Objective To explore the molecular mechanism of programmed cell death 10(PDCD10)mediating glioma migration.Methods A PDCD10-silenced glioma cell line(U251)was constructed by siRNA,and the cells were treated with Okadaic Acid(OA),a protein phosphatase 2A(PP2A)phosphatase inhibitor,to investigate the effect of down-regulation of PDCD10 on the behavior of U251 cells;Western Blot(WB)was applied to detect the expression of PDCD10,PP2A,PP2Ac-307,p38 and pP38 in the control group and PDCD10 down-regulation group of U251 cells,to study the mechanism of PDCD10 regulation of PP2A/p38 signaling.Results Down-regulation of PDCD10 in U251 cells promoted the cells migration(P＜0.05),which could be inhibited by PP2A phosphatase inhibitor OA(P＜0.01).Further studies showed that down-regulation of PDCD10 promoted glioma cell migration via modulating the phosphorylation of PP2A,which in turn altered the activity of p38(P＜0.01).Conclusion PDCD10 may mediate glioma cell migration by regulating PP2A/p38 signaling.

Objective:To construct and validate a prediction model for the risk of intermediate cesarean delivery for primiparous women with failed vaginal trial of labor.Methods:Clinical data of 6 128 pregnant women who gave birth in the Affiliated Hospital of Jining Medical College from January 2019 to December 2020 were collected. The puerpera was randomly divided into train set ( n=4 290) and validation set ( n=1 838). The factors influencing the conversion to cesarean section in primiparous women with failed vaginal trial of labor were analyzed with univariate and binary multivariate logistic regression, and a risk prediction model was established based on the influencing factors. The predictive power of the model was assessed by receiver operating characteristic (ROC) curve and the Hosmer-Lemeshow goodness-of-fit test in train set and validation set. Results:Among 6 128 pregnant women 1 042 cases failed in vaginal trial of labor and were transferred to cesarean section. Univariate analysis showed age, occupation, gestational weight gain, days of gestation, body temperature before delivery, fetal heart condition at delivery, fetal abdominal circumference, Bishop score, premature rupture of membranes, gestational illness, mode of induction of labor, labor analgesia, and fetal orientation were significantly associated with converting to cesarean delivery (all P<0.05). The multivariate binary logistic regression analysis showed that the age, gestational weight gain, body temperature, gestational co-morbidities, days of gestation, premature rupture of membranes, amniotic fluid contamination, induction of labor, and abnormal occipital position were independent risk factors for intermediate cesarean delivery ( OR=1.03-8.06, all P<0.05); while height, occupation, Bishop score, and labor analgesia were protective factors for intermediate cesarean delivery ( OR=0.17-0.96, all P<0.05). A risk prediction model was constructed based on the risk factors and protective factors. In train set, the area under the ROC curve(AUC) of the model was 0.902 (95% CI: 0.89-0.92, P<0.001), with the best cutoff value of 0.138, the sensitivity and specificity were 0.837 and 0.825, respectively; and the Hosmer-Lemeshow goodness-of-fit test showed P=0.192. In validation set the AUC of the model was 0.917 (95% CI: 0.90-0.93, P<0.001), and the sensitivity and specificity were 0.826 and 0.851, respectively; the total correct rate of the model was 87.21% (1 603/1 838). Conclusion:The risk prediction model of failed vaginal trial of labor in primiparous women for intermediate cesarean delivery constructed in this study has good clinical prediction efficacy and high correctness rate.

Confusional arousal (CA) is a rare non-rapid eye movement sleep-related parasomnia and rarely reported in China, leading to misdiagnosis and mistreatment in clinic. A detailed collection of clinical symptoms and simultaneous video polysomnography is very important for diagnosis and differential diagnosis of CA. A elderly patient with CA was diagnosed according to the International classification of sleep disorders, third edition diagnostic criteria. The summary and analysis of the patient is conducted to improve the understanding of CA, meanwhile to avoid misdiagnosis and mistreatment.

