Objective:To investigate the feedback regulation of transforming growth factor ?(TGF-?)to tumor necrosis factor-? converting enzyme.Methods:Reverse transcription polymerase chain reaction(RT-PCR)and immunochemistry were applied to detect TACE mRNA and protein in mice endometria of experiment group(TGF-? antibody was injected)and control group(saline was injected).Results:The expressions of TACE mRNA and protein in control group were higher than in experiment group.Conclusion: TGF-? could feedback on TACE expression in mice endometria and TACE-TGF-?-EGFR might one of the regulations during blastocyst implantation.

Ultrasound,MRI and mammography play significant roles in diagnosis,staging and follow-up of patients with breast cancer.With the development of individualized treatment of breast cancer,the molecular classification of breast cancer has vital reference value for treatment protocols.The requirements of medical imaging evolve from detecting breast cancer by morphological characteristics to making more accurate diagnosis using functional imaging for breast cancer.Correlations between molecular subtypes and ultrasonic,mammographic and MRI features of breast cancer attract broad attention.The correlation of molecular subtypes with uhrasound,MRI and mammography features in breast cancer patients were reviewed in this article.

Objective To establish a pre-column derivatization-high performance liquid chromatography(HPLC) method for the determination of free formaldehyde in Sabin strain inactivated poliovirus vaccine(IPV)(Vero cells), validate and apply the method, so as to provide a new method for the determination of free formaldehyde in vaccines.Methods The sample was derivatized with 2, 4-dinitrophenylhydrazine and loaded onto a C18 chromatographic column(5 μm, 120 ?, 4. 6 mm ×250 mm). The detection wavelength, mobile phase ratio, flow rate, derivatization time, temperature, buffer solution, and derivatization container were optimized for the separation conditions. The specificity, linearity, repeatability, accuracy, limit of detection(LOD), limit of quantitation(LOQ) and robustness of the method were verified. The content of free formaldehyde in 20 batches of IPV(Vero cells) was detected by using the optimized method.Results Chromatographic conditions: acetonitrile and water in a 70∶30 volume ratio as mobile phase, flow rate 0. 6 mL/min, determination at a wavelength of 352 nm.Derivatization conditions: 0. 5 mL of acetonitrile solution containing 2, 4-dinitrophenylhydrazine and 0. 25 mL of pH 5. 0buffer were added, followed by a 20 min incubation in 60 ℃ water bath. This chromatographic separation conditions effectively separated 2, 4-dinitrophenylhydrazine and formaldehyde derivatives, and the acetaldehyde had no effect on the determination results. In the range of 0. 05-100 μg/mL, formaldehyde standard concentration exhibited a good linear relationship with the peak area, with the r value of 0. 999 9. The relative standard deviations(RSDs) of six test results in the repeatability test was 0. 36%. The recovery rates of formaldehyde content in nine samples were between 102. 0% and 107. 0%. The LOD and LOQ were 0. 025 and 0. 05 μg/mL, respectively. The sample remained stable for 48 h after derivation, showing good robustness. The results of the same batch of samples had good repeatability, and the formaldehyde content was between 4. 5-9. 9 μg/dose.Conclusion The established method has the advantages of wide measurement range, good linearity and high accuracy, and can accurately quantify free formaldehyde in Sabin strain IPV(Vero cells), which can be used as an auxiliary detection method for free formaldehyde content in vaccine products, and is of great significance to the lot release and quality supervision for vaccines.

