1.The comparison of specific antibodies levels in the BALB/c mice immunized with the pcDNA3.1 recombinant plasmids of E gene partial sequence from different Dengue type 2 virus strains
Jiao WANG ; Li ZUO ; Cong WANG ; Xuezheng LI
Chinese Journal of Microbiology and Immunology 2009;29(3):229-233
Objective To compare humoral immune response of BALB/c mice immunized by recombinant plasmids poDNA3.1-BE and pcDNA3.1-NE. Methods The BALB/c mice were immunized by recombinant plasmids pcDNA3.1-BE, pcDNA3.1-NE with adjuvant. Each animal received a primary inoculation and two boosts at 2-week intervals. Then the blood samples of BALB/c mice were collected from different experiment groups at day 14, 28, 42, 70 and 98, respectively after first immunization. The specific IgM/IgG antibodies for E protein in serum were confirmed by indirect ELISA. And then the activities of the specific neutralizing antibody were determined by cytopathic effect inhibition (CPEI). Results The levels of specific IgM/IgG antibodies and neutralizing antibody activities were increased in PODNA3.1-BE enhance immunization group than that of other groups. The result had been observed longer duration of antibody level in pcDNA3.,-BE enhance immunization group. Conclusion Humoral immune response were significant differences between recombinant plasmid pcDNA3.1-BE and PcDNA3.1-NE immunized mice groups.
2.The impact of modified Liangge powder on platelet activation markers and release of proinflammatory cytokine of mice by stimulation of lipopolysaccharide
Jingshu ZHANG ; Yongqiang WANG ; Bing WANG ; Xuezheng LIU
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2015;26(2):133-137
Objective To observe the impact of modified Liangge powder (MLP) on platelet activation markers and the release of proinflammatory cytokine in mice by stimulation of lipopolysaccharide (LPS). Methods 112 male mice were randomly divided into control group, model group and MLP low, middle and high dose treatment groups. The sepsis model was reproduced by injection of LPS 10 mg/kg into a mouse tail vein. In the control group, normal saline 10 mg/kg was injected into the tail vein of mouse. The MLP low, middle, and high dose groups received 0.94, 1.89, 2.84 g/mL MLP 0.02 mL/g by gavage respectively for 3 days, while the control group and model group received equal amount of normal saline by gavage for 3 days. After modeling for 24 hours and 72 hours, 8 mice in each of the three different dose MLP groups and model group were killed and their blood was taken. In the control group, after modeling for 24 hours, 8 mice were killed and their blood was taken. Platelet (PLT) was counted by blood cell analyzer, plasma interleukin-10 (IL-10), high mobility group protein B1 (HMGB1) and platelet factor 4 (PF4) were detected by enzyme-linked immunosorbent assay (ELISA). In each group after modeling for 72 hours, another 8 mice were taken, and laser scanning confocal microscopy was used to measure the platelet cytosolic Ca2+ concentration. Results Compared with the control group, the level of PLT at 24 hours(×109/L: 347.70±115.10 vs. 1 013.10±136.60) was decreased, and the levels of IL-10 (μg/L: 356.86±34.72 vs. 39.50±23.45), HMGB1 (mg/L: 16.24±4.49 vs. 10.75±1.91), PF4 (μg/L: 5.43±0.61 vs. 1.33±0.40) and Ca2+ (nmoL/L: 8.60±0.52 vs. 1.05±0.33) were elevated in model group. Compared with the model group, the levels of PLT in the MLP high, middle and low dose groups were all significantly elevated; the increase in PLT in middle dose group after modeling for 72 hours was the most remarkable (×109/L:952.13±104.02 vs. 771.50±129.30, P < 0.05); the levels of IL-10, HMGB1, PF4, Ca2+ in MLP low, middle, high dose groups were significantly decreased. The most obvious degree of decrease in level of the following indexes were as follows:IL-10 in MLP high dose group at 72 hours after modeling (μg/L:110.17±29.12 vs. 441.50±30.72), HMGB1 in MLP high dose group after modeling for 24 hours (mg/L: 10.33±3.52 vs. 16.24±4.49), PF4 in MLP middle dose group after modeling for 24 hours (μg/L:2.08±0.92 vs. 5.43±0.61) and Ca2+ in MLP high dose group (nmoL/L:2.97±0.96 vs. 8.60±0.52, all P<0.05). Conclusion MLP may possibly down-regulate the inflammatory cytokines release induced by LPS to inhibit the activation of platelet Ca2+, in turn prevent the activation of platelet and improve thrombocytopenia caused by LPS.
