BACKGROUND:5-Fluorouracil is a common chemotherapy drug for gastric cancer, but it is more likely to develop drug resistance in clinical treatment. Studies have shown that tumor stem cels are lowly sensitive to chemotherapeutic drugs, which may be an important cause of chemotherapy resistance. OBJECTIVE:To analyze the sensitivity of gastric cancer stem cels to 5-fluorouracil in vitro, and to understand the mechanism of drug resistance associated with gastric cancer. METHODS:Based on the sorting strategy, human gastric cancer cel clones were isolated from AGS cel lines. CD44 and thymidylate synthetase expression in different clones was detected using immunocytochemistry analysis. Clone formation assay was used to evaluate self-renewal capacity of different clones. Cel counting kit-8 was used to determine the clonal growth inhibition rate of AGS under treatment with different concentrations of 5-fluorouracil. RESULTS AND CONCLUSION:The AGS cels that were inoculated with low density and cultured could differentiate into 32 forms, including the ful clone (16%, 5/32), the second clone (66%, 21/32) and the accessory clone (19%, 6/32). Among them, the ful clones highly expressed CD44 and thymidylate synthetase, and could generate a great amount of passage 2 clones after inoculation; the second clones showed a weak expression of CD44 and thymidylate synthetase, and could generate a smal number of passage 2 clones after inoculation; the accessory clones showed a weak or no expression of CD44 and thymidylate synthetase, and there was no passage 2 clone after inoculation. Under the effect of different concentrations of 5-fluorouracil, the growth inhibition rates of the secondary clones and AGS cels were both higher than that of the ful clones (bothP < 0.05). These findings indicate that gastric cancer stem cels have a relatively lower sensitivity to 5-fluorouracilin vitro, which is speculated to be an important mechanism of drug resistance.

Objective To investigate the incidence of unilateral spatial neglect(USN)after brain injury in different area with varied test,and to survey their sensitivity.Methods 196 patients after brain injury were assessed with varied test to identify USN.Results The total incidence of USN was 41.33%,which was the highest in the patients with parietal lobe lesion and less in the boundary of tempo-parietal-occipital lobe,the temporal lobe and basal ganglion injury in turn.The sensitivity of the Albert test was the highest,which was less of Shenckenberg test,letter-figure-cancellation test,copying drawing test,clock-drawing test and character test in turn.However,the sensitivity of Albert test was the highest in the patients with frontal lobe lesion,and that of Shenckenberg test was the highest when the temporal,parietal lobe or the boundary of tempo-parietal-occipital lobe injured.Conclusion The incidence of USN was high after brain injury.It is important to choose the test tool according to the area of the focus.

Objective:To construct of transfectant cells expressing ??TCR with specific CDR3 sequence.Methods:Specific CDR3 region sequence of DBS4.3,a known ??T cell clone,was inserted into ?9 and ?2 chain to substitute original CDR3 sequence using overlapping PCR method.After the full-length ?9 and ?2 chains were ligated with expression vector pREP7and pREP9 respectively,they were co-trasfected into a cell line of human Jurkat cells with TCR ? chain gene-defective mutant J.RT3-T3.5.When the transfectant cells expressing specific ??TCR were stimulated by antigen,production of IL-2 was detected by ELISA and Realtime PCR.Results:By ELISA and Realtime PCR,it was exhibited that the transfectant cells expressing DBS4.3 specific ??TCR secreted IL-2 under stimulation of iso-butylamine and anti ??TCR antibody.Conclusion:The transfectant Jurkat cells expressing ??TCR with specific CDR3 sequence are successfully constructed.It provides a platform for the research of recognition mechanism of ??TCR.

To cultivate nursing students with international professional ability for higher vocational education is the key to promote the internationalization of Chinese nursing field. Through analyzing the de-veloping trends and problems of international nursing education in China, based on the international nurses' ability, and study from the nurses training model of Philippines, the international nursing training model was constructed. The paper discussed the selection and retention of students, emphasizing the cultivation of students' international nursing concept and professional English, focusing on the ISPN curriculum and the integration of theory and practice, encouraging the cooperation of international school and faculties sharing, adapting the students-centered teaching methods, and establishing a corresponding sound evaluation system.

The factors affecting cerebral ischemia model of rat established by middle cerebral artery occlusion include animal selection, drugs of anesthesia, surgical site, thread affect building, and etc. To find and manufacture an appropriate thread is the key to success. It includes the species, length, diameter, hardness, and placing method of the thread. The improvement of these factors greatly increases the successful rate in the establishment of focal cerebra ischemia model of rat caused by middle cerebral artery occlusion.

Objective To observe the effect of comprehensive rehabilitation on POEMS syndrome during sequel period. Methods Two cases of POEMS syndrome with multiple peripheral nerve injury as the main manifestation received physical therapy, occupational therapy, acupuncture, surface electromyography biofeedback and other comprehensive rehabilitation treatment. Results After treatment, the muscle strength, trunk balance, standing balance ability, activities of daily living (ADL) improved. The score of Berg Balance Scale increased from 18 to 28 in the first case, the modified Bathel Index increased from 55 to 70 in the second case. Conclusion Comprehensive rehabilitation can improve the function of patients with POEMS syndrome during sequel period, and surface electromyography biofeedback plays an im-portant role.

