Aim To evaluate the effect and the mechanism of valdecoxib on Lewis cancer. Methods Western blot was used to detect the expression of VEGF, MMP-2, Bax, Bcl-2 and Caspase-3 in tumor tissue. Flow cytometry was used to observe the effect of valdecoxib on proliferation, apoptosis and the cell cycle distribution. MTT assay was used to observe the lymphocyte transformation rate. Results ① Valdecoxib inhibited the growth of the tumor and increased the survival rate. ② 10~40 mg?kg -1?d -1 Valdecoxib increased the apoptosis rate from 19.1% in control group to 23.1%~29.1%, and did not affect the distribution of cell cycle. ③ The expression level of Bcl-2 was decreased and expression levels of Bax, COX-2, MMP-2, Caspase-3 and VEGF were not affected. ④ Valdecoxib did not affect the weight, the lymphocyte transformation rate, spleen index, and thyrus index of the mice. Conclusion The inhibitory effect of valdecoxib on Lewis tumor is associated with apoptosis.

This paper summarizes professor LU Zhi-zheng’s clinical experience of treating thoracic obstruction from regulating spleen and stomach.Seven kinds of syndromes and treatments are concluded and analyzed.Proved cases were included in order to provide reference for the clinical treatment of thoracic obstruction.

Objective To investigate the relativity for weight of macrosomia predicted by ultrasound and clinical measurements . Methods 1 037 subjects who were delivered in May to November 2012 ,in Chongqing Health Center for Women and Children ,were collected and analyzed satistically .The parameters for the comparisons included maternal abdominal circumference (MAC) ,fundal height(FH) ,and fetal diameters measured by ultrasonic imaging such as biparietal diameter (BPD) ,head circumference(HC) ,femur length(FL) ,and abdominal circumference(AC) .The coincidence was calculated with real weights and compared with clinical predic-tions .Results When FH+ MAC 134 cm ,the accuracy of preliminary screening for macrosomia is the highest ,the area under the ROC curve is 0 .895 .Combining with the abdominal circumference(AC) by prenatal ultrasound ,the highest prediction rate of mac-rosomia is 75 .2% .when Fetal AC≥37 cm ,macrosomia incidence rate took 91 .7% .Conclusion Pregnant women with FH+MAC<134 cm ,whose incidence of Macrosomia is low .While if the FH+MAC≥134 cm ,combining with the prenatal ultrasound whose fetal AC>36 cm ,especially ≥37 cm ,the possibility of macrosomia is very high .

Objective To explore a new type community nursing mode which can improve the life quality of community elderly population with depression tendency.Methods In the chosen community of Jilin City,we used Geriatric Depression Scale (GDS) to identify the elderly population with depression tendency.78 elderly people with depression tendency who met the conditions of study were assigned to the intervention group and the control group with 39 people in each group randomly.8-week group-intervention was given to the intervention group,and no intervention was given to the control group.For the intervention group,the total intervention time was 8 weeks,once a week for continuous 8 times together and intervention time was 90 minutes for each time.The Geriatric Depression Scale (GDS) and the world health organization quality of life (WHOQOL-BREF) were used to measure the quality of life of the two groups.Results After intervention,the total scores of the intervention group were significantly lower than those of the control group,the difference was statistically significant.Average scores of each dimension in the life quality of the intervention group were obviously higher than those of the control group.Conclusions Group intervention mode for the community elderly population with depression tendency was feasible,effective,and reasonable.It can be applied to mental health care of community elderly people,and can also provides basis for early discovery and early intervention of the community elderly people with depression.

Objective: To explore the impact of body mass index (BMI) on long-term prognosis in patients of acute ST-segment elevation myocardial infarction (STEMI) after percutaneous coronary intervention (PCI). Methods: A total of 1435 consecutive STEMI patients received PCI in our hospital from 2013-01 to 2013-12 were enrolled. Based BMI (kg/m2), the patients were divided into 3 groups: Normal weight group, the patients with 18.5≤BMI<24.0, n=365, Overweight group, 24.0≤BMI<28.0, n=718 and Obese group, BMI≥28.0, n=352. The impact of BMI on major adverse cardiovascular and cerebral events (MACCE) was observed; weather BMI had predictive value for all-cause mortality and cardiac death was analyzed. Results: All-cause mortality in Obese group was lower than Normal weight group (0.6% vs 3.0%), P=0.027; while the incidences of bleeding, stroke, in-stent thrombosis, blood revascularization, re-myocardial infarction and cardiac death were similar among 3 groups. Multivariate analysis revealed that obesity was an independent predictor for all-cause death (HR=0.201, 95% CI 0.043-0.943, P=0.042), BMI was not the independent predictor for cardiac death. Conclusion: For STEMI patients after PCI treatment, the individuals with obesity had the better prognosis than those with normal weight and overweight. Obesity was an independent predictor for all-cause death and obesity paradox was applicable in such population.

OBJECTIVETo analyze the current situation about Glycyrrhiza resources, summarize the research achievements on Glycyrrhiza relative industries, and indentify the importance of developing Glycyrrhiza industries to promote the three-dimensional rural economic development in the Three-North areas.