3.Comparison of efficacy and economic efficiency of neuroendoscope versus microscope for intracerebral hemorrhage
Xuezheng FAN ; Jinneng HUANG ; Shouhong ZHU ; Shuangui HU ; Boqiang WANG
The Journal of Practical Medicine 2016;32(18):3007-3010
Objective To investigate the differences of efficacy and economic efficiency between neuroendoscope and microscope for intracerebral hematomas cleaning operation. Methods From August 2014 to August 2015, clinical data of 68 cases with intracerebral hemorrhage were analyzed retrospectively. Hematoma evacuation was carried out by microscope in 37 cases (microscope group) received and 31 cases (endoscope group) underwent neuroendoscope. Following parameters were compared: the operation related index (hematoma clearance rate , intraoperative blood loss , the operation time , skin incision , bone window size ) , Glasgow Outcome Scale at 6 months after the operation, complications (intracranial infection, lung infections, gastrointestinal bleeding), medical economic parameters (the postoperative hospital stay, drug cost, cost other than drug, and total hospital cost). Results The hematoma clearance rate, Glasgow Outcome Scale at 6 months after the operation in the endoscope group were significantly better than those in the microscope group (P <0.05). The intraoperative blood loss, the operation time, skin incision, bone window size the postoperative hospital stay , drug costs , no drug costs , and total hospital cost in the neuroendoscope group were significantly lower than those in the microscope group (P < 0.01). The intracranial infection, lung infections in the neuroendoscope group were lower those in the microscope group (P < 0.05). There was no statistical difference in the incidence of gastrointestinal bleeding between the two groups (P > 0.05). The postoperative hospital stay, drug costs , no drug costs , and total hospital cost were significantly less in neuroendoscope group than that in microscope group (P< 0.01). Conclusion Neuroendoscopy has less trauma,there are some advantages of neuroendoscopy for intracerebral hematomas cleaning operation , such as minimal trauma , prominent effect , good prognosis and low cost. It should be widely applied in clinical practice.
4.Effect of Berberine on AQP4 Expression in Focal Cerebral Ischemia Area in Diabetic Rats
Hongyu CUI ; Xinyu LIANG ; Yijun LU ; Peng WANG ; Xuezheng LIU
Journal of China Medical University 2015;(12):1102-1105
Objective to investigate the effect of berberine on the expression of AQP4 and neuronal injury after focal cerebral ischemia in diabetic rats. Methods Male SD rats were randomly divided into four groups:control(sham surgery),middle cerebral artery occlusion and reperfusion (MCAO/R),MCAO/R treated with vehicle(DMSO),MCAO/R treated with berberine. the transient focal ischemia/reperfusion was induced by in-troducing silicone-coated monofilament nylon suture from the right external carotid artery into the origin of the middle cerebral artery,which was re-moved after 60 min. In group treated with berberine,the rats were injected with berberine before and after suffered from cerebral ischemia. Similarly, in group vehicle,the animals received DMSO vehicle at the same time. the score of neurological behavior was evaluated 24 h after reperfusion. Mean-while,the rats were sacrificed for Nissl staining. to estimate cerebral edema,the wet-dry ratio was measured. the expression of AQP4 in the border of the infarct region in different groups was observed by Western blot. Results Compared with the model group,berberine improved neurological deficits(P < 0.05). Berberine treatment inhibited the neuronal deformation shown by Nissl staining(P < 0.05). Berberine significantly decreased the wet-dry ratio and reduced the expression of AQP4(P < 0.05). Conclusion these results suggested that berberine could induce neuroprotection against ischemic injury by inhibiting the expression of AQP4 in diabetic rats.
