Transcranial doppler (TCD) is an important ultrasound technology for non-invasive cerebrovascular dynamic monitoring. It has been widely used in the evaluation of acupuncture efficacy and the mechanism studies of acupuncture effect. Based on the TCD measuring participants in the physiological or pathological conditions, acupuncture at different single points or groups of points, or different acupuncture methods, can cause overall benign regulatory effectoncerebral hemodynamics. The TCD can provide visual basis for clinical acupuncture scheme optimization. However, there are still some deficiencies in the setting of cerebral blood flow parameters, clinical design, and technical operation. We should start with the intereted diseases, conduct large sample RCT, pay attention to cross contrast with other cerebral blood flow detection technologies, and carry out standardized research on the influencing factors of Acupuncture manipulation, acupuncture Deqi, and stimulation parameters.

Objective To explore clinical effect of ureteroscope atmospheric pressure trajectory lithotriptor in treatment of the ureterolithiasis.Methods Application of atmospheric pressure trajectory lithotriptor to treat the 108 cases of ureterolithiasis through ureteroscope peeper.Results 103 cases(95.3%) got success in orthotopic lithotriptics,success rate of upper segment calculi in ureter was 83.3%,middle segment was 96.3%,lower segment was 98.4%,mean lithotriptic time was (45?2.5)min,100% stone was excreted 1～3 months after operation.Conclusion Ureteroscope at mospheric pressure trajectory lithotriptor in treatment of ureterolithiasis has merits of safe,effective,manipulation easiness,so it is perfect method for therapy of ureterolithiasis.

Objective:To investigate the mechanisms of decoy oligodeoxynucleotides(decoy-ODN) blockading Stat3 that inhibit breast cancer cells MDA-MB-231 proliferation.Methods:Stat3 decoy-ODN and scramble control were transfected into breast cell line MDA-MB-231, respectively. The cell proliferation capability was detected by cell counting; flow cytometry was applied to detect MDA-MB-231 cell cycle; FITC labeled decoy was observed by Reflected Light Fluorescence Microscope; the expression of the gene controlled by Stat3 was examined by means of RT-PCR and Western blot assay.Results:Stat3 decoy-ODN could be internalized into MDA-MB-231 cells and inhibit the growth of MDA-MB-231 cell via inducing its apoptosis. Stat3 decoy-ODN also could significantly reduce the expression of Stat3 controlling genes such as Bcl-xl,c-myc and CylinD1.Conclusion:Stat3 decoy-ODN can inhibit breast cell line MDA-MB-231 proliferation by blockading JAK/STAT pathway. This suggests that transcription factor decoy-ODN may serve as a novel therapeutic strategy for breast cancer.

Objective:To investigate the distribution of γδT17,Th17 and Tc17 cells in the lung of mice severely infected by influenza A(H1N1)pdm09 virus and the relationship between these cells with lung immunopathalogical injury.Methods:Intranasal infection was used to establish mouse model of severe H1N1 infection.Flow cytometry assay was used to detect the proportion and number of γδT17 cells,Th17 cells and Tc17 cells in the lung.The concentrations of interleukin-17A(IL-17A),interleukin-1β(IL-1β)and interleukin-23(IL-23) in the bronchoalveolar lavage fluid and serum were assayed by enzyme-linked immunosorbent assay and Lu-minex assay.Results:①The model of mice severely infected by influenza A(H1N1)pdm09 virus was established successfully.②The ratio of γδT cells,but not CD4+T and CD8+T cells in total lymphocytes of the lung of infected mice significantly increased compared with uninfected control mice at the third day post infection(DPI)(P<0.01).③The proportion and number of γδT17 cells,Th17 cells and Tc17 cells in total γδT cells,Th cells and Tc cells in the lung of infected mice were significant higher than that in uninfected control mice at the first DPI,respectively.However,the absolute number of γδT17 cells was far more than Th17 and Tc17 cells(P<0.05);④The concentration of IL-17A in BALF increased significantly after infection(P<0.05),and the concentration of IL-17A in serum increased significantly at the third DPI(P<0.05).The concentrations of both IL-1β and IL-23 in BALF probably participating in the activation of γδT17 cells increased significantly after infection compared with uninfected control mice.Conclusion:The γδT17 cells could be activated and secreted IL-17A via γδTCR non-depended pathway and involved in inflammatory pathological injury of lung at the early stage of severe H1N1 infection.

Objective To evaluate the pathological,histochemical and clinical features of primary clear cell carcinoma of the epididymis,and to investigate its diagnosis,treatment and prognosis.Methods The clinical data of one case of primary clear cell carcinoma of the epididymis were reported and the relevant literature was reviewed.Results The patient was a 43-year-old man presenting with a painless mass in the left epididymis.On physical examination,a 1.0 cm-sized smooth solid nodule on the head of left epididymis could be palpated without tenderness.Then the patient underwent left epididymectomy.On postoperative histopathological study,the tumor was characterized by diffuse sheets of clear cells and adenoid structures.Immunohistochemical assay showed AE(++),AE3(+/-),NSE(-) and interstitialoma(-) in the tumor tissues.Postoperatively,MRI showed no space-occupying lesion in both kidneys,and B-ultrasound indicated no abnormality of the bladder.Fifteen days after operation,left orchiectomy was performed on the patient.Pathology showed no clear cell carcinoma tissue of the testis.Follow-up was 15 months.The patient was reported to be alive and tumor-free.Conclusions The histogenesis of primary clear cell carcinoma of epididymis is unclear yet.The diagnosis depends on pathologic studies,and should be differentiated from nephrogenic adenoma and metastatic carcinoma.Radical surgery is the main treatment choice.

