1.Experience about Filter Maintenance and Replacing in Purifying Operating Room
Chunguang YANG ; Xueyuan LI ; Peng WANG
Chinese Medical Equipment Journal 2003;0(12):-
The purifying operating room with tidiness, antisepsis and easy operation is widely used in more hospitals. The same time, depurative flow, manufacturing skill and technical standards of it are still developing. But filter maintenance and replacing as key component has major difference in different region and season. Through four-year exploration and practice, the methods of filter maintenance and replacing were explained, which carry out the questions of consumption and energy efficiency.
2.Achievement of science and technology innovation mode study-Evaluation of the effectiveness of taking medicine as the example
Xueyuan YI ; Weihua LIU ; Yun PENG ; Peng RUAN
Chinese Journal of Medical Science Research Management 2016;29(2):113-119,124
The current evaluation for scientific and technological achievements and innovation is mainly based on novelty retrieval report conducted by professional organizations.However, such retrieval could not measure the actual value of a scientific and technological achievements comprehensively, such as the practicality and economic value of the outcome;and possibility to be adopted by industry, etc.Current study, from the application aspective, was trying to evaluate the scientific and technological achievements using effectiveness appraisal theory, which emphasis on pre-ethical assessment, demand and adoption assessment, and economic value assessment.It reveals the special phenomenon that that the outcome of innovation may decay over time, however, the effectiveness of innovation would enhance over time.This study also designed a mathematical model and the empirical use.
3.Design of ECG signal acquisition system based on MSP430 microcontroller
Xueyuan JI ; Weidong WANG ; Zhengbo ZHANG ; Guojing WANG ; Fulai PENG
Chinese Medical Equipment Journal 2015;36(5):6-8,46
Objective To design a high performance and low power consumption ECG signal acquisition system which can meet the demand for long time monitoring of the physiological status of patients.Methods The prototype system utilized low power ECG analog front end ADAS1000 and MSP430F5529 microcontroller to achieve configuration of AFE and back-reading of ECG data by SPI bus. Results This system implemented 24-hour dynamic ECG monitoring of patients in active state, and the data acquired were accurate and reliable.Conclusion The system realizes PCB integration, low power consumption, and can be used for battery powered portable application such as wearable devices.
4.Application and development of in vitro metabolism study at early drug discovery stage.
Hui WU ; Ying PENG ; Jianguo SUN ; Xueyuan ZHANG ; Yunxi ZHONG ; Guangji WANG
Acta Pharmaceutica Sinica 2013;48(7):1071-9
Drug metabolism studies, including in vivo and in vitro metabolism studies, are significant in the design of candidate compounds and screening of lead compounds at drug discovery/development stages. Compared with in vivo metabolism studies, in vitro metabolism studies have the advantages of rapidity, simplicity, without consumption of large amounts of samples and animals. Moreover, it is convenient for researchers to observe the selective interaction between compound and target. Therefore, in vitro metabolism studies are appropriate for high throughput screening of compounds which are lack of metabolism information and have been widely used during drug discovery stages. This article briefly introduced the application of in vitro drug metabolism studies based on the metabolic stability, reaction phenotyping and metabolic drug-drug interactions, aiming to raise valuable evaluation strategies for innovative drug discovery in China.
5.Temozolomide Drives Ferroptosis via a DMT1-Dependent Pathway in Glioblastoma Cells
Qingxin SONG ; Shanxin PENG ; Zhiqing SUN ; Xueyuan HENG ; Xiaosong ZHU
Yonsei Medical Journal 2021;62(9):843-849
Purpose:
Temozolomide is used in first-line treatment for glioblastoma. However, chemoresistance to temozolomide is common in glioma patients. In addition, mechanisms for the anti-tumor effects of temozolomide are largely unknown. Ferroptosis is a form of programmed cell death triggered by disturbed redox homeostasis, overloaded iron, and increased lipid peroxidation. The present study was performed to elucidate the involvement of ferroptosis in the anti-tumor mechanisms of temozolomide.
Materials and Methods:
We utilized the CCK8 assay to evaluate cytotoxicity. Levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), iron, and glutathione (GSH) were measured. Flow cytometry and fluorescence microscope were used to detect the production of reactive oxygen species (ROS). Western blotting, RT-PCR and siRNA transfection were used to investigate molecular mechanisms.
Results:
Temozolomide increased the levels of LDH, MDA, and iron and reduced GSH levels in TG905 cells. Furthermore, we found that ROS levels and DMT1 expression were elevated in TG905 cells treated with temozolomide and were accompanied by a decrease in the expression of glutathione peroxidase 4, indicating an iron-dependent cell death, ferroptosis. Our results also showed that temozolomide-induced ferroptosis is associated with regulation of the Nrf2/HO-1 pathway. Conversely, DMT1 knockdown by siRNA evidently blocked temozolomide-induced ferroptosis in TG905 cells.
Conclusion
Taken together, our findings indicate that temozolomide may suppress cell growth partly by inducing ferroptosis by targeting DMT1 expression in glioblastoma cells.
