Objective To observe application of optimized nursing in myocardial infarction patients with percutaneous coronary intervention(PCI).Methods The data of 80 acute myocardial infarction patients with PCI in our hospital were retrospectively analyzed.The patients were divided into observation group and control group according to time of admission.And 47 patients in observation group were treated with high quality nursing care, and 33 in the control group with regular health care.Vascular hemodynamics index, postoperative complications, hospitalization days, Barthel index, SAS and SDS scores were observed.The clinical effect of different healthcare modes was evaluated.Results Compared with the control group, the LVEDD of the observation group was (52.8±1.7) mm, which was lower than (54.2±1.6) mm in the control group, EF in observation group was (54.0±1.8)%, which was significantly higher than (48.3±1.4)% in control group and the differences were statistically significant (P<0.05).The incidence rate of adverse reactions in the observation group was 12.76%, which was significantly lower than 30.30% in the control group.At the same time, the hospitalization time of the observation group was significantly shorter than that of the control group.After treatment, the Barthel indicators of the observation group was higher than that of the control group, and the SAS and SDS scores in the observation group were lower than that in the control group (P<0.05).Conclusion For PCI patients with myocardial infarction during perioperative period, strengthening nursing care can effectively improve the treatment efficiency, promote physical and psychological rehabilitation.

Objective To observe application of optimized nursing in myocardial infarction patients with percutaneous coronary intervention(PCI).Methods The data of 80 acute myocardial infarction patients with PCI in our hospital were retrospectively analyzed.The patients were divided into observation group and control group according to time of admission.And 47 patients in observation group were treated with high quality nursing care, and 33 in the control group with regular health care.Vascular hemodynamics index, postoperative complications, hospitalization days, Barthel index, SAS and SDS scores were observed.The clinical effect of different healthcare modes was evaluated.Results Compared with the control group, the LVEDD of the observation group was (52.8±1.7) mm, which was lower than (54.2±1.6) mm in the control group, EF in observation group was (54.0±1.8)%, which was significantly higher than (48.3±1.4)% in control group and the differences were statistically significant (P<0.05).The incidence rate of adverse reactions in the observation group was 12.76%, which was significantly lower than 30.30% in the control group.At the same time, the hospitalization time of the observation group was significantly shorter than that of the control group.After treatment, the Barthel indicators of the observation group was higher than that of the control group, and the SAS and SDS scores in the observation group were lower than that in the control group (P<0.05).Conclusion For PCI patients with myocardial infarction during perioperative period, strengthening nursing care can effectively improve the treatment efficiency, promote physical and psychological rehabilitation.

Objective To explore the application effect of modified disinfection posture in coronary artery bypass grafting(CABG).Methods A total of 50 patients who would undergo CABG were recruited in this study by purposive sampling method in Xuzhou Central Hospital from January 2014 to December 2015. They were divided into the control group(25 cases,conventional disinfection posture)and the observation group (25 cases,modified disinfection posture)according to the random number table. The central venous pressure (CVP),blood pressure(BP)and heart rate(HR)were monitored between two groups before and after placing disinfection position with leg-raising immediately and after restoring horizontal position immediately. The comfort of body,the convenience of operation,the time of placing position and disinfection were investigated or recorded during the process of disinfection.Results There was no statistically significant difference in the time of placing position and disinfection during surgery between two groups(P>0.05). There was also no statistically significant difference in the comfort and satisfaction degree of doctors during surgery between two groups(P>0.05). The hemodynamic variables had no significant difference in two groups before leg-raising immediately(P>0.05). After leg-raising immediately,the systolic pressure,diastolic pressure,CVP and HR in the control group [(121.313±4.116), (70.423±3.291),(8.524±0.283) mmHg,(84.193±3.682) times/min] were higher than those in the observation group [(116.323±6.242),(66.191±11.468),(7.254±0.989) mmHg,(67.524±3.193) times/min] with significant differences(t=2.013,2.316,5.913,16.205;P<0.05). After restoring horizontal position immediately,the systolic pressure,diastolic pressure,CVP and HR in the control group [(109.267±6.167),(60.766±5.233),(4.063±0.572) mmHg, (86.524±4.254) times/min] were higher than those in the observation group [(101.274±9.156),(65.352±3.254), (3.544±0.623) mmHg,(69.933±7.424) times/min] with significant differences(t=3.450,2.025,2.705,9.266;P<0.05).Conclusions The application of modified disinfection posture in patients with general anesthesia and CABG has no effect on the comfort of body,the convenience of the operation,and it will not increase the time of placing position and disinfection. The modified disinfection posture can effectively avoid the impact of conventional disinfection posture on the hemodynamic variables and can improve the quality of nursing care.

