Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Genistein ; pharmacology ; Humans ; Nasopharyngeal Neoplasms ; pathology

OBJECTIVETo discuss the endoscopy-assisted surgery for deviated nose with the preoperative 3-D CT reconstruction.

METHODSSixty-six cases with deviated nose deformities underwent preoperative 3-D CT reconstruction. The deformities were showed in detail. The deviated nasal septum and deviated nose were corrected with the assistance of nasal endoscopy.

RESULTSThe patients were visited after operation at 1 week, 1 month, 3 months and 6 months. The nose shape and nose deviation were measured. Among the 66 cases, satisfactory result was achieved in 53 cases, good in 11 cases, unsatisfactory in 2 cases.

CONCLUSIONSPreoperative CT reconstruction is very help in understanding the deviated nose deformity. The rhinoplasty with nasal endoscopy has good exposure of the fracture deformity with less morbidity and side effect. The intraoperative time can be decreased also.

Adult ; Endoscopy ; Female ; Humans ; Male ; Middle Aged ; Nasal Septum ; abnormalities ; surgery ; Nose Deformities, Acquired ; surgery ; Rhinoplasty ; methods ; Treatment Outcome ; Young Adult

OBJECTIVE: To investigate the effects and mechanism of genistein on proliferation inhibition and onset of apoptosis in nasopharyngeal carcinoma cell line CNE. METHOD: Antiproliferative effect of genistein against CNE was tested by MTT method. Apoptotic phenotype of CNE was observed by electron microscope. Cell apoptosis percentage and cell cycle phase distribution of CNE were measured by flow cytometric assay. RESULT: A time dependent and dose dependent proliferation inhibition was demonstrated in CNE, the high potency has the obvious suppression on tumor growth function; Genistein induces nasopharyngeal cancer cell early apoptosis rate but to strengthen along with the density increase, 200 micromol/L group is highest, is 49.9%; and genistein induced a G2/ M cell cycle arrest. The characteristic morphological changes of apoptosis in CNE cells was observed after treated by genistein. CONCLUSION The time dependent and dose dependent proliferation inhibitory effect of genistein on nasopharyngeal cancer cells. Genistein's mechanism on suppressing effect is probably associated with inducing the cell apoptosis and intervening the cell circle of carcinoma cell. The results provide evidence for the potential usefulness of genistein in the prevention and treatment of human nasopharyngeal carcinoma.
Apoptosis ; drug effects ; Carcinoma ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Genistein ; pharmacology ; Humans ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; pathology