Objective To understand the current situation and influencing factors of humanistic care ability of nurses in emergency department. Methods A total of 101 nurses in the emergency department of our hospital were selected to conduct a questionnaire survey using the Humanistic Care Ability Inventory (CAI). The influencing factors of humanistic care ability of nurses in emergency department by multiple regressions were analyzed. Results The total score of humanistic care ability of emergency department nurses was 220.50 ± 3.41, and there were significant differences between the total scores of all dimensions and foreign conventional model (t=3.07-5.98, P<0.05), indicating that nurses′humanistic care ability were at a high level. In the three dimensions, the orders of the total scores from high to low were:cognitive dimension, courage dimension, and patience dimension. There was statistically significance among the different grades, different titles, family members and colleagues with different caring ability degree (F=3.92-48.98, P<0.05). The higher of the grades, the titles, and the caring degree of the family and the colleagues, the stronger the humanistic care ability was obtained. There was no significant difference between all dimension scores and the total scores of male and female nurses (t=0.93-1.70, P > 0.05). There was no difference in the humanistic care ability of nurses with different sexes. Multivariate regression analysis showed that the grades and the care degree of colleagues was the most influential factor for influencing the humanistic care ability of nurses in emergency department. Conclusions Humanistic nursing ability of emergency department nurses was at a high level. Through strengthening of the nursing ability of the low-grade nurses, it can effectively improve the nurses' humanistic nursing care ability, and promote the successful development of the quality nursing work.

Education, Professional ; Gastroenterology ; education ; Gastrointestinal Diseases ; surgery ; Humans ; Physicians

Animals ; Disease Models, Animal ; Female ; Hemorrhoids ; etiology ; Humans ; Male ; Rabbits

Objective: To investigate the relationship of involvement area with the clinical, endoscopic and pathological characteristics of ulcerative colitis (UC). Methods: We retrospectively studied the clinical, endoscopic and pathological data of 75 patients with UC, including 17(22.67%) with distal colitis,27(36%) with left-sided colitis and 31(41.33%) with extensive colitis/pancolitis. Results: No differences were found in the gender and age of onset between different groups. The incidences of main clinical manifestations and complications increased with the increase of the UC involvement, with significant difference found in the incidences of anemia, hypoproteinemia and extraintestinal manifestations between different groups (P<0.05). Positive correlation was found between the UC involvement with the UC severity (r=0.418, P<0.001) and disease activity under endoscopy (r=0.239, P=0.039). The incidence of atypical hyperplasia of enteric epithelium in extensive colitis/pancolitis group was significantly higher than those in the other 2 groups (both P<0.05). Conclusion: The UC involvement is related to the clinical, endoscopic and pathological characteristics of UC; the involvement is positively correlated with the clinical severity and disease activity of UC under coloscopy.

Background The pathogenesis of pterygium has been in the study.Relative molecular biology study showed that pterygium is a tumor-like lesion,and based on overseas literatures,human papillomavirus (HPV) is positively expressed in 100％ patients with pterygium in some region.However,if this result is suitable for Chinese patients is unclear.Objective This study was to identify the role of human HPV in the pathogenesis of pterygia in Wuxi area.Methods Forty-eight pterygium specimens including 7 recurrent pterygia and 41 primary pterygia were collected during the operation,and these patients were from Wuxi area.Two cervical carcinoma specimens and 2 conjunctiva specimens from normal donors were obtained as positive control and negative control respectively.The fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect HPV DNA of specimens.Results The amplified curves of HPV 6,11 of pterygium specimens,cervical carcinoma specimens and normal specimens were all below the positive quality control curve,but the amplified curves of HPV16,18 were above the quality control curve in cervical carcinoma specimens; while those of pterygium specimens and normal conjunctival specimens were all below the quality control curve.HPV16/18 was identified in 2 cervical carcinoma specimens,but no HPV6/11 was detected in 2 cervical carcinoma specimens.However,HPV DNA expression in primary and recurrent pterygias were absent.Conclusions According to these results,HPV is not the primary cause for the pathogenesis of pterygium in Wuxi region.

Adult ; Butadienes ; adverse effects ; Chemical Industry ; Environmental Monitoring ; Humans ; Middle Aged ; Occupational Exposure ; adverse effects ; Occupational Health ; Physical Examination ; Young Adult

OBJECTIVETo analyze the change in drug resistance of Staphylococcus aureus (SAU) in the PLA general hospital from January 2008 to December 2012, and to provide solid evidence to support the rational use of antibiotics for clinical applications.

