1.Comparison of Antioxidant Effect of Rehmannia glutinosa Decoction Pieces and Its Polysaccharides on Aging Model Rats before and after Processing Based on the Characteristic Ancient Method
Yan ZHOU ; Lijie DU ; Xuya ZHENG ; Feifei SUN ; Zhenling ZHANG
China Pharmacy 2020;31(17):2087-2093
OBJECTIVE:To compare the difference in the antioxid ant effect of fresh Rehmannia glutinosa ,dried R. glutinosa , R. glutinosa preparata during ancient characteristic processing and its polysaccharides before and after processing on aging model rats,and to provide reference for the processing of R. glutinosa . METHODS :The sample of R. glutinosa preparata was prepared according to ancient characteristic method. During the processing ,the fresh and dried R. glutinosa samples were retained. Then crude polysaccharide were extracted from fresh R. glutinosa and Rehmanniae radix preparata by water extraction and alcohol precipitation. Totally 96 rats were divided into blank group (water),model group (water),positive control group [vitamine C ,100 mg/(kg·d)],fresh R. glutinosa group [ 700 mg/(kg·d)],dried R. glutinosa group [ 135 mg/(kg·d)] ,Rehmanniae radix preparata group [ 135 mg/(kg·d)],fresh R. glutinosa polysaccharide group [ 1 400 mg/(kg·d),by the weight of fresh R. glutinosa ] and Rehmanniae radix preparata polysaccharide group mg/(kg·d),by the weight of Rehmanniae radix preparata] , with 12 rats in each group. Except for blank group ,other 制。E-mail:zhouyan1221@163.com groups were given D-galactose [ 125 mg/(kg·d)] on neck and back to induce sub-acute aging model. At the same time ,they were given relevant medicine in tragastrically,once a day ,for consecutive 56 days. After last admin istration,the liver ,brain,kidney,spleen,heart and thymus indexes were determined. The total antioxidant capacity (T-AOC),superoxide dismutase (SOD)activity,catalase(CAT)activity and MDA content in serum , liver,brain and kidney were determined. RESULTS :Compared with blank group ,organ indexes of rats in the model group were decreased significantly (P<0.05 or P<0.01);T-AOC,SOD activity and CAT activity in serum ,brain,liver and kidney tissue were decreased significantly (P<0.01),while MDA content increased significantly (P<0.01). Compared with model group ,the organ indexes of brain ,liver and kidney ,SOD activity in serum and kidney of fresh R. glutinosa group were not significantly increased (P>0.05);kidney index ,T-AOC in serum and brain ,SOD activity in serum ,liver and kidney tissue were not significantly increased in the dried R. glutinosa group(P>0.05);kidney index ,T-AOC in serum and cerebral tissue ,SOD activity in serum were not significantly increased in fresh R. glutinosa group(P>0.05);other organ indexes ,T-AOC,SOD activity and CAT activity in serum and tissues were increased significantly in other groups (P<0.05 or P<0.01),while MDA content in serum and tissues were decreased significantly in all administration groups (P<0.05 or P<0.01). Compared with fresh R. glutinosa group,T-AOC in serum was decreased significantly in dried R. glutinosa group(P<0.01),and there was no significant difference in other indexes (P>0.05);kidney and spleen indexes of rats in Rehmanniae radix preparata group were increased significantly (P<0.05),T-AOC in renal tissue ,SOD activity in serum ,cerebral tissue and renal tissue ,CAT activity in cerebral and liver tissue were increased significantly (P<0.05 or P<0.01),while MDA in cerebral and liver tissue were significantly decreased (P< 0.01). CAT in cerebral tissue and liver tissue of rats in Rehmanniae radix preparata group were significantly higher than those in positive control group (P<0.01). Compared with fresh R. glutinosa polysaccharide group ,spleen and renal indexes of rats in Rehmanniae radix preparata group were increased significantly (P<0.05 or P<0.01),T-AOC,SOD activity and CAT activity in serum and cerebral ,liver,renal tissues were increased significantly (P<0.05 or P<0.01). T-AOC and CAT activity of cerebral , liver and renal tissues in Rehmanniae radix preparata group were all significantly higher than those in positive control group (P< 0.05 or P<0.01). CONCLUSIONS :In the aspect of increasing organ index and improving the activity of antioxidant enzymes in serum,cerebral,liver and
2.Comparison on Changes of Volatile Components in Gecko Before and After Processing by HS-SPME-GC-MS
Xuya ZHENG ; Feifei SUN ; Lijie DU ; Yasen HUANG ; Zhenling ZHANG
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(15):145-152