Objective To establish a pre-column derivatization-high performance liquid chromatography(HPLC) method for the determination of free formaldehyde in Sabin strain inactivated poliovirus vaccine(IPV)(Vero cells), validate and apply the method, so as to provide a new method for the determination of free formaldehyde in vaccines.Methods The sample was derivatized with 2, 4-dinitrophenylhydrazine and loaded onto a C18 chromatographic column(5 μm, 120 ?, 4. 6 mm ×250 mm). The detection wavelength, mobile phase ratio, flow rate, derivatization time, temperature, buffer solution, and derivatization container were optimized for the separation conditions. The specificity, linearity, repeatability, accuracy, limit of detection(LOD), limit of quantitation(LOQ) and robustness of the method were verified. The content of free formaldehyde in 20 batches of IPV(Vero cells) was detected by using the optimized method.Results Chromatographic conditions: acetonitrile and water in a 70∶30 volume ratio as mobile phase, flow rate 0. 6 mL/min, determination at a wavelength of 352 nm.Derivatization conditions: 0. 5 mL of acetonitrile solution containing 2, 4-dinitrophenylhydrazine and 0. 25 mL of pH 5. 0buffer were added, followed by a 20 min incubation in 60 ℃ water bath. This chromatographic separation conditions effectively separated 2, 4-dinitrophenylhydrazine and formaldehyde derivatives, and the acetaldehyde had no effect on the determination results. In the range of 0. 05-100 μg/mL, formaldehyde standard concentration exhibited a good linear relationship with the peak area, with the r value of 0. 999 9. The relative standard deviations(RSDs) of six test results in the repeatability test was 0. 36%. The recovery rates of formaldehyde content in nine samples were between 102. 0% and 107. 0%. The LOD and LOQ were 0. 025 and 0. 05 μg/mL, respectively. The sample remained stable for 48 h after derivation, showing good robustness. The results of the same batch of samples had good repeatability, and the formaldehyde content was between 4. 5-9. 9 μg/dose.Conclusion The established method has the advantages of wide measurement range, good linearity and high accuracy, and can accurately quantify free formaldehyde in Sabin strain IPV(Vero cells), which can be used as an auxiliary detection method for free formaldehyde content in vaccine products, and is of great significance to the lot release and quality supervision for vaccines.

China ; Female ; Humans ; Male ; Population Surveillance ; Tobacco Use Cessation ; methods ; Tobacco Use Disorder ; epidemiology ; prevention & control ; World Health Organization

Adrenergic alpha-1 Receptor Antagonists ; therapeutic use ; Anti-Inflammatory Agents, Non-Steroidal ; therapeutic use ; Calcium Channel Blockers ; therapeutic use ; Glucocorticoids ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Urinary Calculi ; drug therapy

OBJECTIVETo investigate clinical curative effects of gluteal muscle contracture release combined with insertion of gluteus maximus tendo-chilles lengthening with Z-shaped in treating severe gluteal muscles contracture.

METHODSFrom 2006 May to 2011 May, 20 patients (35 sides) with severe gluteal muscle contracture were collected, including 12 males and 8 females, aged from 8 to 34 years old with an average of 13 years old; the courses of disease ranged from 3 to 21 years. All patients manifested abnormal gait at different degree, knees close together cannot squat,positive syndrome of Ober, positive test of alice leg. Gluteus contracture fascia release were performed firstly in operation, then insertion of tendo-chilles lengthening with Z-shaped were carried out. Preoperative and postoperative gait, and knee flexion hip extensor squat test, cross leg test, adduction and internal rotary activity of hip joint, stretch strength and motor ability after hip abduction were observed and compared.

RESULTSTwenty patients were followed up for 1 to 5 years. Gluteus maximus were released thoroughly, and snapping hip was disappeared, Ober syndrome were negative. There was significant differences in knee flexion hip extensor squat test, adduction and internal rotary activity of hip joint,stretch before and after operation (P<0.01). Gluteus muscle strength was protected,stretch strength and motor ability of hip joint were recovered well. Among them,31 cases got excellent results and 4 good.

CONCLUSIONFor severe gluteal muscles contracture,insertion of gluteus maximus tendo-chilles lengthening with Z-shaped performed after gluteus contracture fascia release could release gluteal muscle contracture to the greatest extent and obtain postoperative curative effect without resection of normal hip muscle fibers and destroy joint capsule.

