Objective: To analyze the relationship among cardiopulmonary endurance, body composition, flexibility, muscle strength and endurance with functional movement performance of male college students, and to explore the factors associated with the performance of functional movements. Methods: A total of 112 male college students were selected, muscle power and flexibility test was based on the national student physical health standard, founctional movement screen created by Cook was used to test founctional movement performance. Results: Male college students have poor flexibility, stability and control of functional movements, and high probability of compensatory or asymmetric movements; In-line lunge, trunk stability push-up were positively associated with cardiorespiratory fitness(r=0.17, 0.17, P<0.05); hurdle step, shoulder mobility, trunk stability push-up and rotary were negative associated with body composition(r=-0.17, -0.13, -0.25, -0.22,P<0.05); shoulder mobility, trunk stability push-up and rotary were positively associated with score of standing long jump, trunk stability push-up was positively associated with endurance of deltoid and trapetus as well as endurance of pectoralis major and latissimus dorsi; shoulder mobility was positively associated with score of sit and reach(r=0.17, P<0.01). The independent risk factors of functional performance of male college students, hurdle step(β=-0.02), trunk stability push-up (β=-0.12) and rotary were body fat rate(β=-0.03); the protective factors trunk stability push-up were body fat rate(β=-0.04), VO 2max (β=0.04) and endurance of pectoralis major and latissimus dorsi(β=0.03). Conclusion The functional performance of male college students was not optimistic, which could be improved by increasing health-related physical fitness.