1.National experts consensus on tracheotomy and intubation for burn patients (2018 version).
Burn and Trauma Branch of Chinese Geriatrics Society ; J MING ; P LEI ; J L DUAN ; J H TAN ; H P LOU ; D Y DI ; D Y WANG
Chinese Journal of Burns 2018;34(11):E006-E006
Airway edema, stenosis, obstruction and even asphyxia are easy to occur in patients with extensive burn, deep burn of head, face, and neck area, inhalation injuries, etc., which threaten life. Timely tracheotomy and intubation is an important treatment measure, but lack of knowledge and improper handling in some hospitals resulted in airway obstruction. The technique of percutaneous tracheotomy and intubation provides convenience for emergency treatment of critical burns and mass burn. The Chinese Geriatrics Society organized some experts in China to discuss the indications, timing, methods, extubation, and precautions of tracheotomy and intubation for burn patients. The (2018 ) .
Airway Obstruction
;
prevention & control
;
Burns
;
complications
;
therapy
;
China
;
Consensus
;
Edema
;
Emergency Treatment
;
Female
;
Humans
;
Intubation
;
Neck
;
Practice Guidelines as Topic
;
standards
;
Reference Standards
;
Tracheotomy
2.Preparation of an anti-cotinine monoclonal antibody and its application in immunological detection.
Yajing LEI ; Lifang ZHOU ; Anxing WANG ; Shuqing CHEN
Journal of Zhejiang University. Medical sciences 2020;49(6):765-771
OBJECTIVE:
To prepare monoclonal antibody against cotinine (COT) and to establish immunoassay for detecting COT in human urinary samples.
METHODS:
BALB/c mice were immunized with synthesized cotinine-bovine serum albumin (COT-BSA) to screen monoclonal antibody with technique of cell fusion. The monoclonal antibody was used for the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic strip assay for the detection of COT in human urine.
RESULTS:
The monoclonal antibody against COT was identified by ic-ELISA with a 50%inhibitive concentration (IC
CONCLUSIONS
The ic-ELISA and colloidal gold immunochromatographic strip assay using the prepared monoclonal antibody against COT have been proved to be reliable for the rapid detection of COT in human urines, which may be used for monitoring of environmental tobacco smoke.
Animals
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Antibodies, Monoclonal
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Cotinine/urine*
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Enzyme-Linked Immunosorbent Assay
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Gold Colloid
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Humans
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Mice
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Mice, Inbred BALB C
;
Urinalysis/methods*
3.Experimental study on early division of cross-finger pedicle flap and its clinical application.
Daizhong HENG ; Cong ZHANG ; Yimin YAO ; Lei LIU ; Y iping Chen YI
Chinese Journal of Traumatology 2000;3(3):159-162
OBJECTIVE: To observe the revascularization and the opportunity of cross-finger flap. METHODS: An animal model was developed to permit daily monit oring of neovascularization of the flap with autoradiography, tissue transparent technique, gross observation and histological examination. RESULTS: The revascularization of the flap was chiefly raised f rom the surrounding tissues. The pedicles of 334 cross-finger flaps of the pati ents were successfully divided from 1 to 5 d after operations, averagely 3. 3 d. All of the cases showed satisfatory results according to a follow-up sur vey of 3 to 72 mon. The results further proved that the revascularization of the cross-finger flaps had accomplished within 3 d. CONCLUSIONS: It suggests that the division of a cross-finger f lap can be carried out between the 3rd and 5th day after operation with a high d egree of safety.
4.Combined index finger pollicization with an anterolateral thigh flap for thumb reconstruction.
Guang YU ; H-Y XU ; Hong-Yu LEI ; Shuang GUO ; Jian-Hua HUANG ; Di-Bin LI
Chinese Journal of Traumatology 2014;17(6):354-357
A severe crush injury to the hand is devastating to patients. Under conditions in which the crush force is too great, the digits are not viable candidates for replantation. We present two cases in which the patients suffered from loss of the thumb ray at the first carpometacarpal joint and skin defect at the radial side. The tendons, radial nerve and metacarpal bone of the index finger were injured, and the second metacarpal head was retained. There was a comminuted fracture of the trapezium in both patients. The treatment protocols consisted of the index finger pollicization and the free anterolateral thigh flap transfer. The procedure was performed in a single operation. The new thumb is able to provide a stable post for pinch and grip after six months follow-up. Both patients were satisfied with the function and appearance of the reconstructed thumb.
