Objective:To investigate the clinical characteristics of urinary system abnormalities in Chongqing and its surrounding area, and to assess the role of the large-scale urinary system ultrasound screening in the asymptomatic children with kidney and urinary tract abnormalities. Methods:We performed urinary system ultrasound on Screening 14 256 children from September 2008 to October 2008 in Chongqing Children’s Hospital. The total samples were allocated into five groups as follows:less than 1 year group;1 to 3 years group;3 to 5 years group;5 to 10 years group ;more than 10 years group. Then according to each group’s age,sex,disease location,severity difference,we performed statistical analysis. Results:409(2.87%)of 14 256 children had abnormalities,284 of whom was diagnosed with significant anomalies,including hydronephrosis,renal maldevepment (aplastic,dysplastic,hypoplastic,ectopic kidneys)and renal agensis,nephrolithiasis,simple renal cyst,multicystic dysplastic kidneys (MCKD),ureter dilation,duplex kidneys and so on. Among these anomalies,the most prevalent abnormal finding was hydronephrosis accounting for 130 cases,almost a half of 284 (45.77%),92 cases in males,38 cases in femal. The prevalence of melamine related urinary calculus was as 5.17 times as that of others and its 95% confidence interval(CI)of population relative risk was [3.28,8.14]. Conclusion:① Ultrasonography in the diagnosis of diseases of the urinary system can play an important role,especially for congenial anomalies of kidney and urinary tract(CAKUT)and /or hydronephrosis and so on. Therefore,an early diagnosis of these defects allows to realize as fast as possible the best medical and/or surgical treatment, preventing or at least slowing down the progression to chronic renal failure. ② The prevalence rate of hydronephrosis in male is higher than female. ③Exposure to melamine- contaminated formula is associated with urinary stones.

Objective: To study effects of tertiary rehabilitation training on upper limb motor function recovery in patients with cerebral hemorrhage.Methods: A total of 120 patients with cerebral hemorrhage, who were treated in our hospital from May 2013 to May 2015, were selected.According to random number table, they were randomly and equally divided into routine rehabilitation group and tertiary rehabilitation group, and both groups received the same routine medical treatment and nursing.Simple test for evaluating hand function scale (STEF), Fugal-Meyer motor assessment (FMA) and Brunnstrom limb motor function assessment were used to assess and compare limb recovery after rehabilitation training between two groups.Results: At the end of first month of rehabilitation training, there were no significant difference in STEF and FMA scores between two groups, P>0.05 both.On the end of second month of rehabilitation training and later, compared with routine rehabilitation group, there were significant rise in STEF score[end of second month: (3.3±0.5) scores vs.(4.2±1.1) scores, end of sixth month: (6.3±1.2) scores vs.(8.5±1.1) scores], total STEF score[(20.2±4.2) scores vs.(38.2±3.5) scores]and FMA score[end of second month: (28.4±3.6) scores vs.(33.4±2.7) scores, end of sixth month: (38.2±6.6) scores vs.(46.3±5.8) scores]in tertiary rehabilitation group, P<0.05 all.Compared with routine rehabilitation group after six-month rehabilitation training, there were significant rise in percentages of Brunnstrom stage V (23.33% vs.43.33%) and stage VI (6.67% vs.20.00%) in tertiary rehabilitation group(P<0.05 both).Conclusion: Tertiary rehabilitation training can significantly improve upper limb motor function in patients with cerebral hemorrhage, so it's worth extending.

BACKGROUND:Traditional Chinese medicine shares wide application in clinical treatment because of its high security. At present, a lot of studies have been reported, in which, traditional Chinese medicine or monomers are used for in vitro differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
OBJECTIVE:To summarize and analyze the research progress of Chinese medicine to induce the differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells and to looking prospect for its application.
METHODS:CNKI database was searched for the articles about Chinese medicine-induced differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, published from January 2001 to January 2013. The key words were“Chinese medicine, bone marrow mesenchymal stem cells, cardiomyocyte”in Chinese and“bone marrow mesenchymal stem cells, myocardial cells”in English. Obsolete or repetitive articles were excluded. Final y, 36 articles were included in result analysis.
RESULTS AND CONCLUSION:Different Chinese medicine formulations were used as inducers to induce differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, including Panax notoginseng saponins, salvianolic acid B, Jiawei Danshen Yin, icari n, astragaloside. Then, the induced cells were examined by immunohistochemistry and reverse transcription-PCR. The results showed that the induced cells were positive for Desmin, cardiac troponin I and major histocompatibility complex. Positive cells were fusiform and fibroblast-like morphology, which indicates a role in promoting proliferation and differentiation. These findings show that Chinese medicine-induced differentiation of bone marrow mesenchymal stem cells into cardiomyocytes has become a worldwide research hotspot, providing a theoretical basis for clinical treatment of ischemic heart diseases.

