Objective To explore the clinical effect of disposable root canal treatment for elderly patients with chronic pulpitis.Methods 84 patients with chronic pulpitis were selected and randomly divided into the control group and observation group,each group in 42 cases(42 teeth).The control group was used multiple root canal treat-ment,and the observation group was used disposable root canal treatment,and following-up for 1 year after treatment, the clinical effect and success rates after one year were compared between the two groups.Results The total effective rate of the observation group was 92.9%, which was significantly higher than 69.0% of the control group (χ2 =3.860,P<0.05);After 1 years treatment,the total success rate of the observation group was 83.3%,which was sig-nificantly higher than 54.8%of the control group(χ2 =4.011,P<0.05).Conclusion The disposable root canal treatment for elderly patients with chronic pulpitis has a total effective rate,assembly and high power,which has cer-tain clinical reference.

OBJECTIVE:To provide reference for improving the utilization ratio of emergency contraceptive(EC)pills and re-ducing unwanted pregnancy. METHODS:Among 458 women with unwanted pregnancy in our hospital,self-designed question-naires were used to survey the respondents'knowledge and utilization of EC pills,reasons for not using,sources of EC knowledge and drug availability. The results were analyzed statistically. RESULTS:Totally 458 questionnaires were distributed and then 426 valid questionnaires were collected with effective rate of 93.0%. Among respondents,the awareness rate of EC was 81.2%(346/426),and the rate of those receiving higher education was higher than that of other education levels(P<0.001). 56.3%(240/426) of the respondents had ever used EC pills at one time,the utilization ratio of EC pills were higher in women less than 30-year-old and receiving higher education than other age and educational levels(P<0.01). Only 20.2%(86/426)of the respondents ever used EC pills after the last menstrual period and only 3.5%(3/86) of them took EC pills correctly. Not understanding or knowing EC was the main reason for not using EC pills,accounting for 60.6%(206/340). The highest proportion of source which the respon-dents got EC knowledge was network,accounting for 53.5%(185/346). 37.0%(81/219) of the respondents who had ever pur-chased drugs couldn't purchase EC pills conveniently. CONCLUSIONS:The utilization ratio of EC pills in women with unwanted pregnancy needs to be improved and non-standard drug use is more common. It indicates that health education of contraception still need to be strengthened and the availability of EC pills improved.

Neonatal sepsis progresses very fast,and its clinical signs are non-specific,and it can cause severe complications,such as shock,MODS.Therefore,early diagnosis is key to reduce mortality and improve prognosis.The clinical indicators in diagnosis of neonatal sepsis includ bacterial culture,peripheral hemogram,acute phase reaction proteins,cytokines,cell surface antigen and bacterial gene detection.This article reviews the clinical significance of these indicators in the early diagnosis,condition assessment,treatment monitoring of neonatal sepsis.

IL-18 was discovered as a proinflammatoty cytokine,stimulates natural killer and T cells and enhances Th1/Th2 immune response, and plays a central role in inflammation and immune response.. Immune-stimulating effects of IL-18 as a adjuvent agent against cancer,have antineoplastic properi-ties. However, Higher expression or secretion level of IL- 18 is detected in the blood of the majority of cancer pa-tients with poor prongnosis. IL-18 has also been associated with cancer progression and immune escape,is able to induce angiogenesis, metastasis. The dual effects of IL-18 in cancer need to be investigated further.

Objective To evaluate the distribution and expression of peroxisome proliferator activated receptor ?(PPAR?) in pituitary ACTH-secreting adenomas of human.Methods Immunohistochemistry and Western blot were used to identify the distribution and expression of PPAR?.Results PPAR? expressed in pituitary ACTH-secreting adenomas and normal pituitary tissues.PPAR? immunoreactivity was found in nucleoli of pituitary ACTH-secreting adenoma and normal pituitary cells.The expression in pituitary ACTH-secreting adenomas was significantly higher than that in normal pituitary tissues.Conclusion The expression of PPAR? in pituitary ACTH-secreting adenomas significantly increased,so it maybe a potential therapeutic target of pituitary ACTH-secreting adenoma.

