Objective To observe the effects of different H.pylori strains (cagA + and cagA -) infection on Fas/FasL expression and the pathogenesis of gastirc carcinoma. Methods Gastric biopsy specimens were taken from 80 patients, and these patients were divided into 9 groups according to the H E staining and H.pylori infection. H.pylori (+) groups were subdivided into cagA + and cagA - groups. RUT, PCR and histology were used to detect H.pylori and PCR for cagA detection. Immunohistochemical staining was used to detect Fas/FasL expression. Results The infection rate of H.pylori was 60.0%, and rate of cagA(+) is 90.47%. H.pylori (+) specimens express Fas/FasL more higher than H.poylori (-) ones ( P 0.05). Conclusions Our data suggest that H.pylori infection can induce the expression of Fas/FasL in gastric epithelial cells. Gastric adenocarcinoma express high amount of FasL, which may plays an important role in immune escape of stomach cancer.

Objective To observe the response of and recurrence in patients with localized eczema after treatment with topical glucocorticoid alone or in combination with topical antibacterials.Methods Totally,217 outpatients with acute or subacute localized eczema were enrolled and randomly classified into two groups to be treated with topical corticosteroids combined with mupirocin cream (experiment group,n=191) or topical corticosteroids alone (control group,n =190) for two weeks.The patients who were cured at the end of the 2-week treatment were followed for 13 weeks for the observation of recurrence.Chi-square test and Student's t test were conducted to compare the cure and recurrence rates between different groups of patients.Results After two weeks of treatment,the cure rate in the experiment group was significantly higher than that in the control group (80.6％ vs.64.8％,x2 =6.576,P =0.01).Increased cure rate was also observed in patients with acute and subacute eczema receiving glucocorticoidCaspase14antibiotic combination therapy compared with those receiving corticosteroids alone.During the 13Caspase14week follow up,a lower recurrence rate was noted in the experiment group compared with the control group (15.6％ vs.33.8％,x2 =6.455,P ＜ 0.05).Further more,the patients experiencing relapse of eczema showed a higher proportion of hand eczema cases (29.4％ vs.13.0％,x2 =4.982,P ＜ 0.05)compared with those without relapse.Adverse reaction was mild in these patients,and no statistical difference was observed between the experiment group and control group (5.71％ vs.4.46％,x2 =0.176,P ＞ 0.05).Conclusion The combination with topical antibacterials may enhance the therapeutic effect of topical glucocorticoids on and reduce the recurrence of localized eczema.

Objective To investigate the efficacy and safety of long-term intermittent treatment with topical corticosteroids in localized chronic eczema.Methods A total of 129 patients with localized chronic eczema were treated with triamcinolone acetonide and econazole nitrate cream (Pevisone (R)).Those who achieved a clinical cure within 4-week treatment were recruited into the following long-term study and classifled into 2 groups to be intermittently treated with triamcinolone acetonide and econazole nitrate cream or a moisturizing cream for 8 weeks.The cream was applied topically twice a day for 2 days every week.Patients were followed up on week 4 and 8 after the beginning of treatment and on week 12 after the discontinuation of treatment.The severity of eczema in patients was rated according to SCORAD (SCORing atopic dermatitis) score.Results The SCORAD score was significantly lower in patients treated with triamcinolone acetonide and econazole nitrate cream than in those with moisturizing cream on week 8 in the treatment and week 12 after the discontinuation (t=3.076,2.367,both P<0.05).A statistical decrease was also observed in the recurrence rate of eczema in patients treated with triamcinolone acetonide and econazole nitrate cream compared with moisturizing cream.treated patients at the 3 follow-up time points (x2=4.426,7.683,8.199,all P<0.05).The incidence of adverse events was 3.1% during the treatment with triamcinolone acetonide and econazole nitrate cream.No severe adverse reactions were observed.Conclusion Long-term intermittent treatment with topical corticosteroids is effective for the prevention of exacerbation and postponement of recurrence,of eczema.

