Intensive blood pressure control reduces macrovascular and microvascular complications in diabetic patients.A goal blood pressure of

Objective:To study the different gene expressions in human periodontal cells after being treated with Emdogain(EMD). Methods: The fourth passage periodontal cells were cultured. Cells in experimental group were cultured with 100 μg/ml EMD in non-serum DMEM for 3 days. Cells in control group were cultured in non-serum DMEM for 3 days. The gene expressions in human periodontal cells were screened with gene chip technique. Results: There were 112 differently expressed genes, 11 down-regulated genes and 101 up-regulated genes. Conclusion: After treated with Emdogain, expression of genes related to cell growth, cell proliferation, biosynthesis of extracellular matrix, angiogenesis and osteogenesis is up-regulated dramatically. Genes related to lipid metabolization and keratinocyte growth is down-regulated in human periodontal cells.

Objective: To study the effects of Simvastatin on the proliferation and osteoblastic differentiation of human periodontal ligament (PDL) cells. Methods: The fourth passage human PDLs were cultured in conditioned mineralization medium with different concentrations of Simvastatin. Proliferation ability was detected by MTT method. Alkaline phosphatase (ALP) activity was measured with 4-nitrophenyl phosphate hexahydrate (PNPP) assay. Results: Proliferation and osteoblastic differentiation of human PDLs were increased in all treatment groups with different concentrations of Simvastatin(10-9, 10-8, 10-7, 10-6 mol/L). Compared with control group, significant statistical difference was found in 10-8 mol/L, 10-7 mol/L and 10-6 mol/L groups(P<0.05). The maximum effect was observed at the concentration of 10-7 mol/L. Conclusion: Optimal concentration of Simvastatin can improve the proliferation and osteoblastic differentiation of human PDLs.

Objective To investigate the operative methods and merits of nasal reconstruction in terms of the aesthetics. Methods The noses of 12 patients were reconstructed with an expanded triangle-shaped forehead flap with unilateral supratrochlear artery, a skin expander was placed obliquely under galea aponeurotica of forehead. Liquid was injected with conventional expansion method. Using the skin and the scars on nasal dorsum and tip as lining, based on intercanthal distance and aesthetic standard, a triangle-shaped forehead myocutaneous flap was designed over the expanded forehead skin tissue and used for a nasal reconstruction. The triangle-shaped flap was trimmed to different layer and reshaped based on aesthetic subunit.Results In twelve post-operative patients with nasal defect, no flap necrosis was found and the appearance of reconstructed noses were almost normal and satisfactory after follow-up for 6 months to 2 years. Conclusion The modified forehead myocutaneous flap according to aesthetic standard is safe and ideal for major nasal reconstruction. Meticulous moulding of triangle-shaped flap, nose interior with good blood supply, and primary insertion of nose stretcher are the key to a satisfactory appearance.

BACKGROUND:The physicochemical properties and bioactivity of titanium surface will degrade with time because of the aging phenomenon,affecting the efficiency of implant-bone osseointegration.Therefore,the re-activation of the implant surface physicochencical properties and bioactivity is important.OBJECTIVE:To investigate the re-activation effect of ultraviolet rays on aging titanium surface.METHODS:Sand-blasted and acid-etched titanium discs were divided into three groups randomly:Group A was new titanium surface,Group B was stored in a sealed container for 4 weeks,Group C was treated with ultraviolet rays for 15 minutes after stored in the sealed container for 4 weeks.The surface roughness,elemental composition and surface energy of the titanium surface were examined by optical profilometer,X-ray photoelectron spectroscopy and contact angle measuring device,respectively.The bioactivity of the titanium surface was examined by cell culture experiments.MG63 osteoblast-like cells were cultured on the different titanium surfaces.After 30 minutes,1,2,4 hours of incubation,the cells were strained with Hoechst33342 fluorescence,and initial attachment of cells was evaluated by measuring the amount of cells attached to the titanium surface.The proliferation of cells was quantified in terms of cell density at 1 and 3 culture days using tetrazolium salt (MTS)-based colorimetry.RESULTS AND CONCLUSION:There was no obvious difference in the surface roughness of the three groups,and ultraviolet treatment did not change the surface morphology of titanium surface.X-ray photoelectron spectroscopy showed that the C element content of group A and group C was lower than that of group B (P ＜ 0.05),and the content of Ti,O,N elements was significantly higher than that of group B (P ＜ 0.05).No difference in the surface element composition between group A and group C was found.Both of the surface contact angles of group A and group C were Oo,but in group B,the value was 115°.The number of initially attached cells of group A and group C was significantly higher than that of group B after incubated 30 minutes,1,2 and 4 hours (P ＜ 0.05),and no difference between group A and group C was found.The proliferation of cells of group A and group C was significantly higher than that of group B after incubated 1 and 3 days (P ＜ 0.05),but no difference between group A and group C was found.In conclusion,ultraviolet rays show good effects on the re-activation of the aging titanium surface,which can reduce hydrocarbon contamination and recover the high surface energy to increase cell adhesion and proliferation.

