Objective To study the fingerprint determination method of Pogostemon cablin by HPLC.Methods A RP-HPLC method was established with the mixed solution of methnol and 0.1 % acetic acid aqueous solution as mobile phase(gradient elution),flow rate at 1.0 mL/min,detection wavelength at 254 nm,analytic time being 50 min and column temperature at 25 ℃.Results The results of comparative analysis of 10 batches samples showed that 15 common peaks were shared by Pogostemon cablin.The common peaks were classified to two regions and the second was the main characteristic region which had an amount of characteristic peaks.All samples had the similar fingerprint features.Conclusion The method is accurate,repetitive and can be applied for the identification and quality control of Pogostemon cablin.

Objective To explore the effect of multimodal analgesia combined with preemptive analgesia on perioperative pain control and sleep quality in elderly patients with intertrochanteric fracture.Methods A total of 100 elderly patients with femoral intertrochanteric factures were selected as treatment group,and 100 patients were selected as control group.The treatment group was treated with multimodal analgesia and preemptive analgesia,while the control group was treated with the conventional analgesia.The VAS score and the amount of tramadol at preoperative 4 8 h,2 4 h and 2 4 h,3 d,1 week,2 weeks after operation were compared between the two groups.The sleep quality of patients during hospitalization was evaluated by the PSQI score.The length of first time move for exercise and postoperative hospital stay were compared between two groups,and the postoperative Harris hip score at 2 weeks,3 months after treatment and adverse reactions were compared.Results The treatment group had a lower VAS score at preoperative 48 h,24 h and 24 h,3 d,1 week,2 weeks after operation when compared with control group (P ＜ 0.05).Tramadol dose in the control group was significantly higher than the treatment group (P ＜ 0.05),the PSQI score was significantly higher than the treatment group (P ＜ 0.05),the length of postoperative move for exercise was significantly longer than the treatment group (P ＜ 0.05).Harris hip score at 2 weeks after operation in the treatment group was significantly higher than that in control group (P ＜ 0.05),while 3 months after operation,the Harris hip score was similar between the two groups (P ＞ 0.05).The total incidence rate of adverse reactions were similar between the two groups (P ＞ 0.05).Conclusion Application of multimodal analgesia combined with preemptive analgesia is effective and safe in treatment of elderly patients with femoral intertrochanteric fracture,it can improve the sleep quality,promote the hip joint function recovery of patients in early stage.

Objective To explore the effect of sufentanil on preventing early pain afteRanesthesia surgery.Methods A total of 295 patients with colon canceRsurgery were randomly divided into sufentanil group and control group.The MAP,HR,SPO2 and restless score at different time points were compared between two groups.The pain score,sedation score and VAS score were compared between the two groups.The complications were compared.Results The MAP and HRat T1 in the sufentanil group were significantly higheRthan those in the control group (P<0.05).The first time pain score in the sufentanil group was significantly loweRthan the control group (P<0.05).The VAS scores of sufentanil group at T1,T2,T3 were significantly loweRthan the control group (P<0.05).The incidence rate of postoperative complications in sufentanil group was significantly loweRthan the control group (P<0.05).Conclusion Sufentanil is effective in preventing early pain afteRsurgery.

Objective To explore the effect of multimodal analgesia combined with preemptive analgesia on perioperative pain control and sleep quality in elderly patients with intertrochanteric fracture.Methods A total of 100 elderly patients with femoral intertrochanteric factures were selected as treatment group,and 100 patients were selected as control group.The treatment group was treated with multimodal analgesia and preemptive analgesia,while the control group was treated with the conventional analgesia.The VAS score and the amount of tramadol at preoperative 4 8 h,2 4 h and 2 4 h,3 d,1 week,2 weeks after operation were compared between the two groups.The sleep quality of patients during hospitalization was evaluated by the PSQI score.The length of first time move for exercise and postoperative hospital stay were compared between two groups,and the postoperative Harris hip score at 2 weeks,3 months after treatment and adverse reactions were compared.Results The treatment group had a lower VAS score at preoperative 48 h,24 h and 24 h,3 d,1 week,2 weeks after operation when compared with control group (P ＜ 0.05).Tramadol dose in the control group was significantly higher than the treatment group (P ＜ 0.05),the PSQI score was significantly higher than the treatment group (P ＜ 0.05),the length of postoperative move for exercise was significantly longer than the treatment group (P ＜ 0.05).Harris hip score at 2 weeks after operation in the treatment group was significantly higher than that in control group (P ＜ 0.05),while 3 months after operation,the Harris hip score was similar between the two groups (P ＞ 0.05).The total incidence rate of adverse reactions were similar between the two groups (P ＞ 0.05).Conclusion Application of multimodal analgesia combined with preemptive analgesia is effective and safe in treatment of elderly patients with femoral intertrochanteric fracture,it can improve the sleep quality,promote the hip joint function recovery of patients in early stage.

Objective To explore the effect of sufentanil on preventing early pain afteRanesthesia surgery.Methods A total of 295 patients with colon canceRsurgery were randomly divided into sufentanil group and control group.The MAP,HR,SPO2 and restless score at different time points were compared between two groups.The pain score,sedation score and VAS score were compared between the two groups.The complications were compared.Results The MAP and HRat T1 in the sufentanil group were significantly higheRthan those in the control group (P<0.05).The first time pain score in the sufentanil group was significantly loweRthan the control group (P<0.05).The VAS scores of sufentanil group at T1,T2,T3 were significantly loweRthan the control group (P<0.05).The incidence rate of postoperative complications in sufentanil group was significantly loweRthan the control group (P<0.05).Conclusion Sufentanil is effective in preventing early pain afteRsurgery.

OBJECTIVE： To establish a method for content determination of N-（E）-p-coumaroyltyrosine in leaves of Abrus cantoniensis， and to optimize its purification technology. METHODS： HPLC method was adopted for the content determination of N-（E）-p-coumaroyltyrosine in A. cantoniensis. The determination was performed on Hypersil BDS C18 column with mobile phase consisted of 0.1％ formic acid water （A）-methanol （B） （gradient elution） at a flow rate of 1.0 mL/min. The column temperature was set at 25 ℃. The detection wavelength was set at 300 nm， and sample size was 10 μL. Using polyamide resin as material， the yield of N-（E）-p-coumaroyltyrosine as indicators， single factor test was used to optimize the purification technology of N-（E）-p-coumaroyltyrosine， such as concentration， sample size， stationary adsorption time. RESULTS： The linear range was 2.575-51.50 μg （r＝0.999 9） for N-（E）-p-coumaroyl-tyrosine. The limit of quantification was 0.000 618 μg， and the detection limit was 0.000 129 μg. RSDs of precision， stability and reproducibility tests were all lower than 3％. The recoveries were 97.04％-102.43％ （RSD＝2.06％， n＝6）. The optimal purification technology was as follows： the concentration of the sample solution was 0.04 g /mL （by the leaves of A. cantoniensis）； sample capacity 50 mL； the sample flow rate was 1.0 mL/min； the stationary adsorption time was 20 min； the eluting solvents were ammonia containing water （containing 0.1％ acetic acid）， 20％ ethanol （containing 0.1％ acetic acid） and ammonia（pH 10）. Average yield was 98.94％，average dry paste content was 61.17 mg/g， and average dry paste purity was 19.73％ by optimal purification technology. CONCLUSIONS： Established method is simple， accurate and stable. The optimized technology is stable and feasible.