Objective: To explore serum levels of brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF), and whether changes of BDNF and GDNF are correlated with sleep quality and cognitive function in patients with chronic insomnia disorder (CID). Methods: Fifty-seven CID patients in the Department of Sleep Disorders, Chaohu Hospital of Anhui Medical University and 30 healthy controls were enrolled from May 2017 to July 2018. Pittsburgh Sleep Quality Index (PSQI) was used to assess the degree of insomnia severity (some CID patients were monitored by overnight polysomnography). Montreal Cognitive Assessment (MoCA) scale and Nine-Box Maze were used to assess general cognitive function and specific memory function, respectively. The serum levels of BDNF and GDNF were detected using ELISA. Results: Compared to the controls, CID patients had significantly higher PSQI scores (CID patients: 14.0±2.2, healthy controls: 3.9±1.1; t=28.093, P<0.01), lower MoCA scores (CID patients: 24.5±3.6, healthy controls: 26.5±0.9; t=-2.985, P<0.01), more errors in object working memory (CID patients: 1.0 (0, 1.0), healthy controls: 0 (0, 0.3)), spatial working memory (CID patients: 3.0 (2.0, 4.0), healthy controls: 1.0 (1.0, 2.0)) and object recognition memory (CID patients: 0 (0, 0), healthy controls: 0 (0, 0); Z=-2.896、-5.007、-2.306, P<0.05), and lower serum BDNF (CID patients: (19.48±7.50) ng/ml, healthy controls: (46.49±13.33) ng/ml; t=-10.274, P<0.01) and GDNF (CID patients: (32.76±14.04) pg/ml, healthy controls: (59.63±20.30) pg/ml; t=-7.240, P<0.01). The partial correlation analysis showed that in the CID patients, the levels of BDNF and GDNF were correlated with PSQI scores negatively (r=-0.293, -0.320, P<0.05) and MoCA scores positively (r=0.331, 0.295, P<0.05). The BDNF level was also correlated with the duration of disease and the errors in the spatial working memory test negatively (r=-0.319, -0.393, P<0.05), and the GDNF level was correlated with the total sleep time detected with polysomnogram positively (r=0.520, P<0.05). Conclusion Serum BDNF and GDNF levels in CID patients were lower than those in healthy controls, and correlated with sleep quality and cognitive impairment.

Objective:To explore the changes of serum levels of copeptin and α-amylase and their correlations with sleep and cognition in patients with chronic insomnia (CI).Methods:From September 1, 2018 to May 31, 2019, fifty CI outpatients or inpatients from the Department of Sleep Disorder, Affiliated Chaohu Hospital of Anhui Medical University, were enrolled continuously, and thirty good sleepers from the Physical Examination Center of the hospital, were also enrolled to serve as controls. Pittsburgh Sleep Quality Index (PSQI), polysomnography (PSG) and Pre-Sleep Arousal Scale (PSAS) were used to assess the insomnia severity and sleep disorder susceptibility. Montreal Cognitive Assessment scale (MoCA) and Nine-Box Maze were used to respectively assess general cognition and memories. The serum levels of copeptin and α-amylase were detected using enzyme linked immunosorbent assay.Results:Compared to the controls, the CI patients had increased PSQI score (16.0 (15.0, 17.0) vs 4.0 (2.8, 6.0); Z=-7.678, P<0.001) and PSAS score (33.0 (30.0, 37.5) vs 17.0 (16.0, 18.5); Z=-7.350, P<0.001), decreased MoCA score (24.1±2.5 vs 26.7±1.9, t=-4.625, P<0.001), increased numbers of errors in the object working (1.0 (0, 1.0) vs 0 (0, 1.0), Z=-2.099, P=0.036), spatial working (2.0 (1.0, 4.0) vs 1.0 (0, 2.0), Z=-3.935, P<0.001) and object recognition (1.0 (0, 2.0) vs 0 (0, 0), Z=-2.266, P=0.023) memories, and elevated serum levels of copeptin ((35.1±19.9) pg/ml vs (14.8±6.9) pg/ml, t=5.414, P<0.001) and α-amylase ((990.1±193.7) U/L vs (728.9±230.5) U/L, t=5.597, P<0.001). In the CI patients, the level of copeptin was positively correlated with PSQI score ( r=0.338, P=0.013), PSAS score ( r=0.316, P=0.021), sleep latency ( r=0.324, P=0.018), number of awake ( r=0.325, P=0.017) and stage 1 percent of non-rapid eye movement sleep ( r=0.278, P=0.044), and negatively correlated with stage 2 percent of non-rapid eye movement sleep ( r=-0.279, P=0.043); α-amylase was positively correlated with numbers of awake in PSG ( r=0.293, P=0.033). Multiple linear regression analysis showed that copeptin level affected PSQI score (β=0.255, P=0.043) and sleep latency (β=0.254, P=0.043). Conclusion:The levels of copeptin and α-amylase in CI patients elevate, and copeptin may be associated with initial sleep difficulties, but not with cognitive ability, in patients with CI.

