Objective To observe the effect of Chinese herbs most used in rheumatoid arthritis such as diaphoretics (ephedra), wind-expelling herbs (monkshood), blood activating drugs (angelica) and compound mixture in treating adjuvant arthritis, and study the compatibility law. Methods AA rats in groups were treated with ephedra-cinnamon twig mixture, monkshood-aconitum mixture, angelicachuanxiong mixture, herbs compound mixture, low dose herbs compound mixture which is composed of above-mentioned herbs and semblable function herbs. Compare with normal group, model group, tripterygium wilfordii hook (TWH) group, TNF-? and PGE2 level of hindpaw were measured by ELISA and spectrophotometer. Weight index of immune organs were measured to study the effect of different functional ingredient groups and complex on immune function. Result The hinderpaw PGE2 level and TNF-? level of model group was higher than normal group (P

Ice microalgae, which inhabit in the extreme conditions of polar marine environment, not only play an important role in Antarctica ecosystem but also show broad prospects in basic research and application. Ice microalgae have the specific physiological and metabolic properties that are responsible for their adaptation and acclimation to the special polar conditions. Based on the references in the recent twenty years and the partial research results of author's, the ice microalgae composition and distribution, and the application in bait, unsaturated fatty acid, anti-freezing proteins, UV-absorbing pigments and other active substances are reviewed. It is expected to provide references for the research and active substance exploitations of ice microalgae.

Objective:To probe the expression of miR-126 and VEGF in breast cancer, and the anti-tumor effect of miR-126. Methods:The expression of miR-126 and VEGF in breast cancer tissues and cells were detected by qRT-PCR and Western blot;after transfection with miR-126 mimics into MDA-MB-231,expression of VEGF was detected again,MTT assay and cell scratch test were used to verify the influence of miR-126 on proliferation and migration of tumor cells. Results: The expression of miR-126 was lower in the breast cancer tissues and cells,the expression of VEGF was negative correlation with it,increasing the expression of miR-126 may decrease the expression of VEGF and inhibit the proliferation and migration of breast cancer cells. Conclusion:miR-126 can reduce the proliferation and migration of breast cancer cells by inhibiting the expression of VEGF,which play an anti-tumor effect.

Objective To assess the efficiency and safety of mycophenolate mofetil (MMF) in patients with multiple sclerosis (MS) and neuromyelitis optica (NMO). Methods Twenty-seven patients with MS or NMO were selected, and the patients were divided into 2 groups:MMF group (MMF combined with glucocorticoid treatment group, 10 cases) and glucocorticoid group (only glucocorticoid treatment group, 17 cases). There were 5 cases with MS and 5 cases with NMO in MMF group. There were 13 cases with MS and 4 cases with NMO in glucocorticoid group. The therapeutic efficacy 6 months after treatment, expanded disability status scale (EDSS) before treatment and 6 months after treatment, and annualized relapse rate (ARR) were compared; and the safety was observed. Results There was no statistical difference in efficacy rate 6 months after treatment between MMF group and glucocorticoid group: 9/10 vs. 11/17, P>0.05. The EDSS scores 6 months after treatment in MMF group and glucocorticoid group were significantly lower than those before treatment: (2.41 ± 2.05) scores vs. (3.40 ± 2.05) scores and (1.17 ± 0.92) scores vs. (2.38 ± 1.28) scores, and there were statistical differences (P<0.05), particularly for the patients with MS. The ARR 6 months after treatment in MMF group was significantly lower than that before treatment: 0 time/year vs. 0.75 times/year, and there was statistical difference (P<0.05). The difference of ARR before and after treatment in MMF group was significantly higher than that in glucocorticoid group: 0.75 times/year vs.- 0.46 times/year, and there was statistical difference (P<0.01), particularly for the patients with MS. Only 1 female patient had myalgia when taking higher dosage of MMF, and the symptom tended to relieve after the dosage was reduced. Conclusions MMF is effective in the treatment of MS and NMO. MS can improve the neurological function and reduce the recurrence of the disease;and the safety is high.

Objective To compare the clinicopathological features of Borrmann type Ⅳ gastric cancer with other gastric cancer and explore prognostic factors of the patients with Borrmann type Ⅳ cancer.Methods We retrospectively reviewed the clinical data of 671 advanced gastric cancer patients.They were divided into 2 groups:Borrmann type Ⅳ (64 cases) and other macroscopic Borrmann types of cancer (607 cases).Their clinicopathologic characteristics and overall survival data were analyzed.Results Age,sex,tumor size,tumor location,lymph node metastasis,distant metastasis,TNM classification were discrepant between Borrmann type Ⅳ and other macroscopic Borrmann types of cancer.The 5-year survival rate of Borrmann type Ⅳ cancer patients was 20.1％,while it was 40.3％ for other types of cancer (P ＜ 0.05).The 5-year survival rate for Borrmann type Ⅳ gastric cancer and the other type gastric cancer was 50.0％ and 72.0％ at stage Ⅰ,30.0％ and 57.9％ at stage Ⅱ,18.0％ and 28.4％ at stage Ⅲ,and 16.4％ and 20.0％ at stage Ⅳ (all P ＜ 0.05),respectively.Multivariate analyses revealed age,histology differentiation type,tumor size,the Borrmann type carcinoma and tumor stage to be independent prognostic factors for survival.Conclusions Borrmann type carcinoma has unique clinicopathological features compared with other types of gastric carcinoma and is an important independent prognostic factor.