Objective To analyze the features of normal high-frequency ultrasonography of tarsal tunnel. Methods Forty volunteers (20 males and 20 females) were examined with high-frequency ultrasound (12 MHz). The tendons,nerve and blood vessels in tarsal tunnel were observed from short-axis and long-axis views dynamically. The areas of tarsal tunnel and posterior tibial nerve were measured and com-pared between the males and females. Results High-frequency ultrasonography depicted the anatomical structure of tarsal tunnel,and the tendons,nerve and blood vessels presented different sonographic features that were easy to differentiate. The area of tarsal tunnel: male (7.61 ±1.00) cm2,female (6. 61 ± 1. 07) cm2 (P <0. 01). The area of posterior tibial nerve: male (9. 59 ± 0. 75) mm2,female (8.91 ±0.74) mm2(P<0.01). Conclusion High-frequency ultrasonography can clearly show and accurately measure the tarsal tunnel structure. To be familiar with the normal ultrasonographic anatomy of tarsal tunnel will help to improve the efficacy of ultrasound diagnosis of tarsal tunnel abnormalities.

Objective To investigate the unilateral spatial neglect (USN) on the rehabilitation of hemiplegia after brain injury.Methods 35 patients with USN and 38 patients without USN were selected and divided into two groups (USN group and without USN group). All patients of two groups accepted integrative treatments mainly with Bobath technique by professional therapists. The function of movement, balance, ambulation, hand and activities of daily living (ADL) were all particularly assessed by simplified Fugl-Meyer movement assessment, Fugl-Meyer balance assessment, Holden functional ambulation classification, hand function classification and Modified Barthel Index (MBI) before treatment and 1 and 2 months after treatment.Results After treatment, the scores of the Fugl-Meyer movement assessment, Fugl-meyer balance assessment and MBI of all patients in two groups significantly improved ( P<0.001). But there was a significant difference between the two groups in scores of balance function, MBI, hand function (all P<0.05), and the Holden functional ambulation classification ( P<0.001).Conclusion USN induced by brain injury has obviously effects on rehabilitation of motor, balance, ambulation and ADL of hemiplegia.

To explore the current status of children receiving primary health services at a community health service center in Shenzhen so as to provide references for the formulation of children health care policies.According to the patient electronic data in 2011,the clinical profiles were analyzed for children aged under 7 years seeking the advice of general practitioners (GPs) at Niuhu Community Health Service Center.Children aged under 7 years accounted for 12.4％ of all subjects.The causes of seeing the GPs were as follows:acute upper respiratory.infection(58.2％),acute bronchitis(14.9％),gastroenteritis(5.6％),trauma(3.3％)and dermatitis(3.2％).And no difference existed in the workload between pediatrists and surgeons,internists or herbalists.The common and frequent community diseases may be diagnosed and managed at a community health service center.

PurposePulmonary tuberculosis (PTB) in the patients with AIDS often manifest as an atypical clinical manifestations, less positive rate in lab and various imaging features. This paper aims to compare the clinical and imaging manifestations of PTB in patients with AIDS and in patients with normal immunity may help us to improve the understanding of PTB in AIDS.Materials and MethodsThe clinical manifestations, the lab results and chest imaging data of 142 conifrmed PTB in patients with AIDS (observation group) and 150 conifrmed PTB in patients with normal immunity (control group) were retrospectively analyzed, the imaging review included the site, shape, density, property and the enhancement patterns of the enlarged lymph node in mediastinum and the status of pleura.ResultsThe incidences of fever, dyspnea, asthenia, anorexia, loss of weight, diarrhea, blurred vision, urinary irritation, oral leukoplakia, pruritus, rash, other pulmonary infections and extrapulmonary tuberculosis in observation group were signiifcantly higher than those in control group (χ2=4.173-68.542,P<0.05,P<0.01,P<0.001). The positive rates of tuberculosis in cough, hemoptysis, putum smear and sputum culture in observation group were signiifcantly lower than those in control group (χ2=30.376, 9.254 and 16.200, P<0.05). Imaging manifestations: observation group of type I, type II, type IV and type V patients were more than those in the control group (χ2=5.977, 8.621, 18.769 and 20.864, P<0.05). The lesions located at middle and lower lobes and involved more than three lobes in observation group than in control group at the same time (χ2=18.692 and 5.860,P<0.05, P<0.001), military nodules, enlarged lymph nodes in mediastinum and pleural effusion appeared more commonly in observation group than in control group (χ2=13.404, 34.704 and 19.469,P<0.001), while calcification, fibrosis and cavity were less in observation group compared to control group (χ2=6.539, 4.658 and 7.280,P<0.05,P<0.01,P<0.001). ConclusionPTB should be considered when AIDS patients present multiple symptoms in whole body, with pulmonary lesions located at middle and lower lobes & involved more than three lobes, military nodules, mediastinal enlarged lymph nodes and pleural effusion.