing. Conclusions:It is important to the quality of clinical laboratory tests and hospital medical quality and safety. Thought strengthening quality control in the key process of clinical laboratory, accurate test data can be provide for clinical diagnosis and treatment, and then effectively improve the level of the hospital.

?AIM: To investigate the corneal high order aberrations in adults with myopia and to study its correlations with myopic degree and keratometry.?METHODS: A total of 63 patients ( 126 eyes ) from the ophthalmology department of our hospital were included in this study during May 2014 to June 2015. Visual acuity, intraocular pressure ( IOP ) , diopter, slit lamp examination, central corneal thickness ( CCT ) , funduscopic examination, corneal topography and corneal total higher order aberrations ( THOAs ) were recorded. Right eye was selected. According to the spherical equivalence ( SE) , 63 eyes were divided into two groups, the mild to moderate myopia group (M-M group, SE<-6. 00D) and the high myopia group (H group, SE≥-6. 00D). The correlations of corneal THOAs with diopter, keratometry, IOP and CCT were analyzed. Kolmogorov-Smirnov Test was used for normality test. Independent-samples t test was used for comparison between two groups. Pearson correlation analysis was for comparison between two continuous variables.?RESULTS: Z44 was found a statistic difference between the M-M group and H group (t=2. 51,P=0. 02). IOP was negatively correlated with Z5-3(r= -0. 52,P=0. 01), and CCT was positively correlated with Z4-4(r=0. 43,P=0. 03) in H group. There was no correlation between higher order aberrations and SE in M-M group ( all P>0. 05 ) Total coma aberration ( TCA ) was negatively correlated with SE (r=-0. 57,P=0. 002) and spherical degree (Sph, r=-0. 55,P=0. 003) in H group. Z40 was positively correlated with average K (AK) (M-M group:r=0. 40,P=0. 02. H group:r=0. 55, P=0. 03), K steep (Ks) (M-M group:r=0.39, P=0.02. H group:r=0.51, P=0.01) and K flat (Kf) ( M-M group:r=0. 40, P=0. 02. H group:r=0. 57, P=0. 01) in both groups. Z53was negatively correlated with Ks(r=-0.4, P=0. 04) in H group.?CONCLUSION: The THOAs parameters are influenced by diopter and keratometry. TCA is negatively correlated with SE and Sph in patients with high myopia, and spherical aberration is positively correlated with keratometry.

Objective To explore diagnostic value of the immune intensity of platelet ?-amyloid precursor protein(APP) and isoforms proportion for Alzheimer's disease(AD).Methods The APP immune intensity of platelets was evaluated by flow cytometric detection and the isoforms proportion of APP130/APP106 was also detected by Western Blot analysis in 31 AD cases, 22 vascular dementia (VD) patients , 28 neural degeneration patients and 30 healthy elders.Results There was no significant difference of APP immunity in the each group. The ratio of APP130/APP106 was significantly lower in the AD group compared with the groups VD, degenerative disorder and healthy elders(all P

Objective:To explore the mediating effect of attitudes going into the of undergraduates world or leaving the mundane world between internet addiction disorder (IAD) and undergraduates' well-being. Methods:603 undergraduate students were surveyed with the Scale of Going into the World and Leaving the World, the Scale for Internet Addiction and the Multiple Happiness Questionnaire. Correlation, regressions and structural equation were adopted. Results:The rate of IAD in our sample was 9.1%, which was higher in male (47persons, 13.2%) than in female (8persons, 3.2%)(?2=16.47,P

Objective To investigate the distribution of intramuscular nerves in the different skeletal muscles of the rabbit. Methods Muscle architecture and modified sihler's neural staining methods were used. Results The nerve branches of innervating flat M.pectoralis major arose from the anterior pectoral and posterior pectoral nerve.The former innervated cross fibers mainly,it penetrated the middle of muscle belly and formed a “U-Shaped” nerve ansa.The latter innervated oblique fibers mainly.There were many anastomoses between them.The nerve of pennate M.plantaris derived from tibial nerve.After entering muscle,the nerve trunk gradually divided into many primary branches toward medial and lateral fibers,these branches then subdivided into numerous arboroid second and terminal branches toward all of muscle fibres;The nerve of innervating spindle M.extensor digitorum longus came from N.fibularis communis.Two extramuscular nerve trunk were seen.Superior trunk mainly innervated those muscle fibers of inserting in the second toe,Inferior one mainly distributed to the fibers of rest within this muscle.Conclusion The spatial arrangement of the muscle fibres were related to distribution of the intramuscular nerves;Walking of intramuscular nerve branches had two patterns which run perpendicular and/or parallel to muscle fasciculi.

Objective To compare the effects of bifidobiogen with enzyme inductor(luminal, nikethamidum) in treatment early neonatal hyperbilirubinemia. Methods 92 babies with early neonatal hyperbilirubinemia were divided into 2 groups, randomly. 49 patients were treated with bifidobiogen 350 mg/(kg. d) and phototherapy. Other 43 patients were treated with enzyme lnductor luminal 5mg/(kg. d) ; nikethamidum 100mg/(kg. d)and phototherapy as the control group. Results The total effective rate in the treatment gr0up(77. 6% ) was higher than that in the control group (44. 2% ), P

OBJECTIVE:To study the contents of the medicinal components in Artemisia annua from different habitats.METHODS:A total of 22 batches of Artemisia annua samples from different habitats throughout China were collected with artemisinic acid,arteannuin B,artemisinin and scopoletin determined by HPLC.The contents of the constituents were taken as indexes to conduct correlation analysis and cluster analysis.RESULTS:The results of correlation analysis and cluster analysis showed that there were marked differences in the contents of the above-mentioned 4 constituents in Artemisia annua from various habitats.CONCLUSION:Accumulation of active ingredients of Artemisia annua is correlated to the habitats to some degree.

After the research of PLA2R1 and its antibodies, Thrombospondin type-1 domain-containing 7A and its antibodies to membranous nephropathy (MN) has made a new understanding.Some researches have reported that the antibodies of PLA2R1 and THSD7A were mutually exclusive in MN, because THSD7A was found in PLA2R1-negative MN patients.But the latest researcher showed that these antibodies can be both positive in MN patients.Similar to the function of PLA2R1, THSD7A can assist clinical diagnosis, treatment, and monitor of MN.In contrast to PLA2R1, THSD7A was also highly expressed on both human and murine podocytes.We can use the mice model to study the pathogenesis of THSD7A-associated MN in the future.In this review, we describe the structure and function of Thrombospondin type-1 domain-containing 7A and its autoantibodies, highlight its role in MN and suggest possible aspects of its future clinical application.