ObjectiveTo investigate the effect of repaglinide combined with insulin glargine in the treatment of patients with type 2 diabetes.Methods110 patients with type 2 diabetes were randomly divided into the control group and observation group,each group 55 cases,The control group was given novolin 30R,initial dose of 0.4U ·kg-1 · d-1.The observation group was given subcutaneous injection of insulin glargine at bedtime,the initial dose of 0.2U · kg- 1 · d - 1,preprandial oral repaglinide,3 of 6mg/d,divided into three doses,two groups were observed for 3 months,the difference of fasting plasma glucose (FPG),2h postprandial glucose (2hPG),glycated hemoglobin (HbAlc),Homa-IR,Homa-β,hypoglycemia,blood glucose time were compared before and after treatment.ResultsAfter treatment,FPG,2hPG,HbA1c,Homa-IR were significantly lower than before treatment,and Homa-β was significantly higher than that before treatment ( all P ＜ 0.01 ) ; After treatment,HbA 1 c,Homa-IR were lower than the control group significantly( t =2.786,3.449,all P ＜ 0.01 ),and Homa-β was significantly higher than the control group( t =-4.833,P ＜ 0.01 ); The blood glucose compliance time of the observation group was significantly shorter than the control group(t =2.126,P ＜0.01 ) ;The observation group had 0 case of low blood sugar,and the control group had 4 cases of low blood sugar,the difference was statistically significant( x2 =4.151,P ＜ 0.05 ).ConclusionRepaglinide combined with insulin glargine in the treatment of patients with type 2 diabetes could quickly reduce blood sugar,reduce blood glucose to normal levels,keep blood sugar stable,restore [β-cell function,reduce insulin resistance,reduce insulin dosage,reduce adverse events,and it was worthy of clinical use.

Objective To explore the effect of aerosol inhalation with pulmicort and terbutaline on children with asthma. Methods Sixty children infants with asthma were divided randomly into the treatment and control groups:the latter received routine treatment and nursing and the former were intervened with aerosol inhalation with pulmicort and terbutaline besides the regular nursing. The curative effect was compared between the groups.Result The total effectiveness rate(93.8%)in the treatment group was significantly higher than that(75.0%)in the control(χ2=4.273,P=0.044)group.Conclusions Aerosol inhalation with pulmicort and terbutaline may be effective in the treatment of asthma in infants.The nursing points like keeping a comfortable position,right dosage of the medicine and close observation of the diseases are critical for the effectiveness and safety during aerosol inhalation.

Objective To observe the clinical efficacy of electroacupuncture plus ginger-partitioned moxibustion in treating sudden hearing loss. Method Sixty patients with sudden hearing loss were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by electroacupuncture plus ginger-partitioned moxibustion, while the control group was by electroacupuncture alone. The audiometry was conducted before and after intervention, and the therapeutic efficacy was also evaluated.Result Respectively after the first and second treatment course, the threshold of audibility was significantly changed in both groups (P<0.05); there were significant differences in comparing the threshold of audibility between the two groups (P<0.05). The total effective rate was 80.0% and 90.0% respectively after the first and second treatment course in the treatment group, versus 53.3% and 63.3% in the control group, and the differences were statistically significant (P<0.05).Conclusion Electroacupuncture plus ginger-partitioned moxibustion is an effective approach in treating sudden hearing loss.

OBJECTIVE: To detect whether there is mixing of norfloxacin in loperamide hydrochloride capsules by UPLC-MS. METHODS: The separation of norfloxacin was performed on C18 column with column temperature set at 35 ℃, and the mobile phase was composed of acetronitrile and 0.1% formic acid (gradient elution) with a flow rate of 0.3 mL?min-1. The sample size was 10 ?L. ESI source was applied with capillary tube tension at 3.0 kV and ion source temperature at 110 ℃, and scan mode was multiple reaction monitor. RESULTS: It has been proved that there was norfloxacin in 2 of the total 5 batches of samples based on the data of retention time of RP-HPLC including ultraviolet spectra, the quasi-molecular ion and the second order mass spectra split fragments. CONCLUSION: The method is highly selective and sensitive, and can be used to detect whether norfloxacin has been illegally added into loperamide hydrochloride capsules.

