From the chloroform-soluble fraction of the ethanol extracts of the whole plant of Taxus chinensis var. mairei (Lemee et Levl), four compounds were isolated by using repeated column chromatography on silica gel and Sephadex LH-20. Based on spectroscopic data (UV, IR, ESI-MS, 1H NMR and 13C NMR), the compounds were identified as taxamairin K (1), 2α, 4α-dideacetoxy-7β-benzoyloxy-5β,20-epoxy-9α,10β,13α,15-tetrahydroxy-11(15→1)abeotaxa-11-ene (2), 7β-xylosyl-taxol (3), 10-deacetoxy-7-xylosyl-taxol (4). Among them, taxamairin K is a new compound.

Objective To intensively investigate the chemical constituents in the heartwood of Taxus yunnanensis Cheng et L.K.Fu.Methods The heartwood was extracted with ethanol,the ethanol extract was subjected to the extraction with methylene trichloride,which was submitted to chromatography on sili-ca gel and Sephadex LH-20 column to isolate some compounds.And their structures were elucidated on the basis of spectral analysis(UV,IR,ESI-MS,1H-NMR,13C-NMR).Results The compounds were identified as 2?,5?,7?,9?,10?,13?-hexaacetoxy-4(20),11-taxadiene(Ⅰ),taxusin(Ⅱ),taxa-4(20),11-diene-2?,5?,10?-triacetoxy-14?,2-methybutyrate(Ⅲ),10?-hydroxy-2?,5?,14?-triacetoxy-4(20),11-taxadiene(Ⅳ),1-dehydroxybaccatin Ⅳ(Ⅴ),baccatin Ⅳ(Ⅵ),baccatin Ⅵ(Ⅶ),7,9-deacetylbaccatin Ⅵ(Ⅷ),10-deacetyltaxuyannine(Ⅸ),1?-accetoxy-5-deacetyl-baccatin Ⅰ(Ⅹ),baccatin Ⅰ(Ⅺ),taxuchin A(Ⅹ Ⅱ),secoisolariciresinol(Ⅹ Ⅲ),?-conidendrin(Ⅹ Ⅳ),isotaxiresinol(Ⅹ Ⅴ),lariciresinol(Ⅹ Ⅵ),sequoitol(Ⅹ Ⅶ),?-sitosterol(Ⅹ Ⅷ).Among them compounds Ⅰ,Ⅴ,Ⅵ,Ⅺ,Ⅹ Ⅱ,Ⅹ Ⅵ were obtained from the heartwood of T.yunnanensis for the first time.Conclusion The chemical constituents in the heartwood differ from the other parts of T.yunnanensis,but there is little difference within the species of Taxus L.

Objective:To establish a three-dimensional quantitative structure-activity relationship (3D-QSAR) model for thiazide 11β-hydroxy steroid dehydrogenase ( HSD) inhibitors in order to perform structure modification and find thiazide 11β-HSD inhibitors with more activity. Methods: The 3D-QSAR model of thiazine derivatives was constructed by the method of comparative molecular force field analysis, and the model was validated by using a molecular docking method. Results:An accurate 3D-QSAR model of 11β-HSD inhibitors was obtained (CoMFA:q2 =0. 346, r2 =0. 850, where q2 was the cross-validation coefficient and r2 was the non-cross validation coefficient) . Conclusion:The results provide important theoretical basis for the rational design of novel thiazide 11β-HSD inhibitors.

OBJECTIVE To explore the management methods of sterilization and supply department for the control of hospital infection. METHODS Since 2005, strict infection management system in all aspects by the sterilization and supply department has been taken including renewable medical equipment from recycle to distribution, sterile disposable medical equipment from acceptance to distribution, and the district environment and self-protection of its own staff in the sterilization and supply department and so on. RESULTS Hospital infections had not occurred as a result of sterilized supply for three years. CONCLUSIONS Since strict control and management system is carrying out, measures are effective, and good results have been achieved, which provide important guarantees for effectively controlling infection and medical safety.

OBJECTIVE To analyze the cause of failure of bacteria-killing by autoclave.METHODS Under the process of using the fast-reading bio-indicator to take a routine inspect for the autoclave,while the pressure,the temperature and the time for the bacteria-killing were up to the standard,and the result of the inspection was respectively negative and positive.RESULTS For the sudden malfunction of the exhaust valve,the cold air mass was left in the experimental parcel.Therefore,the result was respectively negative and positive.CONCLUSIONS The more strict examination should be taken for the bacteria-killing tank and the advanced B-D process inquiry-device and the bacteria-killing process inquiry device in the world should be adopted to ensure that the process of bacteria-killing is qualified at all times.

