Objective To investigate the effect of mifepristone on the expression of β-endorphin in the ectopic focus of adenomyosis.Methods The expression of β-endorphin was measured by the immunochemistry method.Results During proliferative phase and secretory phase,the β-endorphin of mild dysmenorrhea were higher than that of midrange dysmenorrhea(t=2.672,2.711,all P＜0.05),the β-endorphin of midrange dysmenorrhea was higher than that of severe dysmenorrhea(t=2.341,2.365,all P＜0.05),the difference was significant.After being cured by mifepfistone,the expression of β-endorphin in the ectopic focus of adenomyosis was higher than that of control group (t=2.478,2.356,all P＜0.05),still lower than that of normal endometrial tissue,(t=2.123,2.233,all P＜0.05),the difference was significant.Conclusion The expression of β-endorphin in the ectopic focus of adenomyosis decreased significandy accompanied with the aggravation of the dysmenorrhea,and the mifepfistone could upregulate the β-endorphin and relieve the dysmenorrhea.

Objective To investigate the effect and significance of cinobufagin on the expression of survivin of HHUA cells in the endometrial carcinoma cultured in vitro. Methods HHUA cells of endometrial carcinoma were cultured in vitro. After being intervened by cinobufagin with different concentration, the expression of survivin was measured by RT-PCR and the cellular apoptosis was tested by flow cytometry. Results In the control group: mRNA expression of survivin of HHUA cells in endometrial carcinoma was (1.22±0.23), cellular apoptutic rate was (2.31± 0.98)%; cinobufagin of 0.25 mg/ml didn't affect the survivin and cellular apoptotic rate (q=2.89, P>0.05: q=3.12, P>0.05) ; after being exposed to einobufagin of 2.5 mg/ml, the expression of survivin was (0.73± 0.26), and cellular apoptotic rate was (26.50± 6.36) %, with all of these data being different from the control group significantly (q=5.68, P<0.05; q= 10.23, P<0.05) ; the actions of cinobufagin of 25 mg/ml on mRNA expression of survivin and apoptotic rate were similar to cinobufagin of 2.5 mg/ml (q=3.49, P>0.05; q=4.35, P>0.05) . Conclusion Cinobufagin of 2.5 mg/ml inhibit the mRNA expression of survivin and proliferation of HHUA cells in endometrial carcinoma.

Objegtive To investigate the effect and significance of cinobufagin on the expression of CD44s in ovarian carcinoma 3AO cells cultured in vitro.Methods Ovarian carcinoma 3AO cells were cultured in vitro;after the intervention of tinobufagin with different concentration,the expression of CD44s was measured by RT-PCR,and the cellular apoptosis was tested by flow cytometry.Results Ovarian carcinoma 3AO cells expressed CD44S mRNA of 1.3±0.1 and the cellular apoptofic rate was(2.31±0.98)%:0.25 mg/ml cinobufagin did not affect CD44s mRNA and cellular apoptofic rate(P ＞0.05):when 2.5 mg/ml cinobufagin was intervened,the expression of CD44s mRNA was 1.0±0.1 and the cellular apoptotic rate Was(28.69±4.16)%,showing significant difference comparing with the control group of ovarian carcinoma 3AO ceils(P＜0.05):the intervention of 25 mg/ml cinobufagin was similar to that of 0.25 mg/ml cinobuhgin(P＞0.05).3AO cells.

Objective To observe the clinical effectiveness of manipulation combined psychological counse-ling in treating early perimenopausal depression.Methods 62 patients with early perimenopausal depression were selected,and randomized into the two groups according visiting sequence.The treatment group received manipulation combined psychological counseling therapy, and control group received fluoxetine therapy.The clinical effectiveness between two groups was compared.Results The total effective rate of the treatment and control group was 90.30%and 83.90%,respectively,there was significant difference between the two groups (P=0.707);the score of HAMD depression scale was (8.90 ±0.87)points vs (9.29 ±2.98)points post 6 weeks of therapy,and the score reduction rate was (53.74 ±2.86)points vs (51.95 ±15.03)points (t=-0.69,0.65;P=0.492,0.520,respectively).No obvious side adverse effect was seen in the treatment group,and nausea (5 cases),dizziness (4) and anorexia (7) was seen in the control group.Conclusion Manipulation combined psychological counseling therapy was similarly effective in treating early perimenopausal depression compared with fluoxetine,but associated with less adverse effect.

BACKGROUND:In rehabilitation therapy teaching, it is crucial to cultivate professional knowledge and clinical skil s of rehabilitation therapy for students. SOAP (Subjective, Objective, Assessment, Plan) is the international most commonly used and accepted in the medical records of problem oriented method. SOAP is used to develop clinical thinking ability, improve analysis, judgment and the ability of solving clinical problem for students. OBJECTIVE:To retrospectively analyze the composition, teaching benefit and problems of SOAP in China. METHODS:Published articles about SOAP note were searched from PubMed, Web of Science and China National Knowledge Infrastructure, and the articles were retrieved from database building to September 2014. The key words were“SOAP note, rehabilitation, teaching”in Chinese and English. We excluded the repetitive researches. RESULTS AND CONCLUSION:After screening, we included 20 articles in the review. SOAP note was helpful to promote students’ independent thinking, strengthened the standardization of the records, rehabilitation treatment, and developed the students’ clinical thinking and the ability to deal with the problem. SOAP note has several chal enges in the rehabilitation teaching, however, with the popularity of SOAP teaching concept, and deep research and gradual generalization of SOAP teaching methods, SOAP has a wide application prospect for the rehabilitation education in China.