5.Adherence management to health belief model based-antiretroviral therapy
Xuezheng JIN ; Weihua CAO ; Xinlun WANG ; Yubo LI
Chinese Journal of Health Management 2013;(3):171-173
Objective To describe the status of adherence to antiretroviral therapy (ART) in low endemic area,and to explore the factors affecting ART adherence so as to provide evidence for behavior management program.Methods A cross-sectional study was conducted in 53 patients receiving free ART in Chuanying and Yongji of Jilin Province.Structured face-to-face interview was carried out to determine sociodemographic characteristics,medical treatment information,medication adherence behaviors,health belief and self-efficacy,doctor-patient relationships and health service information.Results Among 53 patients,3 reported drug discontinuance.Of the other 50 patients,41 (82.0%) obeyed the request of the doctors (to be defined as adherence).All the participants had high levels of perceived benefits of adherence,perceived severity of non-adherence and self-efficacy.94.3% of them reported using medication reminders,88.7% reported receiving directly observed therapy (DOT),and 73.6% reported falling into the habit of drug administration.Conclusions HIV/AIDS patients show relatively good adherence to medical treatment.Local comprehensive education and supportive programs may contribute to patients' good adherence to ART.
6.Associations of serum uric acid, bilirubin levels and short-term outcome in patients with acute ischemic stroke
Dandan LU ; Lan HOU ; Shuyan WEI ; Li WAN ; Xuan LI ; Xuezheng LU ; Jing WANG ; Pei WANG
International Journal of Cerebrovascular Diseases 2016;24(3):193-197
Objective To investigate the associations of baseline serum uric acid, bilirubin levels with short-term outcome in patients with acute ischemic stroke. Methods The clinical data in successive patients with acute ischemic stroke were colected, including the serum levels of uric acid and bilirubin on admission, the National Institutes of Health Stroke Scale (NIHSS) score, and the modified Rankin scale (mRS) score at discharge or at day14 (mRS 0-2 was defined as good outcome, > 2 was defined as poor outcome). Results A total of 162 patients with ischemic stroke were enroled, including 114 in the good outcome group and 48 in the poor outcome group. There were significant differences in proportions of the patients with diabetes melitus (51. 75% vs. 75. 00% ; χ2 = 7. 526, P = 0. 006), previous history of stroke or transient ischemic attack (TIA) (18. 42% vs. 50. 00% ; χ2 = 17. 790, P = 0. 001), as wel as the baseline diastolic blood pressure (87. 061 ± 12. 245 mmHg vs. 82. 375 ± 10. 949 mmHg; t = 2. 293, P = 0. 023; 1 mmHg =0. 133 kPa), high-density lipoprotein cholesterol (1. 604 ± 0. 299 mmol/L vs. 1. 265 ± 0. 206 mmol/L; t =3. 227, P = 0. 002), fasting glucose (2. 875 ± 0. 438 mmol/L vs. 8. 160 ± 0. 592 mmol/L; t = - 4. 761, P <0. 001), uric acid (289. 365 ± 77. 168 μmol/L vs. 248. 206 ± 66. 206 μmol/L; t = 3. 111, P = 0. 002), total bilirubin (14. 673 ± 2. 213 μmol/L vs. 10. 395 ± 2. 714 μmol/L; t = 3. 779, P = 0. 001 ), direct bilirubin (6. 036 ± 1. 392 μmol/L vs. 4. 956 ± 1. 379 μmol/L; t = 2. 088, P = 0. 038), and indirect bilirubin (8. 634 ± 2. 307 μmol/L vs. 5. 439 ± 1. 223 μmol/L; t = 4. 219, P < 0. 001) levels between the 2 groups. Multivariate logistic regression analysis showed that the previous history of stroke or TIA (odds ratio [ OR ] 3. 751, 95% confidence interval [CI ] 1. 395-10. 091; P = 0. 009) and baseline NIHSS score (OR 2. 723, 95% CI 1. 093-6. 783; P = 0. 031) were the independent risk factors for poor outcome of ischemic stroke; while uric acid (OR 0. 357, 95% CI 0. 141-0. 900; P = 0. 029), high-density lipoprotein (OR 0. 262, 95% CI 0. 079-0. 870; P = 0. 029), and indirect bilirubin (OR 0. 117, 95% CI 0. 025-0. 539; P = 0. 006) were independently correlated with good outcome. Conclusions The increased baseline uric acid and indirect bilirubin levels are the favorable factors for good outcome in patients with acute ischemic stroke.
