Objective To study the relationship between the expression of MDR 1 gene in cochlea lateral wall and the accumulation of adriamycin in endolymph of guinea pig.Methods Twenty four white guinea pigs with red eyes were randomly chosen,and administered an intravenous injection of 30 mg/kg adriamycin or N.S.The animals were sacrificed 24 hours later,the cochlea lateral wall was then immediately removed after the endolymph was drawn by the siphon from the basal turn of cochlea. The expression of the MDR 1 mRNA in cochlea lateral wall of guinea pigs were observed by RT-PCR.The ADM concentration in endolymph was measured by high performance liquid chromatography.Results The accumulation of adriamycin in endolymph negatively correlated with the expression of MDR 1 gene in cochlea lateral wall(r s=-0.901, P

Objective To examine the expression and the significance of mdr 1 mRNA in guinea pig cochlea lateral wall at different developmental stages.Methods Thirty white guinea pigs with red eye including three developmental stages were used in this study. The expression of mdr 1mRNA in the cochlear lateral wall was measured in situ hybridization and analyzed by the softeware of MPLAS-500.Results mdr 1mRNA was detected mainly in the endothelial cells.The expression intensity of mdr 1mRNA was different in guinea pig cochlea lateral wall at different developmental stages, mdr 1mRNA in 1～2 weeks group was significantly lower than that of 3-week-group and 4-week-group(P0.05).Conclusion mdr 1mRNA is found in the normal cochlea lateral wall cells. The expression intensity is different with the development of guinea pig cochlea lateral wall.

Objective To create a method to assay adriamycin in endolymph of guinea pig cochlea,and provide methodology background to study the accumulation of ototoxicity substance in inner ear.Methods 12 normal guinea pig were killed quickly after 24 hours following adriamycin injection.The concentrate of adriamycin in endolymph of guinea pig cochlea was assayed by high performance liquid chromatography(HPLC).Results The linear relation of concentration of adriamycin in endolymph was well when the concentration was from 2.56 ng/mL to 1398.00 ng/mL.Correlation coefficient of linearity was 0.999 (n=10).The percentage of retrieve for adriamycin in endolymph was 96.3%.Relative standard deviation (RSD) was 2.61%.The lowest limitation was 1.9 ng/ml .Conclusion The sensibility of HPLC is high,and it can accurately detect the concentration of adriamycin in endolymph of guinea pig cochlea,especially the adriamycin is administrated in vein.

Objective To evaluate endoscopic minimally invasive surgery in the treatment of sphenoidal and ethmoidical mucoceles. Methods A total of 25 patients diagnosed as sinus mucoceles, 10 in the sphenoid sinus and 15 in the ethmoid sinus, were subjected in this study, who underwent endoscopic marsupialization in our department from 1998 to 2000. Postoperatively, the patients were followed from 1 to 3 years. Results All 25 sphenoidal and ethmoidical mucoceles were satisfactorily approached endoscopically, and operating cavities received satisfactory marsupialization. The symptoms were improved or relieved in 23 patients. None had significant complications. Conclusion Endoscopic minimally invasive surgery is a safe and valuable way in the treatment of sphenoidical and ethmoidical mucoceles.

Objective To evaluate the therapeutic effect of proximal femoral nail(PFN)and dynamic hip screw(DHS)for intertrochanteric femoral fracture(IFF).Methods From September,2004 to May,2007,39 IFF patients treated by PFN and 36 by DHS were enrolled into the analysis.The operative complications,postoperative complications and the recovery of hip function of IFF patients were observed to evaluate the therapeutic effect of the two methods.Results The blood loss amount was higher,the incidence of postoperative complications was higher and the excellent rate of Harris score of hip function was lower in IFF patients treated by DHS than that in IFF patients by PFN(P

Objective To investigate the protective effect of bicyclol tablets on drug-induced hepatic injury in patients with severe psoriasis.Methods One hundred and six patients with severe psoriasis and drug-induced hepatic injury were enrolled in this study,and randomly divided into 2 groups:bicyclol group (53 cases) treated with oral bicyclol tablets (25 mg thrice a day) for 8 weeks,diammonium glycyrrhizinate group (53 cases) treated with diammonium glycyrrhizinate capsules (150 mg thrice a day) for 8 weeks.Serum biochemical indices of hepatic function were measured before and after treatment,therapeutic efficacy was evaluated,and adverse reactions were observed and recorded after treatment.Intergroup and intragroup differences in these parameters were assessed.Results During the treatment,3 patients were lost to follow up.After treatment,serum biochemical indices of hepatic function markedly improved in both the bicyclol group (52 cases) and diammonium glycyrrhizinate group (51 cases) (both P ＜ 0.01),and the bicyclol group showed a better treatment response than the diammonium glycyrrhizinate group (P ＜ 0.01).The marked response rate was 71.15％ and 47.06％ (P ＜ 0.05),and the response rate was 84.62％ and 66.67％ (P ＜ 0.05),respectively,in the bicyclol group and diammonium glycyrrhizinate group.No adverse reactions related to tested drugs were observed in either of the two groups.Conclusion Bicyclol can attenuate antipsoriatic drug-induced hepatic injury with good safety.

A series of reforms on the teaching of medical chemistry experiment has been carried out to arouse students' interest in medical chemistry experiment study and improve the quality of instruction,which includes the content of course,the teaching method and the inspection method.Good results have been achieved in the aspect of development of students' comprehensive quality,which lays a good foundation for the study of other courses.

In order to improve teaching quality of medical organic chemistry,problem-based learning was introduced.The characteristic,implementary process and outlines of problem-based learning were discussed in combination with the characteristic of organic chemistry in medical university.

Objective To determine the serum soluble CD226/PTA1(sCD226/PTA1)level in pa-tients with psoriasis vulgaris.Methods Serum sCD226/PTA1level was measured by sandwich ELISA in30patients with psoriasis vulgaris before treatment(17patients in active stage and13in inactive stage),10patients after treatment,and15healthy individuals as a control.Results Serum sCD226/PTA1level was significantly increased in patients with psoriasis compared with that in healthy controls(P

Objective To observe the survival rate of free flaps and incidence of vascular crisis following the perforator flap transfer in end-to-side microvascular anastomosis compared with the traditional method and investigate the scientificity and practicability of this scheme.Methods From February, 2012 to October, 2013, 152 cases of free flaps (including superficial illiac circumflex perforator flap, lateral arm flap, peroneal perforator flap, and anterolateral thigh flap) were performed.The end-to-side anastomosis were performed in 72 cases while end-to-end anastomosis in 80 cases;All patients were followed up for 6-24 months.The occurrence of vascular crisis in all cases were observed and recorded.The results of the two groups were conducted statistical analyzed.Results The average anastomosis time for end-to-side anastomosis (21.3 ± 3.8 min) was significantly higher than the end-to-end anastomosis (14.4 ± 3.2 min) (P ＜ 0.05).The incidence of anastomotic vasospasm after end-to-side anastomosis (5.6％) was significantly lower than traditional method (16.3％) (P ＜ 0.05).There was no significant difference in the survival rate of the free flaps between the end-to-side anastomosis group (97.2％) and the traditional group postoperatively (96.3％) (P ＞ 0.05).Conclusion Although anastomosis times were increased in the end-to-side group, this technique showed lower spasm rate and similar flap survival rate.Therefore, this technique is a reliable and technically feasible method and should be considered first as a choice when facing vessel discrepancy and for preserving the recipient artery and vein system.