Objective:To establish a competing risk model to predict the cumulative hazard risk probability of the outcomes (unhealed or hyperthyroidism recurrence) of Graves disease (GD) treated with 131I. Methods:From January 2020 to May 2021, 61 GD patients (13 males, 48 females; age (46.0±13.8) years) who received 131I treatment in Zhangzhou Affiliated Hospital of Fujian Medical University were enrolled. The outcomes of treatment were recovery, unhealed or hyperthyroidism recurrence (event 1), and hypothyroidism (event 2). Follow-up was started 1 month after 131I treatment and ended 1 year later. It was terminated in the following conditions: one of the two events occurred; no event occurred after 1 year of follow-up; the research deadline was up. The Fine-Gray test was used to analyze the factors related to event 1, and then the competitive risk model was established. Results:Thirty-nine patients had hypothyroidism, 17 patients were unhealed or had hyperthyroidism recurrence, 2 patients lost follow-up, and 3 patients had normal thyroid function after 1 year follow-up. Multivariate analysis showed that effective half-life (hazard ratio ( HR)=1.74, 95% CI: 1.10-2.75, β=0.55, P=0.019) and thyroid volume ( HR=1.12, 95% CI: 1.07-1.17, β=1.12, P<0.001) were risk factors for event 1, while the elasticity of thyroid was a protective factor ( HR=0.17, 95% CI: 0.06-0.54, β=-1.76, P=0.003). The C index of the nomogram constructed based on the multi-factor competitive risk model was 0.784(95% CI: 0.633-0.935). Conclusions:Thyroid volume, elastic value, and effective half-life are associated with treatment outcomes of 131I. The competitive risk model can predict the therapeutic outcomes of GD patients treated with 131I.

Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)8 triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of Caenorhabditis elegans. The activity of reAO-801 reached its peak at 40˚C and pH values between 4-7. Enzyme activity was inhibited by Zn2+, Ca2+, and Fe3+, whereas Mg2+ and K+ potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against C. elegans stage I larvae. reAO-801 broke down the C. elegans egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of Haemonchus contortus eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by A. oligospora.

DNA methylation is a prevalent epigenetic modification in vertebrates, and it has been shown to be involved the regulation of gene expression and embryo development. However, it remains unclear how DNA methylation regulates sexual development, especially in species without sex chromosomes. To determine this, we utilized zebrafish to investigate DNA methylation reprogramming during juvenile germ cell development and adult female-to-male sex transition. We reveal that primordial germ cells (PGCs) undergo significant DNA methylation reprogramming during germ cell development, and the methylome of PGCs is reset to an oocyte/ovary-like pattern at 9 days post fertilization (9 dpf). When DNA methyltransferase (DNMT) activity in juveniles was blocked after 9 dpf, the zebrafish developed into females. We also show that Tet3 is involved in PGC development. Notably, we find that DNA methylome reprogramming during adult zebrafish sex transition is similar to the reprogramming during the sex differentiation from 9 dpf PGCs to sperm. Furthermore, inhibiting DNMT activity can prevent the female-to-male sex transition, sug-gesting that methylation reprogramming is required for zebrafish sex transition. In summary, DNA methylation plays important roles in zebrafish germ cell development and sexual plasticity.