1.Simultaneous determination of six flavonoids in Hyperici Japonici Herba by HPCE-DAD.
Ke HAN ; Xunhang LIU ; Qinan WU ; Junsong LI ; Baochang CAI ; Lijuan WANG ; Xingsheng FU ; Aiping LU
China Journal of Chinese Materia Medica 2011;36(16):2219-2225
OBJECTIVETo establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin, isoquercitrin, hyperoside, quercitrin, kaempferol and quercetin in Hyperici Japonici Herba.
METHODBased on the mode of capillary zone electrophoresis, 40 mmol x L(-1) borax was used as buffer solution (pH 8.62), uncoated fused silica capillary (56 cm x 64.5 cm x 75 microm) was used, separation voltage was 25 kV, detection wavelength was at 206 nm, column temperature was maintained at 25 degrees C, and sample was injected at 50 mbar, 8 s.
RESULTSix flavonoids showed good linearity (r > 0.9953) in the range of the tested concentration, the average recoveries of the method were between 98.8%-102.9%.
CONCLUSIONThe method is simple, accurate and reproducible, and can be used for quality control of Hyperici Japonici Herba.
Electrophoresis, Capillary ; methods ; Flavonoids ; analysis ; Hydrogen-Ion Concentration ; Hypericum ; chemistry ; Temperature
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