Objective To explore the clinical effects of injecting methotrexate with hysteroscopic tubal can-nulation combined with Huayu shapei prescription in the treatment of salpingocyesis.Methods 82 unruptured salpin-gocyesis patients were randomly divided into the two groups.41 patients in the observation group were treated by hyst-eroscopic tubal cannulation injection of methotrexate combined with Huayu shapei prescription,41 patients in the con-trol group were treated by methotrexate intramuscular injection with oral mifepristone.The clinical efficacy of the two groups were observed,the level of serumβHCG required for tubal pregnancy,the mass absorption and after treatment of tubal patency.Results Compared with the control group,the difference was significant of the decline of serum βHCG in the treatment group (t=2.67,P<0.05).Compared with the control group,the treatment effectiveness and the absorption of the mass in fallopian tube in the treatment group,the difference was significant (t =3.57,P<0.05 ).Tubal patency detected treated group was superior compared with the control group,the difference was signifi-cant(χ2 =100.23,P<0.05).Conclusion Compared with traditional treatments,injecting methotrexate with hyste-roscopic tubal cannulation combined with Huayu shapei prescription in the treatment of salpingocyesis can effectively reduce the HCG levels,promote the absorption and softening of the ectopic pregnancy mass,to achieve the purpose of killing embryos eliminate micro,it will help to improve patients with clinical symptoms and signs,to maximize the recovery of the patient's reproductive function and physical health.

Objective To investigate the involvement of apoptosis in the lesions of patients with psoriasis. Methods The apoptosis was detected with terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL), and the expression of p53, PCNA and apoptosis suppressing protein bcl 2 was assessed with immunoperoxidase technique in psoriatic lesions and normal skin. Results A large number of keratinocytes showing biochemical and morphologic features of cells undergoing apoptosis were observed in all the suprabasal layers of the psoriatic epidermis. The plaques from all patients analysed showed marked increase in the number of PCNA positive cells in the middle and basal keratinocytes, and a dramatic reduction in the number of bcl 2 positive cells in the basal cell layer. Conclusion The increased apoptosis of keratinocytes in the lesions of psoriasis might be a homeostatic mechanism to the hyperplasia of cells.

Objective: To evaluate the effect of dietary fiber and phytic acid inositol hexaphosphate (IP6 or Insp6) on 1,2-dimethylhydrazine(DMH) induced colorectal carcinogenesis in Wistar rats. Methods: 86 four-week-old male Wistar rats were divided randomly into 6 groups and fed with either basal fiber free diet or the basal diet supplemented with 10% pectin, 10% cellulose, 2% Na-InsP6 supplemented water, 10% pectin in combination with 2% Na-InsP6 supplemented water, 10% cellulose in combination with 2% Na-InsP6 supplemented water. After four weeks, the rats were given a weekly injection of DMH (20mg/kg bw) for 20 weeks. The rats were killed after 26 weeks. The number of total macroscopically visible neoplasms was counted and the volume of individual neoplasmas was calculated. Proliferation cell nuclear antigen (PCNA) was analyzed. Results: There was no significant difference between the treatment group and the control group in the incidence of tumors. InsP6 significantly reduced the number of tumors in rats and tumor volume. Pectin and Pectin+InsP6 significantly increased the number of tumors in rats. The expression of proliferation marker PCNA was significantly down-regulated by InsP6 and significantly increased by pectin. Conclusion: A treatment regimen of 2% Na-InsP6 in drinking water was effective in significantly reducing the risk of colorectal cancer in Wistar rats. Diet supplemented with pectin could increase the risk of colorectal cancer.

Objective To approach the involved position and regulation as well as the clinical characteristics of hereditary multiple exostoses(HME).Methods One HME pedigrees including men and women 7 cases respectively confirmed clinically and pathologically was analysed.Results The most common location of exostoses was metaphysis of long bones.No exostoses was found in craniofacial bone.Conclusion There is no sex difference in the incidence of HME and the location of exostoses is mostly at metaphysis of long bones in this family.

