[Objective]This paper is to detail the system thinking of syndrome differentiation and treatment of eczema from the spleen and provide ideas for clinical diagnosis and treatment of eczema.[Methods]Combined with the understanding of eczema in Chinese and western medicine, detailing the ideas of syndrome differentiation and treatment of eczema from the spleen,which includes the etiology and pathogenesis, the basic rule and its application ideas, to reconcile the five internal organs, the syndrome differentiation of physique and caring spleen and stomach.[Results]The basic pathogenesis of eczema is the weak spleen loss the transport function.In clinical treatment,the main method to complement the spleen is preserving its transport function.On the basis of complementing the spleen,different times have different rules.It should clarify the rule of complementing the spleen to get rid of the wet evil.Types of hot evil or hot and wet evil,the rule is to get rid of the hot and wet evil.After symptom relief,herbs to complement the spleen should be added.Types of wet evil or blood deficiency resulting in wind evil,the rule is to complement the spleen to get rid of the wet evil.Attention to regulating the function of organs, not specifically treating the spleen,but make the spleen as the center to reconcile the five internal organs. The weak spleen is the physical tendencies of eczema patients. Individual differences have targeted treatment to correct the physique bias.Medication and daily life always pay attention to caring spleen and stomach.Diagnosis and treatment of eczema from the spleen can have guiding signficance. [Conclusion]The thinking of syndrome differentiation and treatment of eczema from the spleen has a guide to clinical significance,is worthy of further study and popularization.

The purifying operating room with tidiness, antisepsis and easy operation is widely used in more hospitals. The same time, depurative flow, manufacturing skill and technical standards of it are still developing. But filter maintenance and replacing as key component has major difference in different region and season. Through four-year exploration and practice, the methods of filter maintenance and replacing were explained, which carry out the questions of consumption and energy efficiency.

Objective To explore the effects of oxidative stress and its secondary protein impairment induced by high glucose content in human kidney proximal tubular epithelial cell line (HKC). Methods Cultured HKC were used to detect the reactive oxygen species (ROS) and protein carbonyl content. HKC were divided into three different groups: control group (normal glucose), short-term treatment with high glucose group and long-term treatment with high glucose group. The glucose concentrations in control group and the high glucose treatment groups were 5.5mmol/L and 30mmol/L, respectively. Six time-points were set in short-term treatment with high glucose group namely 30min, 1h, 6h, 12h, 24h and 48h; glucose treatment was instituted for 2 months in long-term treatment of high glucose group. 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) was used as ROS trapping agent, and flow cytometry and laser confocal microscopy were used to detect the concentration of ROS in HKC after treatment with high glucose for different periods of time. Flourescent probe JC-1 was used to test the change in mitochondrion membrane potential (MMP). The protein carbonyl content was determined by 2,4-dinitrobenzene hydralazine (DNPH) chromatometry. Results The ROS level in HKC reached the peak at 1 hour after treatment with high glucose, and it then decreased gradually (P0.05), but after 2 months, there was significant change in the content of DNPH (P

Occult breast cancer is a rare disease with the primary symptom of enlarged ipsilateral axillary lymph node. At present,it is still difficult to diagnose occult breast cancer. The diagnosis is based on the core needle biopsy and following immunohistochemical staining for the enlarged lymph nodes. If the results support,the diagnosis can be confirmed after the exclusion of other primary tumors. The standard treatment of occult breast cancer is still the mastectomy and axillary lymph node dissection. Neoadjuvant chemotherapy is an independent factor affecting the prognosis,which is also recommended before the oper-ation. Chemotherapy,radiotherapy and endocrine therapy could be chosen based on immunohistochemical results after surgical treatment. The prognosis of occult breast cancer is similar to the other types of breast cancer at the same staging.

The principle of temperature control in operating room air decontamination system and the main reasons with difficulties of regulating temperature in transformation period of spring and autumn are analyzed. The reconstructed approaches of circulating water line on surface cooler are adopted appropriately, not only to solve the question of long temperature decreasing response time in operating room but also to save substantially a lot of expenditures and reduce the running loss.

Objective:To study the course and mechanism of the immune response to SARS virus. Methods:The recombinant SARS virus S1 subunit was expressed in E. Coli according to the results of bioinformatics analysis. After purification, the recombinant S1 protein was identified by 6 serum samples of recovered SARS patients and 6 serum samples of health donors, which were collected before out-break of SARS. Results:Sequencing analysis confirmed that the recombinant protein has the same sequence of natural SARS virus S1 subunit. The recombinant S1 protein could react with all the samples from recovered SARS patients but not the control samples from healthy donors according to the results of Western blot. Conclusion:The recombinant SARS virus S1 subunit may provide a good tool for the research of immune response to SARS virus and the producing of recombinant vaccine to prevent people from SARS.

The significance and method of green chemistry teaching are expounded in detail during the course of medical chemistry experiment,and a new experiment system of green chemistry suiting with medicine is designed in the paper.

A series of reforms on the teaching of medical chemistry experiment has been carried out to arouse students' interest in medical chemistry experiment study and improve the quality of instruction,which includes the content of course,the teaching method and the inspection method.Good results have been achieved in the aspect of development of students' comprehensive quality,which lays a good foundation for the study of other courses.

In order to improve teaching quality of medical organic chemistry,problem-based learning was introduced.The characteristic,implementary process and outlines of problem-based learning were discussed in combination with the characteristic of organic chemistry in medical university.

In this paper,the significance of ideological and political education was stated based on the ideological and political status of contemporary college students and the methods of teaching how to actualize the ideological and political education in chemistry teaching were in depth explored.