To study and compare the protective effects of Cordyceps sinensis from different habitats on acute injury of hepatic tissue induced by carbon tetrachloride ( CCl4 ) . Methods:The rat model of acute injury of hepatic tissue induced by CCl4 was established. The biochemical indicators of alanine aminotranferease ( ALT) and aspartate aminotransferease ( AST) in the serum and superoxide dismutase ( SOD) and L-glutathione( GSH) in the liver tissue were detected, and the liver tissue HE staining was used for the histopathology determination. Results:Compared with those in the model group, the levels of AST and ALT in the serum were sig-nificantly reduced, the levels of SOD and GSH in the liver tissue were notably increased, and the necrosis of liver cells was improved in Cordyceps sinensis groups at low, medium and high dose from different habitats (P<0. 05), however, the differences between the two different habitats were not significant (P>0. 05). Conclusion: Cordyceps sinensis from Qinghai and Tibet shows some positive differences in the protective effect on the acute chemical liver injury induced by CCl4 in rats,however, the differences are not remarka-ble.

OBJECTIVE: To investigate the protection mechanism of volatile oil of Magnolia biondii Pamp. (VOMBP) against diabetic nephropathy in rats by observing its effects on level of soluble P-selectin (sP-selectin) in serum and expression of P-selectin in renal tissue. METHODS: Fifty SD rats were randomly divided into normal control group, untreated group, and low-, medium- and high-dose VOMBP-treated group. Diabetic nephropathy was induced in rats by intraperitoneal injection of 1% streptozotocin. Before and the 1st day, 4th, 8th and 12th week after the induction, random blood glucose (RBG) and 24-hour urinary micro-albumin were detected in different groups. At the 12th week, the rats were sacrificed to collect the blood samples and renal tissues. The contents of blood urea nitrogen (BUN) and serum creatinine (SCr) were detected and the pathological change in renal tissues was observed by light microscope; the level of sP-selectin was detected by enzyme-linked immunosorbent assay and the expression of P-selectin protein in renal tissues was measured by immunohistochemical method. RESULTS: Compared with the normal control group, RBG, 24-hour urinary micro-albumin, the contents of BUN, sP-selectin in serum and expression of P-selectin protein in renal tissue in the untreated group were significantly increased (P<0.01), the content of SCr was significantly decreased (P<0.01), and pathological change of renal tissues was also obvious. Compared with the untreated group, 24-hour urinary micro-albumin, the level of sP-selectin in serum and expression P-selectin protein in renal tissue of the three VOMBP-treated groups were all significantly decreased (P<0.01), and pathological change was lessened too. However, there were no significant differences among the three VOMBP-treated groups (P>0.05). CONCLUSION: VOMBP can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue.

Objective Supramolecular hydrogels were the hydrogels consisting of a solid 3D network with noncovalent bonds.Its unique properties such as biocompatibility,biodegradability,free sol-gel transformation and stable drug release ability make it widely exploited for various biomedical applications.This paper mainly focused on the use of supramolecular hydrogels in all types of biomedical application such as biosensor,cell culture,tissue engineering,gene engineering and drug delivery by research literature reviews.They hope that this focus review will contribute topromote the use of supramolecular hydrogels.

Objective To study the diagnostic value of imageology for pyelic carcinoma.Methods The accuracy of qualitative diagnosis and sensitivity were analyzed by comparing various imaging examinations in 19 patients with pathologically proved pyelic carcinomas.Results Irregular filling defect and hydronephrosis were seen in the renal pelvis and calyces on intravenous pyelography(IVP).On CT scan,when the tumor was small,soft tissue mass was in the renal pelvis and the calyces.When the renal parenchyma involved by tumors,the soft tissue masses with unhomogeneous density,liquefaction necrosis in the renal pelvis and the parenchyma were seen.On contrast-enhanced CT scan,enhancement from slight to moderate was found in the lesion.The lesions were detected by IVP in 9/12 cases,by BUS in 10/16 cases,by CT in 18/19 cases and by MRI in 3/3 cases.Conclusion IVP and CT are the first method of choice to diagnose the pyelic carcinoma,BUS can be used as a screening method.While in evaluating the tumor involving the parenchyma of the ren or distant metastasis,CT and MRI are superior to IVP.

Objective To explore the feasibility and efficacy of endoscopic-assisted thyroidectomy (EAT) in the treatment of benign thyroid nodules.Methods Sixty-five patients with one-side benign thyroid nodules were treated with EAT (EAT group,n =33) or conventional open thyroidectomy(control group,n =32) from Jan.To Dec.2013.The operation time,blood loss amount,volume of drainage,visual analog scores (VAS) for pain severity,occurrence of postoperative complications,evaluation of cosmetic,and hospital expense were recorded.Results The operation were performed successfully in patients of two groups.Blood loss volume was significantly less in EAT group (26.7 ± 23.1) ml than in control group ((45.2-± 28.9) ml,t =2.85,P ＜0.05).The volume of drainage in EAT group was (31.4 ± 8.1) ml,less than that in control group((83.6 ± 17.3) ml,t =15.66,P ＜ 0.05).The VAS at the day of 1 st,3rd after operation were (1.5-± 0.4) and (1.0 ±0.2) in EAT group was significantly lower than those in control group ((2.2 ±0.5) and (1.5 ±0.3) ;t =6.01,7.29;P ＜0.05).Satisfaction rate of cosmetic in EAT group was higher than control group (97.0％ vs 68.8％,x2 =9.02,P ＜ 0.05).Conclusion EAT is proved with safe and feasible in the treatment of benign thyroid nodules,which is with the advantage of minimally invasive and cosmetic results.

Objective To observe the Orem self-care mode in patients treated with Miccoli endoscopy assisted thyroidectomy .Methods Sixty-four patients treated with Miccoli endoscopy assisted thyroidectomy were randomly divided into control group ( 32 cases ) and observation group ( 32 cases ) , patients in control group were treated with routine nursing care and the observation group was treated with routine nursing care plus Orem self-care mode.Mastering nursing knowledge , actively participating in nursing and the inpatients ’ satisfaction degree of nursing were compared in two groups .Results Rates of mastering nursing knowledge , actively participating in nursing , and satisfaction of nursing in observation group were 84.4%, 75.0%, 93.8%, higher than the control group, the differences were significantly (χ2 =7.273,10.656,9.143, respectively;P<0.01). Conclusions By using Orem self-care mode patients with Miccoli endoscopy assisted thyroidectomy can effectively master nursing knowledge , actively participate in nursing and improve the satisfaction .