To investigate into the effect of ultrasonic - spraying of Chinese Materia medica on respiratory failure, the remedy produced by our hospital was studies for the treatment of 27 cases of such disorder (type II). with another 30 cases treated by coramine as control. Results revealed, for the treatment group, total markedly effective rate was 70. 3%, SaO2 increased to 38. 4%. The rate of amelioration of coughing, dyspnea, sputum and average treating time for the treatment group were all significantly different from the control group (P

Objective To investigate the nursing for preventing deep venous thrombosis after mini-incision for total hip arthroplasty in old patients. Methods 53 old patients who accepted total mini-incision hip arthroplasty from July, 2005 to June, 2006 were analyzed retrospectively. Results Affected limb swelling were found in 8 patients 2 d to 2 weeks after operation. They all were cured after anticoagulation treatment, no pulmonary embolism occurred.Conclusion Nursing intervention can prevent deep venous thrombosis after mini-incision for total hip arthroplasty.

Effects of 7 fungus strains on tobacco quality by applying fungal leavens on upper leaves were studied. Results showed that BF03, BF06 and BF63 can remarkably change the chemical components of tobacco, such as soluble sugar, protein, nicotine etc., and make them more harmonious. And the smoking quality of the flue-cured tobacco leaves treated was much better than the control.

Objective To investigate the clinical outcomes of minimally invasive percutaneous nephrolithotomy(MPCNL)in the treatment of lower caliceal calculi.Methods We retrospectively re- viewed the clinical outcomes and complications of 33 patients who underwent MPCNL for lower caliceal cal- culi from March 2001 to April 2005.The average diameter of the calculi was 2.8 cm.Single tract nephrosto- my was performed in all 33 cases;among them renal access was obtained through a middle calyx in 10 cases and a lower calyx in 23.Nine cases had F14 renal access;and 24 cases,F16.Results Of 33 cases,28 (85%)achieved stone-free at 1 session.A second-look was needed in 3 cases due to intraoperative bleed- ing;ESWL,in 1 case with residual,calculi;no treatment,in 1 case with residual calculi＜4 ram.The mean operative time was 93 min;mean blood loss was 113 ml;mean hospital stay was 11 d.Blood transfusion was needed in 1 patient who suffered from hepatic cirrhosis preoperatively;another experienced severe bleeding 7 d after operation and was cured with hyperselective spongia gelatinosa embolization of the renal artery.Fol- low-up was available in 19 cases for 2-48 months,and no recurrence of renal calculi was noted.Conclu- sions Minimally invasive percutaneous nepbrolithotomy has advantages of safety,less invasion,and easy re- covery for the treatment of lower caliceal calculi.

OBJECTIVETo evaluate the initial clinical characteristics, the response to treatment, and the outcome in adult patients with Evans syndrome.

METHODSThe clinical data of 84 adult patients (20 males, 64 females) with Evans syndrome diagnosed at our center between 1984 and 2007 were retrospectively analyzed.

RESULTSThe patients were followed up for a median duration of 17.5 (0.03 - 140) months. All the patients initially received intravenous steroids with or without intravenous immunoglobulin (IVIG). Forty-seven patients were treated with corticosteroids alone initially. Complete remission (CR) and partial remission (PR) were achieved in 38 of the patients, but 92.1% of them relapsed during a median follow-up of 12 months. Twenty-eight patients who were resistant to corticosteroids therapy or with severe bleeding were subsequently administered immunosupressive agents. CR and PR were obtained in 89.3% of them. Within a median follow-up of 8 months, 84% of these patients relapsed.

CONCLUSIONSEvans syndrome is a chronic and easy to recurrent disease, which is often refractory to conventional therapy. Treatment with combination agents might be a useful therapeutic approach to the patients.

Adrenal Cortex Hormones ; Adult ; Anemia, Hemolytic, Autoimmune ; Follow-Up Studies ; Humans ; Remission Induction ; Retrospective Studies ; Treatment Outcome

Objective To investigate the prevention effect of intermittent pneumatic compression pump on DVT in patients undergoing knee joint replacement.Methods A total of 318 patients with knee joint replacement in our hospital were selected as study objects,and 108 cases with routine nursing were as control group,and 210 cases with intermittent pneumatic compression were as observation group.The changes of D-D,PLT and Hb,and DVT of two groups were compared.Results PLT and D-D after 6 d of the operation in the observation group was lower than that in the control group,the difference was statistically significant(P ＜0.05).Venous velocity of lower limb after 6 d of operation in the observation group was quicker than that in the control group,the detection rate of DVT was lower than that in the control group,DVT diameter was less than that in the control group,the differences were statistically significant (P ＜ 0.05).Conclusion Intermittent pneumatic compression pump can reduce PLT,D-D and the incidence of DVT after knee replacement.