Finger Injuries
;
surgery
;
Humans
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Reconstructive Surgical Procedures
;
methods
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Surgical Flaps
;
Thigh
;
Thumb
5.Development of a digital cardiac pacemaker for animals and its application in medical research.
Zhenzhong FAN ; Xiaolin YI ; Lei ZHANG ; Y P ZHANG
Journal of Biomedical Engineering 2010;27(5):1030-1033
In this research and development project, we used the general microprocessor as a core to constitute the animal cardiac pacemaker in vitro. Control of the pacemaker's settings was carried out by transmitting parameters through a serial communication interface. Finally, our pacemaker reaches a satisfying test result in activating the cardiac outer membrane of the rabbits. Full digital pacemaker has high precision, good stability, and has an intuitive way to set parameters. Owing to its smaller size, lower cost, and easier mass production, the digital pacemaker is a good candidate to replace costly medical pacemakers for activating the animal's heart.
Animals
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Electrocardiography
;
methods
;
Equipment Design
;
Male
;
Microcomputers
;
Pacemaker, Artificial
;
veterinary
;
Rabbits
;
Research
;
Signal Processing, Computer-Assisted
6.National experts consensus on tracheotomy and intubation for burn patients (2018 version).
Burn and Trauma Branch of Chinese Geriatrics Society ; J MING ; P LEI ; J L DUAN ; J H TAN ; H P LOU ; D Y DI ; Deyun WANG
Chinese Journal of Burns 2018;34(11):782-785
Airway edema, stenosis, obstruction and even asphyxia are easy to occur in patients with extensive burn, deep burn of head, face, and neck area, inhalation injuries, etc., which threaten life. Timely tracheotomy and intubation is an important treatment measure, but lack of knowledge and improper handling in some hospitals resulted in airway obstruction. The technique of percutaneous tracheotomy and intubation provides convenience for emergency treatment of critical burns and mass burn. The Burn and Trauma Branch of Chinese Geriatrics Society organized some experts in China to discuss the indications, timing, methods, extubation, and precautions of tracheotomy and intubation for burn patients. The (2018 ) .
Airway Obstruction
;
prevention & control
;
Burn Units
;
Burns
;
complications
;
therapy
;
China
;
Consensus
;
Humans
;
Intubation, Intratracheal
;
methods
;
Practice Guidelines as Topic
;
standards
;
Smoke Inhalation Injury
;
therapy
;
Tracheotomy
;
methods
7.Study on the association of oral contraceptives, angiotensin-converting enzyme gene polymorphisms and risk of stroke in women
Hui-Qiao LI ; Ing Y LI ; Chun WANG ; Tao SUN ; Zhi-Ming SUN ; Jian ZHOU ; Lei BA ; Zhi-Zheng HUANG
Chinese Journal of Epidemiology 2012;33(1):23-27
Objective To evaluate the associations of oral contraceptives (OC) exposure,angiotensin-converting enzyme(ACE) gene polymorphism and their joint actions with the risk of stroke in Chinese women.Methods A case-control study,based on a set cohort,was carried out.Incident cases of stroke identified between July 1 1997 and June 30 2009 were enrolled.One hospital control and healthy community control were matched on region and age ( ± 3 years).A total of 453women with stroke and 919 controls were recruited.I/D genepolymorphism was detected by polymerase chain reaction (PCR) and amplification fragment length polymorphism (AFLP),A-240T polymorphism were detected by TagMan.Results ( 1 ) The risk of stroke gradually increased with the cumulative time of OC being used in women (P<0.0001).Compared with non-users,the risk of stroke significantly increased among those with cumulative time of using OC longer than 20 years (adjusted OR was 2.07,with 95% CI as 1.30-3.29).(2) ID/DD genotype of I/D locus indicated significantly an increased risk of hemorrhagic stroke (adjusted OR,2.37; 95%CI,1.46-3.84).(3)Women with ID/DD genotype of I/D locus or with TA/TT genotype of A-240T locus and using OC could significantly increase the risk of hemorrhagic stroke (adjusted OR was 4.59; with 95% CI as 2.21-9.51 and OR was 2.50; with 95%CI as 1.42-4.38).(4) Data from multivariate analyses showed that both OC and ID/DD genotypes were important risk factors for hemorrhagic stroke.Conclusion ID/DD and TA/TT genotypes of ACE gene,OC and their joint action might increase the risk of stroke,especially on hemorrhagic stroke in Chinese women.
