The therapeutic response of anti-vascular endothelial growth factor (VEGF) differs among individuals.According to the changes of central retinal thickness,intraretinal fluid,subretinal fluid,best corrected visual acuity and other morphological or functional manifestations after treatment,the performance of the treated eyes can be classified as optimal response,poor response and non-response.A variety of factors could account for poor or non-response to anti-VEGF,such as genomic polymorphism and specific genomic risk alleles,lesion characteristics,vitreous and macular structural abnormalities,resistance to anti-VEGF drug,and the role of pericytes and others.The common counter measures include increasing the dosage,shortening the injection interval and replacing with another alternative drug,inhibition of pericytes,relieving vitreomacular anatomical abnormalities.It is still worthy of further exploration that how to assess individual reasons for non-response,so that we can give proper treatment to reduce the excessive use of anti-VEGF drugs and improve the clinical management of ocular neovascularization diseases.

Objective To optimize the extraction process of Ardisia crenata based on the content of total saponins and to provide a basis for the Ardisia crenata study. Methods Based on the results of the single-factor tests and the Box-Behnken central composite experimental design principles, a response surface methodology which has three factors and three levels was designed to optimize the extraction process of Ardisia crenata based on the content of saponins. Results A maximal extraction yield of total saponins reached 2. 29% under the optimal conditions as follows:70% alcohol was used as extraction solvent with the material to liquid ratio of 116, the extraction time was 3 h at 70 ℃. Conclusion The optimized extraction process is accurate, reliable and practically valuable.

Age-related macular degeneration (AMD) is the leading cause of low vision and blindness in the elder people.Various animal models based on the technology of the genetics are established recently in researches on this disease to investigate its pathogenesis or mechanisms.This article reviews different experimental genetic murine models of AMD used in recent studies.

Objective To verify the flow field performance of the air dynamic wind tunnel. Methods The performance of the flow field was evaluated by testing the flow speed of sample-point on the sample-transect of the tunnel. Results Among the flow range of the tunnel, the relative deviations of the speeds of each sample-point on the sample-transect were less than 10%. When the flow rate was set, the relative deviations of the sample-transect average speed in different test period were less than 10%. The ratio of average wind velocity of upper reach to down reach of the tunnel was between 0.92 and 1.03. Conclusion The flow field of the air dynamic wind tunnel is even, stable and coincident.

OBJECTIVE To analyse the bacterial culture of aqueous vitreous as well as their sensitivity to antibiotics in intraocular foreign bodies(IOFB).METHODS Bacterial culture of aqueous vitreous as well as their sensitivity to antibiotics were taken at 32 cases(32 eyes) of IOFBs with in 24 hours.And the results were analyzed.RESULTS 21 cases with bacterial culture of aqueous vitreous were found to be positive with bacterial infection.Staphylococcus epidermidis was the most frequently bacteria and identified in 15 cases,which accounted for 71.4% of these 21 cases.All the identified bacteria were sensitive to Ofloxacin,Ciprofloxacin and Cefoperazone,and partly sensitive to Gentamycin and Tobramycin.All of them were resistant to Chlororamphenicol and Rifapine.The occurrence of positive bacterial identification of unmetal foreign bodies were significantly higher than that of metal foreign bodies(?2=4.631,P=0.029).There was no significomt difference between.The location of penetrating eye trauma or lens rupture in the bacterial positive rate identification(?2=2.398,P=0.122;?2=0.327,P=0.568).CONCLUSIONS In patients with IOFB,the major bacteria entering into the eye was Staphylococcus epidermidis.As for prophylactic antibiotics,Quinolones and Cefoperazone can be used as the first line antibiotics.

Nitric Oxide Synthase ; Ventricular Remodeling

Methamphetamine ( METH ) , a powerful stimulant of amphetamine type in the central nervous system ( CNS ) , is a new kind of synthetic drugs mainly in psychological dependence. Long-time abuse will lead to strong neurotoxicity and drug de-pendence. Recently it has become the abuse problem in public health. Autophagy is a process of degradation and utilization of the cellular components by the lysosome, which widely exists in eukaryotic cells. Many studies show that methamphetamine can iduce autophagy,but the underlying mechanism is not fully un-derstood yet . Therefore,the mechanisms and signaling pathways of autophagy by methamphetamine and the roles of autophagy in methamphetamine induce neurotoxicity are reviewed in this pa-per, so as to provide reference materials for further research of methamphetamine and autophagy.

