Objective To evaluate the ability of two sets parameters of the volome rendering technich(VRT)with multislice spiral CT(MSCT)to display the normal inner ear.Methods Fifty-eight inner ears of twenty-nine healthy volunteers were scanned and reconstructed with VRT.The images of VRT were recombinated with two sets(A,B)of parameters.Results The semicirculares、cochlea,vestibule,fenestra cochleae and vestibuli of inner ear could be visualized clearly by VRT images with"A"group's parameters.The vestibuli was displayed unsharply by VRT images with"B" group's parameters.Conclusions The double-threshold segmentation matching with manuleditor of VRT was successfully used to separate the inner ear from the temporal bone.And the semicirculares,cochiea,vestibule,fenestra cochleae and vestibuli of inner ear could be displayed clearly by VRT images of"A"group's parameters(threshold:256～765,935～1019,brightness:42,opacity:70).

Objective:To establish the ultra high performance liquid chromatography (UPLC) fingerprints of Mume flos at different flowering stages; To provide reference for the quality research of Mume flos.Methods:The fingerprints of Mume flos were established by UPLC method, and the common peaks were identified by high performance liquid chromatography high resolution mass spectrometry (LC-MS). Chemometrics analysis was carried out with the fingerprints' common peak area of plum blossom at different flowering stages as a variable. Semiquantitative analysis of changes in flavonoids and phenolic acids in Mume flos at different flowering stages was conduct using peak area calculation method.Results:Totally 31 common peaks were identified in the fingerprints of plum blossom medicinal materials at different flowering stages and 9 components were identified. Clustering analysis (HCA) and principal component analysis (PCA) both classified plum blossom medicinal herbs at different flowering stages into three categories. Among them, there were significant differences between the groups at the bud stage, blooming period, and final flowering period, while the differences between the groups at blooming period and final flowering period were relatively small. The orthogonal partial least squares discriminant analysis (OPLS-DA) screened 16 different components with VIP>1.0. The contents of phenolic acids in different flowering stages were as follows: bud stage>blooming period>final flowering period, while the contents of flavonoids were as follows: blooming period>final flowering period>bud stage.Conclusions:This method is simple and reliable, and can provide reference for the quality evaluation of plum blossom medicinal materials at different flowering stages.