Objective To investigate the prevention effect of intermittent pneumatic compression pump on DVT in patients undergoing knee joint replacement.Methods A total of 318 patients with knee joint replacement in our hospital were selected as study objects,and 108 cases with routine nursing were as control group,and 210 cases with intermittent pneumatic compression were as observation group.The changes of D-D,PLT and Hb,and DVT of two groups were compared.Results PLT and D-D after 6 d of the operation in the observation group was lower than that in the control group,the difference was statistically significant(P ＜0.05).Venous velocity of lower limb after 6 d of operation in the observation group was quicker than that in the control group,the detection rate of DVT was lower than that in the control group,DVT diameter was less than that in the control group,the differences were statistically significant (P ＜ 0.05).Conclusion Intermittent pneumatic compression pump can reduce PLT,D-D and the incidence of DVT after knee replacement.

AIMTo elucidate the molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA).

METHODSFlow cytometry was used to determine the cell cycle changes of HL-60 cells upon ATRA treatment. Gene expression profiles of HL-60 cells induced by 1 mumol.L-1 ATRA were obtained by using cDNA microarrays containing 9,984 genes and expressed sequence tags (ESTs).

RESULTSCell cycle analysis showed that 48%-73% of cells were arrested at G1/G0 phase upon ATRA treatment; cDNA microarray results demonstrated that the expression of genes encoding adhesion molecules, tissue remodeling proteins, transporters and ribosomal proteins were up-regulated in ATRA treated of HL-60 cells. Several genes involved in oxidase activation pathway were also differentially expressed.

CONCLUSIONATRA treatment induced growth arrest and differentiation in HL-60 cells, which is associated with regulation of the oxidase activation pathway and the expression of tissue remodeling proteins.

Antineoplastic Agents ; pharmacology ; Cell Cycle ; Cell Differentiation ; Gene Expression Profiling ; Granulocytes ; drug effects ; pathology ; HL-60 Cells ; Humans ; Oligonucleotide Array Sequence Analysis ; Tretinoin ; pharmacology

OBJECTIVETo study the emotional and depressive differences between severe acute respiratory syndrome (SARS) patients whose occupations were doctor/nurse and others.

METHODSDuring the three months when SARS was explosive in 2003, 524 questionnaires were collected from Xuanwu Hospital, You'an Hospital, Xiaotangshan Hospital, Renmin Hospital and Ditan Hospital in Beijing. There were 109 questionnaires finished by patients as doctors/nurses themselves. For a background matching, 109 questionnaires were also finished by the others.

RESULTSFrom 218 questionnaires, we found that the score on emotional condition (46.6204 +/- 8.4408 vs. 41.6789 +/- 8.612 95, P < 0.001) of SARS patients whose jobs were doctor/nurse was higher than the other groups on while the score of SARS patients whose jobs were doctor/nurse was lower than the other groups (37.7615 +/- 9.026 61 vs. 41.2844 +/- 9.655 25, P = 0.006) regarding depressive condition. The scores of emotional and depressive condition were all correlated with the factor as "I can not master my future".

CONCLUSIONDoctors/nurses having SARS had less emotional and depressive conditions than the others, which might due to the difference in medical knowledge, working condition and the route of infection, suggesting that psychological intervention in the post-SARS period called for attention.

Case-Control Studies ; China ; Cross Infection ; prevention & control ; psychology ; Depression ; psychology ; Female ; Health Personnel ; psychology ; Humans ; Logistic Models ; Male ; Patients ; psychology ; Severe Acute Respiratory Syndrome ; psychology ; Socioeconomic Factors ; Surveys and Questionnaires

OBJECTIVETo study the morphology and functional character of blood-spleen barrier (BSB) and establish the concept of BSB.

METHODSThirty healthy Wistar rats were studied. Ten rats were injected with 1.5 ml mixed fluid of India ink and physiological saline through the tail vein. Histological changes of the spleen in all animals were observed with light and electron microscopy, including HE, Foot, Masson staining and immunohistochemistry of CD68 and CD34.

RESULTSMost of the carbon particles were within the splenic sinuses in marginal zone but not in the white pulp after 6 h. There was a characteristic distribution of the macrophagocytes, vessel endothelial cell, reticular tissue and collagen fiber in the BSB.

CONCLUSIONSBSB, surrounding the white pulp, is composed of macrophagocytes, marginal-sinus-endothelial cells and their basement membrane, the reticular tissue (reticular cells and reticular fibers) and collagen fibers. The role of BSB is to keep the microenvironment of white pulp stable. It becomes mature while the formation of germinal center of the white pulp. The permeability of BSB changes during its development.

Animals ; Basement Membrane ; ultrastructure ; Endothelial Cells ; ultrastructure ; Female ; Macrophages ; ultrastructure ; Male ; Rats ; Rats, Wistar ; Reticulocytes ; ultrastructure ; Spleen ; blood supply ; ultrastructure