METHODLiteratures and documents were referred to, in combination with overview and analysis of the outcomes and achievements of our group.

RESULT AND CONCLUSIONThe gap between supply and demand of resources is enlarged continuously. To develop high-quality Glycyrrhiza cultivation is the most powerful and effective measure to protect wild resources and the environment and promote the three-dimensional rural economic development in the Three-North areas.

China ; Drugs, Chinese Herbal ; economics ; Economic Development ; Glycyrrhiza ; Social Planning

To explore the relationship between blood level of big endothelin and contrast-induced acute kidney injury (CI-AKI) in patients with emergent percutaneous coronary intervention (PCI). Methods: A total of 1061 consecutive patients received emergent PCI in our hospital from 2013-01 to 2015-06 were enrolled. According to blood levels of big endothelin, the patients were divided into 2 groups: Normal big endothelin group, n=236 and Elevated big endothelin group, n=825. The baseline condition, procedural features, occurrence rate of CI-AKI and composite endpoint events at 6 and 12 months post-operation were studied which including nonfatal myocardial infarction, revascularization, stroke and all-cause death. The risk factors for CI-AKI occurrence were identified by Logistic analysis. Results: The overall occurrence rate of CI-AKI was 22.7% (241/1061). Compared with Normal big endothelin group, Elevated big endothelin group had the higher incidence of CI-AKI, increased composite endpoint events at 6 and 12 months post-operation with P=0.041, P=0.040 and P=0.021, respectively. With adjusted covariates, elevated blood level of big endothelin, no matter as a continuous variable or categorical variable had the enhanced risk of CI-AKI incidence in patients after emergent PCI. Conclusion: Elevated blood level of big endothelin may significantly increase the risk of CI-AKI in patients with emergent PCI.

Glycyrrhiza Radix is a commonly used Chinese herbal medicine, derived from the dried roots and rhizomes of Glycyrrhiza uralensis, G. glabra and G. inflata. The main bioactive constituents of licorice are triterpene saponins and flavonoids. There are various types of pharmacological properties of licorice are proved, including antiulcer, antiinflammation, spasmolysis, antioxidative, contravariance, antiviral, anticancer activities, hepatoprotective, eliminating phlegm and reinforcing remembrance effects. Triterpene saponins are the main components of Glycyrrhiza Radix and its pharmacological activities are comparatively thorough and clear. In recent years, licorice flavonoids, proved to have a variety of pharmacological activities, become one of the hot of pharmacological studies. In this review, we summarized the latest five-year progress in pharmacological activities of licorice extract, its triterpenoid saponins and flavonoids, and experimental researches indicated that licorice had both accommodation and protection activity on the digestive system, respiratory system, nervous system, and endocrine system, etc.
Animals ; Antioxidants ; analysis ; pharmacology ; Drugs, Chinese Herbal ; analysis ; pharmacology ; Flavonoids ; analysis ; pharmacology ; Glycyrrhiza ; chemistry ; Humans ; Triterpenes ; analysis ; pharmacology

OBJECTIVETo develop a HPLC method for the quantitative determination of four nitrogenous compounds in Pinellia peda-tisecta.

METHODThe determination was carried out on Diamonsil-C18 column(4. 6 mm x 250 mm, 5 microm) eluted with acetonitrile-water-triethylamine in gradient elution acetonitrile-water- triethylamine. The mobile phase was a flow rate of 1.0 mL x min(-1) and detection wavelength as 254 nm.

RESULTThe linear ranges of trigonelline, thymine, amidopurine and hypoxanthine were 0.2 - 1.0 microg (r = 0.999 6), 2.6-13 microg (r = 0.999 8), 0.55-2.75 microg (r = 0.999 9), 0.65-3.25 microg (r = 0.999 8), respectively. The average recoveries of trigonelline, thymine, amidopurine and hypoxanthine were 100.6%, 99.89%,100.5% and 100.0% respectively, and the RSD of the above four compounds were 2.4%, 0.20%, 1.1%, 1.4%, respectively.

CONCLUSIONThis method is reliable, accurate and specific. It can be used for quality control of the tube of P. peda-tisecta.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Nitrogen Compounds ; analysis ; isolation & purification ; Pinellia ; chemistry

Infectious diarrhea is a gastrointestinal infectious disease caused by a wide range of pathogens and found throughout the world. It is one of the most important public health problems in the world and the second leading cause of death among children under five years of age. The pathogens of infectious diarrhea include viral diarrhea pathogens, bacterial diarrhea pathogens, and parasites. Viruses are the most frequent pathogens, mainly including norovirus, rotavirus, astrovirus and sapovirus. The most frequently identified organisms causing bacterial diarrhea are diarrheagenic Escherichia coli, Salmonella, Shigella, Vibrio parahaemolyticus and Campylobacter. This paper provides an overview of the epidemiological trends and changes in the pathogen spectrum of infectious diarrhea for better understanding the distribution and epidemiological features of infectious diarrhea in China, and hopes to provide reference for developing prevention and control strategies and reducing the disease burden.