Objective To investigate the therapeutic effect of the ultra-early stent-assisted coil embolization of the ruptured intracranial aneurysms. Methods The clinical data of 13 patients with ruptured intracranial aneurysm treated by ultra-early stent-assisted coil embolization were analyzed retrospectively. The preoperative Hunt-Hess gradeⅠ-Ⅱ was in 7 cases,gradeⅢ was in 4 cases,and grade Ⅳ was in 2 cases. The patients were treated with stent-assisted coil embolization under the general anesthesia with endotracheal intubation within 24 h of aneurysm rupture. The postoperative embolization was assessed according to the Raymond grading standard. The postoperative complications and the assessment of the follow-up results from 1 to 6 months after procedure according to the modified Rankin scale (mRS ) scores were observed. Results All 11 patients recovered well,1 case had postoperative hemiplegia,1 case had postoperative bleeding,and none of them died. During the follow-up period,no patients had rebleeding, 1 had recurrence,and DSA revealed that the patient was embolized completely at 2 months after reembolization. Conclusion The method of ultra-early stent-assisted coil embolization of ruptured intracranial aneurysms is feasible. It may improve the cure rate of the ruptured aneurysms and improve the prognosis of patients.

Objective To assess the diagnostic value in breast nodules with ultrasontic scoring.Methods Benign and malignant breast nodules were studied with ultrasonic scoring and compared with pathologic results.Results The 2D-ultra-sontic scoring and total ultrasontic scores of benign breast nodular lesions had significant difference compared to malignant breast nodular lesions (P<0.01).Ultrasontic scores of malignant breast nodular lesions were higher than those of benign breast nodular lesions (P<0.01).According to the ROC curve and Youden index,2D-ultrasonic scores ≥11 could be considered as the critical point for the diagnosis of malignant breast nodular lesions,the total ultrasonic scores ≥12 could be considered as the critical point for diagnosis of malignant breast nodular lesions.Conclusion Ultrasonic scoring for breast nodular lesions may be useful for improving the diagnosis accuracy of the benign and malignant breast nodular lesions.

Objective To explore the use of rhomboid skin flap in expanded skin flap transfer. Methods A rhomboid skin flap was designed if the top soft part could not be fully utilized after expanded in a rotation skin flap. The flap pedicels were designed near the incision side. It should be ensured that ra-tio of the length to the width of the composite flap, which was composed of the rhomboid skin flap and the rotation skin flap, was 2.5∶1.0. Results Among these 11 patients with re-designed rhomboid skin flaps in the rotation skin flaps, the ratio of the length to the width reached to 3∶1 in some cases, but 2. 5∶1.0 in most cases. All the skin flaps survived, except one patient with disturbance of blood circulation in a small area and one with mild congestion. Conclusion The expanded soft tissue can be fully and rationally utilized to repair the skin defect in this design. Attention should be paid to the ratio of the length to the width of the composited flap, and it is better to select axial flap as the composite flap for safety. This method is safe, and worthy of recommendation.

ObjectiveTo investigate the influence of connective tissue growth factor (CTGF) onthe collagen metabolism in human keloid fibroblasts with RNA interference (RNAi).Methods Human keloid fibroblasts (KFB) in vitro were transfected by 3 pairs of specific small interfering RNA (siRNA) CTGF plasmid synthesized for human CTGF,respectively.Reverse transcriptase-polymerase chain reaction (RT-PCR) contributed to the screening of the best siRNA in interfering of CTGF expression in human keloid fibroblasts to construct the plasmids,with the application of RNAi,to test the changes of expression level and collagen content of CTGF in transfected keloid fibroblasts through RT-PCR and Western blotting compared to its control groups.ResultsThe 3rd pair (C3) siRNA- CTGF expression of genes and proteins was remarkably inhibited after being interfered with human keloid fibroblasts,with inhibitory rates of 86.8 ％ and 65.6 ％.ConclusionsKeloid fibroblasts transfected by plasmid siRNA-CTGF effectively inhibite the expression of CTGF and deposition of collagen,and CTGF promotes the collagen synthesis in keloid development.

Objective: To investigate the relationship between TIMP-1 gene polymorphisms and susceptibility of chronic periodontitis(CP) in Han Cantonese. Methods: Buccal swabs from 70 CP patients and 74 periodontal healthy controls were collected. DNA was extracted from these buccal swabs by using Chelex-100. TIMP-1 +372T/C (rs4898)、TIMP-1+533C/T (rs1062849) polymorphisms were tested by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). Allele distribution and genotypes frequencies in the patients and controls were analyzed. Results: Frequency variance of allele T and C at site of TIMP-1 +372 in patients and controls showed no statistical difference. TIMP-1+533C/T polymorphism of TIMP-1 hadn't been found in the present study. Conclusion: There is no relationship between TIMP-1 +372T/C polymorphism and susceptibility of chronic periodontitis, and TIMP-1 +533C/T polymorphism doesn' t exist among Han Cantonese.