1.Discovery and etiological diagnosis of the first imported Chikungunya fever epidemic in Hunan province
Fangling HE ; Hengjiao ZHANG ; Juan WANG ; Jian HE ; Xuxiang LONG ; Bei LIU ; Rui YANG ; Shixiong HU ; Zhifei ZHAN ; Liang CAI ; Lidong GAO
Chinese Journal of Experimental and Clinical Virology 2020;34(2):175-179
Objective:To confirm the first imported Chikungunya fever (CHIK) epidemic in Hunan province.Methods:Serum samples of patients and colleagues were collected. The nucleic acids of Dengue virus (DENV), Yellow fever virus (YFV), Chikungunya virus (CHIKV) were detected by real- time fluorescent quantitative PCR. The positive PCR products were sequenced. Phylogenetic tree was constructed.Results:The serum samples of the patient and one of the five colleagues were positive for CHIKV. The Blast comparison of gene sequence showed 99% homology with CHIKV sequences. The infected CHIKV belonged to ECSA genotype in the phylogenetic tree.Conclusions:The first imported CHIK epidemic in Hunan province was confirmed through the epidemiological survey and etiologic detection.
Result Analysis
Print
Save
E-mail