Pre-hospital care is an important part of the health care system. Nowadays , there are many defects in the pre-hospital emergency charging method in China. It analyzes the effectiveness and impact of adjustment the charging method. There will be a preliminary study of hospital first aid fees adjustment of changing the current method from front-line staff receiving cash to entrusting the local hospital, charge with real-time settlement of health insurance for the convenience of the patient and reduce the workload of health insurance agencies, make financial emergency center management more standardized and efficient.

Objective To observe the effects of modified Jianpi Yishen Decoction on urinary osteopontin (OPN) in calcium oxalate nephrolithiasis patients after percutaneous nephrolithotomy (PCNL) or ureteroscope lithotomy (URL);To clarify the mechanism of modified Jianpi Yishen Decoction on the prevention of calcium oxalate kidney stones. Methods Totally 116 calcium oxalate nephrolithiasis patients were randomly divided into trial group (62 cases) and control group (54 cases). The trial group took modified Jianpi Yishen Decoction every other day, while the control group took potassium sodium hydrogen citrate granules three times a day. The concentrations of OPN, urinary calcium and urinary oxalic acid of the patients in the two groups were observed before treatment and 2 weeks and 4 weeks of treatment. Results The concentration of urinary OPN of 2 weeks and 4 weeks of the treatment in the trial group was significantly increased compared with before treatment (P<0.05), and the difference was statistically significant compared with the control group (P<0.05). The concentration of urinary OPN in the control group had no significant change after treatment (P>0.05). The differences in the concentrations of urinary calcium and urinary oxalic acid of the two groups between before and after treatment were not significant (P>0.05). Conclusion Modified Jianpi Yishen Decoction can effectively restrain the formation of the calcium oxalate stones by increasing the level of urinary OPN, which demonstrates effective prevention in the calcium oxalate nephrolithiasis patients after PCNL or URL.

Objective To explore the application effect of comprehensive nursing in the treatment of elderly diabetes patients.Methods A total of 80 elderly patients with type 2 diabetic patients were randomly divided into the observation group and the control group,with 40 cases in each group,the control group was given the routine nursing,the observation group was given com-prehensive nursing,treatment compliance,blood sugar control,self-care ability and nursing satis-faction were compared.Results The treatment compliance of diet control,emotional manage-ment,medication compliance in the observation group was higher than that in the control group, there were statistically significant (P <0.05).3 months after the intervention,FPG,2h PBG in the observation group was lower than that in the control group,there were statistically significant (P <0.05).Self concept,self responsibility and self care skills in the observation group was higher than that in the control group,nursing satisfaction was higher than that in the control group,there were statistically significant difference(P <0.05).Conclusion Comprehensive nursing can meet the nursing demand for elderly diabetes patients.It can improve thetreatment compliance and nurs-ing satisfaction.

Objective To explore the application effect of comprehensive nursing in the treatment of elderly diabetes patients.Methods A total of 80 elderly patients with type 2 diabetic patients were randomly divided into the observation group and the control group,with 40 cases in each group,the control group was given the routine nursing,the observation group was given com-prehensive nursing,treatment compliance,blood sugar control,self-care ability and nursing satis-faction were compared.Results The treatment compliance of diet control,emotional manage-ment,medication compliance in the observation group was higher than that in the control group, there were statistically significant (P <0.05).3 months after the intervention,FPG,2h PBG in the observation group was lower than that in the control group,there were statistically significant (P <0.05).Self concept,self responsibility and self care skills in the observation group was higher than that in the control group,nursing satisfaction was higher than that in the control group,there were statistically significant difference(P <0.05).Conclusion Comprehensive nursing can meet the nursing demand for elderly diabetes patients.It can improve thetreatment compliance and nurs-ing satisfaction.

The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA), the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins, whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS.
Blotting, Western ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; chemistry ; immunology ; Humans ; Nucleocapsid Proteins ; chemistry ; immunology ; Peptide Fragments ; chemical synthesis ; Plasmids ; Recombinant Proteins ; immunology ; isolation & purification ; metabolism ; SARS Virus ; genetics ; immunology ; metabolism