Objective To optimize the expression conditions(expression and induction conditions)and purification methods(non-denaturing and denaturing purification)of recombinant Hq001 protein in salivary glands of Haemaphysalis qinghaiensis.Methods The recombinant plasmid pET-30a-Hq001 was transformed into competent cells E.coil BL21(DE3),E.coil Rosetta(DE3)and E.coil ArcticExpress(DE3)pRARE2 respectively for the selection of an optimal expression strain.The final concentration of IPTG(0,0.5,1.0 mmol/L),induction temperature(20,25 ℃)and induction time(0,2,4,6,8 h)were optimized.The recombinant bacteria expressed under the ideal induction condition were homogenized by French press and the target protein was purified by passing through a Ni-NTA affinity chromatography column under either native(denaturationrenaturation-column chromatography)or denatured conditions(denaturation-column chromatography-renaturation).The purified products were analyzed by 12% SDS-PAGE.Results E.coil BL21(DE3)was proved to be the most suitable strain for the expression of recombinant Hq001 protein.The optimum induction condition was induction with 0.5 mmol/L IPTG for 4 h at 25 ℃.The target protein with a relative molecular mass of approximately 18 800 was obtained by non-denaturing purification method,and the size was consistent with the expectation.Conclusion The recombinant protein rHq001 in salivary glands of Haemaphysalis qinghaiensis can be obtained by the optimized expression conditions and purification methods.

Thirty-eight cases with hysteromyoma were treated with Hai-Kun decoction. The effective rate which hysteromyoma was eliminated and reduced was 52. 78%. The cure effect was better than control groupand there was a significant difference (P

Objective To evaluate the effect of trimetazidine in ischemic cardiomyopathy patients with impaired systolic function. Methods There were 90 cases in total, and were divided into two groups randomly: 42patients took conventional therapy,and 48 patients took trimetazidine additionally. Plasma levels of brain natriuretic peptide (BNP), left ventricular ejection fraction(LVEF) and left ventricular end-diastolic dimension(LVEDD) were measured before and after trimetazidine therapy at 6 months, 12 months. Results Compared to conventional therapy group, LVEF, LVEDD and BNP were significantly improved in the trimetazidine group (P ＜ 0.01 ). Conclusions Trimetazidine additional to conventional therapy might improve heart function and reverse ventricles reconstitution in ischemic cardiomyopathy patients with impaired systolic function.

Objective To investigate the status of high-risk human papilloma virus(HR-HPV)infection and natural clearance in males whose wife have been infected,and to analysis the coincidence rates of HPV subtypes in couples.Methods Urethra epithelial cells and urethral secretions of 246 males whose wife infected with HR-HPV have been enrolled in the study to detect 13 kinds of HPV subtypes and redetect after 1 year.Results The natural clearance rate of HR-HPV in male was 31.82％(35/110).The infection rate was 44.72％(110/246)in initial detection,while 30.49％(75/246) in redetection,the difference was statistically significant(χ2=10.612,P<0.05).The coincidence rate of HPV subtypes in couples was 15.04％(37/246)in initial detection,while 8.94％(22/246)in redetection,the difference was statistically significant(χ2=4.333,P<0.05).The frequency percentage of HPV16,18 increased significantly in male,the difference was statistically significant(χ2=35.991,P<0.05).Conclusion HR-HPV can be cross-transmitted between infected women and their spouses.It is necessary to detect for her husband once women have been detected high-risk HPV positive.

Sepsis is defined as that a variety of pathogenic microorganisms(including bacteria,fungi,viruses and protozoa) invade the blood circulation,produce toxins and cause systemic infection.The early symptoms of neonatal sepsis are atypical.Neonatal sepsis is urgent and progresses quickly,especially in preterm infants,and it is easy to prone to septic shock which is life-threatening.Therefore,early identification,accurate diagnosis,and active intervention for neonatal sepsis and septic shock is essential to reduce mortality and improve prognosis.