Objective To study the changes and clinical significance of glutathione redox-state in patients with coronary heart disease (CHD). Methods Based on both with or without atherogenic risk factors and the result of coronary arteriography, 118 patients were calssified into three groups: control group( n =30) ,risk group(n =28) and CHD group (n =60). Venous blood sample was obtained, then the plasma reduced glutathione ( GSH) and oxidized glutathione (GSSC) were measured by using glutathione reductase recycling method. Redox potential(EhCSH/GSSG) was calculated using Nernst equation. According to clinical situation,CHD group was divided into two subgroups:un-stable angina pectoris( UAP) group(n = 32) and acute myocardial infarction (AMI) group (n =28). Results From control group to risk group,then to CHD group,GSH gradually decreased,and there was significant difference between CHD group and control group (P < 0.05 ) , but GSSG and EhGSH/GSSG gradually increased, there was significant difference between CHD group and risk group and control group (P < 0. 05). There was no significant difference be-tween the UAP subgroup and AMI subgroup in GSH,GSSG and EhGSH/GSSG(P >0.05). Conclusions Body's an-tioxidant capacity decreases and the oxidative stress exists in artherosclerosis, and furthermore there is a certain degree of relevance between oxidative stress and hyperlipoidemia,all these show that improving this situation may decelerate or prevent the formation and progress of artherosclerosis.

Objective To study the relationship between the level of uric acid and oxidative stress in patients with chronic systolic heart failure (CSHF). Methods One hundred and nine hospitalized patients from June 2006 to March 2007 were selected. Based on organic heart disease and heart function, they were classified into two groups: CSHF group (81 cases) and control group (28 eases). According to NYHA heart function grade (Ⅱ, Ⅲ, Ⅳ), CSHF group was divided into three subgroups. The plasma concentration of GSH and GSSG was determined by using glutathione reduetase recycling method (GR-DTNB). Redox potential (Eh) was calculated using Nemst equation according to the concentration of reduced and oxidized glutathione. Results There was significant difference between control group and CSHF group in the level of uric acid [(310.54±99.92) μ mol/L vs (499.09±168.04) μ mol/L], P < 0.01. Uric acid had a negative correlation with LVEF (r=-0.247, P=0.026), and a positive correlation with LVED D (r=0.266, P=0.016). The concentration of uric acid had a negative correlation with GSH (r=-0.328,P=0.003), and a positive correlation with GSSG (r=0.244, P=0.028) and Eh (r=0.309, P=0.005). Conclusions The concentration of uric acid increases in CSHF patients and has a correlation with LVEF and LVEDD. It may be a supplementary marker to reflect heart function and the serious degree of CSHF. The concentration of uric acid has a negative correlation with GSH, and a positive correlation with GSSG and the value of Eh. It may be used as an indicator of oxidative stress concerning its metabolic pathway.

Objective To investigate the changes and correlation between platelet activation and inflammatory response in patients with essential hypertension(EH). Methods Sixty-one patients with essential hypertension and sixty-four patients with paroxysmal supraventricular tachycardia as control group were enrolled in the study. The levels of high sensitivity C reactive protein ( hs-CRP ) and mean platelet volume ( MPV ) were measured and compared between the control group and the essential hypertension group. Results Compared with the control group,the levels of hs-CRP and MPV in the EH group were significantly higher( [3.51 ± 1.95]mg/L vs [ 1.15 ± 0. 77 ] mg/L, P ＜ 0. 05 ). The MPV in the EH group was significantly higher than that in the control group ( [ 11.29 ± 2. 18 ] flvs [ 9. 20 ± 2. 31 ] fl, P ＜ 0. 05 ). The level of hs-CRP positively correlated to the level of M PV (r = 0. 452, P = 0. 003 ). Conlusions There were inflammatory response and platelet activation in patients with EH and the serum levels of hs-CRP and MPV is correlated with the development of essential hypertension.

Although lymph node metastasis (LNM) is one of the most important contributory factors to the overall survival of ICC patients,the role of lymph node dissection (LND)is still under research.Some researchers thought hepatectomy combined with extended lymphadenectomy is the standard surgical treatment for ICC.However,not all the clinical centers approved routine LND.Some centers have reported the use of selective LND and limited routine LND.This review will mainly concern lymph node metastasis and lymph node dissection.

Combining abdominal regional anatomy with clinical knowledge, particularly with the acute abdomen and the abdominal surgery can raise the students’ interest and enthusiasm in the study, and raise the quality of teaching. It also can lay a solid foundation and construct a convenient bridge for the students' studying clinical subjects and working at the clinical work in future.