METHODSThe SAU strains isolated from clinical samples in the hospital were collected and subjected to the Kirby-Bauer disk diffusion test. The results were assessed based on the 2002 American National Committee for Clinical Laboratory Standards (NCCLS) guidelines.

RESULTSSAU strains were mainly isolated from sputum, urine, blood and wound excreta and distributed in penology, neurology wards, orthopedics and surgery ICU wards. Except for glycopeptide drugs, methicillin-resistant Staphylococcus aureus (MRSA) had a higher drug resistance rate than those of the other drugs and had significantly more resistance than methicillin-sensitive Staphylococcus aureus (MSSA) (P < 0.05). In the dynamic observation of drug resistance, we discovered a gradual increase in drug resistance to fourteen test drugs during the last five years.

CONCLUSIONDrug resistance rate of SAU stayed at a higher level over the last five years; moreover, the detection ratio of MRSA keeps rising year by year. It is crucial for physicians to use antibiotics rationally and monitor the change in drug resistance in a dynamic way.

Anti-Bacterial Agents ; pharmacology ; Drug Resistance, Multiple, Bacterial ; Humans ; Methicillin-Resistant Staphylococcus aureus ; drug effects ; Staphylococcal Infections ; drug therapy ; Staphylococcus aureus ; drug effects

Background There still is no a uniform diagnostic criteria for increasing population of dry eye.Studies showed that high tear osmotic pressure is one of causes of a variety of ocular surface discomfort,and the change of tear osmolality has been included in the definition of dry eye syndrome by Dry Eye World Study Group (DEWS) in 2007.It is very clinically significant to study the relationship of tear osmolality with dry eye.Objective This clinical trial was to study the correlation between tear osmolarity and dry eye severity based on Preferred Practice Pattern(PPP)of DEWS.Methods A descriptive study was performed.Fifty-four eyes of 54 patients with dry eye disease were asked to complete an evaluation of dry eye signs and symptoms questionnaire based on PPP.The ocular anterior segment was examined under the slit lamp biomicroscope.Cornealfluorescein staining,tear-film breakup time (BUT),Schirmer test Ⅰ (S Ⅰ t)without anesthesia were performed,and tear sample was collected for tear imageology and tear osmolarity admeasurement.The correlation between dry eye score with tear osmolarity was evaluated using Pearson linear correlation analysis.Written informed consent as obtained prior to any relevant medical procedure.Results The ratio of male and female patients was 1: 2 in this study.The negative correlations were found between tear osmolarity with S Ⅰ t or BUT(r =-0.456,-0.699,P＜0.01),and significantly positive correlations were found between tear osmolarity with tear imaging examination,corneal fluorescein staining,conjunctival hyperemia or dry eye symptom score(r=0.545,0.686,0.691,0.803,P＜0.01).Conclusions As a diagnostic method,tear osmolarity can objectively reflects dry eye severity.It can be a biomarker in dry eye severity grade when combined with clinical symptom.

Objective:To construct a recombinant adenoviral vector carrying the specific siRNA of Survivin gene,and to observe its effect on the expression of Survivin gene and on the radiosensitivity of cancer cells in tumor-bearing mice.Methods:The specific siRNA of Survivin gene was designed and synthesized,and a recombinant adenovirus AdEGFP-siRNA was subsequently constructed.SMMC 7721 xenograft models were established with nude mice and were divided into the following 5 groups:siRNA+radiotherapy and siRNA groups(intratumoral injection of AdEGFP-siRNA),siRNA(-)group(injected every other day with AdEGFP-siRNA[-],2?108 pfu/100 ?l per time,total 5 times),pure radiotherapy group and blank control groups(injected with the same volume of normal saline).On day 10,12,14,and 16,the mice in siRNA+radiotherapy and pure radiotherapy groups were given 5 Gy/time radiotherapy.The tumor volumes were measured regularly.The expression of Survivin in tumor tissues was determined immunohistochemically.Results:Adenovirus AdEGFP-siRNA harboring the specific siRNA of Survivin gene and enhanced green fluorescent protein gene(EGFP)was successfully recombined.The growth of SMMC 7721 xenografts in nude mice was inhibited after injecting AdEGFP-siRNA,with the inhibition rate being 56.2%.The inhibition rate in AdEGFP-siRNA therapy + radiotherapy increased to 82.6%.Immunohistochemistry study showed that the specific siRNA markedly silenced the expression of Survivin gene in hepatocarcinoma cells.Conclusion:The specific siRNA can markedly silence Survivin gene and subsequently inhibit the growth of cancer;meanwhile,it can also increase the radiosensitivity of cancer cells so as to improve the treatment effect.