Objective To quantitatively investigate the expression levels of the cytosine deaminases,hA3B,hA3F and hA3G in peripheral blood mononuclear cell(PBMC)of human immunodeficiency virus(HIV)infected patients and to analyze the correlation between cytosine deaminases expression and CD4~+ T lymphocyte counts. Methods Peripheral blood samples were collected from 21 HIV-infected subjects who didn't take antiretroviral therapy(ART-),21 HIV-infected subjects receiving ART(ART+),and 10 HIV-uninfected subjects. PBMC were isolated by Ficoll density gradient centrifugation, followed by RNA extraction and cDNA synthesis.hA3B,hA3F and hA3G mRNA levels were determined by real-time fluorescent quantitative polymerase chain reaction(PCR).CD4~+ T lymphocyte counts were determined using flow cytometry. Data were analyzed by t test, t' test or Wilcoxon rank sum test. Results In HIV-infected subjects without or with ART,HIV-uninfected subjects, the levels of hA3B mRNA were 208.4,365.2 and 563.6,hA3F mRNA were 245.5,316.6 and 442.9,hA3G mRNA were 404.6,360.8 and 638.6,respectively.hA3G mRNA level in HIV-infected subjects was lower than that in HIV-uninfected controls(P=0.0131),but there was no statistical difference between ART+ and ART-groups(P=0.7342).There were no correlations between hA3B,hA3F and hA3G mRNA levels and CD4+ T lymphocyte counts in either ART-or ART+HIV-infected subjects(ART-:r=-0.0104,r=-0.0545,r=0.1623,all P＞0.05;ART+:r=0.3220,r=0.2193,r=0.1455,all P＞0.05).In addition,hA3B,hA3F and hA3G mRNA levels were positively correlated with one another in ART-HIV-infected subjects and HIV-uninfected controls(P＜0.05),but not in ART-HIV-infected subjects(P＞0.05).Conclusions hA3B,hA3F and hA3G expression levels do not directly correlate with CD4~+ T lymphocyte counts in HIV-1-infected patients,hA3B,hA3F and hA3G expression levels in PBMCs tend to he decreased after HIV-1 infection, and ART may increase hA3B and hA3F mRNA expression.

Objective To estimate effects of Qingpeng ointment on skin barrier function,and to explore their potential mechanisms.Methods A total of 12 female volunteers with xerosis on the extensor surfaces of legs were enrolled into this study.All the volunteers were topically treated with Qingpeng ointment on the right leg (Qingpeng side) and its vehicle on the left leg (vehicle side) twice daily for 7 consecutive days.Skin barrier function-associated indexes including transepidermal water loss (TEWL) and the water content of the stratum corneum were measured before the treatment,after 3-and 7-day treatment separately.Thirty-six BALB/c female mice were randomly and equally divided into 6 groups:a blank control group receiving no induction or treatment,a model group induced by 2,4-dinitrofluorobenzene (DNFB) on the back for 5 consecutive weeks,3 Qingpeng groups and a vehicle group induced by DNFB on the back for 5 consecutive weeks followed by topical treatment with different concentrations (50％,75％,100％) of Qingpeng ointment or its vehicle twice a day for 2 consecutive weeks.At the end of treatment,skin appearance of mice was observed with naked eyes,skinfold thickness and TEWL were measured.Then,all the mice were sacrificed,and skin tissue specimens were resected from the back of mice followed by histopathologic examination and immunohistochemical staining for the detection of filaggrin (FLG),involucrin (IVL) and kallikrein 7 (KLK7) expressions in the epidermis.Results After start of treatment,both the Qingpeng side and vehicle side showed significantly decreased TEWL on day 3 (t =2.651,2.996,respectively,both P＜ 0.05) and 7 (t =3.615,3.586,respectively,both P＜ 0.05),but increased water content of the stratum corneum on day 3 (t=9.029,5.830,respectively,both P＜ 0.001) and 7 (t =13.842,11.299,respectively,both P ＜ 0.001) compared with those before the treatment.However,no significant differences were observed in TEWL or the water content of the stratum corneum between the Qingpeng side and vehicle side at any of the time points (all P ＞ 0.05).In addition,there were no marked differences in mouse skin appearance,skinfold thickness or histopathologic manifestations between the vehicle group and model group,while the manifestations of dermatitis were attenuated to different extents in the three Qingpeng groups compared with the model group.Both TEWL and KLK7 expression levels were significantly decreased in the vehicle group and three Qingpeng groups compared with the model group (all P ＜ 0.05),but similar between the vehicle and Qingpeng groups (all P ＞ 0.05).Conclusion The vehicle of Qingpeng ointment can improve skin barrier function.

Objective To evaluate the efficacy and safety of maintenance treatment with Qingpeng ointment in the prevention of recurrence of localized eczema.Methods Totally,197 patients with localized eczema were topically treated with halometasone cream for 2-4 weeks.The patients who achieved clinical cure were then randomly classified into two groups to receive maintenance treatment with Qingpeng ointment (Qingpeng group,n =69) or vitamin E-containing cream (emollient group,n =68).Patients were followed up for 8 weeks for the detection of recurrence after the discontinuation of maintenance treatment.Results During the follow up,the recurrence rate in the Qingpeng group was significantly lower than that in the emollient group (19.70％ vs.37.31％,x2 =5.06,P ＜0.05).Significant differences were also observed in the duration of remission between the Qingpeng group and emollient group ((43.92 ± 15.70) vs.(32.60 ± 14.40) days,t =2.23,P＜ 0.05).The incidence rate of adverse reactions was 1.45％ in the Qingpeng group during the maintenance treatment,and no adverse reactions were observed in the emollient group.Conclusion The maintenance treatment with Qingpeng ointment can delay the recurrence of localized eczema to some extent with a favorable safety.

