Objective To study the surgical treatment of nodular goiter.Methods The surgical treatment effects of 248 cases with nodular goiter were reviewed.Results 123 paitients were cut off the merely nodle,83 patients suffered one side subtotal lobectomy,32 patients suffered doublr subtotal lobectomy, 10 patients suffered one side merely nodle plus oppside subtotal lobectomy, and 12 patients received operations twice,including 1 case (temporarily) of permanent injury of parathyroid, 1 case(temporarily) of injury of recurrent laryngeal never,3 cases of postoperative hemorrhage.All patients were followed up from 1 to 8 years,and no relapse was noted after operation.Conclusion One side thyroid gland nodle or nodles should cut off the merely nodle and it is unneceeary to deal with the blood vessel of the thyroid up and down,one side or double side thyroid gland nodles tightly should operated subtotal lobectomy, to ligate the blood vessel of the thyroid down, not to dispose the recuyyent laryngeal nerve,reserving the left over gland in cyst,and to prevent hypothyroidism and recurrent laryngeal nerve.

Two methods for the determination of platinum group elements were established based on neutron activation analysis (NAA) and inductively coupled plasma mass spectroscopy (ICP-MS) combined with fire assay preconcentration. Their analytical sensitivity,accuracy and applicability were discussed.The detection limits (ng/g)of NAA for Ru,Rh,Pd,Os,Ir and Pt are 0.5,0.5,0.3,0.1,0.01 and 0.2,respectively.whereas those of ICP-MS are 0.1 for Ru,0.05 for Rh,0.1 for Ir and 0.1 for Pt. Thus, both are complimentary for determination of platinum group elements. By the established methods the contents of platinum group elements in five geological reference materials were determined.

Objective Our previous research demonstrated that trkA and p75 receptors of nerve growth factor(β-NGF) are expressed in human pterygium fibroblasts(HPF), and trkA is expressed only in conjunctiva. The purpose of present study was to investigate the effects of β-NGF on proliferation of HPF and analyse the pathogenesis mechanism of pterygium. Methods The HPF specimen was obtained from Union Hospital of Tongji Medical College, Huazhong University of Science and Technology during the surgery. Explant culture technique was used for the primary culture of HPF tissue. The cells of confluenting 80% were collected and digested using 0. 25% tripsin + 0. 02% EDTA (1:1) and the third to fifth generation of cells were utilized in the experiment. Different concentrations of β-NGF was added in medium. Cultured cells were identified using vimentin, keratin and α-SMA. MTT was used to determine the proliferation of HPF after addition of β-NGF. The expression of trkA and p75 in HPF was detected by immumofluorescence method. Cell proliferation also was semi-quantitatively analyzed by detect of expressions of PCNA protein and mRNA in HPF using Western blot and RT-PCR. Results Cultured HPF cells showed the positive responses for vimentin, α-SMA, trkA and p75 but absent reaction for keratin. MTT revealed that the OD value of HPF cells was gradually enhanced with the increase of β-NGF concentration in 12, 24, 48, 72 and 96 hours after β-NGF action with the maximum stimulation at 48 hours. The expression of PCNA protein and mRNA in HPF was significantly different among various concentrations of β-NGF groups(F_(protein) = 24. 980, P = 0. 000; F_(mRNA) = 64. 490, P = 0. 000) and increased from 5 ng/mL β-NGF group through 50 ng/mL β-NGF group in comparison with 0 and 1 ng/mL β-NGF group (P ＜ 0. 05) . Conclusion The findings demonstrate the potential proliferative effect of β-NGF binding to trkA and p75 on HPF.

Objective To investigate the characteristic changes of the auditory brainstem response (ABR) of normal hearing newborn and infants .Methods One hundred and forty -five cases (290 ears) normal hearing new-born and infants were enrolled for ABR test .Before that they passed the distortion product otoacoustic emissions and acoustic immitance test previously .According to age ,13 (26 ears) of 145 were included in the 1~7 days age group ,17 (34 ears) was in the 30~59 days age group ,20 (40 ears) in the 2~4 months age group ,19 (38 ears) in the 5~7 months age group ,17 (34 ears) in the 8~10 months age group ,18 (36 ears) in the 11~13 months age group ,12 (24 ears) in the 14~16 months age group ,13 (26 ears) in the 17~19 months age group ,16 (32 ears) in the more than 20~24 months age group .The control group was 20 (40 ears) normal hearing young persons (18~25 years old) .The variation of the peak latency and interpeak latency of waves were observed ,and compared with the normal young people .Results In 70 dB nHL click sound stimulus ,the peak latency and interpeak latency ofⅠ ,Ⅲ ,Ⅴ waves of normal hearing neonates and infants progressively shortened as they grew older .As they ap-proach 18 months or more old age ,their peak latency and interpeak latency of Ⅰ ,Ⅲ ,Ⅴ waves may be same as nor-mal young people .Conclusion With the growth of the age ,the peak latency and interpeak latency of Ⅰ ,Ⅲ ,Ⅴwaves of normal hearing newborn and infants gradually shortened .The reason may be related to the maturity of the auditory system .