OBJECTIVE To varify the difference of serum specific IgE levels in different ages and gender inpatients with Aspergillus fumigatus or Alternaria alternate induced allergic rhinitis. METHODS In this study, 85 patients with Aspergillus fumigatus or Alternaria alternate induced allergic rhinitis were included. sIgE tests was done by Immuno CAP 250TM System(Thermo Fisher Scientific, Uppsala, Sweden). RESULTS Mean level of aspergillus fumigatus sIgE was significantly lower than Alternaria alternate(P<0.05), single or dual fungi allergy show no impact on this difference. sIgE level showed positive correlation with age in Aspergillus fumigatus group(P <0.05), while negative in Alternaria alternate group(P <0.05). CONCLUSION Aspergillus fumigatus and Alternaria alternate are mostly common fungi allergens, sIgE detection of those two fungi are important for diagnosis of fungi induced allergic rhinitis and other respiratory diseases in people with different ages.

Objective To explore serum level of 25-hydroxy vitamin D3 (25 [OH]D3) and its correlations with sleep quality and cognitive function in patients with chronic insomnia disorder (CID).Methods Fifty CID patients,admitted to our hospital from October 1,2016 to September 1,2018,and 40 healthy controls were enrolled.Pittsburgh Sleep Quality Index (PSQI) was used to assess insomnia severity (objective sleep status being assessed using polysomnography throughout a night in some patients).Montreal Cognitive Assessment scale (MoCA) was used to evaluate the general cognitive function.Serum level of25(OH)D3 was detected by ELISA.Results As compared with the controls,CID patients had significantly higher PSQI scores (P＜0.05) and statistically higher Hamilton's Depression Scale-17 scores (P＜0.05).The CID patients had significantly lower MoCA scores,having more errors in object work,spatial work and object recognition memorizes in Nine-Box Maze Test than the controls.Partial correlation analysis showed that in CID patients,the level of 25(OH)D3 was negatively correlated with PSQI scores (r=-0.320,P=0.030),spatial working memory errors (r=-0.300,P=0.043) and disease course (r=-0.360,P=0.014),whereas it was positively correlated with total sleep time (r=0.515,P=0.014)and MoCA scores (r=0.422,P=0.003).Conclusion The level of 25 (OH)D3 decreases in the CID patients,which is correlated with insomnia severity and course,and may affect the cognitive function.

Objective To explore the impact of hypothyroidism on left ventricular ( LV ) mechanics using two-dimensional speckle tracking imaging ( 2D-STI ) . Methods Forty-two patients with newly diagnosed overt hypothyroidism ( HT ) were prospectively collected as the case group and 47 subjects matching by age ,gender and hypertension history were enrolled as control group . All participants received comprehensive echocardiography examination ,and 2D-STI was used to assess LV global longitudinal strain ( GLS) and mechanical dispersion ( MD ) . The inter-group difference in GLS and MD ,correlations with thyroid hormones and reproducibility were evaluated . Results LV GLS at both sectional ( apical 4- ,3- ,2-chamber views) and global levels were significantly impaired in the HT group than the control group [ ( - 21 .3 ± 3 .2) ％ vs ( - 23 .9 ± 2 .9) ％ ,( - 20 .4 ± 3 .8 ) ％ vs ( - 22 .7 ± 2 .9 ) ％ ,( - 21 .2 ± 3 .9) ％ vs ( - 23 .9 ± 2 .5) ％ ,( - 20 .9 ± 3 .4) ％ vs ( - 23 .5 ± 2 .3) ％ ;all P < 0 .01] . Similarly ,MD at both sectional ( apical 4- ,3- ,2-chamber views) and global levels were significantly prolonged in the HT group than the control group[ 3 .4 ms vs 0 .9 ms ,2 .2 ms vs 0 .7 ms ,2 .3 ms vs 1 .7 ms and 12 .2 ms vs ( 5 .9 ± 2 .6) ms ;all P < 0 .01] . No significant correlation was found between MD and GLS ,left ventricular ejection fraction ( LVEF) ( r = 0 .12 , P = 0 .27 ; r = - 0 .17 , P = 0 .10) . Weak correlations were found between MD and FT3 ,FT4 ,TSH( r = - 0 .34 , P = 0 .01 ; r = - 0 .38 , P = 0 .005 ; r = 0 .31 , P = 0 .02) . Conclusions Primary overt HT is associated with impaired LV deformation and increased systolic dyssynchrony . LV MD is a promising parameter for assessment of myocardial impairment in HT .

