3.Construction of a recombinant vaccinia virus expressing Babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice
Nakamura, C. ; Liu, M.M. ; Goo, Y.K. ; Zhang, G.H. ; Jia, H.L. ; Kumagai, A. ; Zhang, H.S. ; Zhou, J.L. ; Nishikawa, Y. ; Xuan, X.N.
Tropical Biomedicine 2020;37(No.4):1029-1037
Previously, we have identified a gene encoding thrombospondin-related anonymous
protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against
recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the
present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was
constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of
native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/
BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native
BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing
BgTRAP might be a vaccine candidate against canine B. gibsoni infection.
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