Objective To observe the relationship between CT signs, prognosis and the expression of CD44v3 and CD44v6 in peripheral non-small-cell lung cancer (NSCLC). Methods The expression of CD44v3 and CD44v6 was detected with immunohistochemistry (SP method) in 46 patients with peripheral NSCLC confirmed by surgery and pathology. The expression, CT signs and prognosis of NSCLC were analyzed respectively. Results The expression of CD44v3 and CD44v6 correlated with deep lobulation, spinous protuberant sign, short spiculation and lymph node enlargement of hila or mediastinum (P<0.05), but not with the tumor size, vacuole sign, vessel convergence sign and pleural indentation in CT images of peripheral NSCLC (P＞0.05). The expression of CD44v3 and CD44v6 correlated with the three-year survival rate in peripheral NSCLC patients (P<0.05). Conclusion Deep lobulation, spinous protuberant sign, short spiculation and lymph node enlargement of hila or mediastinum may indicate strong potential invasion and metastasis in peripheral NSCLC. The positive expression of CD44v3 and CD44v6 may suggest shorter survival time and poorer prognosis in peripheral NSCLC patients.

Objective: To investigate the effects of exercise combined with dietary intervention on glucose and lipid metabolism, liver and kidney function and cardiovascular function in obese adolescents. Methods: Seventy-one obese adolescents were enrolled in this study. Six weeks of exercise combined with dietary intervention were performed to determine anthropometry, glucose and lipid metabolism, liver and kidney function and cardiovascular function before and after intervention. Results: After 6 weeks of comprehensive intervention, in addition to significant improvement in anthropometry indicators, fasting insulin [(18.76±11.46 vs 11.32±6.54)uU/L], LDL-C [(2.96±0.69 vs 2.22±0.62)mmol/L], TG [(1.57±0.82 vs 0.89±0.37)mmol/L] and TC [(4.52±0.76 vs 3.53±0.62)mmol/L] decreased significantly and insulin resistance improved significantly. AST [(36.1±32.28 vs 22.89±7.27)U/L], ALT [(57.42±61.25 vs 27.86±22.12)U/L], blood urea nitrogen [(4.78±0.89 vs 3.44±0.79)mmol/L] and uric acid [(498.83±120.6 vs 471.07±120.96)mmol/L] were significantly decreased and the detection rates of fatty liver and kidney were significantly decreased(P<0.05), RHI(1.34±0.28 vs 1.69±0.45) and nitric oxide/ endothelin-1 (ET-1) (1.69±0.41 vs 2.67±0.86) significantly increased and atherogenic index (3.06±0.96 vs 2.58±0.80) significantly decreased(P<0.01). Conclusion The comprehensive intervention of exercise combined with diet can effectively improve anthropometry indicators, glycolipid metabolism, liver and kidney function as well as cardiovascular function of obese adolescents.

Facial paralysis is a frequently-occurring disease, which causes the loss of the voluntary muscles on one side of the face due to the damages the facial nerve and results in an inability to close the eye and leads to dropping of the angle of the mouth. There have been few objective methods to quantitatively diagnose it and assess this disease for clinically treating the patients so far. The skin temperature distribution of a healthy human body exhibits a contralateral symmetry. Facial paralysis usually causes an alteration of the temperature distribution of body with the disease. This paper presents the use of the histogram distance of bilateral local binary pattern (LBP) in the facial infrared thermography to measure the asymmetry degree of facial temperature distribution for objective assessing the severity of facial paralysis. Using this new method, we performed a controlled trial to assess the facial nerve function of the healthy subjects and the patients with Bell's palsy respectively. The results showed that the mean sensitivity and specificity of this method are 0.86 and 0.89 respectively. The correlation coefficient between the asymmetry degree of facial temperature distribution and the severity of facial paralysis is an average of 0.657. Therefore, the histogram distance of local binary pattern in the facial infrared thermography is an efficient clinical indicator with respect to the diagnosis and assessment of facial paralysis.
Facial Paralysis ; diagnosis ; physiopathology ; Humans ; Infrared Rays ; Pattern Recognition, Automated ; methods ; Skin Temperature ; Thermography ; instrumentation

Objective To provide reference for drug use in infant patients with Rhodococcus equi(R.equi) infection. Methods By participating in drug therapy for an infant patient with Rhodococcus equi infection,clinical pharmacist assisted physicians to develop treatment plan and provide reasonable pharmaceutical care. Results Therapeutic selection was improved through pharmaceutical care process. Conclusion Participation of clinical pharmacist can fully play their advantages in the field of drug efficacy,and improve the level of clinical treatment.

Fang's scalp acupuncture is mainly used in the treatment of nervous system diseases, bone and joint pain and mental diseases. It has significant effect on the early stage recovery stage and complications of post-stroke hemiplegia. The selection of acupoint is basically the same in improving patients with neurological impairment and dyskinesia, but differenct in the selection of acupoints in the treatment of complications. In recent years, it has been widely used in the treatment of children with attention deficit hyperactivity disorder and Tourette's syndrome. Fang's scalp acupuncture method was formed quite early with relatively complete theoretical system, but its clinical application is relatively insufficient. There exist some problems such as failing to reflect the theory of viscera and meridians in acupoint selection, the theory of acupoint selection by projection of cerebral cortex function, which needs to be proved further, the operation requirements are strict while the methods and practice of researchers are are differenct, and some clinical research design is not rigorous,etc.