ObjectiveOn the basis of sensory evaluation, the changes of volatile components in gecko before and after processing were compared, and the odor correction effect of different processing methods of gecko was discussed. MethodRaw products, fried yellow products, vinegar processed products, wine processed products, talcum powder scalding products and white wine sprayed products after scalding talcum powder of gecko were prepared, and 10 odor assessors were invited to evaluate the 6 samples in turn by sensory evaluation. Headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and relative odor activity value (ROAV) were used to analyze the key odor components, and multivariate statistical methods were used to analyze the difference of volatile components between raw and processed products of gecko. Taking water-soluble extract and protein contents as internal indicators, sensory evaluation score and content ranking of differential components as external indicators, and assigning a weight of 0.25 to them respectively, the comprehensive scores of raw products and processed products of gecko were calculated to evaluate the odor correction effect of each processing method. ResultThe average sensory evaluation scores of the raw products, fried yellow products, vinegar processed products, wine processed products, talcum powder scalding products and white wine sprayed products after scalding talcum powder of gecko were 1.6, 5.2, 6.2, 6.1, 7.2 and 8.0, respectively. ROAV results showed that key components affecting odor of gecko were 2-ethyl-3,5-dimethylpyrazine, isovaleraldehyde, trimethylamine, 1-octen-3-ol, n-octanal, nonanal, 2-methylnaphthalene, γ-octanolide, 2-heptanone and phenol. Principal component analysis (PCA) significantly distinguished raw products from processed products. Orthogonal partial least squares-discriminant analysis (OPLS-DA) results showed that there were 16, 13, 16, 16, 16 differential components between raw products, fried yellow products, vinegar processed products, wine processed products, talcum powder scalding products and white wine sprayed products after scalding talcum powder of gecko. Among these differential components, there were 4 common components, namely, the contents of different odor components (2-methylnaphthalene and 2-ethyl-p-xylene) decreased, while the contents of different flavor components (2-decanone and 2,3,5-trimethylpyrazine) increased. The comprehensive scoring results showed that the odor correction effect of each processed products was in the order of talcum powder scalding products>wine processed products>vinegar processed products>fried yellow products>white wine sprayed products after scalding talcum powder. ConclusionTalcum powder scalding is a better method to improve the odor of gecko, and it can provide an experimental basis for the processing of gecko to correct the odor.
3.Application of next generation sequencing technology in Mendelian movement disorders.
Yumin WANG ; Xuya PAN ; Dan XUE ; Yuwei LI ; Xueying ZHANG ; Biao KUANG ; Jiabo ZHENG ; Hao DENG ; Xiaoling LI ; Wei XIONG ; Zhaoyang ZENG ; Guiyuan LI
Journal of Central South University(Medical Sciences) 2016;41(2):197-205
Next generation sequencing (NGS) has developed very rapidly in the last decade. Compared with Sanger sequencing, NGS has the advantages of high sensitivity and high throughput. Movement disorders are a common type of neurological disease. Although traditional linkage analysis has become a standard method to identify the pathogenic genes in diseases, it is getting difficult to find new pathogenic genes in rare Mendelian disorders, such as movement disorders, due to a lack of appropriate families with high penetrance or enough affected individuals. Thus, NGS is an ideal approach to identify the causal alleles for inherited disorders. NGS is used to identify genes in several diseases and new mutant sites in Mendelian movement disorders. This article reviewed the recent progress in NGS and the use of NGS in Mendelian movement disorders from genome sequencing and transcriptome sequencing. A perspective on how NGS could be employed in rare Mendelian disorders is also provided.
Alleles
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Genetic Linkage
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High-Throughput Nucleotide Sequencing
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methods
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Humans
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Movement Disorders
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diagnosis
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genetics
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Sequence Analysis, DNA
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Transcriptome