OBJECTIVE:To establish the quality standard of Jinwu jiangu capsule. METHODS:Microscopy was used to identi-fy Panax notoginseng;TLC was used to identify Paeonia lactiflora and Sinomenii Caulis;HPLC was used to determine the content of paeoniflorin. The column was ZORBAX SB-C18 with mobile phase of acetonitrile-0.1% phosphoric acid(13∶87,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 30 ℃ and injection volume was 10 μl. RESULTS:Microscopy showed P. notoginseng had obvious features;P. lactiflora and Sinomenii Caulis in preparation were well-separated with clear spots. The linear range of paeoniflorin was 0.014-1.412 μg/μl(r=0.999 2);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 95.73%-99.48%(RSD=1.54%,n=6). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the quality control of Jinwu jiangu capsule.

OBJECTIVE:To explore the protective effects of Tongmaitang yanming capsules on retinal damage of aged rats. METHODS:Aged rats(aged 24 months)were randomly divided into Tongmaitang yanming capsule high-dose,medium-dose and low-dose groups (1.750,0.875,0.378 g/kg),positive control drug group (Calcium dobesilate capsules,0.175 g/kg) and model group,with 10 rats in each group. They were given relevant medicine intragastrically once a day for consecutive 90 days. At the end of the 90th day,another 10 rats aged 1.5 months were included into blank control group. After medication,60 rats were provid-ed with water but no food for 12 hours. Blood sample was collected from femoral,and the serum levels of GSH-Px,GSH,SOD and MDA was determined,respectively;right eyes were sampled,pathological change of retina was observed by HE staining and the number of retinal cell layer (RCL) neurons was counted. RESULTS:Compared with blank control group,serum levels of GSH-Px,GSH and SOD decreased in model group,while MDA level increased;the number of RCL neurons was (59 ± 4)% of blank control group (P<0.05 or P<0.01),and retina damage was found. Compared with model group,the serum levels of GSH-Px,GSH and SOD increased in positive control drug group and Tongmaitang yanming capsule groups,while MDA level de-creased;the number of RCL neurons in those groups were (98 ± 7)%,(69 ± 5)%,(78 ± 7)% and (95 ± 6)% of blank control group,respectively (P<0.05 or P<0.01);retina damage was relieved. CONCLUSIONS:Tongmaitang yanming capsules can in-hibit aging-induced lipid peroxidation of rat retina,prevent the progressive loss of retina neurons and protect aging-induced retina damage of rats.

Objective:To investigate the status of viral reservoirs in prostate tissue of patients with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS), and to investigate the effect of highly active anti-retroviral therapy (HAART) on HIV-1 DNA in prostate tissue of HIV/AIDS patients.Methods:Twelve patients with HIV infection and hyperplasia of prostate who required surgical treatment and admitted to Guangzhou Eighth People′s Hospital from July 2017 to October 2019 were included. Blood and prostate specimens of these patients were collected, and HIV-1 RNA in plasma, CD4 + T lymphocyte count in peripheral blood and HIV-1 DNA level in prostate tissue were tested respectively. The independent sample t test or Mann-Whitney U test was used for statistical analysis. Results:Among the 12 patients, the CD4 + T lymphocytes was (519.8±121.5)/μL and HIV-1 DNA in the prostate tissue was 2 602 (365, 10 700) copies/10 6cells in six patients who had not started HAART. The CD4 + T lymphocytes was (182.8±69.7)/μL and the HIV-1 DNA in the prostate tissue was 144 (36, 563) copies/10 6cells in the six patients who underwent HAART for over six months. There were statistically significant differences in CD4 + T lymphocytes and HIV-1 DNA in the prostate tissue between the two groups ( t=-5.889 and Z=-2.082, respectively, both P<0.05). Conclusion:Prostate tissue can be used as an HIV-1 virus repository with or without HAART, and the size of the prostate tissue virus repository can be reduced by HAART after immune reconstitution.

OBJECTIVE： To study the improvement effect of Shenrong bunao capsule on learning and memory ability of Alzheimer’s disease （AD） model mice， and to investigate its mechanism. METHODS： Totally 72 mice were randomly divided into blank control group （normal saline）， model group （normal saline）， Piracetam tablets group （positive control，0.80 g/kg），Shenrong bunao capsule high-dose，middle-dose and low-dose groups （1.92， 0.96， 0.48 g/kg）， with 12 mice in each group. Except that blank control group was given constant volume of normal saline subcutaneously. Other groups were given D-galactose （150 mg/kg） subcutaneously and sodium nitrite （50 mg/kg） intraperitoneally every day to induce AD model. At the same time，they were given relevant medicine intragastrically，once a day， for consecutive 60 d. 1 h after last administration， Morris water maze test was used to measure escape latency and times of crossing the platform within 90 s. HE staining was used to observe pathological changes of cerebral cortex in mice. Immunohistochemistry was used to detect the expressions of TNF-α， NF-κB p65， PI3K and Akt in cerebral cortex of mice. RESULTS： Compared with blank control group， escape latency was prolonged significantly （P＜0.01）， and the times of crossing the platform within 90 s was decreased significantly in model group （P＜0.01）. The neurons in cerebral cortex was damaged obviously， and the number of intact neurons was decreased significantly （P＜0.01）. The protein expressions of TNF-α， NF-κB p65， PI3K and Akt in cerebral cortex were increased significantly （P＜0.01）. Compared with model group， except that there was no statistical significance in escape latency， protein expressions of TNF-α， NF-кB p65， PI3K and Akt in Shenrong bunao capsule low-dose group （P＞0.05）， above indexes of other administration groups were improved significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： Shenrong bunao capsule can improve the learning and memory ability of AD model mice， and its mechanism may be related to the inhibiting the protein expressions of TNF-α，NF-κB p65， PI3K and Akt in cerebral cortex region and relieving inflammation injury so as to protect cranial nerve.