Adolescent ; Adult ; Buttocks ; surgery ; Child ; Contracture ; surgery ; Female ; Hip ; surgery ; Humans ; Male ; Muscle, Skeletal ; surgery ; Thigh ; surgery ; Young Adult

Objective To analyze the MR findings of vagal paraganglioma.Methods Six cases of vagal paraganglioma(4 benign and 2 malignant)were retrospectively analyzed with the emphasis on the location,MR signal intensity,dislocation of the carotid vessels,and the metastasis of the tumor.Results The tumors located in the superior(4 cases),middle(1 case)and inferior(1 case)neck,respectively,with right-sided in 4 cases and left-sided in 2 cases.The signal intensities of the six tumors were heterogeneous,combined with 'salt and pepper' appearance in 4 cases in which the 'pepper' appearance was more marked.On MR angiography performed in 4 cases,carotid arteries were found dislocated anteriomedially.Two cases were diagnosed as malignancies by the manifestations of destruction of surrounding bone,metastasis of bilateral lungs and/or the lymph nodes.Conclusion Vagal paragangliomas could be correctly diagnosed before surgery according to their locations and signal intensities.

Objective To investigate the effect of thalidomide and As_2O_3 to the secretion of VEGF of the cell of ANLL and study if there is coorperation in thalidomide and As_2O_3.Methods From October 2001 to February 2003,23 patients with ANLL were analyzed.Heparinized marrow was taken,then it was putt into Ficoll-isopaque solution to be purified for MNC,which was cultured in the RPMI 1 640 medium,placed in 5% CO_2 saturation and 37℃ to proliferation.Choosing the cells in logarithm growth phase,adding cultural solution to abstaining the cellular density of 2?10~9/L and then putting it into 24-well plates.There are 4 groups :contrast group,thalidomide(300 ?g?mL~ -1 )group,As_2O_3(25 ?mol/L)group,and thalidomide(300 ?g?mL~ -1 )andAs_2O_3(25 ?mol/L)group,cultured for 72 hours.Examine the concentration of VEGF in upper liquid with ELISA method.Results The concentration of VEGF in upper liquid of 4 groups were respectively(1 582.67?281.51)ng/L、(1 206.29?264.87)ng/L、(941.72?236.34)ng/L、(639.12?211.98)ng/L.There were statisticd difference(P

BACKGROUND:Hepatic vascular exclusion is important for liver transplantation that can reduce blood loss and make for liver recovery. A variety of favorable hepatic vascular exclusion techniques occur in the development of liver surgery technology, which is stil a research hot in surgical study. OBJECTIVE:To introduce the latest research and the hot spot of hepatic vascular exclusion techniques. METHODS:A computer-based online search of PubMed and Wanfang databases for articles relevant to Pringle maneuve, total hepatic vascular exclusion, selective hepatic vascular exclusion and sectional vascular exclusion under hepatectomy published from January 1999 to January 2014. Totaly 50 articles were included in result analysis. RESULTS AND CONCLUSION:There are a variety of hepatic vascular exclusion technologies, and intermittent hepatic vascular occlusion and semihepatic vascular exclusion are used most commonly. The applicable principles are as folows: (1) Surgery without vascular exclusion is suitable for < 5 cm lesions at the liver edge. (2) Semi-hepatic vascular exclusion is fit for semi-hepatic lesions, especialy for patients accompanied by liver cirrhosis. Hepatic vascular exclusion with preservation of semi-hepatic artery and liver hanging maneuver are also reported to have a certain value in clinical practice stil need further studies. (3) Intermittent hepatic vascular exclusion is suitable for lesions over half a liver or spanning liver halves (huge lesions). (4) Total hepatic vascular exclusion and its modified technologies are suitable for lesions involving the inferior vena cava and (or) hepatic vein, or lesions closely related to the second and third porta hepatis. (5) Segmental hepatic vascular exclusion is considered for smaler lesions confined to the liver segment under alowed conditions, but semi-hepatic vascular exclusion and Pringle maneuver can be also considered. Depending on patient’s conditions, to select the appropriate method is the key to reduce bleeding and to ensure patient safety.