Objective To evaluate the impact of family intervention on the quality of life in the patients after total laryngectomy and their family members. Methods Family intervention was implemented for the patients after total laryngectomy and their family members from 2008 to 2009,and only patients received intervention from 2005 to 2007. The patients and their family members were investigated with Quality of Life Instrument for Cancer Patients-Head and Neck Cancer (QLICP-HN) and Quality of Life Scale-Family Version (QOL Scale-FAMILY) respectively when the patients were admitted to hospital and one year after discharged. Results The quality of life in patients after total laryngectomy and their family members was significantly improved after family intervention was implemented (P<0.05). Conclusion Family intervention is practicable and helpful to improve the quality of life in patients after total laryngectomy and their family members.

Dextranase can degrade dextran polymer into low molecular weight polysaccharide. Dextranase and its hydrolysates are widely used in food, medicine and chemical industries. Studies on dextranase progresses rapidly in recent years. We reviewed literature reports combined with our study about the progress of dextranase and its potential applications in industry. In addition, we addressed hot topics and emphasized on the current research about dextranase, existing problems in domesticstudies and the future research needs needs.
Dextranase ; chemistry ; Dextrans ; chemistry ; Molecular Weight ; Polymers

Objective To investigate the magnetic resonance imaging (MRI)features and pathologic characteristics of the fungal ball (FB)of the nasal sinuses,for the purpose to provide reference for surgical treatment.Methods The MRI data of 27 patients of nasal sinuses who were diagnosed by surgical pathology were retrospectively analyzed.Of 27 patients,there were 14 male and 13 female,aged from 49 to 86,anverage age 57.6,with course of disease from 6 months to 5 years.All patients preoperatively recieved 1.5 T high field strength mri scan and enhanced scan and functional nasal endoscopic surgical radical resection lesions.Results Maxillary sinus disease,which unilateral 92%(25 /27),bilateral 4% (1 /27),sphenoid sinus disease 4%(1 /27),FB was located in the sinus cavity central.FB isointense signal (com-pared with the white matter of the brain),hypointense on T2WI,DWI (b value =1000)was mixed high and low signal,Gd DTPA enhanced no enhancement,with around mucosal thickening isointense signal,high signal intensity on T2WI,ring enhancement.The histopathology slices of 27 cases were found wrapped fungal hyphae,which confirmed for FB.The pathological basis of FB was fungal silk containing manganese (paramagnetic substances),FB calcium phosphate and calcium sulfate and other substances,due to the magnetic susceptibility effect of T2WI was low signal,DWI high and low mixed signal changes.Conclusion MRI can reflect the histopathological characteristics of FB,and it is of great value in diagnosis and differential diagnosis,as well as providing important imaging evidence for patients undergoing surgical treatment.

Course construction is the central part in the teaching innovation and an important indicator of the quality of a school teaching. Based on the emphatic course construction ,the department of immunology has carried out practices from the educational philosophy,teaching content,teaching methods,teaching staff and other aspects of the courses in the construction of immunology,and has got better results.

A 1 270 bp full-length cDNA fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the '3' and 5' ends of the incomplete expression sequence tag (EST) of hypoxanthine-guanine phosphoribosyltransferase of Schistosoma japonicum (SjHGPRT) were amplified by the anchored PCR with 2 pairs of primer that were designed according to the published incomplete SjHGPRT EST and the sequence of multiclone sites of library λgt1 1 vector. Sequence analysis indicated that this fragment, with an identity of 82% to hypoxanthine-guanine phosphoribosyltransferase ofSchistosoma mansoni (SmHGPRT), contained a complete open reading frame(ORF). The deduced amino acid sequence showed 83% identity to that of SmHGPRT. This fragment was cloned into the prokaryotic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE revealed that M of the recombinant protein was about 28 ku. Western-blot analysis showed that the recombinant protein was recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Mice vaccinated with recombinant protein revealed significant worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs of the female worms reduction percentage, compared with the controls. Taken together, the SjHGPRT full-length cDNA can be cloned and expressed in E. coli as a recombinant protein that elicited immunity against the challenge infection with Schistosoma japonicum, indicating its potential as a partia1 protection vaccine candidate.

A 1 270 bp full-length cDNA fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of hypoxanthine-guanine phosphoribosyltransferase of Schistosoma japonicum (SjHGPRT) were amplified by the anchored PCR with 2 pairs of primer that were designed according to the published incomplete SjHGPRT EST and the sequence of multiclone sites of library ?gt11 vector. Sequence analysis indicated that this fragment, with an identity of 82% to hypoxanthine-guanine phosphoribosyltransferase of Schistosoma mansoni (SmHGPRT), contained a complete open reading frame(ORF). The deduced amino acid sequence showed 83% identity to that of SmHGPRT. This fragment was cloned into the prokaryotic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE revealed that M of the recombinant protein was about 28 ku. Western-blot analysis showed that the recombinant protein was recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Mice vaccinated with recombinant protein revealed significant worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs of the female worms reduction percentage, compared with the controls. Taken together, the SjHGPRT full-length cDNA can be cloned and expressed in E.coli as a recombinant protein that elicited immunity against the challenge infection with Schistosoma japonicum, indicating its potential as a partial protection vaccine candidate.

Objective To compare the diagnostic validity between the delirium rating methods (confusion assessment method,CAM) and the nurse used delirium screening scale(nursing delirium screening scale,NU-DESC)for the old dementia patients. Methods Patients who met the criteria for DSM-IV dementia diagnosis,the CAM and the NU-DESC was used to assess at 0,2,4,6,8 weeks after admission,and the DSM-IV was used as the gold standard diagnosis criteria for delirium. To compare the diagnostic sensitivity, specificity and consistency between the two evaluation tools using the ROC curve analysis and diagnostic consistency test. Results The criticality value of the CAM to estimate dementia patients with delirium was 26,with sensitivity 0.97,specificity 0.82,and the Kappa of the consistent with DSM-IV diagnosis was 0.60;the criticality value of the NU-DESC to estimate dementia patients with delirium was 3,with sensitivity 0.81, specificity 0.77,and the Kappa of the consistent with DSM-IV diagnosis was 0.45. For mild and moderate dementia patients,the diagnostic validity of the two tools was closer;for the severe dementia patients,the diagnostic validity of the CAM was better than the NU-DESC. Conclusions The sensitivity and specificity of the CAM used in the dementia patients were higher,the CAM was better than the NU-DESC,and could be used to screen for senile dementia patients with delirium;the NU-DESC could be used to estimate the mild and moderate dementia patients.