1.Design and optimization of wireless power and data transmission for visual prosthesis.
Xuping LEI ; Kaijie WU ; Lei ZHAO ; Xinyu CHAI
Chinese Journal of Medical Instrumentation 2013;37(6):427-431
Boosting spatial resolution of visual prostheses is an effective method to improve implant subjects' visual perception. However, power consumption of visual implants greatly rises with the increasing number of implanted electrodes. In respond to this trend, visual prostheses need to develop high-efficiency wireless power transmission and high-speed data transmission. This paper presents a review of current research progress on wireless power and data transmission for visual prostheses, analyzes relative principles and requirement, and introduces design methods of power and data transmission.
Electric Power Supplies
;
Prosthesis Design
;
Visual Prosthesis
;
Wireless Technology
2.Optimum design and performance evaluation of down-sampling algorithm for visual prosthesis imaging processing.
Yun GU ; Kaijie WU ; Xuping LEI ; Hao WU ; Xinyu CHAI
Chinese Journal of Medical Instrumentation 2013;37(1):33-36
According to analyses of the real-time performance of the visual prosthesis image processing system, down-sampling is a key factor which influences the processing speed. Based on these analyses, the algorithm of grid sampled averaging was proposed for down-sampling. Then the effect of grid parameters on the result and algorithm complexity was evaluated by using the regional-averaging algorithm as a reference. Our research shows that the grid-averaging algorithm could reduce the computation burden by 90% or more, with no significant difference from the regional-averaging algorithm.
Algorithms
;
Image Processing, Computer-Assisted
;
methods
;
Visual Prosthesis
3.Construction of a temperature-sensitive and replication-defective T-vector and its application for gene knockout in Salmonella pullorum.
Chun GUO ; Lei LIN ; Nini REN ; Keran JIANG ; Haixia YUAN ; Xuping YU
Chinese Journal of Biotechnology 2012;28(9):1118-1129
Knockout is an important method for gene function study, while vector is the core of gene knockout. In order to obtain an effective vector for rapid construction of mutant and essentiality identification of the corresponding gene, we constructed a recombinant plasmid named plDM-T based on the temperature-sensitive and replication- defective plasmid plDM1 by inserting an Xcm I adapter into the EcoR I and Pst I sites of pIDM1. Digesting with Xcm I, pIDM-T can be prepared as a linear T-vector for PCR products cloning and be used to knockout the corresponding gene in the genome with insertion duplication mutagenesis. After the verification of temperature sensitivity of the replication of the plasmid, we cloned two Salmonella pullorum genes eno and ybdr into the constructed pIDM-T, and two recombinant plasmids pIDM-T_eno and pIDM-T_ybdr were identified. The recombinant plasmids were then transformed into S. pullorum strain CVCC527 and the IPC (Integration rate per cell) values were calculated. As a result, we identified the eno gene as an essential gene and the ybdr as a non-essential gene in CVCC527. We verified the correctness of recombination site in ybdr recombinant 527 clones (Sal delta ybdr) by PCR and sequencing. The pIDM-T vector can be used for gene knockout in S. pullorum, as well as the identification of essentiality of the corresponding genes, which offers an effective and rapid tool for gene function study in Salmonella.
Cloning, Molecular
;
Gene Knockout Techniques
;
Genetic Vectors
;
genetics
;
metabolism
;
Plasmids
;
genetics
;
Recombinant Proteins
;
genetics
;
metabolism
;
Recombination, Genetic
;
Salmonella
;
classification
;
genetics