This paper analyses overall situation of the national quality inspection for medical devices in recent 13 years. The statistics cover the inspected varieties, sampling quantity and quality status. The achievements and suggestions are provided, which are helpful for future work.
Equipment and Supplies ; standards ; Humans

BACKGROUND:Bone marrow mesenchymal stem cel s have immunomodulatory properties and have potential applications in immunosuppression. OBJECTIVE:To investigate the isolation and culture of bone marrow mesenchymal stem cel s and the immunoregulation of CD8+T lymphocytes. METHODS:Primary bone marrow mesenchymal stem cel s were isolated from Wistar rats by bone marrow adherence method. The primary cel s were purified by differential adherence and digestion, and col agen type I was added as extracel ular matrix to expand bone marrow mesenchymal stem cel s. Passage 2 bone marrow mesenchymal stem cel s at densities of 0, 1×103, 1×104 and 1×105/wel were co-cultured with CD8+T lymphocytes, fol owed by phytohemagglutinin stimulation for 68 hours. T lymphocyte aggregation and proliferation were detected by staining with staining with 5,6-carboxyfluorescein diacetate succinimidyl ester and MTT, respectively. RESULTS AND CONCLUSION:Different concentrations of bone marrow mesenchymal stem cel s had an inhibitory effect on T lymphocytes, but this effect was weakest for the cel s at the density of 1×103 per wel and strongest for the cel s at the density of 1×105 per wel . Obvious agglomeration was observed in the same media. The proliferation rates of CD8+T lymphocytes were (44.83±4.92)%, (31.94±6.28)%and (15.77±3.98)%, respectively, after co-culture with 1×103, 1×104, 1×105/wel bone marrow mesenchymal stem cel s. There were significant differences between groups (P<0.05). These results showed that primary rat bone marrow mesenchymal stem cel s could be obtained by direct adherence method of the whole bone marrow, and the cel s could be purified by differential adherence combined with digestion and control method. Bone marrow mesenchymal stem cel s could inhibit T lymphocyte proliferation in a concentration-dependent manner.

Objective To probe into the optimal concentration of Wnt-11 to induce the differentiation of bone marrow mesenchymal stem cells (BMMSCs)into cardiomyocyte-like cells in vitro.Methods BMMSCs were isolated from the bone marrow of SD rats using whole bone marrow culture method.After cultured for 48 h, BMMSCs of the second generation were utilized for directed induction.Based on the final concentration of Wnt-11 , BMMSCs were divided up into Group A (100 ng/mL),Group B (200 ng/mL),Group C (400 ng/mL)and Group D (blank control).After 72-hour induction,the cells were cultured in complete medium for 4 weeks while cells in Group D were cultured only in the complete medium.The morphological changes were observed under the phase contrast microscope.Surface antigen expression of BMMSCs was identified by flow cytometry.When cells were cultured for 4 weeks,the expressions of Desmin,Connexin43 and cTnI were detected by immunocytochemistry. Meanwhile, the ultrastructural changes were observed using transmission electron microscope. The mRNA expressions of cardiac transcription factors GATA-4,Nkx2.5 andα-MHC in BMMSCs were detected by RT-qPCR at 1,2 and 4 weeks after induction.Results Primary BMMSCs formed cell colonies at 2 weeks;the cells were mainly fusiform or star-shape,and a few irregularly-shaped ones were also visible.The passaged cells were larger than those of primary culture.After induction,the cells exhibited long shuttle-shape and were aligned in parallel. Flow cytometery displayed that the positive rate of the surface antigens of BMMSCs CD29,CD45,and CD90 was 97.9%,0.4% and 99.5%,respectively.When BMMSCs-induced via Wnt-11 were cultured for 4 weeks,Desmin, cTnI and Connexin43 were all positively expressed in induction groups.Whereas in the blank control group they were slightly positive or negative;the positive rate in Group B was the highest (P<0 .05 ).Transmission electron microscopy exhibited that organelles such as rough endoplasmic reticulum,mitochondria,as well as some ribosomes were visible in the cytoplasm of these cells in each induction group.In addition,myofilaments were arranged in parallel in the cytoplasm.The cells in induction groups could express GATA-4 and Nkx2 .5 in the first week,and then the expression of them decreased in the second week,but then increased in the fourth week;gene expression in induction Group B was significantly higher than in the other two induction groups (P<0 .05 ).The expression of GATA-4 and Nkx2 .5 in Group D was 1 ,α-MHC was not expressed in the four groups during the culture period. Conclusion Wnt-11 can induce the differentiation of BMMSCs into cardiomyocyte-like cells in vitro,and the optimal concentration of Wnt-11 is 200 ng/mL.