1.Management of traumatic optic neuropathy with optic canal decompression through transnasal endoscope: a report of ten cases
Journal of Third Military Medical University 2003;0(18):-
Objective To study the effects of traumatic optic neuropathy treated with optic canal decompression through transnasal endoscope.Methods The data of 10 cases of traumatic optic neuropathy treated with optic canal decompression by transnasal endoscope from April 2003 to February 2004 were retrospectively reviewed.Results In 10 cases,the visual acuity of six cases recovered,and four cases were not improved.Conclusion Optic canal decompression by transnasal endoscope and corticosteroids are beneficial in managing the optic neuropathy.
2.Effect of TNF-? on F-actin of `from guinea pig cochlea
Journal of Third Military Medical University 1984;0(01):-
Objective To observe the changes of F-actin of strial capillary endothelial cells from guinea pig cochlea after TNF-? treatment so as to further study the mechanism of permeability of those cells.Methods Strial capillary endothelial cells were dissociated from guinea pig cochlea and cultured respectively with 0.05,0.1,0.2 ng/ml TNF-? for 90 min,then detected by immunofluorescence laser confocal microscopy for F-actin concentrations.The blank control was set simultaneously.Results TNF-? decreased the content of F-actin in strial capillary endothelial cells(P
3.Effect of TNF-? on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig
Journal of Third Military Medical University 1984;0(02):-
ObjectiveTo investigate the mechanism of TNF-? on regulating the blood-labyrinth barrier permeability of cochlea of guinea pig.MethodsThe stria vascularis was acquired from guinea pigs and the endothelial cells were cultured of with purified to establish the models of the blood-labyrinth barrier according to our previous researches.The models were treated with TNF-?(0.05,0.1,0.2 ng/ml)for 30,60,90 min or with serum-free DMEM as control.Then their permeability was measured by Evens blue technique.ResultsTNF-? increases Evans blue transfer across endothelial cell monolayer(P
4.Changes of eotaxin expression during different phrases of recovery process of mucosa in sinus cavity after endoscopic sinus surgery
Journal of Third Military Medical University 2003;0(20):-
Objective To study the changes of eotaxin expression during different phrases of recovery process of mucosa in sinus cavity after endoscopic sinus surgery. Methods Expression of eotaxin during different phrases of recovery process of mucosa in sinus cavity in 10 patients with type Ⅰ and type Ⅱ chronic sinusitis after endoscopic sinus surgery was determined by immunohistochemitry and Western blotting. Results At 1-2 weeks after surgery, there was a significant difference in expression of eotaxin (P
5.Characteristics of hearing loss caused by local microcirculatory disorders in cochlea of guinea pigs
Journal of Third Military Medical University 2001;23(4):437-439
Objective To study the characteristics of hearing loss caused by local microcirculatory disorders in the cochlea of guinea pigs. Methods The local microcirculatory disorders in the cochlea basal turn near terminal or in the cupule of cochlea of guinea pigs were induced by photochemical reaction. The morphological changes in the cochlea were observed with light microscopy. The compound action potential N1 (CAPN1) amplitude, latency, threshold shift evoked by short tone burst were recorded by Madsen 2250 system. Results When the local microcirculatory disorders took place in the cochlea basal turn near terminal, the hearing losses were more remarkable in the high-range frequencies, but with low-range ones to some extent. If the local microcirculatory disorders was induced in the cochlea cupule, the hearing losses were mainly in the low-range frequencies companying with some high-frequency ones. Conclusion Local microcirculatory disorders in the different parts of the cochlea cause different types of hearing loss in different frequency.
6.Role of cochlea microvasculature permeability changes in guinea pigs after inner ear ischemia
Journal of Third Military Medical University 2001;23(3):339-340
Objective To study the cochlea microvasculature permeability changes of the inner ear in guinea pigs, so as to clarify the possible role of the cochlea microvasculature permeability changes in the ischemic injury of inner ears. Methods Modified method of Evans blue fluorescence was used to observe the changes of permeability of the cochlea microvasculature in the animal model of cochlea microcirculatory disorders which was caused by photochemical reaction. CAPN1 threshold was recorded by using Madsen 2 250 to study the hearing loss. Results The amounts of Evens blue crossing the cochlea microvasculature were (1.709±0.769) and (2.849 ±0.653) μg/per animal 2 and 4 h after the development of cochlea microcirculatory disorder in guinea pigs, respectively (P<0.01); and their hearing loss were (24.44 ±7.27) and (38.33±7.91)dBpeSPL in 2 and 4 h, respectively (P<0.01). Conclusion The permeability of the cochlea microvasculature increases along with the duration of cochlea microcirculatory disorder occur and the increase of cochlea microvasculature permeability might be one of the important mechanisms inducing cochlear ischemic lesions.
