Objective To explore the characterization of antibiotic resistance in hospitalized elderly patients with lower respiratory tract infection caused by Pseudomonas Aeruginosa ,and provide scientific basis for clinical ra-tional use of antimicrobial drugs and prevent the emergence of pan-drug resistant bacteria .Methods The drug sensi-tive tests of 347 hospitalized elderly patients with lower respiratory tract infection caused by Pseudomonas Aeruginosa in Shandong provincial hospital affinited to Shandong university from May 2010 to June 2012 were analyzed retrospec-tively.Results The antimicrobial resistance of the 347 cases was rather serious .The resistant rates of ampicillin and cefazolin were both 100.0%.Followed by ampicillin/sulbactam,tobramycin,sulfamethoxazole/trimethoprim,the re-sistant rates were 95.1%,86.8%and 83.6%respectively.The resistant rate of imipenem has reached 19.6%,and the detection rate of pan-drug bacteria was 4.9%.Conclusion The antibiotic resistance in hospitalized elderly pa-tients with lower respiratory tract infection caused by Pseudomonas Aeruginosa has become more and more serious .In order to prevent the emergence and prevalence of pan-drug resistant bacteria , antibacterial agents should be chosen reasonably according to the results of drug sensitive tests .

Acinetobacter baumannii.Sixty(20.6%) strains of Gram-positive bacteria were isolated.Staphylococcus aureus was the most predominat pathogen(13.0%),meticillin-resistant S.aureus(MRSA)(72.8%).Twenty-nine(10.0%) strains of fungi were isolated,Candida albicans was the most predominant(7.56%).The result of drug sensitive test showed that there were high rates of multiple drug resistance in most bacteria strains,but there were high different drug resistance rates to different antibiotics.Ampicillin had the highest resistance rate nearly 100%.In contrast,the imipenem resistance had the lower resistance rate from 0-14.3%.Lactamases(ESBLs) in K.pneumoniae and E.coli were 56.5% and 41.2%. CONCLUSIONS Gram-negitive bacteria are major pathogens in pneumonia of aged.There are high rates of multiple drug resistance in bacteria strains.The clinic should reasonably choose the antibiotics according to the test results of the pathogens sensitivity to drugs.

Objective To analyze distribution and drug resistance of MRSA separated,to probe into a treat method for MRSA infection and offer scientific gist for reasonable use of clinical antibiotic. Methods 568 MRSA separated from clinic were measured and a drug sensitivity test was performed by means of Kirby-Bautr agar diffusion method. Results 370 individual plants were filtered, the rate of inspection was 65.1%. MRSA was 36.2 % among them,MRSCON was 77.5% ,the drug resistance of MRSA to quinolones(21% ～48% ) was below MRSCON(84 %～89 % ). The drug resistance of MRSA and MRSCON obviously excelled MSSA and MSSCON ( P＜0.05 ). VRS was undiscovered. Conclusion Severe infection caused by MRS should choose vancomycin resistant enterococci to cure at fist. Staphylococcus was one of primary pathogenic bacterium caused infection in hospital, MRS had multiple drug resistance and measure range about drug resistance should be extended, which would advance the cognition about the drug resistance of antibiotic and cut off the transmitted path, reduce the drug resistance of bacteria and the infection rate in hospital.

Objective To investigate the distribution and drug resistance of pathogenic bacteria in hospital ICU patients with lower respiratory tract infection , and provide scientific reference for clinical rational drug use . Methods The strains were identified by VITEK -32 automatic bacterial identification instrument , the bacterial sensitivity was determined by K -B disk diffusion method , and the statistical analysis was performed by WHONET 5.4 software.Results The total isolated pathogenic bacteria of lower respiratory tract infection in ICU patients was 453 strains.332 strains of gram negative bacteria accounted for 73.3%,and the former three ones were Pseudomonas aeruginosa (115 strains,accounted for 25.4%),Klebsiella pneumonia (90 strains,accounted for 19.8%),Acineto-bacter baumannii (38 strains,accounted for 8.4%).102 strains of gram positive bacteria accounted for 22.5%,and the top three were Staphylococcus aureus (31 strains,accounted for 6.8%),coagulase negative Staphylococcus (22 strains,accounted for 4.9%),Enterococcus (18 strains,accounted for 4%).Meropenem,imipenem (Stenotroph-omonas maltophilia was excepted ) ,Cefoperazone /sulbactam and Amikacin were most sensitive against gram negative bacteria;Teicoplanin and vancomycin were highly sensitive against gram positive bacteria .Conclusion The main pathogenic bacteria of respiratory tract infection in ICU patients was gram negative bacilli ,which were seriously resist-ant to commonly used antimicrobial drugs .So strengthening the infection management of ICU and the control for risk factors,and rationally using of antimicrobial drugs has great significance in reducing the drug resistance of pathogenic bacteria rate .