[Abstract ] Objective As an important metal element, iron is involved in a variety of life activities.This study was to inves-tigate the effect of iron deposition on the apoptosis of PC12 cells and the expression of glyceraldehyde de-3-phosphate dehydrogenase (GAPDH). Methods PC12 cells were incubated with FeCl3 at 50, 100, and 500 μmol/L for the establishment of an iron deposi-tion cell model.The proliferation of the PC12 cells was measured with the CCK-8 method, their apoptosis determined by TUNEL, the expressions of GAPDH mRNA and protein detected by quantitative real-time PCR and Western blot respectively, and the level of GAP-DH in the nuclei measured by immunofluorescence assay. Results Compared with the normal control group, the proliferation rate of the PC12 cells was decreased in the 50μmol/L FeCl3 group (100%vs [77.13 ±3.9]%, P>0.05) and significantly in the 100 and 500 μmol/L groups ([66.19 ±3.37]% and [13.84 ±0.43]%) (P<0.05).In comparison with the control, the expression of GAPDH mRNA was up-regulated in the 50 μmol group (1.00 ±0.00 vs 1.42 ±0.03, P>0.05) and remarkably in the 100 and 500μmol/L groups (1.93 ±0.07 and 2.33 ±0.15) (P<0.05), while that of GAPDH protein down-regulated in the 50μmol group (1.00 ± 0.00 vs 0.94 ±0.11, P>0.05) and markedly elevated in the 100 and 500 μmol/L groups ( 1.45 ±0.13 and 1.79 ±0.07 ) ( P<0.05) .After iron deposition, GAPDH transferred from the cytoplasm to the nuclei of the PC12 cells and nuclear accumulation was observed. Conclusion Iron deposition increases the apoptosis of PC12 cells, which may be related to the up-regulated expression of GAPDH in the nuclei.

The most Cytokines are found to be produced and functional in both of immune systemsand nervous system, which become a foundation of interaction between nervous and immune system.Macrophage and microglia may play an important role in the study of CNS injury and regeneration.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Ankle ; Cognitive Therapy ; Female ; Humans ; Intestine, Large ; physiopathology ; Middle Aged ; Rectal Diseases ; physiopathology ; psychology ; therapy ; Treatment Outcome ; Wrist

Objective To explore the relationship between the dynamic change of plasma tissue-type plasminogen activator(t-PA)and its inhibitor-1(PAI-1)levels and infarct size in patients with acute cerebral infarction.Methods There were 100 patients with acute cerebral infarction,22 cases of large area infarction,36 cases of small area infarction,42 cases of lacunar infarction.The levels of plasma t-PA or PAI-1 in 24 h,2 d,14 d,21 d after cerebral infarction were detected by chromogenic substrate method,compared with normal control and the level of blood plasma t-PA or PAI-1 in patients with different size of cerebral infarction.Results Compared with normal control,levels of plasma t-PA at 24 h,2 d,14 d after acute cerebral infarction decreased significantly,and plasma PAI-1 level increased significantly(all P0.05).The plasma t-PA level in patients with large size of infarction was lower obviously than that in patients with small area infarction and lacunar infarction,small size of infarction was lower than that in lacunar infarction(all P0.05).Conclusion The plasma t-PA level decreased and PAI level increased in patients with acute cerebral infarction.The the size of infarction area is larger,the plasma t-PA level is lower,but there is no relationship between the plasma PAI-1 level and infarction area.

Objective To investigate the effect and significance of batroxobin on the levels of neuron-specific enolase(NSE) and endothelin(ET) in plasm of patients with transient ischemic attacks(TIAs).Methods 120 cases of carotid territory TIAs(

0.05).The scores of MMSE increased significantly(all P

Objective To determine the dynamic changes of plasma endothelin (ET), calcitonin gene related peptide (CGRP) and atial natriuretic peptide (ANP) in elderly patients with acute cerebral infarction and explore the pathological role of ET, CGRP and ANP in the development of acute cerebral infarction.Methods The concentrations of ET, CGRP and ANP in plasma were determined with radioimmunoassay in 78 elderly patients with acute cerebral infarction and 60 normal controls.Results (1)The levels of ET and ANP in patient group were significantly higher than those in control group ( P3 cm) and small area infarct group (1.5～3.0 cm) were significantly higher (all P

Wistar male rats were divided into two grorps, The experimental group was madethe paraplegia model. BOth groups were received cefazolinum 250 mg through single-dosed intra-venous injection. The concentration was measured by HPLC. The result implied that a).the concentra-tion of cefazolinum in paraplegic group was higher than that in control group either the distributingphase or the eliminating phase(p<0. 001);b). the pharmacokinetic parameters showed that the apparent vlumeof distribution:(v1,v2)was decreased and the elimination. half-life(t1/2β)was more length-y in paraplegia group. So that the drug concentration increased. The conclusion is that the dosege on thepatients with paraplegia should be clinically monitored,especilly following hepatic and renal disfunctionand with the drugs with narrowed safety range.

Object To investigate the effects of Heart-benefitting Recipe (HBR) on the histopathological changes of Alzheimer's disease (AD) rats after unilateral amyloid-?_ 25-35 protein (A?_ 25-35 ) injection into the amygdala. Methods The experimental rat models with dementia,spatial learning,and memory impairment were induced by unilateral amyloid ?_ 25-35 protein (A?_ 25-35 ) injection into the amygdala of rats. The spatial learning and memory ability and the function of cholinergic system were observed by the means of Morris water maze and radioligand binding assay. The protein expression of A?_ 1-40 ,AT_8 was estimated with immunohistochemistry. The expression of mRNA related to APP was observed by RT-PCR. Results HBR significantly alleviated the spatial learning and memory impairment induced by A?_ 25-35 and remarkably increased the activity of ChAT and Rt of M-receptor binding sites of these models. The expressions of A?_ 1-40 protein and APP mRNA in cortex and hippocampus of AD rats were decreased remarkably by HBR. The results of immunohistochemistry showed that the expression of hyperphosphorilated tau AT_ 8 in cortex and hippocampus of AD rats was inhibited significantly by HBR. Conclusion HBR can effectively improve the spatial learning and memory impairment,decrease the deposition of A?_ 1-40 ,and alleviate the hyperphos-phorylation of tau of AD rats induced by A?_ 25-35 .

The current evaluation for scientific and technological achievements and innovation is mainly based on novelty retrieval report conducted by professional organizations.However, such retrieval could not measure the actual value of a scientific and technological achievements comprehensively, such as the practicality and economic value of the outcome;and possibility to be adopted by industry, etc.Current study, from the application aspective, was trying to evaluate the scientific and technological achievements using effectiveness appraisal theory, which emphasis on pre-ethical assessment, demand and adoption assessment, and economic value assessment.It reveals the special phenomenon that that the outcome of innovation may decay over time, however, the effectiveness of innovation would enhance over time.This study also designed a mathematical model and the empirical use.