Objective To study the clinical and MRI features of central pontine myelinolysis after liver transplantation.Methods 3 cases of orthotopic liver transplantation patients,displaying unconsciousness,drowsiness,reduced muscle strength of both lower extremities 3 to 21 days after the operation respectively,went through MRI scan in 2 days.Results MRI showed that symmetric long T1 and T2 signals,high-signal-intensity DWI and low-signal-intensity ADC were found in central pontine region.The cerebral cortex was involved in 2 cases,and abscess in basal ganglia appeared in 1 case.MRI examination showed that 2 cases had decreased lesion range,clear boundary and equal-signal-intensity DWI 35 days and 2 months respectively after.Conclusion MRI is valuable for the diagnosis of the patient with central pontine myelinolysis after liver transplantation.

Objective To investigate the effect of low intensity microwave electromagnetic fields (EMF) on energy metabolism of cerebral cortical neurons of postnatal rats. Methods The cultured neurons were exposed to low intensity 900 MHz continuous microwave EMF (SAR=0.38-3.22 mW/g) 2 hours a day for 4 to 6 consecutive days. The activity of cytochrome oxidase(CCO) was taken as the index for energy metabolism level. Results The results of cytochemistry showed that compared to the sham-exposed, a significant decrease of cytochrome oxidase activity in exposed neurons(0.38-3.22 mW/g P

Objective To study the advantage of magnetic resonance imaging(MRI) in diagnosis of complications following liver transplantation.Methods The MRI appearances were analyzed retrospectively in seventy-eight patients with postoperative complications after liver transplantation which was confirmed by operation,hepatic biopsy,angiography and cholangiography.Results The symptoms of skin and sclera jaundice,fever and belly pain were found in sixty-four patients.Liver function was abnormal in laboratory tests.Fourteen were non-symptom with normal liver function.By analyzing the MRI images,the results were found including hepatic portal vein anastomosis stenosis(n=21),hepatic arterial anastomosis stenosis(n=10),inferior vena cava anastomosis stenosis(n=1),bile duct anastomosis stenosis(n=15),bile duct non-anastomosis stenosis(n=5),transplant rejection(n=17),hepatic carcinoma recurrence(n=9).Conclusion MRI can be revealed hepatic vessels and bile ducts stenosis and dilations;it's the best device in diagnosis of complications after liver transplantation.

Objective To investigate the survival, differentiation and action of neural stem cell transplanted into striatum of Parkinson disease rat model. Methods Neural stem cells were isolated and cultured. The character of self-renewing was confirmed by technique of single cell clone culture. The ability of multi-directional differentiation and specific antigen-nestin was showed by immunocytochemistry(ICC). PD rat models were established, and the DAPI-labelled neural stem cells were transplanted into striatum. The 6-HHDA induced rotary behavior was measured, distribution and migration of the cell were checked by fluoresce activating. ICC and HPLC are used to see its differentiation and action of secrete neurotransmitter dopamine(DA) and it’s metabolites. Results The cultured stem cell express nestin and have the ability of self-renewing and multi-directional differentiation. 6-HHDA induced rotation decreased after transplantation of NSC into the PD rat striatum(P

In the present study, the effect of fibroblast-mediated IL-2 gene therapy on immune and hematopoietic reconstitution was observed after syngeneic bone marrow transplantation (BMT) in the mice which had received high-dose chemotherapy. The NK activity , LAK activity and the proliferation of BMT were augmented significantly , while there were no effects on the formation of CFU-GM, CFU-MK and CFU-E of bone marrow after IL-2 gene therapy. The results suggested that fibroblast-mediated IL-2 gene therapy can accelerate and prompt the process of immune reconstitution, then enchance the antitumor effect and reduce the complication such as infection after BMT. The experiment provide basis for the application of IL-2 gene therapy in BMT in the future.

In the present study, we prepared cationic liposomes encapsulated IL-2 DNA or IL-6 DNA with reverse-phase evaporation to investigate their efficiency in mediating gene transfer. After transfection with IL-2 DNA liposome or IL-6 DNA liposome, the supernatants of NIH3T3 cell contained high levels of IL-2 or IL-6. The peritoneal cells from the mice injected i.p. of IL-2 DNA or IL-6 DNA liposome 10 days later could also be detected to secrete IL-2 or IL-6. The B16F10 melanoma cells from tumor-bearing mice which were injected intratumorally of IL-2 DNA or IL-6 DNA liposome exhibited resistant to G418 and secreted high levels of IL-2 or IL-6. The results show that the liposome can mediate cytokine gene transfer efficiently in vitro and in vivo.