Objective To evaluate negative lymph node count (NLNC) in prediction of prognosis of T3 gastric cancer after radical resection.Method 214 T3 patients of radical gastrectomy with complete clinical and follow-up data between Jan 2003 to Dec 2007 were enrolled.Survival was determined by the Kaplan-Metier method and univariate analysis was done by Log-rank test,Multivariate analysis was performed using the COX proportional hazard regression model.-2loglikelihood value and the hazard ratio (HR) value were used to compared the value of number of lymph node-negative (NLNC) staging and pN staging and lymph node metastasis rate (MLR) in gastric cancer prognosis evaluation.Results Univariate analysis showed that,pN stage (x2 =31.664),MLR stage (x2 =34.123),tumor size (x2 =5.025),type of differentiation (x2 =5.993),Borrmann classification (x2 =5.401),NLNC stage (x2 =37.256) were related to survival (P ＜ 0.05).COX multivariate analysis showed that-2loglikelihood of pN staging is 1 336.761,HR value is 1.464,-2loglikelihood value of MRL staging is 1 335.821,HR value is 1.441.-2loglikelihood value of NLNC staging is 1 326.902,HR value is 1.725.The N0 and N1 staging prognosis in different NLNC staging was significant (P =0.008,P =0.014).Conclusions Sufficient number of negative lymph node prolongs survival and reduces the risk of early recurrence in advanced gastric cancer.

Background Staphylococcus aureus,Staphylococcus epidermidis and Pseudomonas aeruginosa are common pathogenic bacteria in ophthalmology.To seek an optimal antimicrobial method is critical for the prevention of infection.Objective This study was to investigate the antimicrobial efficacy of riboflavin-ultraviolet A (UVA) on Staphylococcus aureus,Staphylococcus epidermidis and Pseudomonas aeruginosa.Methods Ten strains of frozen Staphylococcus aureus,Staphylococcus epidermidis and Pseudomonas aeruginosa were incubated to culture dishes and resuscitated for 2 times.The bacteria were put into bacterial dilution to prepare the suspension of 0.5 Mclntosh concentration and then the suspension was diluted 10-folds.UVA was used to irradiate the culture dishes added 0,0.1％,0.5％ riboflavin for 10,20,30 minutes with the exposure intensities of 1,2,3 mW/cm2,and the optimal bacteriostatic condition was evaluated by measuring the diameters of inhibition zone.Staphylococcus aureus,Staphylococcus epidermidis and Pseudomonas aeruginosa were divided into normal control group,riboflavin group,UVA group and photodynamic therapy (PDT) group (riboflavin+UVA),and colony forming unit was compared among the groups.Results The optional experimental conditions of PDT inactivating microorganisms were determined as UVA wavelength 370 nm,UVA exposure rate 3 mW/cn2,UVA exposure time 30 minutes and riboflavin 0.1％.The colony forming units were decreased in turn in the normal control group,riboflavin group,UVA group and PDT group (F =110.990,190.841,118.197,all at P =0.000).Compared with the normal control group,the colony forming units were significantly decreased in the UVA group (t =14.640,7.550,5.320,all at P＜0.05),and the colony forming units were significantly decreased in the PDT group (t =25.480,14.750,12.920,all at P＜0.05).The colony forming units decreased by 46.4％-50.2％ in the UVA group and 91.6％-96.7％ in the PDT group.There was no significant difference in colony forming units between the normal control group and the riboflavin group(t =0.660,0.470,0.510,all at P＞0.05).Conclusions PDT can effectively inactivate common bacteria in ophthalmology.This result suggests that PDT has a potential in the treatment of microbial keratitis.

OBJECTIVE:To establish an optimal therapeutic window concentration of CsA trough levels in renal transplant recipients on triple immunosuppressants regimen METHODS:A total 1 874 samples from 268 renal transplant recipients were measured by fluorescence polarization immunoassay(FPIA) According to the duration after operation and clinical diagnoses ,the whole blood CsA trough levels were compared among subgroups RESULTS:The optimal therapeutic window concentration of CsA was 300～400?g/L(within 1 month after operation),250～350?g/L(2nd～3rd month),150～250?g/L(4th～6th month),100～200?g/L(7th～12th month)and 100～150?g/L(more than 12 months) CONCLUSION:The above mentioned therapeutic window concentratin of CsA trough levels was ideal for renal transplant recipients with no marked acute toxic effects and rejection reaction

With the development of social economy, people’s eating habits and lifestyle are gradually changing, resulting in a higher and higher incidence of obesity. Obesity related glomerulopathology, as a chronic complication, has attracted more and more attention. The mechanism of the kidney injury induced by obesity is complex, and there is no reliable treatment at present, so it has become a hot spot in basic research and a difficult point in clinical work. In recent years, the bariatric metabolic surgery has benefited many severely obese patients and achieved good weight loss results. This paper will sum up the main types and advantages of metabolic surgery, summarize the role and mechanism of metabolic surgery in reducing proteinuria and improving renal function, and look forward to its future development direction.