Objective To understand the abnormal status of alanine aminotransferase (ALT),fasting blood glucose (GLU),to-tal cholesterol (TC)and triglyceride (TG)in urban and rural residents,to provide the basis for making the effective prevention measures,health education and health promotion to community work more targeted and effective.Methods The data of physical ex-amination results of 4 042 residents were analyzed retrospectively,the levels and abnormal rates of ALT,TG,TC,GLU were com-pared between different gender and age.Results Among 4 042 residents,the abnormal rate of male was significant higher than that of female,but the abnormal rates of TG,TC of female were significant higher than those of male(P <0.05).The highest rates of ALT,TG,TC,GLU in male were in 20-<40 years old,40-<60 years old,60-<80 years old,≥80 years old.The highest rates of ALT,TG,TC,GLU in female were in 0-<20 years old,60 -<80 years old,≥ 80 years old,≥80years old.Conclusion The health status of urban and rural residents is not optimistic,the community intervention,health guidance should be strengthened to prevent and control the occurrence and development of diseases and improve the health level of urban and rural residents.

Objective To analyze the correlation between blood lead,zinc levels and tic disorders(TD) in children.Methods One hundred and fourteen children with TD were chosen as observation group,and 100 healthy children were chosen as control group.The serum levels of lead and zinc were compared between the two groups,and the correlation between blood lead,zinc levels and TD was analyzed.Results The serum level of blood lead in the observation group was (70.8 ± 14.9) μg/L,which was higher than (50.9 ± 13.8),μg/L in the control group,the difference was statistically significant(t =4.932,P ＜ 0.05).The serum level of blood zinc in the observation group was (461.4 ±33.2)μg/L,which was lower than (501.5 ± 34.1)μg/L in the control group,the difference was statistically significant(t =5.217,P ＜ 0.05).The serum level of blood lead ≥60μg/L and the serum level of blood zinc ＜470μ g/L were significantly related with TD(OR =2.192,2.012,all P ＜ 0.05).Conclusion The blood lead and zinc levels are significantly correlated with TD in children.

AIMTo investigate the existence of TMPRSS2:ERG fusion gene in circulating tumor cells (CTC) from prostate cancer patients and its potential in monitoring tumor metastasis.

METHODSWe analyzed the frequency of TMPRSS2:ERG and TMPRSS2:ETV1 transcripts in 27 prostate cancer biopsies from prostatectomies, and TMPRSS2:ERG transcripts in CTC isolated from 15 patients with advanced androgen independent disease using reverse transcription polymerase chain reaction (RT-PCR). Fluorescence in situ hybridization (FISH) was applied to analyze the genomic truncation of ERG, which is the result of TMPRSS2:ERG fusion in 10 of the 15 CTC samples.

RESULTSTMPRSS2:ERG transcripts were found in 44% of our samples, but we did not detect expression of TMPRSS2:ETV1. Using FISH analysis we detected chromosomal rearrangements affecting the ERG gene in 6 of 10 CTC samples, including 1 case with associated TMPRSS2:ERG fusion at the primary site. However, TMPRSS2:ERG transcripts were not detected in any of the 15 CTC samples, including the 10 cases analyzed by FISH.

CONCLUSIONAlthough further study is required to address the association between TMPRSS2:ERG fusion and prostate cancer metastasis, detection of genomic truncation of the ERG gene by FISH analysis could be useful for monitoring the appearance of CTC and the potential for prostate cancer metastasis.

Base Sequence ; DNA Primers ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Neoplastic Cells, Circulating ; Oncogene Proteins, Fusion ; genetics ; Prostatic Neoplasms ; blood ; genetics ; Reverse Transcriptase Polymerase Chain Reaction

The purpose of this study was to investigate the NOAEL of the nickel ion and provide with basic data for the biological evaluation of those medical devices containing nickel. Five groups SD rats were repeatedly exposed during 14 d respectively to nickel at first stage doses of 4.9, 3.7, 2.5 mg/(kg.d), and the second stage doses of 1.2, 0.25 mg/(kg.d) by the intravenous route. The results showed that the NOAEL of nickel ion is 0.25 mg/(kg.d) for SD rats, and the result was verified by subchronic systemic toxicity test of nickel alloy. The threshold of toxicological concern (TTC) of nickel is 150 μg/d (based on application of 100-fold uncertainty factor and a body weight of 60 kg)deduced by these data.
Animals ; Equipment and Supplies/adverse effects* ; Nickel/toxicity* ; No-Observed-Adverse-Effect Level ; Rats ; Rats, Sprague-Dawley ; Risk Assessment