OBJECTIVE To effectively control the quantum of bacteria existing in the air of the cleaning section in the disinfecting supply division. METHODS The standards and methods set in the Hospital Disinfecting Sanitation Standard(GB15982-1995) were used. RESULTS Scientific,effective and strict control measures should be implemented to make the quantum of bacteria existing in the air to meet the Environment Standard Type Ⅱ. CONCLUSIONS This article provides the credible data for boosting the pureness of the air of the cleaning section of disinfecting supply division.

OBJECTIVE To explore the impact factors on the process of washing and disinfection for medical instruments.METHODS The reasons that tend to cause bad washing quality were analyzed in the process of washing for medical instruments and corresponding measures were taken.RESULTS Efficient washing procedures should be installed in the process of washing for medical instruments and washing effects should be affirmed.CONCLUSIONS Efficient washing methods should be taken to guarantee successful sterilization and control hospital infection.

ObjectiveTo probe the value of transrectum ultrasound (TRUS) in evaluating the effect of new adjuvant therapy (NAT) in treating middle-low level rectal cancer (MLLRC).MethodsTRUS was used to examine 82 cases of MLLRC before and after NAT.All cases were pathologically confirmed.The results of TRUS before and after NAT were compared.ResultsThe diagnostic rate of TRUS in MLLRC was 97.6％(80/82).The up to down range and thickness of foci of 80 cases which were detected by TRUS before NAT showed different degree diminution after NAT( P ＜0.05).The number of lymph node around rectum decreased obviously ( P ＜0.05).Only the lymph nodes of 5 cases of all lymph nodes detected by TRUS before and after NAT showed dot flow signal.ConclusionsTRUS can clearly showed the MLLRC foci,internal flow signal and lymph nodes around rectum.This is of important value in evaluating the effect of NAT,choosing the therapeutic schedule and avoiding over therapy.

This study was aimed to reveal the effects and molecular regulation mechanism of methyl jasmonate (Me-JA) on volatile terpenoids from Amomum villosum Lour. After the leaves and fruits of A momum villosum Lour. were treated with different concentrations of MeJA, the volatile terpenoids of fresh fruits from A . villosum Lour. were ex-tracted with microwave method and analyzed by GC-MS. Then, leaves and fruits treated with MeJA were sequenced by Illumina. The transcriptome data was analyzed by bioinformatic methods. The results showed that there were 20 and 33 volatile terpenoids detected in peels and seed groups, respectively. Contents of volatile terpenoids in peels and seed groups were both improved after 600 μmol·L-1 MeJA treating fruits for 24 h, such as bornyl acetate, cam-phor, borneol, and etc. While 200 μmol·L-1 MeJA treating different parts for 24 h can regulate the biosynthesis of some volatile terpenoids in peels differently. And 200 μmol·L-1 MeJA treating fruits can improve the content of ma-jor volatile terpenoids in seed groups. A total of 68 168 unigenes were obtained with de novo assembly, and 48 627 unigenes were annotated after comparison with public protein databases. Analysis of functional annotation against KEGG database showed that there were 208 unigenes closely related with metabolism of volatile terpenoids and 22 u-nigenes related with MYC2 transcription factors. It was concluded that MeJA can effectively regulate the metabolism of volatile terpenoids from A . villosum Lour. There were a lot of candidate genes related with the biosynthesis of volatile terpenoids obtained by analyzing the transcriptome data which also provided a large amount of data for the discovery and regulation of functional genes related with the biosynthesis of volatile terpenoids from A . villosum Lour.

Objective To probe the value of color Doppler flow imaging(CDFI) in diagnosis of borderline ovarian epithelial tumors (BOET).Methods Thirty-six cases of BOET,139 cases of ovarial cystadenoma and 66 cases of ovarial cystadenocarcinoma were diagnosed pathologically.All cases were preoperatively detected by CDFI.Tumor sign CA125 was also detected.The size,boundary,internal echo and internal flow distribution were observed.The results of CDFI of different diseases were contrasted.Results Ultrasonic diagnosis rate was 75 ％ (27/36) in BOET,90.6 ％ (126/139) in ovarial cystadenoma and 92.4 ％ (61/66) in ovarial cystadenocarcinoma.Positive rate of CA125 of ovarial cystadenocarcinoma was obviously higher than those of BOET and ovarial cystadenoma.Positive rate of CA125 of ovarial cystadenocarcinoma containing solid was especially higher than that of BOET containing solid.Ovarial cystadenoma mainly showed single cavity,few or without solid as well as hematoasthenia or asanguineous.BOET and ovarial cystadenocarcinoma mainly showed multi-cavity,more solid as well as plentiful blood flow.Blood flow of BOET distributed regularly comparing with ovarial cystadenocarcinoma.Conclusions Characteristics of CDFI combining results of CA125 can provide powerful reference for diagnosise and differentiate diagnosis of BOET and more information diagnostic information for clinic to chosing treatment scheme and postoperative long-term follow-up.