In the present study, the effects of G-CSF gene therapy was investigated on the recovery of murine hematopoietic suppression induced by high dose of cyclophosphomide (Cy) . The results showed that G-CSF gene therapy could slow down the Cy-induced decreasing of peripheral WBCs and platelets, and accerelate their recovery.It also could increase the CFU-GM, CFU - MK, CFU-S derived from the splenocyte and bone marrow cells in the chemotherapy-treated mice. The data demonstrated that fibroblast-mediated G-CSF gene therapy could significantlyreduce the hematopoietic damage to less extent, and accerelate hematopoietic recovery after chemotherapy.

Neutrophils play important role in anti - tumor response of tumor-bearing host as a kind of important effector cells. In order to identify the anti-tumor mechanisms of G-CSF gene therapy, we investigated the number and functions of the peripheral neutrophils in the C-26 colon adenocarcinoma-bearing mice receiving G - CSF gene therapy and high - dose chemotherapy. After G - CSF gene therapy, the number of neutrophils in peripheral blood was increased markedly in C-26 mice receiving high - dose 5 - Fu as compared with control groups including in vivo administration of rhG - CSF. The more potent cytotoxicity to C - 26 cells could be detected. The phagocytic activity, the secretion of IL-1, TNF, NO of the neutrophils were significantly enhanced. These data showed that G - CSF gene therapy can increase the number of neutrophils, activate the functions more effectively than in vivo administration of rhG - CSF.

Granulocyte colony - stimulating factor (G - CSF) is a hematopoietic growth factor that is responsible for the differentiation and proliferation of hematopoietic progenitor cells to mature granulocyte, and can increase the number of peripheral neutrophils. It has been demonstrated that it could inhibit the metastasis of the murine tumors in spontaneous and experimental metastasis models by in vivo administration of recombinant human G - CSF. In order to examine the antitumor effect of G - CSF gene therapy on mice receiving high - dose chemotherapy, C - 26 colon adenocarcinoma - bearing mice which were prepared by S. c. injection of 1?105C-26 cells were i. p. injected with rhG-CSF (2?g/day?14day) or implanted with 1?107 collagen encapsulated NIH3T3-G-CSF cells which secrete high level of G - CSF after gene transfection. In our experiment, rhG-CSF could inhibit the tumor growth and extend the survival time of early stage C-26 bearing mice. However, G - CSF gene therapy could inhibit the tumor growth and prolong the survival both in early or middle stage C-26 mice. The results showed that both rhG - CSF and G - CSF gene therapy have exact antitumor effect and G - CSF gene therapy show more effective than rhG - CSF in vivo. Then we investigated the therapeutic effects of G - CSF gene therapy on C-26-bearing mice receiving high - dose chemotherapy (5-Fu 150mg/mice i. p.) . More effective results could be observed in C-26 - bearing mice receiving high dose chemotherapy after G - CSF gene therapy. The results also suggested that G-CSF gene therapy can inhibit the tumor growth more effectively both in C-26-bearing mice or C-26-bearing mice receiving high - dose chemotherapy.

The human Interleukin-2 cDNA containing full-length of encoding region was cloned by RT-PCR from PBMNC and confirmed by DNA sequencing. The hIL-2 recombinant retroviral expressing vector (pLXSN-hIL2) was constructed by inserting hIL-2 cDNA into the BamHI cloning site of pLXSN retroviral vector. After packaged with CRIP packaging cell line, the hIL-2 retrovirions were produced at the titer of 7.6?105CFU/ml. Then a fibroblast cell clone(NIH3T3-hIL2) secreting 118.2U/ml hIL-2 was obtained by infecting the NIH3T3 fibroblast cells with hIL-2 retrovirions. The integration of hIL-2 provirus into the genome of NIH3T3-ML2 cells was confirmed by PCR analysis for NeoR gene. Our data showed that the hIL-2 retroviral vector was successfully constructed, which enables us to further the investigation of the hIL-2 gene therapy in clinical trial.