7.Role of FTY720 therapy in motor nerve function recovery after acute spinal cord injury in rats
Jianmin WANG ; Kai TANG ; Benchen XU ; Chengke WANG ; Qazi BASIT ; Fang LI ; Xuezheng ZHAO
Chinese Journal of Trauma 2013;29(10):1000-1007
Objective To investigate the effect and mechanism of FTY720 in protecting the motor nerve function in rats with acute spinal cord injury (ASCI).Methods A model of ASCI in the rat was established using Allen' s method.The rats were divided into five groups according to random number table:Group A (normal control group,n =6),Group B (sham group,n =27),Group C (ASCI group,n =27),Group D (FTY720 systemic treatment group,n =27) and Group E (FTY720 topical treatment group,n =27).After treatment,each group was observed for the changes of lymphocytes in peripheral blood and topical spinal cord tissue,BBB score,pathological changes of spinal cord tissue and expression intensity of glial fibrillary acidic protein (GFAP).Results After ASCI in rats,the infiltration of local T lymphocyte subpopulations CD3,CD4 and CD8 was promoted,but the number of peripheral blood T lymphocyte subpopulations CD3,CD4 and CD8 was decreased.After FTY720 therapy,both local and peripheral blood lymphocytes were reduced in number and the decrease of peripheral blood lymphocytes in Group E was between Groups C and D.BBB score,pathologic observation and GFAP staining showed a neuroprotective effect of FTY720 and better motor nerve function recovery in Group E than in Groups C and D.Conclusions FTY720 therapy facilitates motor nerve function recovery.Local FTY720 application reduces peripheral blood lymphocytes and lowers the incidence of infection.
8.Association of metabolic syndrome and chronic kidney diseases in Zhengzhou adults
Dan GAO ; Zhangsuo LIU ; Dongwei LIU ; Xiaozhou HU ; Pei WANG ; Xuezheng SHI
Chinese Journal of Endocrinology and Metabolism 2009;25(4):414-415
ortant risk factor for chronic kidney diseases.