OBJECTIVE:To improve the rational and scientific drug purchase quantity planned by hospital pharmacy. METH-ODS:The deficiency in present drug purchase module of hospital information system(HIS)was analyzed;the formula of planned purchase quantity was improved by incremental coefficient method,ABC analysis method and VEN analysis method. The high and low stored values and packaging volume were set to adjust planned drug purchase quantity. Based on the computer program,a one-key-getting procedure was quasi established,and five typical drugs were selected for simulation. RESULTS:Based on previous formula,the parameters representing change trend,incremental coefficient k1 and inventory coefficient k2 were added in new formu-la. The one-key-getting procedure could calculate the quantity of all drugs by simply entering the planning cycle N1 and the safe peri-od N2 of the some category drugs into the system. After simulated operation practice,compared with pre-improvement,the occupa-tion of funds was significantly decreased and the quantity of drug was rounded off according to package in post-improvement. CON-CLUSIONS:Through function improvement of drug purchase module in HIS and the quasi establishment of one-key-getting pur-chase mode,planned drug purchase quantity is more reasonable,scientific and accurate. It ensures the clinical drug supply in time without causing a backlog,and effectively reduces the cost of manpower,material and capital.

Systematic pharmacological evaluation of material basis for the efficacy of Chinese herbal formula is one of the key issues in Chinese materia medica(CMM),which is in urgent need of solution.However,its scientific connotation is still controversial in academic circles.The material basis for the efficacy of Chinese herbal formula is the total non-endogenous bioactive compound(derived either directly or indirectly from this formula) exerting the therapeutic effects,which is generated by the interaction of the formula with human body and reaches certain concentration in the target organs or tissues.Systematic evaluation of material basis for the efficacy of Chinese herbal formula is the cornerstone of modernization and internationalization of CMM,while the quality control of CMM is its prerequisite.

Objective To investigate the expression level of serum Golgi protein-73 (GP73) in patients with primary hepatocellular carcinoma (HCC), or accompanied with Budd-Chiari syndrome (BCS), and to discuss its clinical value in making early diagnosis. Methods The peripheral serum samples were collected in 490 individuals, including patients with HCC (n = 65), cholangiocarcinoma (CC, n = 35), Budd-Chiari syndrome accompanied with HCC (n = 40), type B virus hepatitis (n = 60), cirrhosis after B hepatitis (n = 60), Budd-Chiari syndrome complicated by cirrhosis (n = 60), other malignant lesions (n =50), benign hepatic lesions (n = 60) and healthy volunteers (n = 60). The expression levels of serum GP73 and AFP were determined by using Elisa and electrochemiluminescence methods separately. Results The expression level of serum GP73 in the patients with HCC , CC or BCS plus HCC were significantly higher than that in the other individuals (P < 0.05). The area under the receiver operating characteristic curve (ROC), the sensitivity, the specificity and the diagnostic accuracy of GP73 and AFP for HCC were 0.868, 90.8%, 75.5%, 77.6% and 0.739, 60%, 83.2%, 80.2% respectively. The cut-off values of GP73 and AFP were 43.40 ng/ml and 20ng/ml respectively. The area under ROC and the sensitivity of GP73 for the diagnosis of HCC were strikingly higher than those of AFP (P < 0.05), while no significant differences in the specificity and diagnostic accuracy existed between GP73 and AFP (P > 0.05). The area under ROC, sensitivity, specificity and the accuracy of GP73 for the diagnosis of CC were 0.774 , 88.6%, 72.7% and 73.9%respectively, and the optimal cut-off value was 45.40 ng/ml. Conclusion In diagnosing HCC, the expression level of serum GP73 is superior to AFP. It is hopeful that serum GP73 may be proved to be an early serum tumor marker for HCC.