7.Isolation, culture and characterization of cochlea microvascular endothelial cells from guinea pigs
Journal of Third Military Medical University 2001;23(3):324-236
Objective To set up a reliable method for isolating and culturing cochlea microvascular endothelial cells. Methods Cochlea stria was separated by micrergy from guinea pigs and the stria tissue nubbles were cultured in vitro. Results Two days later, a few cells were seen disseminately around partial tissue nubbles and their number was increased with time prolongation. There were some cellular colonies consisted of large quantity of cells around the partial tissue nubbles on 10 th day. Under the inversion microscope, the single cultured cells presented a long fusiform and the confluent monolayer cells linked tightly and exhibited the typical structure of “scree” of cultured endothelial cells in vitro. After purification, over 95% of the cultured cells showed factorⅧ relative antigen positive reaction. Conclusion The method used in this study can obtain cochlea microvascalar endothelial cells of guinea pig in vitro.
8.Research progress on radiotherapy for patients with implanted cardiac pacemaker
Chinese Journal of Radiological Medicine and Protection 2017;37(3):237-240
This article summarizes proposals of different international guidelines,clinical cases in clinical institutions at home and abroad and the latest research progress of tumor patients with implanted cardiac pacemaker.In addition,the various factors during the implementation of radiotherapy were analyzed.It is possible that reducing the risk grade and minimizing the unfavorable factors for pacemaker could be achieved by designing the scientific treatment plan and setting the reasonable radiotherapy plan.The radiotherapy plan can be established safely and effectively in malignant tumor patients with implanted pacemaker.
9.Characteristics of Hearing Loss Caused by Local Microcirculatory Disorders in the Cochlea Apical Part of Guinea Pigs
Journal of Audiology and Speech Pathology 1998;0(03):-
Objective To study the characteristics of hearing loss caused by local microcirculatory disorders in the cochlea apical part of guinea pigs. Methods The local microcirculatory disorders in the cochlea apical part of guinea pigs were caused by photochemical reaction. Morphological changes were observed by light microscope. The CAP - N1 amplitude, CAP - N1 latency, CAP - N1 threshold shift and CM amplitude which were evoked by short tone burst were recorded by Madsen 2250. Re- sults Histopathological changes showed that microthrombosis in the capillaries of stria vascularies and spiral ligment were located in the apical and third turn of cochlea. The CAP - N1 amplitude, CAP - N1 latency, CAP-N1 threshold shift and CM amplitude showed that the hearing loss was remarkable in the low - range frequencies. Conclusion When the local microcirculatory disor- ders in the cochlear apical part took place, the hearing loss was more marked in the low - range frequencies.
10.Isolation and Cultivation of Cochlear Microvascular Endothelial Cells from Guinea Pigs by Tissue Nubbles Cultivation and Collagenase Digestion.
Journal of Audiology and Speech Pathology 1998;0(03):-
Objective To set up reliable cell culture methods of cochlear microvascular endothelial cells in guinea pigs.Methods The cochlear striae vascularies were acquired from guinea pigs with micrergy, the cells were cultured and pured by tissue nubbles cultivation and collagenase digestion.Results Two days after tissue nubbles cultivation of striae vascularis, cells grew at the edge of some tissue nubbles,and proliferated to massive colonies composed of more cells;Some islands made up of a few cells on the bottom of Petri dish were found one to three days after striae vascularies tissue was digested by collagenase;These cells were mainly polygon after purification, monolayer confluent culture cells showed typical "flagstone" appearance as cul tured endothelial cells. According to immunohistochemical detection of factor VIII, which is the mark antigen of endothelial cell, we had observed that the positive reaction cells with cytoplasmic brown pigment were over 95%.Conclusion Cochlear microvascular endothelial cells from guinea pigs can be acquired by tissue nubbles cultivation and collagenase digestion .