Objective To study the effects of traumatic optic neuropathy treated with optic canal decompression through transnasal endoscope.Methods The data of 10 cases of traumatic optic neuropathy treated with optic canal decompression by transnasal endoscope from April 2003 to February 2004 were retrospectively reviewed.Results In 10 cases,the visual acuity of six cases recovered,and four cases were not improved.Conclusion Optic canal decompression by transnasal endoscope and corticosteroids are beneficial in managing the optic neuropathy.

Bone Neoplasms ; pathology ; Chondrosarcoma ; pathology ; Head ; pathology ; Humans ; Neck ; pathology

Objective To set up reliable cell culture methods of cochlear microvascular endothelial cells in guinea pigs.Methods The cochlear striae vascularies were acquired from guinea pigs with micrergy, the cells were cultured and pured by tissue nubbles cultivation and collagenase digestion.Results Two days after tissue nubbles cultivation of striae vascularis, cells grew at the edge of some tissue nubbles,and proliferated to massive colonies composed of more cells;Some islands made up of a few cells on the bottom of Petri dish were found one to three days after striae vascularies tissue was digested by collagenase;These cells were mainly polygon after purification, monolayer confluent culture cells showed typical "flagstone" appearance as cul tured endothelial cells. According to immunohistochemical detection of factor VIII, which is the mark antigen of endothelial cell, we had observed that the positive reaction cells with cytoplasmic brown pigment were over 95%.Conclusion Cochlear microvascular endothelial cells from guinea pigs can be acquired by tissue nubbles cultivation and collagenase digestion .

Objective To study the relationship between the expression of MDR 1 gene in cochlea lateral wall and the accumulation of adriamycin in endolymph of guinea pig.Methods Twenty four white guinea pigs with red eyes were randomly chosen,and administered an intravenous injection of 30 mg/kg adriamycin or N.S.The animals were sacrificed 24 hours later,the cochlea lateral wall was then immediately removed after the endolymph was drawn by the siphon from the basal turn of cochlea. The expression of the MDR 1 mRNA in cochlea lateral wall of guinea pigs were observed by RT-PCR.The ADM concentration in endolymph was measured by high performance liquid chromatography.Results The accumulation of adriamycin in endolymph negatively correlated with the expression of MDR 1 gene in cochlea lateral wall(r s=-0.901, P

Objective To examine the expression and the significance of mdr 1 mRNA in guinea pig cochlea lateral wall at different developmental stages.Methods Thirty white guinea pigs with red eye including three developmental stages were used in this study. The expression of mdr 1mRNA in the cochlear lateral wall was measured in situ hybridization and analyzed by the softeware of MPLAS-500.Results mdr 1mRNA was detected mainly in the endothelial cells.The expression intensity of mdr 1mRNA was different in guinea pig cochlea lateral wall at different developmental stages, mdr 1mRNA in 1～2 weeks group was significantly lower than that of 3-week-group and 4-week-group(P0.05).Conclusion mdr 1mRNA is found in the normal cochlea lateral wall cells. The expression intensity is different with the development of guinea pig cochlea lateral wall.

Objective To create a method to assay adriamycin in endolymph of guinea pig cochlea,and provide methodology background to study the accumulation of ototoxicity substance in inner ear.Methods 12 normal guinea pig were killed quickly after 24 hours following adriamycin injection.The concentrate of adriamycin in endolymph of guinea pig cochlea was assayed by high performance liquid chromatography(HPLC).Results The linear relation of concentration of adriamycin in endolymph was well when the concentration was from 2.56 ng/mL to 1398.00 ng/mL.Correlation coefficient of linearity was 0.999 (n=10).The percentage of retrieve for adriamycin in endolymph was 96.3%.Relative standard deviation (RSD) was 2.61%.The lowest limitation was 1.9 ng/ml .Conclusion The sensibility of HPLC is high,and it can accurately detect the concentration of adriamycin in endolymph of guinea pig cochlea,especially the adriamycin is administrated in vein.

ObjectiveTo investigate the mechanism of TNF-? on regulating the blood-labyrinth barrier permeability of cochlea of guinea pig.MethodsThe stria vascularis was acquired from guinea pigs and the endothelial cells were cultured of with purified to establish the models of the blood-labyrinth barrier according to our previous researches.The models were treated with TNF-?(0.05,0.1,0.2 ng/ml)for 30,60,90 min or with serum-free DMEM as control.Then their permeability was measured by Evens blue technique.ResultsTNF-? increases Evans blue transfer across endothelial cell monolayer(P

Objective To study the changes of eotaxin expression during different phrases of recovery process of mucosa in sinus cavity after endoscopic sinus surgery. Methods Expression of eotaxin during different phrases of recovery process of mucosa in sinus cavity in 10 patients with type Ⅰ and type Ⅱ chronic sinusitis after endoscopic sinus surgery was determined by immunohistochemitry and Western blotting. Results At 1-2 weeks after surgery, there was a significant difference in expression of eotaxin (P

Objective To investigate the change of the permeability of endotoxin-induced blood-labyrinth barrier(BLB) in guinea pigs.Methods Endotoxin was injected into each tympanic cavity of 27 guinea pigs via superior bulla and Evans blue(EB) was injected via jugular vein before sacrification.Another 27 guinea pigs were served as control by injecting normal saline instead of endotoxin.On 12,24,72 h after injection of endotoxin or normal saline,EB content in BLB was detected after the cochlea were immersed in formamide for 24 h and the pathological change of lateral wall was observed by light microscope and transmission electron microscope.Results The BLB permeability to EB increased in endotoxin-induced cochlea of guinea pigs with prolonged time,and the content of EB was significantly different at 24 h in endotoxin-treated cochlea as compared with that at 12 h and 72 h.No marked morphological changes in the stria vascularis and spiral ligament were observed in control group.The pathologic changes in the lateral wall of cochlea in endotoxin-treated group were inflammatory cell infiltration,blood capillary engorgement,intercellular conjunction cleft.Conclusion The BLB permeability can be increased by endotoxin in the cochlea of guinea pigs,which is mainly caused by the capillary interendothelial cleft and continuous break of endothelial cells in stria vascularis.

This article summarizes proposals of different international guidelines,clinical cases in clinical institutions at home and abroad and the latest research progress of tumor patients with implanted cardiac pacemaker.In addition,the various factors during the implementation of radiotherapy were analyzed.It is possible that reducing the risk grade and minimizing the unfavorable factors for pacemaker could be achieved by designing the scientific treatment plan and setting the reasonable radiotherapy plan.The radiotherapy plan can be established safely and effectively in malignant tumor patients with implanted pacemaker.