Objective To assess the predicting value of homocysteine ( HCY), low density lipoprotein cholesterin (LDL-C) and carotid intima-media thickness (IMT) for women with coronary artery disease (CAD). Methods To choose 115 patients with CAD and 102 patients of non-CAD;Homocysteine, LDL-C and IMT were assayed respectively;comparison and correlation analysis were performed based on genders. Results The rates for hyperhomocysteinemia and High Lipoproteinaemia in female CAD patients are 59. 6% and 75.0%,respectively,while they are 69. 9% and 78.6% for male CAD patients,which are both remarkably higher than 23.0% ,26.7%, 22. 2% and 23.6% in non-CAD patients ( Ps ＜ 0. 05 ). ROC curve shows that the best diagnostic boundary point is 0.953 mm for female IMT and 1. 021 mm for male IMT. At the boundary point,the sensitivity,specificity,positive predictive value and negative predictive value are 83.7% ,91.4% ,95.7% and 63. 8% in female, much higher than 62.9%, 65.3%, 76.2% and 53.3% in male. Conclusion hyperhomocysteinemia, high lipoproteinaemia are the important risk factors for women with coronary artery disease;IMT might be used as the predictor of CAD, which have more advantages for female than for male patients.

Objective To establish a method to detect neuromyelitis optiea (NMO)-IgG in patients serum using indirect immunofluorescence assay (IFA). Methods The normal tissues (cerebellum/ midbrain, kidney and stomach) from C57 mice were cryosectioned onto microscope slides as detective substrate. For NMO-IgG detection, isolated serum from patient with NMO, multiple sclerosis (MS), optic neuritis or myelitis was incubated with the tissue sections on the slide at 4℃ overnight and subsequently incubated with a fluorochrome-cojugated lgG specific for human. For double immunostaining with aquaporius-4 (AQP4), the slides were incubated with primary antibody of AQP4 and secondary antibody of IgG-TRITC. Detection of NMO-IgG and its co-localization with AQP4 was analyzed using fluorescence microscope. Results All 182 serum samples from patients were tested using IFA. Some samples revealed a characteristic immunohistochemical staining of NMO-IgG in mouse CNS tissues, predominately in pia and subpia, and capillaries in white and grey matter in the cerebellum, midbrain, and spinal cord. Double immunostaining with AQP4 demonstrated the co-localization of NMO-IgG with AQP4. Conclusions We established an IFA using a substrate from C57 mouse cerebellum/midbrain, kidney and stomach tissue to detect NMO-IgG in patient serum. This method is specific and efficient in detection and may be useful in diagnosis and differential diagnosis of neuromyelitis optica.

Objective To summarize the features of multiple sclerosis (MS) in China. Methods The clinical manifestations and inducements of 413 MS patients are comprehensively analyzed using clinical statistical method. Other parameters are also analyzed by electromyography and brainstem auditory evoked potential. Results MS in China mainly invades young and middle-aged people, with a higher ratio in female than in male. MS was found mostly acute or subacute in onset and usually due to an inducement mainly consisting of common cold, fever, cyesis and fatigue. The first seen symptoms were various and complicated, but mainly showed different types of visual disturbance such as the acuity of vision decrease, constriction of visual field and diplopia. Limb paralysis, sensory abnormality and visual disturbance were found the commonest symptoms. Lhermitte’s sign was common. Paroxysmal symptoms as algospasm and seizure were relatively common. Electromyography and brainstem evoked potential showed that peripheral nervous system damage might occur in MS. Spinal cord and visual nerve were found the mostly invaded according to the clinical data. Conclusion The features of MS in China should be different from those in the western countries.

Objective TO explore the etiology and pathogenesis of multiple sclerosis. Methods The information of pa-tients from 413 MS was analyzed comprehensively by the clinical statistical methods. Results Common cold was the com-monest factor causing MS, and fever,fatigue and childbirth were also the common causes. Immunological disturbance exis-ted in MS. CD~+_4 cell decreased in most of MS patients, but the distribution of CD~+_8 cell was not regular. The level of sexhormones was abnormal in MS,but it was not the same in male and female. There were three patients having family histo-ry of MS. Conclusion Virus infection,sex hormones and heredity factors are all related to MS through facilitating immu-nological disturbance.

BACKGROUND:Treatment programs for low back pain are of great varieties, but there is stil no a definite treatment. Pressure biofeedback instrument is a tool to measure abdominal muscle activity, and its reliability in the treatment of low back pain is less reported. OBJECTIVE:To explore the reliability and feasibility of the pressure biofeedback instrument for assessment of low back pain. METHODS:Thirty patients with chronic low back pain were enrol ed and subject to four kinds of postures:posture A was prone position;posture B was supine position with flexion of the knee joints, posture C was supine position with unilateral flexion of the hip and knee joint, posture D was prone position with unilateral shoulder flexion. Intra-abdominal pressure values under the different postures were measured twice within a week in the same subject. Intraclass correlation coefficient (ICC) was used to assess the reliability. RESULTS AND CONCLUSION:Posture A had good test-retest reliability, and the value of ICC was 0.853 (95%confidence interval:0.691-0.930);posture B showed moderate test-retest reliability, and the ICC value was 0.751 (95%confidence interval:0.477-0.882);posture C also had moderate test-retest reliability, and the ICC value was 0.789 (95%confidence interval:0.557-0.900), posture D had good test-retest reliability, and the value of ICC was 0.892 (95%confidence interval:0.641-0.919). Therefore, the pressure biofeedback for evaluation of low back pain has good reliability, which can be used for the evaluation of low back pain.