Abstract Objective: To construct and express anti-IL-2R? genetically engineering antibody Fab and identify its biological activity.Methods: PCR were used to construct cloning vector 5G1-pA22 of anti-IL-2R? antibody Fab,by digestion of vector 5G1-pA22, anti-IL-2R? an-tibody Fab gene fragment was obtained and then this fragment was coupled with digesting product of pET28b plasmid by EcoRI and HindIII toget a recombinant expression vector 5G1-pET28b, it was highly expressed in the E. coli BL21(DE3) in the form of inclusion bodies, compoing20% of the total protein. After the renaturation of antibody Fab, its binding activity was identified by ELISA and antibody competitive inhibitionassay in vitro. Results: Anti-IL-2R? antibody Fab was successfully constructed and highly expressed in the form of inclusion bodies, its yield wasup to 1 mg/ml.When applied to ELISA, antibody Fab exhibited good spectfic reactivity with rIL-2R, antibody competitive inhibition assay invitro indicated that antibody Fab can compete with IL-2 for binding IL-2R in activated T cell surface.As a result,the activation and proliferationof T cells were inhibited and inhibition percentage exceeded 90%. Conclusion: Had successfully constructed and expressed anti-IL-2R? geneti-cally engineering antibody. Fab,it had good biological ativity.

Objective:To observe the antibacterial activity of different preparations of 10 kinds of Chinese traditional medicine to Taphylococcus aureus,Staphylococcus epidermidis,Bacillus coli,Bacillus typhosus and Bacillus aeruginosus in vitro-experiment. Methods:Liquid agar dilution method was used. Results:The antibacterial activity of coptis chinensis is the best,scutellaria is the second. The antibacterial activity of tincture is better than that of decoction. Conclusions:The antibacterial activity of coptis chinensis is best during the 10 kinds of Chinese traditional medicine,and that of tincture is the best during different preparations.

Female ; Humans ; Hydrogen ; Hypoxia-Ischemia, Brain ; diagnosis ; Infant, Newborn ; Magnetic Resonance Spectroscopy ; methods ; Male

Objective To evaluate endos copy combined with peritoneal lavage for the treatment of severe acute biliogen ic pancreatitis(SAP).Methods 62 cas es of SAP were randomized into endoscopic intervention group (32 cases) and con trol group (30 cases) treated conservatively. The endoscopic group were treated with ERCP and EST,and BUS guided abdominal paracentesis drainage and antibiotic lavage. Abdomi nal pain, amylase level in blood and urine, WBC counts, average hospital stay, t he operation rate within one month, complication, mortality and curative rate in patients of the two groups were recorded and compared.Results Results in endoscopic group were superior to contro l group (all P

ObjectiveTo investigate the impact of blood glucose,blood pressure and blood uric acid level on hematuria in patients with acute coronary syndrome (ACS).MethodsOne hundred and sixty-two ACS patients were selected and received standardized treatment after admission to hospital.Urine test was taken and patients were divided into no hematuria group(37 cases),microscopic hematuria group (56 cases) and gross hematuria group(69 cases) according to the results.Blood pressure,fasting and postprandial 2 hours blood glucose,glycated hemoglobin and blood uric acid level were measured and compared among three groups.ResultsMicroscopic hematuria group compared with no hematuria group,fasting and postprandial 2 hours blood glucose,glycated hemoglobin,systolic blood pressure and blood uric acid level raised 58.0％[(7.9 ±0.7) mmol/L vs. (5.0 ± 1.1) mmol/L],33.3％[(12.4 ±0.8) mmol/L vs.(9.3 ± 0.6 ) mmol/L ],48.2％ [ ( 8.3 ± 0.8 )％ vs.( 5.6 ± 0.5 )％ ],23.8％ [ ( 151.6 ± 7.0) mm Hg ( 1 mm Hg =0.133 kPa) vs.(122.5 ±9.9) mm Hg],29.2％ [(635.4 ±47.4) μmol/L vs.(491.8 ±83.4)μmol/L]respectively,there were significant differences (P ＜ 0.05 or ＜ 0.01 ).Gross hematuria group compared with microscopic hematuria group,the above mentioned indexes raised 16.5％[ (9.2 ± 1.1 ) mmol/L vs.(7.9 ± 0.7)mmol/L],30.6％[ ( 16.2 ± 1.8) mmol/L vs.( 12.4 ± 0.8) mmol/L],14.5％[ (9.5 ± 0.8)％ vs.(8.3 ± 0.8)％ ],18.8％[(180.1 ± 12.3) mm Hg vs.(151.6 ±7.0) mm Hg],34.6％[(855.5 ±74.5) μ mol/L vs.(635.4 ±47.4 ) μ mol/L ] respectively,there were significant differences ( P ＜ 0.05 or ＜ 0.01 ).Gross hematuria group compared with no hematuria group,the above mentioned indexes increased significantly(P＜ 0.01 ).The level of diastolic blood pressureamong three groups had no significant difference(P ＞ 0.05).The Logistic regression analysis showed that fasting and postprandial 2 hours blood glucose (r =3.175,P =0.001 ;r =0.906,P =0.001 ),glycated hemoglobin ( r =16.109,P =0.001 ),systolic blood pressure (r =0.429,P =0.003 ),blood uric acid level(r =1.317,P =0.004) were risk factors on hematuria after antiplatelet and anticoagulant therapy in ACS patients,the impact of glycated hemoglobin and fasting blood glucose on hematuria was stronger than that of blood uric acid level and systolic blood pressure.ConclusionWith the increase of blood glucose,systolic blood pressure,blood uric acid,the risk of hematuria increases in ACS patients.