Objective To investigate the clinical efficacy of mother-child reinforcing-reducing acupoint combination in treating migraine of liver yang hyperactivity type.Methods Forty patients with migraine of liver yang hyperactivity type were randomly allocated to treatment and control groups, 20 cases each. The treatment group received acupuncture by mother-child reinforcing-reducing acupoint combination and the control group, acupuncture with conventional acupoint combination. Headache symptoms were scored in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups.Results There was a statistically significant pre-/post-treatment difference in the headache symptom score in the two groups (P<0.05). There was a statistically significant difference in pre-/post-treatment headache symptom score difference value between the treatment and control groups (P<0.05). The total efficacy rate and the cure and marked efficacy rate were 90.0% and 75.0%, respectively, in the treatment group and 85.0% and 35.0%, respectively, in the control group. There was a statistically significant difference in the cure and marked efficacy rate between the two groups (P<0.05).Conclusion Mother-child reinforcing-reducing acupoint combination is an effective way to treat migraine of liver yang hyperactivity type.

Objective To explore the clinical effect and safety of Wenxin granule combined with metoprolol on arrhythmia in elderly patients with coronary heart disease (CHD).Methods 186 elderly CHD patients with arrhythmia were divided into 3 groups according to therapy:group A (n=62,treated with Wenxin granule),group B (n=60,treated with metoprolol) and group C (n=64,treated with Wenxin granule plus metoprolol).Clinical effect and adverse effect were retrospectively analyzed.Results The excellence rate and total efficiency rate were higher in group C than group A andB [53.1％ (34 cases) vs.32.3％ (20 cases),30.0％ (18 cases); 93.8％ (60 cases) vs.40.3％ (25 cases),40.0％ (24 cases) ; respectively,all P＜0.05].There were no significant differences in the incidence of adverse effect in the 3 groups [4.8％ (3/62),8.3％ (5/60) vs.8.3％ (5/64),all P ＞0.05].Conclusions Wenxin granule combined with metoprolol improves clinical effect,decreases premature beat and has a better safety for elderly CHD patients with arrhythmia,which is worthy of clinical application.

Objective To explore the clinical efficacy and safety of low dose fentanyl combined to ropivacaine in subarachnoid block for cesarean section.Methods Eighty patients with ASA physical status Ⅰ and Ⅱscheduled to undergo cesarean section for delivery were randomly allocated to receiving spinally mixture none fentanyl ( group R),5 μg fentanyl ( group F5 ), 10 μg fentanyl ( group F10 ), and 15μg fentanyl ( group F15 ), combined with 1%ropivacaine 1 ml and 10% glucose 1 ml respectively,after combined spinal-epidural anesthesia puncture was applied at the L2～3 interspace.Heart rate, blood pressure, the onset and recovery time of the sensory and motor block,time to the highest level and the highest level of sensory block, time to the maximal extent and the maximal extent of the motor block were recorded at regular intervals.The adverse effects were sought during and after surgery.Neonatal outcome was assessed using Apgar score.Results The recovery of sensory block was prolonged significantly in group F10 and group F15 than in group R and group F5.Conclusion Ropivacaine with 10μg fentanyl is safe and effective in subarachnoid block for cesarean section.

Objective To investigate the alteration in intercellular adhesion molecule (ICAM)-l mRNA expression on the surface of human vascular endothelial cells (HUVECs) induced by LPS stimulation and the effects of hypertonic-hyperoncotic solution (HHS) . Methods HUVECs were enzymatically isolated and cultured in RPMI 1640 culture medium at 37℃ for about 7 days when a monolayer of endothelial cells has grown. The primarily cultured HUVECs were mixed with LPS 100ng/ml and incubated at 37℃ for 30min, 1,4,8, 12and 24 h. 10%NaCl and 10% hydroxyethyl starch (Fresenius) were added to RPMI 1640culture medium. The HHS concentrations were 0.25% and 0.5% respectively. HUVECs were first incubated with either HHS (0.25% , 0.5% ) or isotonic solution for 10 min, 1h and 4h and then stimulated by LPS for 4h. The ICAM-1 mRNA was measured by reverse transcriptase-PCR. Results There was slight expression of ICAM-1 mRNA on the surface of HUVECs in normal condition. ICAM-1 mRNA expression began to increase at 1h exposure to LPS and reached peak value at 4h. Both 0.25% and 0.5% HHS could prevent the LPS-induced ICAM-1 mRNA up-regulation (P

Objective To investigate the effect of hypertonic-hyperoncotic solution (HHS) on CD11b expression on the surface of human neutrophils which are stimulated by lipopolysaccharide (LPS) in vitro. Methods Neutrophils were isolated from fresh peripheral venous blood of healthy volunteers aged 20-4oyr find incubated with LPS 100 ng/ml al 37℃ in 5% CO2 incubator for 15, 30, 60min, 4, 12 or 24h. HHS was prepared with 10%NaCl and 10% hydroxyethyl starch. Neutrophils were incubated with 0.25% or 0.5%HHS for 10min, 1h or 4h. Some of the HHS treated neutrophils were further subjected to LPS stimulation for 30 mm. CDllb on the surface of neutrophils were measured by using immunofluorescence and flow cytometry. Results CD11b expression on neutrophils increased significantly after being exposed to LPS for 15min (P

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To understand the antagomism of Clostridium butyricm and Bifidobacterium infants to v. cholera. Either Clostridium butyricum LCL166 or Bifidobacterium infants LCL172 or both of them co-cultured with V. cholera of, 0139, counting the number of V. cholera in constant intervals and performing statistics analysis. The inhibition results are probably same when combination of LCL166 and LCL172 were compared with LCL166 alone. The antagonism to V. cholera results mainly from Clostridium butyricum LCL166 which has the inhibition effect to V.cholera.

Objective To evaluate the reliability of transcranial color duplex sonography(TCCD) in the detection of stenosis of the middle cerebral artery(MCA).Method 168 patients with cerebrovascular di sease were underwent both cerebral angiography and TCCD examination.Result 18 stenosis of the MCA were diagnosed by angiography, 15 of the 18 stenosis were detected by TCCD. But 2 MCA stenosis diagnosed by TCCD were not proved by angiography. TCCD diagnosed MCA stenosis with sensitivity(83%) and specificity(88%) respectively.Conclusion MCA stenosis diagnosed by TCCD was better than by transcranial Doppler(TCD).

Objective To observe the effect of Tongguanluo capsule in treating oviduct obstructive infertility and analyze the potential mechanism. Methods 106 patients with oviduct obstructive infertility were randomly divided into the observing group and the control group, 53 cases of each group. The observing group was treated by Tongguanluo capsule and the control group was treated by Huoxue Huayu Capsule. The effects were compared after 4 weeks. Results For observing group and control group respectively, the re-opening rates were 97.0% and 75.9% (P

Objective To investigate the effect of local mild hypothermia on stress hormones in patients with acute cerebral hemorrhage.Methods 78 patients were randomly divided into two groups, mild hypothermia group (38 cases) and routine group (40 cases). Conventional treatment was used in both groups, and local mild hypothermia therapy was applied in mild hypothermia group. The concentrations of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosteroid (CS) and aldosterone (ALD) in plasma pretherapy and at 3rd, 7th day after treatment were measured. The scores of clinical neurological deficit prior and at 3rd week after treatment were observed.Results The levels of plasma ACTH, CRH, CS and ALD prior treatment both in mild hypothermia and routine group were higher than those in healthy control group (all P