9.Effects of inhibiting NgR on retinal ganglion cells density and synaptophysin expression of diabetic rats
Wenqiang LIU ; Yubo WANG ; Huimin LIANG ; Zhaowei LI ; Zheng LI ; Xuezheng LIU
Recent Advances in Ophthalmology 2017;37(2):106-109
Objective To investigate the effects of inhibiting NgR on retinal ganglion cells density and synaptophysin expression of diabetic rat.Methods Thirty-two SD male rats were randomly divided into normal control group,diabetic group,siNgR group and scNgR group,8 rats in each group.Normal control group was given no any treatment.Diabetes model was induced by intraperitoneal injection of 50 mg · kg-1 streptozotocin in diabetic group,siNgR group and scNgR group,and the blood giucose more than 16.7 mmol · L-1 at 72 hours was set as the successfully model.The rats of siNgR group were intravitreally administrated with anti-NgR nucleotide and the rats of scNgR group intravitreally administrated with negative nucleotide.Eight weeks later,HE staining was conducted to detect density of retinal ganglion cell (RGC),immunofluorescence was used to evaluate the expression and distribution of synaptophysin (a marker of synaptic number).Relative expression of NgR and synaptophysin in retina were analyzed by Western blot.Results RGC density in normal control group,diabetes group,siNgR group and scNgR group were (624.33 ± 3.51) mm-2,(420.00 ± 2.65) mm-2,(621.67 ± 1.53) mm-2,(416.67 ± 2.52) mm-2,respectively.There was significant difference among four groups (F =5985.37,P < 0.01).Compared with normal control group,RGC density in diabetes group and scNgR group were obviously decreased (all P <0.01),but siNgR group had no obviously change (P > 0.05).The synaptophysin mainly expressed in the inner and outer network layer.Compared with normal control group,the positive expression of synaptophysin in diabetes group and scNgR group were decreased,but siNgR group had no obviously change.The relative expression of NgR in normal control group,diabetes group,siNgR group and scNgR group were (11.26 ±0.02) %,(19.38 ± 0.10) %,(11.17 ± 0.02) %,(19.47 ± 0.31) %,respectively.There was significant difference among four groups (F =2466.09,P < 0.01).Compared with normal control group,the relative expression of NgR in diabetes group and scNgR group were obviously decreased (all P < 0.01),but siNgR group had no obviously change (P >0.05).The relative expression of synaptophysin in normal control group,diabetes group,siNgR group and scNgR group were (35.76 ± 0.15) %,(25.47 ± 0.36) %,(35.28 ± 0.12) %,(25.03 ± 0.75) %,respectively.There was significant difference among four groups (F =583.70,P < 0.01).Compared with the normal control group,the expression of synaptophysin in diabetic group and scNgR group were decreased increased (all P < 0.01),while there was no significant difference in siNgR group (P > 0.05).Conclusion Inhibiting the expression of NgR in the retina of diabetic rats can help to restore the number of synapses and protect the damaged RGC.
10.Application of tissue synchronization imaging by real-time thriplane echocardiography in cardiac resynchronization therapy
Changhua WEI ; Yisa WANG ; Haohui ZHU ; Jiyun CHEN ; Fan GAO ; Xuezheng QU ; Jianjun YUAN
Chinese Journal of Ultrasonography 2015;(7):553-557
Objective To investigate the applicable value of tissue synchronization imaging (TSI)by real-time thriplane echocardiography (RT3PE)in cardiac resynchronization therapy (CRT).Methods Twenty-four patients with heart failure scheduled for CRT and 24 healthy controls were enrolled.Two dimensional echocardiography was performed to get the left ventricular internal diameter at end-diastole and end-systole(LVIDd,LVIDs ),left ventricular ejection fraction (LVEF ),velocity time integral of left ventricular outflow tract flow (VTILVOT ).The RT3PE TSI was performed to measure the time to peak systolic velocities(Tp)of left ventricle basal and middle segments(12 segments),software will automatically calculate the maximal difierence (12-Tp-DIF)and standard deviation (12-Tp-SD)of Tp in 12 segment. Identity the segment where wall motion most delay and to guide electrode placement.AV and VV interval optimization were performed under guidance of TSI after 1 month of CRT.Results In before CRT,after CRT,after optimization and control group,LVIDd,Tp,LVIDs,12-Tp-DIF and 12-Tp-SD were decreased in the order,LVEF and VTILVOT were increased in the order.There were significant difference between the groups(all P < 0.05 ).TSI showed that left ventricular systolic dyssynchrony was present in all patients before CRT.The most delay segment and electrode location was consistent.There were close correlation between 12-Tp-DIF and LVEF (r =-0.70,P =0.000),between 12-Tp-SD and LVEF(r =-0.73,P =0.000).Conclusions Left ventricular systolic synchronicity can be evaluated accurately and intuitively using TSI by RT3PE.It has important clinical significance in prospectively select patients for CRT,help to guide electrode placement and to optimize device programming.