Objective To evaluate the efficacy and safety of Qingpeng ointment for the treatment of localized dermatitis and eczema in children.Methods Sixty-eight outpatient children with localized dermatitis or eczema were included in this study.All the patients were topically treated by Qingpeng ointment twice daily for a week,which was agreed by their patients.The efficacy was evaluated after the end of the treatment by lesion scores,and adverse reactions during the treatment were recorded.Results Of the 68 patients,61 completed the trial,7 withdrew from the trial because of worsened lesions,unfavorable effects and distrust of this drug.The total response rate was 81.4％ (31/51) in patients treated with Qingpeng ointment alone.Irritant reaction occured in only one case,but did not influence the treatment.Conclusion Qingpeng ointment displays a favorable efficacy and safety in the treatment of localized dermatitis and eczema in children.

Objective To study the induction of amelogenin in formation of hard tissues in the pulp tissues,to provide experimental foundation for research in formation of hard tissues induced with amelogenin.Methods Sixteen male Wistar rats were divided into control group and experimental group.Cells and matrix structures and amelogenin expression in the growing ends of cyclophosphamide(CP)-affected incisors were examined by histological and immunohistochemical methods.Results Experiment group:extensive edematous changes were noted in the apical pulp tissue of the incisor associated with disappearance of odontoblasts facing to presecretory and secretory ameloblasts. The cell layer of young ameloblasts was therefore lined directly with pulp tissue in which some osteodentin-like tissue was present near the ameloblasts. Immunoreactions for amelogenins were observed in the labial side of the pulp,particularly around the newly induced hard tissues in the affected region. Conclusion Amelogenins has induction in formation of hard tissues in dental pulp following the inhibition of formation of odontoblasts by CP.

Objective To analyse the clinical significance of LHRH exciting test in the differential diagnosis of constitutional delayed puberty (CDP) and hypogonadotropic hypogonadism (HH). Methods Eighty-one cases from 1982 to 1998 were investigated and followed up. They were all at genital stage Ⅰ. After injection of 100 ?g LHRH, the blood samples (3 ml) were taken at -15, 0, 15, 30, 45, 60, 90 and 120 min. The serum LH and FSH levels were determined by radioimmunoassay. Then they were followed up every 3-24 months. After they received LHRH exciting test, they were followed up until over 18 years old. According to their puberty development status, they were divided into 3 groups, normal group (n=34),CDP group (n=16) and HH group (n=31),andthemeanage,whenthey received LHRH exciting test, was (10.2?0.9, range 9-14) years, (16.0?1.0, range 14-18) years and (17.1?1.4, range 16-22) years respectively. Results There were no significant differences in serum LH baseline level and peak time in normal, CDP and HH groups, but the serum LH peak level, LH increment (peak LH level minus baseline LH level), LH increment ratio (peak level/baseline level of LH) and the area under LH curve (AUC LH ) of normal group were significantly higher than those of CDP group and HH group (all P

Objective: To investigate the clustering of health-risk behaviors and its influencing factors among children and adolescents in Yancheng City, Jiangsu Province, so as to provide insights into the prevention and control of health-risk behaviors among children and adolescents. Methods: Students were randomly sampled from 4 primary schools, 4 junior high schools and 4 senior high schools in Yancheng City using a multi-stage stratified cluster random sampling method from September to December 2021. Students' demographics and 12 health-risk factors including unhealthy diet, insufficient physical activity and attempted smoking were collected using the Student's Health Status and Influencing Factors Questionnaire by Jiangsu Provincial Center for Disease Control and Prevention, and factors affecting the clustering of health-risk behaviors were identified using a multivariable linear regression model. Results: A total of 2 925 valid questionnaires were recovered, and the respondents included 1 611 boys (55.08%) and 1 314 girls (44.92%). A total of 2 896 respondents were detected with health-risk behaviors, with a detection rate of 99.09%, and 2 772 respondents were detected with clustering of health-risk behaviors (93.06%). Insufficient sleep, insufficient physical activity and insufficient duration of outdoor activity were predominant patterns of clustering. The median number of health-risk behaviors was 4.00 (interquartile range, 2.00) per capita. Multivariable linear regression analysis showed that boys (β=0.232), grade (junior high school, β=0.519; senior high school, β=0.427), urban area (β=0.241), living at school (β=0.395), family structure (single parental family, β=0.188; other families, β=0.344) and father's education level of primary school and below (β=0.369) were factors affecting clustering of health-risk behavior among primary and high school students. Conclusions The detection of health-risk behaviors is high among children and adolescents in Yancheng City, and insufficient sleep, insufficient physical activity and insufficient duration of outdoor activity are predominant health-risk behaviors. Boys, junior high school and above, urban areas, living at schools, single parents, and fathers with a low educational level lead to a high degree of clustering of health-risk behaviors.