Objective To explore microbic distribution feature of acute rhinitis pafienta' nasal cavity and dependabihty research of respiratory infection.Methods 436 acute rhinitis pafienta were randomly divided tO infection group(314)and non-infection group(122)depended on whether accompanying respiratory infection.Take blenna narium and carry out nasal cavity microbiological detection,meanwhile take blood and mesasure the content of serum IL-4 and IFN-γ.Results The staphylococcus aureus taked the most proportion(58.6%and 3 1.2%)of nasal cavity bacterial distribution in infection group and non-infection group,the following bacterium were the staphylococcus epidermidis(44.9%and 46.7%),bacillus meningitidis purulentae(7.9%and 4.9%)and bacillus coli(3.5%and 1.6%),the major eumycete were the peptostreptococcus asaeeharolyticus(1.9%and 1.6%),eubacterium lentum (1.6%and 0)and eubacterium mucus(0.9%and 0.8%),the major virus were the syncytial virus,the rhinoviru8es,adenodrus,influenza virus,parainfluenze virus and coronaviruses.The respiratory tract infection patients' content of serum IL-4 Were significant higher(P＜0.05)than the non-infection group,but the content ofIFN-γ were signifieanfly reduced(P＜0.05).Conclusion The staphylococcus aureus,syncytial virus,rhinoviruses,adenovirus and influenza virus have the close relation with the infection of the respiratory tract,which can cause the disorder of organism immune function.

To investigate the effects of genistein (Gen) on the biosynthesis of N-glycolylneuraminic acid (Neu5Gc) in rats, 80 4-week-old male SD rats were randomly equally into the control and genistein groups. The rats of control and genistein groups were fed 5% ethanol and 300 mg/(kg·d) genistein respectively by gavage. The contents of Neu5Gc in hind leg muscle, kidney and liver tissues of rats were measured by using high performance liquid chromatography coupled with fluorescence detector (HPLC/FLD), and the mechanism of inhibition of Neu5Gc synthesis was investigated by using the molecular docking of Gen and sialyltransferase. On the 15th day, the content of Neu5Gc in hind leg muscle and liver tissues decreased 13.77% and 15.45%, respectively, and there was no significant change in the content of Neu5Gc in kidney tissues. On the 30th day, the content of Neu5Gc in liver tissues decreased 13.35%, however, there was no significant change in the content of Neu5Gc in kidney tissues and Neu5Gc was not detected in hind leg muscle. The content of Neu5Gc in hind leg muscle, kidney and liver tissues decreased respectively 32.65%, 32.78%, 16.80% and 12.72%, 11.42%, 12.30% while rats fed on the 45th and the 60th days. Genistein has formed the hydrogen bond with sialyltransferase activity site residues His319, Ser151, Gly293, Thr328 and formed a hydrophobic interactions with the residues His302, His301, Trp300, Ser271, Phe292, Thr328, Ser325 and Ile274. The results of molecular docking indicated that the weak intermolecular interaction was the main cause of genistein inhibiting sialyltransferase activity. The research results provided an experimental basis for the subsequent reduction of Neu5Gc in red meat before slaughter.
Animals ; Gene Expression Regulation, Enzymologic ; drug effects ; Genistein ; pharmacology ; Male ; Molecular Docking Simulation ; Neuraminic Acids ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Transferases ; metabolism

Activation of the heart normally begins in the sinoatrial node (SAN). Electrical impulses spontaneously released by SAN pacemaker cells (SANPCs) trigger the contraction of the heart. However, the cellular nature of SANPCs remains controversial. Here, we report that SANPCs exhibit glutamatergic neuron-like properties. By comparing the single-cell transcriptome of SANPCs with that of cells from primary visual cortex in mouse, we found that SANPCs co-clustered with cortical neurons. Tissue and cellular imaging confirmed that SANPCs contained key elements of glutamatergic neurotransmitter system, expressing genes encoding glutamate synthesis pathway (Gls), ionotropic and metabotropic glutamate receptors (Grina, Gria3, Grm1 and Grm5), and glutamate transporters (Slc17a7). SANPCs highly expressed cell markers of glutamatergic neurons (Snap25 and Slc17a7), whereas Gad1, a marker of GABAergic neurons, was negative. Functional studies revealed that inhibition of glutamate receptors or transporters reduced spontaneous pacing frequency of isolated SAN tissues and spontaneous Ca