Objective To revise the attitudes toward seeking professional psychological help scaleshort form(ATSPPH-SF) and to exam the reliability and validity of ATSPPH-SF Chinese revision.Methods Based on studying and analyzing foreign related questionnaires,1 657 adults from Tianjin were chosen as subjects to exam the reliability and validity of ATSPPH-SF Chinese revision.Results The three-dimensional structure were obtained according to the results of exploratory factor analysis,and the total variance explained amounted to 54.03％.The results of Confirmatory factor analysis showed that the measurement model fit the data well (x2/df=2.94,RMSEA =0.04,CFI =0.97,GFI =0.98,SRMR =0.01,AGFI =0.97,TLI =0.95,IFI =0.97,NFI =0.96).The cronbach's α coefficients of the questionnaire degreed from.0.71 to 0.78.The retest reliability of the questionnaire degreed from 0.65 to 0.82.Correlation between each item and the total score ranged from 0.32 to 0.63 and the correlations between each dimension and total questionnaire were from 0.67 to 0.82.There was a significant positive correlation with ATSPPH(r=0.67,P＜0.01).Conclusion The revised ATSPPH-SF has good reliability and validity,which can satisfy the domestic demand for relevant research and application.

Objective:To investigate the risk factors of central lymph node metastasis (CLNM) and lateral neck lymph node me-tastasis in papillary thyroid microcarcinoma (PTMC) patients, and to analyze the importance of high resolution ultrasonography in the diagnosis of lateral neck lymph node metastasis in PTMC patients. Methods:A retrospective protocol was applied, and a total of 1 037 PTMC patients were reviewed. These patients underwent central lymph node dissection or thyroidectomy with lateral neck lymph node dissection between January and November in 2013 in the Tianjin Medical University Cancer Institute and Hospital. Clinicopathological factors, namely, age, sex, primary tumor size, multifocality, bilateralism, thyroid capsular invasion, and local invasion, were analyzed. Results: CLNMs were found in 332 of 1037 patients (32.0%), and 71 out of 1037 patients had lateral neck lymph node metastasis (6.85%). In the univariate analysis, patients with the following risk factors were at high risk of CLNM (P<0.05):male, aged≤45 years old, with primary tumor size of>5 mm, multifocality, bilateralism, thyroid capsular invasion, and local invasion. Male patients with cen-tral lymph node metastasis positively showed high lateral neck lymph node metastasis rate (P<0.05) according to high-resolution ultra-sonography diagnosis. The rate of lateral neck lymph node metastasis increased with increasing number of central lymph node metasta-ses. The sensitivity and specificity of high resolution ultrasonography for lateral neck lymph node metastasis were 92.96%and 81.48%in PTMC patients.Conclusion:Prophylactic central compartment lymph node dissection needs to be performed in patients with CLNM risk factors (i.e., male, aged≤45 years old, primary tumor size of>5 mm, multifocality, bilateralism, thyroid capsular invasion, and lo-cal invasion). The importance of high-resolution ultrasonography in diagnosing lateral neck lymph node metastasis was revealed by the results. Thus, this method should be widely popularized. Radical neck dissection should be performed in male patients who received a positive diagnosis via ultrasonography or those with PTMC who had more than three positive nodes in the central lymph node metasta-sis. However, given the high occurrence rate of PTMC, a prospective study needs to be conducted in the future.