Two kinds of incorrect cultivation modes were existed since the launch of cultivation for professional degree postgraduate of clinical medicine:taking professional degree postgraduates as scientific degree postgraduates to cultivate or taking scientific degree postgraduates as professional degree postgraduates to cultivate.In this paper,taking the cultivation of oncologic professional degree postgraduates as example,we put forward some suggestions:strengthening clinical skill training,promoting thinking mode of evidence-based medicine and enhancing clinical trial and statistical training.

Objective To analyze the examination results of external quality assessment(EQA),at all levels of iodine deficiency disorders(IDD) net work laboratories in Ningxia from 2002 to 2012,and to provide a reliable laboratory quality assurance for surveillance and control of IDD.Methods The data of IDD network laboratories at Ningxia provincial,municipal and county levels from 2002 to 2012 were collected and analyzed.Results Both of the response rate and qualification rate of salt iodine and urinary iodine laboratories at the provincial level were 100％ in the past 11 years.The response rate and qualification rate of salt iodine laboratories at municipal level were also 100％ ; the response rate of urinary iodine was 100％ since 2005,and the qualification rate had been 100％ for six years since 2007.The response rate of salt iodine at county level had been 100％ since 2004,and the qualification rate had been 100％ for four years since 2009.Conclusions IDD network laboratory in Ningxia Province runs good,and the quality control measures are solid and effective.

Purpose:To investigate the effect of in vitro antisense oligodeoxynucleotide of cyclin D 1 on the cyclin D 1 gene expression and cell proliferation of human stomach adenocarcinoma cell BGC 823 cell line.Methods:Phosphorothioate cyclin D 1 ASODN and random oligodeoxynucleotide (RODN) were synthesized and transfected into BGC 823 Cells. Their effects on cell proliferation were examined by MTT method,RT PCR method,immunohistochemical study.Results:Cyclin D 1 ASODN could significantly inhibit the growth of BGC 823 Cell lines.The RODN showed no such effect.The inhibition peaked at 48 hour after transfection by MTT method and was dose dependent.ASODN could downregulate the expression levels of cyclin D 1 mRNA and protein by RT PCR method and immunohistochemical study respectively.Conclusions:The data suggested that ASODN could specifically inhibit the expression of cyclin D 1 mRNA and protein and regulate cell cycle and cell proliferation of BGC 823 cells. [

OBJECTIVE: To study the bacterial endotoxin test for detecting pyrogen in fructose sodium diphosphate injection . METHODS: The interference of fructose sodium diphosphate injection to the process of bacterial endotoxin test was studied .RESULTS: Fructose sodium diphosphate injection in 10- fold dilution had no interference to the bacterial endotoxin lest .CONCLUSION:Bacterial endotoxin test could be used for pyrogen test of fructose sodium diphosphate injection.

Objective To assess the efficiency of central urban and rural hospitals in the 7 cities, counties and districts under the jurisdiction of Hangzhou City and the status of the medical markets so as to provide theoretical basis for the promotion of decentralized medical markets. Methods On the basis of defining the scope of a medical market, the HHI values of the medical markets in places where 37 hospitals in Hangzhou were located was calculated, the average efficiency of the various hospitals was measured by means of DEA, and an analysis was made on the correlation between the average efficiency and the HHI values. Results The central urban and rural medical markets in the various places surveyed, whose HHI values were all greater than 1 800, belonged to markets of monopolistic competition. Of the 37 hospitals, there were 11 whose relative efficiency reached 100% and another 11 whose relative efficiency was less than 60%. The greater the monopoly of a medical market, the lower the average efficiency of its hospitals. Conclusion Establishing competitive markets with low market concentration is an effective way of enhancing overall hospital efficiency.

OBJECTIVE:To introduce the experience of application and report for accreditation of drug clinical trial institution qualification of our hospital,to provide a reference for other hospitals. METHODS:From the building of institution,drug clinical trial ethics committee,clinical trials of listed drugs,simulated qualification accreditation,and other aspects,the experience and key points of application and report for accreditation of drug clinical trial qualification of our hospital were summed up. RESULTS& CONCLUSIONS:Priority should be given to staffing(particularly the leaders of the institution),the installation of software and hardware (for system development,training,institution office and specialized department),and the building of ethics committee. Departments should share work experience of clinical trials of listed drugs and set up a self-inspection group to simulate qualifica-tion accreditation. Work procedures and methods of qualification accreditation should be clearly understood,the requirements of Good Clinical Practice,the standards for qualification accreditation,should be known well;Good Clinical Practice should be seri-ously implemented and the subjects’rights and interests should be protected,which will contribute to the successful completion of application and report for qualification accreditation.