6.Temozolomide Drives Ferroptosis via a DMT1-Dependent Pathway in Glioblastoma Cells
Qingxin SONG ; Shanxin PENG ; Zhiqing SUN ; Xueyuan HENG ; Xiaosong ZHU
Yonsei Medical Journal 2021;62(9):843-849
Purpose:
Temozolomide is used in first-line treatment for glioblastoma. However, chemoresistance to temozolomide is common in glioma patients. In addition, mechanisms for the anti-tumor effects of temozolomide are largely unknown. Ferroptosis is a form of programmed cell death triggered by disturbed redox homeostasis, overloaded iron, and increased lipid peroxidation. The present study was performed to elucidate the involvement of ferroptosis in the anti-tumor mechanisms of temozolomide.
Materials and Methods:
We utilized the CCK8 assay to evaluate cytotoxicity. Levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), iron, and glutathione (GSH) were measured. Flow cytometry and fluorescence microscope were used to detect the production of reactive oxygen species (ROS). Western blotting, RT-PCR and siRNA transfection were used to investigate molecular mechanisms.
Results:
Temozolomide increased the levels of LDH, MDA, and iron and reduced GSH levels in TG905 cells. Furthermore, we found that ROS levels and DMT1 expression were elevated in TG905 cells treated with temozolomide and were accompanied by a decrease in the expression of glutathione peroxidase 4, indicating an iron-dependent cell death, ferroptosis. Our results also showed that temozolomide-induced ferroptosis is associated with regulation of the Nrf2/HO-1 pathway. Conversely, DMT1 knockdown by siRNA evidently blocked temozolomide-induced ferroptosis in TG905 cells.
Conclusion
Taken together, our findings indicate that temozolomide may suppress cell growth partly by inducing ferroptosis by targeting DMT1 expression in glioblastoma cells.
7.Research progress on anti-tumor mechanism of Tubeimoside-1
Liu SUN ; Xueyuan MIAO ; Qing PENG ; Lina LIU ; Qinghuai ZHANG ; Chunze ZHANG
International Journal of Traditional Chinese Medicine 2022;44(1):110-113
Tubeimoside-1 (TBMS1) is a triterpene saponins active components with large content in Cucurbitaceae plant Fritillaria, which is water-soluble and stable. It has a broad inhibitory effect on lung cancer, colorectal cancer, breast cancer, gastric cancer and other tumors. The mechanism is mainly related to the inhibition of tumor cell growth, induction of tumor cell apoptosis, inhibition of tumor cell invasion and metastasis, induction of cell autophagy, and inhibition of tumor angiogenesis.
8.Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment.
Lin HOU ; Xueyuan PENG ; Ruiting WANG ; Yifei WANG ; Hong LI ; Huijuan ZHANG ; Yun ZHANG ; Zhenzhong ZHANG
Acta Pharmaceutica Sinica B 2023;13(7):3137-3152
Type 2 diabetes mellitus (T2DM) therapy is facing the challenges of long-term medication and gradual destruction of pancreatic islet β-cells. Therefore, it is timely to develop oral prolonged action formulations to improve compliance, while restoring β-cells survival and function. Herein, we designed a simple nanoparticle with enhanced oral absorption and pancreas accumulation property, which combined apical sodium-dependent bile acid transporter-mediated intestinal uptake and lymphatic transportation. In this system, taurocholic acid (TCA) modified poly(lactic-co-glycolic acid) (PLGA) was employed to achieve pancreas location, hydroxychloroquine (HCQ) was loaded to execute therapeutic efficacy, and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) was introduced as stabilizer together with synergist (PLGA-TCA/DLPC/HCQ). In vitro and in vivo results have proven that PLGA-TCA/DLPC/HCQ reversed the pancreatic islets damage and dysfunction, thus impeding hyperglycemia progression and restoring systemic glucose homeostasis via only once administration every day. In terms of mechanism PLGA-TCA/DLPC/HCQ ameliorated oxidative stress, remodeled the inflammatory pancreas microenvironment, and activated PI3K/AKT signaling pathway without obvious toxicity. This strategy not only provides an oral delivery platform for increasing absorption and pancreas targetability but also opens a new avenue for thorough T2DM treatment.
9. Silencing lncRNA GIHCG increases radiosensitivity of glioma cells by up-regulating miR-146a-3p
Xueyuan LI ; Qiankun LIU ; Shanpeng YUAN ; Yingwei ZHEN ; Lixin WU ; Wenzheng LUO ; Kang WANG ; Zhuang WANG ; Peng GAO ; Tiansong LIANG ; Dongming YAN
Chinese Journal of Radiation Oncology 2020;29(1):52-56
Objective:
To investigate the effect of lncRNA GIHCG on the radiosensitivity of glioma cells and its mechanism.
Methods:
The expression levels of GIHCG and miR-146a-3p in human brain normal glial cells HEB and glioma cell lines U251, A172, SHG139 and U87 were quantitatively measured by qRT-PCR assay. U251 and SHG139 cells were used for subsequent experiment. After silencing the expression of GIHCG or overexpressing miR-146a-3p in U251 and SHG139 cells, cell proliferation was detected by MTT assay, cell apoptosis was detected by flow cytometry, cell radiosensitivity was detected by colony formation assay and the expression levels of CDK1, CyclinD1, Bcl-2 and Bax proteins were measured by Western blot. The bioinformatics software predicted the presence of a binding site for GIHCG and miR-146a-3p. Dual luciferase reporter gene assay and qRT-PCR assay were adopted to verify the targeting relationship between GIHCG and miR-146a-3p.
Results:
Compared with HEB cells, the expression of GIHCG was significantly up-regulated in glioma U87, U251, A172 and SHG139 cells (all