9.Chewing Lice of Swan Geese (Anser cygnoides): New Host-Parasite Associations.
Chang Yong CHOI ; John Y TAKEKAWA ; Diann J PROSSER ; Lacy M SMITH ; Craig R ELY ; Anthony D FOX ; Lei CAO ; Xin WANG ; Nyambayar BATBAYAR ; Tseveenmayadag NATSAGDORJ ; Xiangming XIAO
The Korean Journal of Parasitology 2016;54(5):685-691
Chewing lice (Phthiraptera) that parasitize the globally threatened swan goose Anser cygnoides have been long recognized since the early 19th century, but those records were probably biased towards sampling of captive or domestic geese due to the small population size and limited distribution of its wild hosts. To better understand the lice species parasitizing swan geese that are endemic to East Asia, we collected chewing lice from 14 wild geese caught at 3 lakes in northeastern Mongolia. The lice were morphologically identified as 16 Trinoton anserinum (Fabricius, 1805), 11 Ornithobius domesticus Arnold, 2005, and 1 Anaticola anseris (Linnaeus, 1758). These species are known from other geese and swans, but all of them were new to the swan goose. This result also indicates no overlap in lice species between older records and our findings from wild birds. Thus, ectoparasites collected from domestic or captive animals may provide biased information on the occurrence, prevalence, host selection, and host-ectoparasite interactions from those on wild hosts.
Animals
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Bias (Epidemiology)
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Birds
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Far East
;
Geese*
;
Lakes
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Mastication*
;
Mongolia
;
Phthiraptera*
;
Population Density
;
Prevalence
10.Chewing Lice of Swan Geese (Anser cygnoides): New Host-Parasite Associations.
Chang Yong CHOI ; John Y TAKEKAWA ; Diann J PROSSER ; Lacy M SMITH ; Craig R ELY ; Anthony D FOX ; Lei CAO ; Xin WANG ; Nyambayar BATBAYAR ; Tseveenmayadag NATSAGDORJ ; Xiangming XIAO
The Korean Journal of Parasitology 2016;54(5):685-691
Chewing lice (Phthiraptera) that parasitize the globally threatened swan goose Anser cygnoides have been long recognized since the early 19th century, but those records were probably biased towards sampling of captive or domestic geese due to the small population size and limited distribution of its wild hosts. To better understand the lice species parasitizing swan geese that are endemic to East Asia, we collected chewing lice from 14 wild geese caught at 3 lakes in northeastern Mongolia. The lice were morphologically identified as 16 Trinoton anserinum (Fabricius, 1805), 11 Ornithobius domesticus Arnold, 2005, and 1 Anaticola anseris (Linnaeus, 1758). These species are known from other geese and swans, but all of them were new to the swan goose. This result also indicates no overlap in lice species between older records and our findings from wild birds. Thus, ectoparasites collected from domestic or captive animals may provide biased information on the occurrence, prevalence, host selection, and host-ectoparasite interactions from those on wild hosts.
Animals
;
Bias (Epidemiology)
;
Birds
;
Far East
;
Geese*
;
Lakes
;
Mastication*
;
Mongolia
;
Phthiraptera*
;
Population Density
;
Prevalence